Reflection of electromagnetic radiation from plasma with an anisotropic electron velocity distribution

The reflection of a test electromagnetic pulse from the plasma formed as a result of tunnel ionization of atoms in the field of a circularly polarized high-power radiation pulse is analyzed using the kinetic approach to describe electron motion. It is shown that the reflected pulse is significantly amplified due to the development of Weibel instability. The amplification efficiency is determined by the maximum value of the instability growth rate, which depends on the degree of anisotropy of the photoelectron distribution function.     

Excitation of plasma waves by nonlinear currents induced by a high-frequency electromagnetic pulse

Excitation of plasma waves by nonlinear currents induced by a high-frequency electromagnetic pulse is analyzed within the kinetic approach. It is shown that the most efficient source of plasma waves is the nonlinear current arising due to the gradient of the energy density of the high-frequency field. Generation of plasma waves by the drag current is usually less efficient but not negligibly small at relatively high frequencies of electron–ion collisions. The influence of electron collisions on the excitation of plasma waves by pulses of different duration is described quantitatively.     

On the growth rate of aperiodic instability in plasma with an anisotropic bi-Maxwellian electron velocity distribution

The growth rate of aperiodic instability of transverse and longitudinal-transverse electromagnetic field perturbations in plasma with an anisotropic bi-Maxwellian electron velocity distribution is studied. The boundaries of the instability domains in wave vector space are found, and the growth rates of field perturbations with configurations different from that corresponding to the maximum growth rate are determined.     

Saturation of two-stream instability of an electron beam in plasma

The development and nonlinear saturation of two-stream instability of a warm nonrelativistic electron beam in a cold plasma are investigated numerically in the framework of a one-dimensional model. It is shown that, for a sufficiently large velocity spread of the electron beam, instability develops and saturates according to a universal law, the wave phase velocity remains the same in the saturation stage, and the maximum field is somewhat lower than that predicted by classical estimates and depends in a different way on the growth rate. The damping of plasma oscillations not only changes the instability growth rate, but also substantially decreases the maximum wave field.     

Dispersion properties of a plasma produced by a short X-ray pulse

A collisionless plasma produced by a short ionizing pulse from an X-ray laser is characterized by an anisotropic monoenergetic electron distribution governed by the classical photoeffect. The dispersion properties of such a photoionized plasma are studied. The spectra of high-frequency plasma waves and their damping, as well as the parameters of the aperiodic instability of a photoionized plasma, are described. The relationship between the electrostatic and magnetic perturbations generated by this instability is investigated, and an analysis is made of how the instability transforms into a purely longitudinal (two-stream-like) instability and into a purely transverse (Weibel-like) instability, depending on the absolute value and direction of the wave vector.     

Electromagnetic radiation from a plasma slab during the development of Weibel instability

Electromagnetic radiation from an anisotropic plasma slab formed by ionization of matter in the field of a high-power femtosecond pulse is studied. It is shown that the growth of initial field perturbations in the course of Weibel instability is accompanied by the generation of nonmonochromatic radiation with a characteristic frequency on the order of the instability growth rate. It is found that perturbations with characteristic scale lengths less than or on the order of the ratio of the speed of light to the Langmuir frequency are excited and radiated most efficiently, provided that the slab is thicker than this ratio.     

Amplification of surface waves in a plasma waveguide by a straight relativistic electron beam in a finite magnetic field

The problem of the excitation of electron waves in a thin-walled annular cold plasma in a cylindrical waveguide by a straight relativistic electron beam in a finite magnetic field is considered. The dispersion properties of a waveguide system with parameters close to the experimental ones are investigated. It is shown that the growth rate of the excited high-frequency plasma wave is comparable to that of the low-frequency wave, which is weakly sensitive to the strength of the longitudinal magnetic field.     

Theoretical and experimental investigations of the excitation of high-frequency oscillations in an ion collective accelerator model based on the Doppler effect

Results are presented from studies of a two-beam scheme of ion acceleration by a high-frequency field excited by an electron beam due to the instabilities associated with anomalous and normal Doppler effects. The dynamics of the excitation of eigenmodes in a periodic slow wave structure (SWS) by a relativistic electron beam via the anomalous Doppler effect is investigated theoretically. Mechanisms for the saturation of the instability are considered, analytical expressions for the maximum field amplitude and the efficiency with which the energy of beam electrons is converted into the energy of the excited wave are derived, and the results of numerical simulations of such excitation are presented. An experimental stand designed to test the principles and possibility of proton acceleration up to an energy of 8 MeV at a current up to 3 A is described. A double resonance (associated with anomalous and normal Doppler effects) occurring in the interaction of an electron beam with a helical SWS is studied experimentally. In this case, an increase in the efficiency with which the accelerating high-frequency field is excited is observed.     

Decay instability of a laser pulse propagating in a transverse direction in a magnetized plasma

The decay instability of a lser pulse propagating across an external magnetic field in a subscritical plasma is investigated analytically and numerically. It is shown that, when the relaxation of the pulse is taken into account, the hydrodynamic growth rate of the decay instability is slower than that obtained earlier in the constant-amplitude pump wave approximation. The results of numerical simulations by a particle-in-cell method demonstrate that an increase in the amplitude of the parametrically excited waves is accompanied by a decrease in their group velocity; in this case, up to 85% of the laser energy is converted into the energy of the plasma particles. It is found that, under resonance conditions, the magnetic field acts to increase the energy of the accelerated ions that escape from the plasma slab through its front boundary.     

Electrohydrodynamic effect resulting from high-frequency barrier discharge in gas

The formation of an electrohydrodynamic flow in atmospheric air by using a high-frequency barrier discharge distributed over the dielectric surface is investigated. The influence of variations in parameters of a fully solid-state pulse generator (with a peak voltage of 0–12 kV, a tunable repetition rate of 10–25 kHz, and a pulse duration of 7 μs) on the current of plasma ion emitter and velocity characteristics of airflow is considered.     

Multimode parametric instability during electron cyclotron heating

A study is made of the generation of electron Bernstein waves in the interaction of a microwave field with a magnetized plasma during electron cyclotron heating. Parametric resonance accompanied by simultaneous conversion of microwave-field energy into the energy of numerous waves is analyzed. The relevant dispersion relation is investigated using the Hill method, which has recently been applied for the first time to examine the parametric interaction between high-power microwave radiation and plasmas. It is shown that the dispersion relation can be used to describe the onset of modulational instability at multimode parametric resonance. The growth rate of the modulational instability is obtained. Efficient energy transfer from the microwave field into Bernstein modes and, accordingly, into plasma electrons may be one of the main mechanisms for electron cyclotron resonance plasma heating.     

Genetic transformation of intact Lactococcus lactis subsp. lactis by high-voltage electroporation

To apply recombinant DNA techniques for genetic manipulation of the industrially important lactococci, an efficient and reliable high-frequency transformation system must be available. High-voltage electric pulses have been demonstrated to enhance uptake of DNA into protoplasts and intact cells of numerous gram-negative and gram-positive microorganisms. The objective of this study was to develop a system for electroporating intact cells of Lactococcus lactis subsp. lactis LM0230 (previously designated Streptococcus lactis LM0230) with a commercially available electroporation unit (BTX Transfector 100; BTX, Inc., San Diego, Calif.). Parameters which influenced the efficiency of transformation included growth phase and final concentration of cells, ionic strength of the suspending medium, concentration of plasmid DNA, and the amplitude and duration of the pulse. Washed suspensions of intact cells suspended in deionized distilled water were subjected to one high-voltage electric pulse varying in voltage (300 to 900 V corresponding to field strengths of 5 to 17 kV/cm) and duration (100 microseconds to 1 s). Transformation efficiencies of 10(3) transformants per microgram of DNA were obtained when dense suspensions (final concentration, 5 x 10(10) CFU/ml) of stationary-phase cells were subjected to one pulse with a peak voltage of 900 V (field strength, 17 kV/cm) and a pulse duration of 5 ms in the presence of plasmid DNA. Dilution of porated cells in broth medium followed by an expression period of 2 h at 30 degrees C was beneficial in enhancing transformation efficiencies. Plasmids ranging in size from 9.8 to 30.0 kilobase pairs could be transformed by this procedure.     

Genetic transformation of intact Lactococcus lactis subsp. lactis by high-voltage electroporation.

To apply recombinant DNA techniques for genetic manipulation of the industrially important lactococci, an efficient and reliable high-frequency transformation system must be available. High-voltage electric pulses have been demonstrated to enhance uptake of DNA into protoplasts and intact cells of numerous gram-negative and gram-positive microorganisms. The objective of this study was to develop a system for electroporating intact cells of Lactococcus lactis subsp. lactis LM0230 (previously designated Streptococcus lactis LM0230) with a commercially available electroporation unit (BTX Transfector 100; BTX, Inc., San Diego, Calif.). Parameters which influenced the efficiency of transformation included growth phase and final concentration of cells, ionic strength of the suspending medium, concentration of plasmid DNA, and the amplitude and duration of the pulse. Washed suspensions of intact cells suspended in deionized distilled water were subjected to one high-voltage electric pulse varying in voltage (300 to 900 V corresponding to field strengths of 5 to 17 kV/cm) and duration (100 microseconds to 1 s). Transformation efficiencies of 10(3) transformants per microgram of DNA were obtained when dense suspensions (final concentration, 5 x 10(10) CFU/ml) of stationary-phase cells were subjected to one pulse with a peak voltage of 900 V (field strength, 17 kV/cm) and a pulse duration of 5 ms in the presence of plasmid DNA. Dilution of porated cells in broth medium followed by an expression period of 2 h at 30 degrees C was beneficial in enhancing transformation efficiencies. Plasmids ranging in size from 9.8 to 30.0 kilobase pairs could be transformed by this procedure.     

Sub-MHz bursts of nanosecond pulses excite neurons at paradoxically low electric field thresholds without membrane damage

Neuromodulation applications of nanosecond electric pulses (nsEP) are hindered by their low potency to elicit action potentials in neurons. Excitation by a single nsEP requires a strong electric field which injures neurons by electroporation. We bypassed the high electric field requirement by replacing single nsEP stimuli with high-frequency brief nsEP bursts. In hippocampal neurons, excitation thresholds progressively decreased at nsEP frequencies above 20–200 kHz, with up to 20–30-fold reduction at sub-MHz and MHz rates. For a fixed burst duration, thresholds were determined by the duty cycle, irrespective of the specific nsEP duration, rate, or number of pulses per burst. For 100-μs bursts of 100-, 400-, or 800-ns pulses, the threshold decreased as a power function when the duty cycle exceeded 3–5 %. nsEP bursts were compared with single “long” pulses whose duration and amplitude matched the duration and the time-average amplitude of the burst. Such pulses deliver the same electric charge as bursts, within the same time interval. High-frequency nsEP bursts excited neurons at the time-average electric field 2–3 times below the threshold for a single long pulse. For example, the excitation threshold of 139 ± 14 V/cm for a single 100-μs pulse decreased to 57 ± 8 V/cm for a 100-μs burst of 100-ns, 0.25-MHz pulses (p < 0.001). Applying nsEP in bursts reduced or prevented the loss of excitability in multiple stimulation attempts. Stimulation by high-frequency nsEP bursts is a powerful novel approach to excite neurons at paradoxically low electric charge while also avoiding the electroporative membrane damage.     

Interaction of an ion bunch with a plasma slab

Charge neutralization of a short ion bunch passing through a plasma slab is studied by means of numerical simulation. It is shown that a fraction of plasma electrons are trapped by the bunch under the action of the collective charge separation field. The accelerated electrons generated in this process excite beam?plasma instability, thereby violating the trapping conditions. The process of electron trapping is also strongly affected by the high-frequency electric field caused by plasma oscillations at the slab boundaries. It is examined how the degree of charge neutralization depends on the parameters of the bunch and plasma slab.     

Magnetorotational instability of a weakly ionized accretion disk with vertical and azimuthal magnetic fields

Magnetorotational instability of a weakly ionized accretion disk with an admixture of charged dust grains in a magnetic field with the axial and toroidal components is analyzed. The dispersion relation for perturbations perpendicular to the disk plane is derived with allowance for both the Hall current and the finite transverse plasma conductivity. It is shown that dust grains play an important role in the disk magnetic dynamics. Due to the effect of dust grains, the Hall current can reverse its direction as compared to the case of electron-ion plasma. As a result, the instability threshold shifts toward the short-wavelength range. Under certain conditions, electromagnetic fluctuations of any length can become unstable. It is established that the instability criterion for waves of any scale length is satisfied within a finite interval of the density ratio between the dust and electron plasma components. The width of this interval and the instability growth rate as functions of the plasma parameters and the configuration of the magnetic field in the disk are analyzed.     

Linear theory of electron cyclotron instability of electromagnetic waves in a magnetoactive plasma waveguide

The linear stage of electron cyclotron instability of quasi-TE modes in a waveguide filled with a magnetoactive plasma is studied using a kinetic approach. The dispersion relation of the instability is derived analytically. It is shown that the presence of the plasma can reduce both the linear instability growth rate and the instability region; in this case, the maximum of the growth rate is displaced toward lower frequencies. The results obtained are compared with the available experimental observations. They can be useful for optimizing the operating regimes of high-power continuous-wave gyrotrons.     

Electric pulse-mediated gene transfer in mammalian cells grown in suspension culture

A high-voltage generating machine which could generate semi-rectangular pulses in PBS solution was constructed, and the effects of field strength and duration of the pulse on electric pulse-mediated transformation of mouse mammary carcinoma FM3A cells by a linear form of plasmid pSV2neo DNAs were examined. In parallel, cell survival and growth after pulsing were analyzed. When the field strength and duration of the pulse were increased, the transformation frequency increased, although the cell survival rate decreased. Under the best conditions, the transformation frequency was 2 X 10(-4), which was 80 times higher than that obtained by the calcium phosphate coprecipitation method.     

Electropermeabilization of mammalian cells. Quantitative analysis of the phenomenon.

Transient membrane permeabilization by application of high electric field intensity pulses on cells (electropermeabilization) depends on several physical parameters associated with the technique (pulse intensity, number, and duration). In the present study, electropermeabilization is studied in terms of flow of diffusing molecules between cells and external medium. Direct quantification of the phenomenon shows that electric field intensity is a critical parameter in the induction of permeabilization. Electric field intensity must be higher than a critical threshold to make the membrane permeable. This critical threshold depends on the cell size. Extent of permeabilization (i.e., the flow rate across the membrane) is then controlled by both pulse number and duration. Increasing electric field intensity above the critical threshold needed for permeabilization results in an increase membrane area able to be permeabilized but not due to an increase in the specific permeability of the field alterated area. The electroinduced permeabilization is transient and disappears progressively after the application of the electric field pulses. Its life time is under the control of the electric field parameters. The rate constant of the annealing phase is shown to be dependent on both pulse duration and number, but is independent of electric field intensity which creates the permeabilization. The phenomenon is described in terms of membrane organization transition between the natural impermeable state and the electro-induced permeable state, phenomenon only locally induced for electric field intensities above a critical threshold and expanding in relation to both pulse number and duration.