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Measurement of saccharifying cellulase. 总被引:62,自引:0,他引:62
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Production of cellulase by Trichoderma. 总被引:6,自引:0,他引:6
D Sternberg 《Biotechnology and bioengineering symposium》1976,(6):35-53
The cellulase complex in T. viride is inducible. For large-scale enzyme production the fungus should be cultured on media containing cellulose. The cellulase enzymes are respressible. To produce and maintain best cellulase yields cultural conditions which lead to carbohydrate consumption in excess of cellular needs should be avoided. With the present mutant (QM9414) extracellular enzyme preparations having 1.6 FP units/ml and 1.6 mg protein/ml have been obtained within four to five days in submerged fermentation. Such preparations are capable of producing a 5% sugar solution when mixed with 10% ball milled cellulose and incubated 24 hr at 50 degrees C. Further improvements of cellulase yields are being sought by continued mutagenesis and increased nutrient levels in the growth medium. 相似文献
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A Cellulomonas sp. was isolated from the soil which hydrolyzed cellulose, as shown by clear-zone formation on cellulose agar medium. Catabolite repression of cellulase synthesis occurred when moderate levels of glucose were added to the medium. A stable mutant that no longer exhibits catabolite repression was produced through treatment of the wild-type organism with N-methyl-N'-nitro-N-nitrosoguanidine. Both enzyme concentration and specific activity, as determined by the rate of hydrolysis of carboxymethylcellulose, were greater with the mutant than with the wild-type organism under various test conditions. The wild type had no measurable cellulase activity when grown in the presence of either 1.0% glucose or cellobiose. Cellobiose, but not glucose, inhibited enzyme activity towards both cellulose and carboxymethylcellulose. Cellobiose, cellulose, and sophorose at low concentrations induced cellulase synthesis in both the wild-type and the mutant organism. Cellulase regulation appears to depend upon a complex relationship involving catabolite repression, inhibition, and induction. 相似文献
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G Okada 《Journal of biochemistry》1976,80(5):913-922
A cellulase [EC 3.2.1.4] component was purified from a crude cellulase preparation of Trichoderma viride (Meicelase) by consecutive column chromatography procedures, and was designated as cellulase III. The enzyme was homogeneous on polyacrylamide gel disc electrophoresis. The molecular weight of the enzyme was estimated to be about 45,000 by gel filtration. The optimum pH and temperature of the enzyme were pH 4.5-5.0 and 50 degrees, respectively. The enzyme was stable over the range of pH 4.5-7.5 at 4 degrees for 24 hr, and retained 40% of the original carboxymethylcellulose-saccharifying activity after heating at 100 degrees for 10 min. The enzyme was completely inactivated by 1 mM Hg2+, and partially by 1 mM Ag+ and Cu2+. The enzyme was characterized as a less-random type cellulase on the basis of its action on carboxymethylcellulose. The enzyme split cellohexaose, retaining the beta-configuration of the anomeric carbon atoms in the hydrolysis products. The Km values of cellulase III for cellooligosaccharides decreased in parallel with increase of the chain length of the substrates, while Vmax values showed a tendency to increase. The enzyme produced predominantly cellobiose and glucose from various cellulosic substrates as well as from higher cellooligosaccharides. Cellulase III preferentially attacked the aglycone linkage of p-nitrophenyl beta-D-cellobioside. The enzyme was found to catalyze the rapid synthesis of cellotetraose from cellobiose (condensation action). 相似文献
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Two highly purified cellulases [EC 3.2.1.4], II-A, and II-B, were obtained from the cellulase system of Trichoderma viride. Both cellulases split cellopentaose retaining the beta-configuration of the anomeric carbon atoms in the hydrolysis products at both pH 3.5 and 5.0. The Km values of cellulases II-A and II-B for cellotetraose were different, but their Vmax values were similar and those for cellooligosaccharides increased in parallel with chain length. Both cellulases produced predominantly cellobiose and glucose from various cellulosic substrates as well as from higher cellooligosaccharides. Cellulase II-A preferentially attacked the holoside linkage of rho-nitrophenyl beta-D-cellobioside, whereas cellulase II-B attacked mainly the aglycone linkage of this cellobioside. Both cellulases were found to catalyze the synthesis of cellotriose from rho-nitrophenyl beta-D-cellobioside by transfer of a glucosyl residue, possibly to cellobiose produced in the reaction mixture. They were also found to catalyze the rapid synthesis of cellotetraose from cellobiose, with accompanying formation of cellotriose and glucose, which seemed to be produced by secondary random hydrolysis of the cellotetraose produced. The capacity to synthesize cellotetraose from cellobiose appeared to be greater with cellulase II-B than with cellulase II-A. 相似文献
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Cellulases (EC 3.2.1.4) of a Cytophaga species WTHC 2421 (ATCC 29474) were found in the soluble portion of the cell (the periplasm and the cytoplasm) and on the membrane. Cell-free cellulases were not found. Most of the carboxymethylcellulase activity associated with reduction of viscosity was membrane bound, whereas most of the carboxymethylcellulose (CMC) saccharifying activity was soluble. The CMC-saccharifying activity was increased 534 X by purification procedures which included ammonium sulfate precipitation and molecular exclusion chromatography with Sephadex G-75 and Biogel p-100. Periplasmic carboxymethycellulase had a molecular weight of 6250 and cytoplasmic carboxymethylcellulase had a molecular weight of 8650. Analytical ultracentrifugation of the periplasmic carboxymethylcellulase (CMCase) indicated that it had a low molecular density. The chromatographic fraction containing periplasmic CMCase also contained enzyme activity against crystalline cellulose. The activity against crystalline cellulose was 238 X higher than the activity shown by the whole cell. The reaction of the enzyme with either CMC or dewaxed cotton produced only glucose. The enzyme was slightly inhibited by the presence of 0.01% (w/v) glucose, lactose, or cellobiose, but it was not affected by sucrose, and exhibited increased activity in the presence of xylose and fructose. 相似文献
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The mechanism of fungal cellulase action. Synergism between enzyme components of Penicillium pinophilum cellulase in solubilizing hydrogen bond-ordered cellulose. 总被引:1,自引:0,他引:1
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Studies on reconstituted mixtures of extensively purified cellobiohydrolases I and II and the five major endoglucanases of the fungus Penicillium pinophilum have provided some new information on the mechanism by which crystalline cellulose in the form of the cotton fibre is rendered soluble. It was observed that there was little or no synergistic activity either between purified cellobiohydrolases I and II, or, contrary to previous findings, between the individual cellobiohydrolases and the endoglucanases. Cotton fibre was degraded to a significant degree only when three enzymes were present in the reconstituted enzyme mixture: these were cellobiohydrolases I and II and some specific endoglucanases. The optimum ratio of the cellobiohydrolases was 1:1. Only a trace of endoglucanase activity was required to make the mixture of cellobiohydrolases I and II effective. The addition of cellobiohydrolases I and II individually to endoglucanases from other cellulolytic fungi resulted in little synergistic activity; however, a mixture of endoglucanases and both cellobiohydrolases was effective. It is suggested that current concepts of the mechanism of cellulase action may be the result of incompletely resolved complexes between cellobiohydrolase and endoglucanase activities. It was found that such complexes in filtrates of P. pinophilium or Trichoderma reesei were easily resolved using affinity chromatography on a column of p-aminobenzyl-1-thio-beta-D-cellobioside. 相似文献
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A new procedure for the determination of cellulase activity is described. The cellulases are incubated for 30 min at 39°C with finely divided cellulose at pH 6.9, and the glucose and cellobiose produced during the incubation are silylated and measured by gas-liquid chromatography. The precision of various steps in the procedure are determined and the optimum conditions for the enzyme assay are established. The coefficient of variation for the assay was 2.4 to 4.5%, depending on the substrate used. Although the method is specifically developed for the measurement of cellulase activity in mixed enzyme preparations from sheep rumen contents, it is applicable for the determination of other cellulases. 相似文献
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Summary Fluid immobilized cellulase was prepared using polyethyleneglycols and hexamethylene diisocyanate, and its properties studied. The cellulase activity of the immobilized enzymes varied with monomer composition and molecular weight of polyethyleneglycols. The enzyme activity was affected by the viscosity of the carrier. A solid substrate (cellulose powder) can be hydrolyzed with the fluid immobilized enzyme. 相似文献
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A procedure involving affinity chromatography on cellulose was developed for separating enzymic components of the cellulase complex. Cellobiase, carboxymethylcellulase, component C2 and cellobiohydrolase are adsorbed with increasing tenacity, and released, as highly purified components, as the ionic strength of the eluent is decreased. 相似文献
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纤维素酶的底物专一性 总被引:8,自引:0,他引:8
天然纤维素的有效酶解取决于外切葡聚糖纤维二糖水解酶(CBH)和内切葡聚糖水解酶(EG)的协同作用。EG随机水解纤维素无定形区分子链内的β-1,4-糖苷键;CBH则由分子链的还原性末端水解出纤维二糖。这种底物专一性差别的原因在于CBH呈“桶状”的活性部痊表面存在2个“loop”结构,只能容许纤维素分子链的末端伸入到活性裂隙中。EG无“loop”结构在存在,对底物是充分可及的。EG催化结构域中底物结合 相似文献
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Douglas E Eveleigh Mary Mandels Raymond Andreotti Charles Roche 《Biotechnology for biofuels》2009,2(1):21-8
This article sets forth a simple cellulase assay procedure. Cellulose is variable in nature, insoluble and resistant to enzymatic
attack. As a result there have been a bevy of bewildering cellulase assays published that yielded irrational results. Certain
protocols focused on the rapidity of the assay while ignoring that only the most readily susceptible cellulose regions were
being hydrolyzed. Other assays simplified the system by using modified soluble substrates and yielded results that bore no
relationship to the real world hydrolysis of insoluble cellulose. In this study Mandels, Andreotti and Roche utilized a common
substrate, Whatman filter paper. Hydrolysis of a 50 mg sample of the paper was followed to roughly 4% degradation, which circumvented
the problems of attack of only the most susceptible zones. This common hydrolysis target range also resulted in some balance
with regard to the interaction of the several cellulase components. The method was subsequently widely adopted. 相似文献