共查询到20条相似文献,搜索用时 15 毫秒
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E Aldová 《Journal of hygiene, epidemiology, microbiology, and immunology》1989,33(2):219-228
Seashore water samples collected along the coastline in Bulgaria and Rumania contained in large numbers OK serovars of V. parahaemolyticus; some of these had been isolated repeatedly over an extended time period: 01 K32, 03 K30, 03 K48, 04 K37, 04 K53, 05 K17, 05 K30. The serovar 05 K17 was virtually present in all water samples and was also isolated from a case of purulent ear infection in a child from Burgas. In contrast, strains recovered from Asian and African coastal water had different K antigens and were never identified in Europe. Two strains of V. parahaemolyticus (serovars 05 K15 and 07 K10) had positive swarming growth resembling that of V. alginolyticus. The first of these was Kanagawa-positive and was isolated from a case of severe diarrhea in Brazzaville. Vibrio parahaemolyticus isolates came from marine or brackish water specimens collected on sand banks, 3 strains were recovered from marine or brackish water in Africa. Vibrio harveyi, a sucrose-negative species important from differential diagnostic aspects, has been isolated from seashore water samples collected on coarse-sand or pebbly beaches. 相似文献
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McCarter L 《Journal of molecular microbiology and biotechnology》1999,1(1):51-57
Vibrio parahaemolyticus is a ubiquitous marine bacterium and human pathogen. The organism possesses multiple cell types appropriate for life under different circumstances. The swimmer cell, with a single polar flagellum, is adapted to life in liquid environments. The polar flagellum is powered by the sodium motive force and can propel the bacterium at fast speeds. The swarmer cell, propelled by many proton-powered lateral flagella, can move through highly viscous environments, colonize surfaces, and form multicellular communities which sometimes display highly periodic architecture. Signals that induce differentiation to the surface-adapted cell type are both physical and chemical in nature. Surface-induced gene expression may aid survival, whether attached to inanimate surfaces or in a host organism. Genetic rearrangements create additional phenotypic versatility, which is manifested as variable opaque and translucent colony morphotypes. Discovery that a LuxR homolog controls the opaque cell type implicates intercellular signaling as an additional survival strategy. The alternating identities of V. parahaemolyticus may play important roles in attachment and detachment, how bacterial populations adapt to growth on surfaces, form structured communities, and develop biofilms. 相似文献
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Pathogenicity and genome of Vibrio parahaemolyticus 总被引:1,自引:0,他引:1
Iida T 《Nihon saikingaku zasshi. Japanese journal of bacteriology》2004,59(3):457-464
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Induction of swarming in Vibrio parahaemolyticus 总被引:8,自引:0,他引:8
S Ulitzur 《Archives of microbiology》1974,101(4):357-363
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Production of pili on Vibrio parahaemolyticus 总被引:4,自引:0,他引:4
Electron microscopic examination showed that all strains of Vibrio parahaemolyticus examined had pili on their surface when the organism was grown on marine agar at 28 degrees C for 6-12 h. The pili were morphologically stable on heat treatment at 60 degrees C for 10 min, but both the lateral and polar flagella possessed by this organism were labile. No immunological cross-reactivity between pili of enterotoxigenic Escherichia coli and Vibrio cholerae non-01 and those of V. parahaemolyticus was observed. 相似文献
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Caburlotto G Knight IT Lleo MM Taviani E Huq A Colwell RR 《Systematic and applied microbiology》2011,34(8):617-620
Insignia is a novel DNA computational system which uses highly efficient algorithms to compare bacterial genomes and to identify specific DNA signatures to distinguish a target bacterium, or group of bacteria, from all other known bacterial species. It is currently being validated using different bacterial groups, including Vibrio spp. In this study, the genomic analysis by Insignia was conducted on Vibrio parahaemolyticus, a halophilic gram-negative bacteria which constitutes a leading cause of seafood-borne disease. Insignia was used to identify 37 V. parahaemolyticus-specific signatures and to design PCR assays to validate the representative signature sequences by TaqMan essays. The 37 assays targeted loci distributed around the genome and detected genes coding for hypothetical proteins and for proteins involved in adhesion, starvation and virulence. A panel of V. parahaemolyticus environmental strains isolated from the North Adriatic Sea (Italy) and from the Black Sea (Georgia) was used to validate the selected signatures. The signature assays revealed both sensitive and specific and the method allowed a more accurate identification of the tested bacterial strains at the species level when compared to biochemical and PCR standard methods. Using Insignia, it was possible to distinguish two different groups among the strains previously identified as V. parahaemolyticus: most of the strains were included in a "V. parahaemolyticus-like group" showing nearly all of the signatures assayed while a small group of 10 strains contained only a few of the signatures tested. By sequencing the 16S rDNA of this latter group, it was confirmed that they were not V. parahaemolyticus but in fact belonged to other Vibrio species. No significant genome-wide differences were detected between the strains isolated in Italy and in Georgia though the very different geographical origin. 相似文献
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A.B. Ellingsen H. Jørgensen S. Wagley M. Monshaugen L.M. Rørvik 《Journal of applied microbiology》2008,105(6):2195-2202
Aims: To examine the variability among environmental Vibrio parahaemolyticus (including trh+ isolates) from Norway, and to compare these to clinical isolates and isolates from imported foods. Methods and Results: A total of 246 V. parahaemolyticus were successfully digested with NotI, and the fragments were separated by pulsed field gel electrophoresis (PFGE). The isolates could be divided into 72 clusters and 103 pulsotypes. Eleven clusters contained 4–31 environmental isolates, and the isolates within these clusters greatly varied with respect to origin. None of the trh+ and /or tdh+ isolates clustered with trh?/tdh? isolates. The trh+ environmental isolates included in the study belonged to two separate clusters. A subset of isolates was serotyped, and great serotype diversity was observed among the environmental V. parahaemolyticus. The clinical isolates included O3:K6 and O3:KUT, and these were identical or related to a pandemic reference strain by PFGE. Conclusions: Environmental V. parahaemolyticus (including trh+) were genetically diverse, but certain variants occurred throughout the coastal environment, and some were persistent over time. Significance and Impact of the Study: Although trh+ V. parahaemolyticus persisted in the Norwegian environment, no evidence indicated that indigenous isolates have caused disease. 相似文献
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The antigenic relationships of 79 strains of Vibrio parahaemolyticus representing 46 assigned K-types were studied by tube agglutination. Homologous titers of 46 anti-K sera ranged from 80 to 2,560. All but three sera exhibited from one to six heterologous reactions, the majority of which gave titers of =20, but 19 sera showed cross-reactions whose titers exceeded 40. Nine reciprocal and 16 unilateral relationships were described. Some of the cross-reactions involved heat-extractable K-antigens, as determined by absorption with nonheated and heated heterologous antigens, whereas others did not involve K-antigens since absorption with the heterologous antigens had no effect on the homologous systems. On the basis of the reactions of selected antisera to the O-antigens of some of the K-strains, the cross-reactions could not be explained solely on the basis of O-specificities. 相似文献
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R Sakazaki 《Japanese journal of medical science & biology》1968,21(5):359-362
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Medium for Isolation and Differentiation of Vibrio parahaemolyticus and Vibrio alginolyticus 总被引:6,自引:4,他引:2 下载免费PDF全文
Miguel Kourany 《Applied microbiology》1983,45(1):310-312
Trypticase soy agar supplemented with sucrose, sodium chloride, bile salts, and triphenyltetrazolium chloride is an improved plating medium for the isolation of Vibrio parahaemolyticus from samples of seawater, permitting better differentiation of this organism from Vibrio alginolyticus and other bacteria. 相似文献
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J. S. Lee 《Applied microbiology》1972,23(1):166-167
Vibrio parahaemolyticus cells are readily inactivated in distilled water. The time of exposure required to inactivate 90% of the cells was between 0.9 and 4.4 min. 相似文献
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