假单细胞GX4—1利用鱼粉废水产絮凝剂的研究

研究了假单胞菌（Pseudomonas sp.) GX4-1利用鱼粉废水产生絮凝剂的条件,结果表明,适宜条件为废水COD浓度为10g／L左右、初始pH为7－9、培养温度为30℃、摇床转速为100－250r/min,此时的絮凝率可高达99．5％。同时发现在废水培养基灭菌的条件下,该菌仍能产生高效的絮凝剂。该菌利用鱼粉废水产生的絮凝剂对高岭土悬液、土壤悬液和细活性炭粉末悬液和电瓷厂污水均有较好的絮凝作用。     

假单细菌GX_4-1利用鱼粉废水产絮凝剂的研究

研究了假单胞菌 (Pseudomonassp .)GX4 1利用鱼粉废水产生絮凝剂的条件 ,结果表明 ,适宜条件为废水COD浓度为 1 0g/L左右、初始pH为 7～ 9、培养温度为 30℃、摇床转速为 1 0 0～ 2 50r/min ,此时的絮凝率可高达 99 5%。同时发现在废水培养基不灭菌的条件下 ,该菌仍能产生高效的絮凝剂。该菌利用鱼粉废水产生的絮凝剂对高岭土悬液、土壤悬液和细活性炭粉末悬液和电瓷厂污水均有较好的絮凝作用。     

微生物絮凝剂产生菌的筛选及培养条件的优化

目的:从好氧活性污泥、土壤和河泥中分离筛选具有较高活性的絮凝剂产生菌,并优化其培养条件;方法:通过常规分离获得目的菌株,运用单因素法考察培养时间、培养温度、发酵液初始pH值及摇床转速对菌株絮凝活性的影响;结果:得到了絮凝活性较高的菌株,经过优化得出,其最佳培养时间和温度分别为48h和30℃,发酵液最佳初始pH值为7.0,最佳摇床转速为120r/min,在最佳培养条件下,RF-32对高岭土悬浊液絮凝率为84.32%。结论:从活性污泥中可筛选出较高活性的絮凝剂产生菌,研究发现,菌株的絮凝活性与其生长量呈同步增长趋势,并在一段时间后达到一稳定值。培养时间、培养温度、发酵液初始pH值及摇床转速均能通过一定的作用因素对菌株生长情况产生影响,进而影响其絮凝活性。     

假单细菌GX4-1利用鱼粉废水产絮凝剂的研究

研究了假单胞菌 (Pseudomonassp .)GX4 1利用鱼粉废水产生絮凝剂的条件 ,结果表明 ,适宜条件为废水COD浓度为 1 0g/L左右、初始pH为 7～ 9、培养温度为 30℃、摇床转速为 1 0 0～ 2 50r/min ,此时的絮凝率可高达 99 5%。同时发现在废水培养基不灭菌的条件下 ,该菌仍能产生高效的絮凝剂。该菌利用鱼粉废水产生的絮凝剂对高岭土悬液、土壤悬液和细活性炭粉末悬液和电瓷厂污水均有较好的絮凝作用。     

微生物絮凝剂产生菌的筛选及培养研究

从活性污泥中筛选出3株能产生高效絮凝活性的微生物,对其中1株TJ3进行其最佳培养条件及废水絮凝实验研究。结果表明TJ3的最佳培养条件为：以葡萄糖、蛋白胨作碳,氮源,初始pH值6～7,温度25～30℃,摇床转速120～160r/min,培养时间72h,可产生高絮凝活性的絮凝剂,对高岭土悬浊液絮凝率达92.4%,同时在最佳培养条件下,该菌所产絮凝剂对多种废水净化效果明显,尤其对净化分散兰染液,酵母菌菌液分离最为突出,絮凝率均在95%以上,具有较高的絮凝性能。     

一株絮凝剂产生菌的筛选鉴定及培养条件

从淤泥中筛选到1株高效微生物絮凝剂产生菌JX18,经过对其形态特征、生理生化特性、以及16SrRNA序列分析初步鉴定为荧光假单胞菌。该菌培养40h进入稳定期,48h时絮凝活性达到最大。进一步研究表明:菌株JX18最适培养基初始pH值7.0,培养温度30°C,摇床转速160r/min,最佳碳源和氮源分别为葡萄糖和蛋白胨。最适培养条件下,所产絮凝剂对高岭土悬浮液的絮凝活性达到90.56%。     

Sphingomonas sp. X20絮凝特性及培养条件研究

目的:对分离筛选获得絮凝剂产生菌Sphingomonas sp.X20絮凝特性及培养条件进行研究.方法:采用单因素和正交实验确定最适产絮凝剂培养条件.结果:研究发现,菌株X20的微生物絮凝剂对高岭土悬液具有良好的絮凝效果,在温度为37℃、培养基初始pH7.0、摇床转速为100r/min条件下培养12h获得的絮凝剂絮凝活性最好,絮凝率达92.8%.正交实验结果表明,菌株Sphingomonas sp.X20产絮凝剂最佳培养基的组成:淀粉15g/L,NH4Cl 1.Og/L,KH2P04,2g/L,K2HP04 5g/L,NaC1 O.lg/L,MgS04·7H2O 0.2g/L,pH7.0.结论:菌株X20是一株高效的产絮凝剂菌,具有很好的应用前景.     

一株生物絮凝剂产生菌的筛选及絮凝活性研究

从活性污泥中筛选到一株产絮凝剂的菌株WJ-100。该菌株产絮凝剂的适宜pH塑6．5。适宜温度为25℃～40℃,摇床速度为80～240r／min;Na^＋、K^＋、Ca^2＋、Fe^3＋均有较好的促絮凝作用,Ca^2＋尤为显著;WJ-100以多种糖类为良好碳源,絮凝率达99.2％、99．8％甚至100％;该菌株在高岭土悬液pH2．0～10．0范围均有较好的絮凝效果。WJ-100在较大的pH、温度、碳源、摇床速度、搅拌速度等范围内均具有很高的絮凝活性,显示出重要的研究和应用价值。     

微生物絮凝剂的菌种培养及絮凝活性研究

从活性污泥中分离出具有絮凝活性的菌种作为微生物絮凝剂,通过将其对高岭土悬浊液进行絮凝活性测定,得出了适于该菌产絮凝剂的最佳培养基为：以浓度为1．5％的葡萄糖作为碳源,以NH4Q0．06％ 酵母膏0．04％相组合为氮源,以浓度0．3％的KH2PO4作为无机营养物质,其它培养条件pH值为7～8,温度为30℃,摇床转速为180r／min。并将培养好的菌液用于多种废水净化,试验结果表明：其絮凝率与去除率均达90％以上,具有较好的净化效果。     

产微生物絮凝剂的菌株筛选、鉴定及发酵条件优化

微生物絮凝剂是一类新型的水处理制剂,因其具有高效、无毒、易降解等特点,已成为水处理剂开发的研究热点。为了扩大微生物絮凝剂的种类,本研究从活性污泥和土壤样品中筛选絮凝剂产生菌,最终获得一株具有絮凝活性的菌株,通过16S rDNA鉴定,该菌株为蒙氏假单胞菌(Pseudomonas monteilii),因此命名为P. monteilii YR-1。利用单因素实验和正交试验对该菌株的发酵条件进行了优化,其最佳碳、氮源分别为25 g/L葡萄糖和2 g/L复合氮源(酵母浸粉:尿素:硫酸铵=5:5:2),培养基初始pH值为10,培养温度为28℃,发酵时间为72 h。在最佳培养条件下,其发酵液的絮凝率从37.68%提高到63.52%。这是关于蒙氏假单胞菌产絮凝剂的首次报道。     

Recent advances in the study of Kaposi's sarcoma-associated herpesvirus replication and pathogenesis

It has now been over twenty years since a novel herpesviral genome was identified in Kaposi's sarcoma biopsies. Since then, the cumulative research effort by molecular biologists, virologists, clinicians, and epidemiologists alike has led to the extensive characterization of this tumor virus, Kaposi's sarcoma-associated herpesvirus(KSHV; also known as human herpesvirus 8(HHV-8)), and its associated diseases. Here we review the current knowledge of KSHV biology and pathogenesis, with a particular emphasis on new and exciting advances in the field of epigenetics. We also discuss the development and practicality of various cell culture and animal model systems to study KSHV replication and pathogenesis.     

The structure, reproduction and taxonomy of Vidalia and Osmundaria (Rhodophyta, Rhodomelaceae)

Comprises species occurring mostly in subtidal habitats in tropical, subtropical and warm-temperate areas of the world. An analysis of the type species, V. spiralis (Sonder) Lamouroux ex J. Agardh, a species from Australia, establishes basic characters for distinguishing species in the genus. These characters are (1) branching patterns of thalli, (2) flat blades that may be spiralled on their axis, (3) width of the blade, (4) primary or secondary derivation of sterile and fertile branchlets and (5) position of sterile and fertile branchlets on the thalli. Application of the latter two characters provides an important basic method for separation of species into three major groups. Osmundaria , a genus known only in southern Australia, was studied in relation to Vidalia , and its separation from the Vidalia assemblage is not accepted. Species of Vidalia therefore are transferred to the older genus name, Osmundaria. Two new species, Osmundaria papenfussii and Osmundaria oliveae are described from Natal. Confusion in the usage of the epithet, Vidalia fimbriala Brown ex Turner has been clarified, and Vidalia gregaria Falkenberg, described as an epiphyte on Osmundaria pro/ifera Lamouroux, is revealed to be young branches of the host, Osmundaria prolifera.     

New or rare chromosome counts in Artemisia L. (Asteraceae, Anthemideae) and related genera from Kazakhstan

Fifteen chromosome counts of six Artemisia taxa and one species of each of the genera Brachanthemum, Hippolytia, Kaschgaria, Lepidolopsis and Turaniphytum are reported from Kazakhstan. Three of them are new reports, two are not consistent with previous counts and the remainder are confirmations of very scarce (one to four) earlier records. All the populations studied have the same basic chromosome number, x = 9, with ploidy levels ranging from 2x to 6x. Some correlations between ploidy level, morphological characters and distribution are noted.     

肝癌中HBV和HCV基因和抗原的分布及意义

采用原位分子杂交方法检测HCV RNA及HBV X基因;采用免疫组织化学方法研究HCV核心抗原,非结构区C33c抗原及HBxAg在肝细胞肝癌中的定位及分布.结果表明(1)HCV RNA、HBV X基因在肝细胞肝癌组织检出率分别为40%(55/136)和82%(112/136).HCV RNA定位于癌细胞的胞浆内,阳性细胞呈散在、灶状及弥漫分布三种形式;HBV X基因在肝癌细胞中的分布呈胞浆型、核型及核浆型,阳性细胞也呈上述三种分布形式;(2)HCV C33c抗原、核心抗原在肝细胞肝癌中的阳性率为81%(133/164)及86%(141/164).C33c抗原定位于癌细胞及肝细胞的胞浆内;核心抗原既定位于癌细胞核中,又可定位于胞浆中.C33c抗原阳性细胞以灶状分布为主;而核心抗原阳性细     

Selection with two alleles and complete dominance

For a plant selection model with frequency-independent viabilities, fertilities and selfing rates, it is shown that apart from global fixation, for certain parameter combinations a protected polymorphism and facultative fixation (either allele may become fixed according to initial frequencies) may both occur. Facultative fixation requires different selling rates for the dominant and recessive type. Protection of the polymorphism requires resource allocation for male and female function. In this connection the problem of purely genetically caused population extinction is discussed.
For general frequency dependence and regular segregation, the chances for establishment of a completely recessive gene are compared to those of a completely dominant gene. It is proven that the process of establishment of the recessive gene, despite a fitness advantage, may be considerably endangered by drift effects if random mating prevails. The recessive gene may reach the same effectivity in establishment as a dominant gene, only if the recessive homozygote mates exclusively with its own type during the period of establishment.