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1.
The microcirculation is a complex and integrated system, transporting oxygen and nutrients to the cells. The key component of this system is the endothelium, contributing to the local balance between pro and anti-inflammatory mediators, hemostatic balance, as well as vascular permeability and cell proliferation. A constant shear stress maintains vascular endothelium homeostasis while perturbed shear stress leads to changes in secretion of vasodilator and vasoconstrictor agents. Increased oxidative stress is a major pathogenetic mechanism of endothelial dysfunction by decreasing NO bioavailability, promoting inflammation and participating in activation of intracellular signals cascade, so influencing ion channels activation, signal transduction pathways, cytoskeleton remodelling, intercellular communication and ultimately gene expression. Targeting the microvascular inflammation and oxidative stress is a fascinating approach for novel therapies in order to decrease morbidity and mortality of chronic and acute diseases. 相似文献
2.
The microcirculation is a complex and integrated system, transporting oxygen and nutrients to the cells. The key component of this system is the endothelium, contributing to the local balance between pro and anti-inflammatory mediators, hemostatic balance, as well as vascular permeability and cell proliferation. A constant shear stress maintains vascular endothelium homeostasis while perturbed shear stress leads to changes in secretion of vasodilator and vasoconstrictor agents. Increased oxidative stress is a major pathogenetic mechanism of endothelial dysfunction by decreasing NO bioavailability, promoting inflammation and participating in activation of intracellular signals cascade, so influencing ion channels activation, signal transduction pathways, cytoskeleton remodelling, intercellular communication and ultimately gene expression. Targeting the microvascular inflammation and oxidative stress is a fascinating approach for novel therapies in order to decrease morbidity and mortality of chronic and acute diseases. 相似文献
3.
Andréia Machado Cardoso Caroline Curry Martins Fernando da Silva Fiorin Roberta Schmatz Fátima Husein Abdalla Jessié Gutierres Daniela Zanini Amanda Maino Fiorenza Naiara Stefanello Jonas Daci da Silva Serres Fabiano Carvalho Verônica Paiva Castro Cinthia Melazzo Mazzanti Luiz Fernando Freire Royes Adriane Belló‐Klein Jeferson Ferraz Goularte Vera Maria Morsch Margarete Dulce Bagatini Maria Rosa Chitolina Schetinger 《Cell biochemistry and function》2013,31(2):136-151
The present study investigated the effects of a 6‐week swimming training on blood pressure, nitric oxide (NO) levels and oxidative stress parameters such as protein and lipid oxidation, antioxidant enzyme activity and endogenous non‐enzymatic antioxidant content in kidney and circulating fluids, as well as on serum biochemical parameters (cholesterol, triglycerides, urea and creatinine) from Nω‐nitro‐L‐arginine methyl ester hydrochloride (L‐NAME)‐induced hypertension treated rats. Animals were divided into four groups (n = 10): Control, Exercise, L‐NAME and Exercise L‐NAME. Results showed that exercise prevented a decrease in NO levels in hypertensive rats (P < 0·05). An increase in protein and lipid oxidation observed in the L‐NAME‐treated group was reverted by physical training in serum from the Exercise L‐NAME group (P < 0·05). A decrease in the catalase (CAT) and superoxide dismutase (SOD) activities in the L‐NAME group was observed when compared with normotensive groups (P < 0·05). In kidney, exercise significantly augmented the CAT and SOD activities in the Exercise L‐NAME group when compared with the L‐NAME group (P < 0·05). There was a decrease in the non‐protein thiols (NPSH) levels in the L‐NAME‐treated group when compared with the normotensive groups (P < 0·05). In the Exercise L‐NAME group, there was an increase in NPSH levels when compared with the L‐NAME group (P < 0·05). The elevation in serum cholesterol, triglycerides, urea and creatinine levels observed in the L‐NAME group were reverted to levels close to normal by exercise in the Exercise L‐NAME group (P < 0·05). Exercise training had hypotensive effect, reducing blood pressure in the Exercise L‐NAME group (P < 0·05). These findings suggest that physical training could have a protector effect against oxidative damage and renal injury caused by hypertension. Copyright © 2012 John Wiley & Sons, Ltd. 相似文献
4.
Kyoung Ah Kang Sungwook Chae Kyung Hwa Lee Rui Zhang Myung Sun Jung Ho Jin You Jin Sook Kim Jin Won Hyun 《Biotechnology and Bioprocess Engineering》2005,10(6):556-563
Homogentisic acid was found to scavenge intracellular reactive oxygen species (ROS), and 1,1-diphenyl-2-picrylhydrazyl (DPPH)
radicals and thus prevented lipid peroxidation in human fibroblast (WI 38) cells. The radical scavenging activity of homogentisic
acid was found to protect WI 38 cells against hydrogen peroxide (H2O2) induced oxidative stress, via the activation of extracellular signal regulated kinase (ERK) protein. Homogentisic acid increased
the activity of catalase. Hence, from the present study, it is suggested that homogentisic acid protects WI 38 cells against
H2O2 damage by enhancing the intracellular antioxidative activity. 相似文献
5.
Natalie E. de Picciotto Lindsey B. Gano Lawrence C. Johnson Christopher R. Martens Amy L. Sindler Kathryn F. Mills Shin‐ichiro Imai Douglas R. Seals 《Aging cell》2016,15(3):522-530
We tested the hypothesis that supplementation of nicotinamide mononucleotide (NMN), a key NAD+ intermediate, increases arterial SIRT1 activity and reverses age‐associated arterial dysfunction and oxidative stress. Old control mice (OC) had impaired carotid artery endothelium‐dependent dilation (EDD) (60 ± 5% vs. 84 ± 2%), a measure of endothelial function, and nitric oxide (NO)‐mediated EDD (37 ± 4% vs. 66 ± 6%), compared with young mice (YC). This age‐associated impairment in EDD was restored in OC by the superoxide () scavenger TEMPOL (82 ± 7%). OC also had increased aortic pulse wave velocity (aPWV, 464 ± 31 cm s?1 vs. 337 ± 3 cm s?1) and elastic modulus (EM, 6407 ± 876 kPa vs. 3119 ± 471 kPa), measures of large elastic artery stiffness, compared with YC. OC had greater aortic production (2.0 ± 0.1 vs. 1.0 ± 0.1 AU), nitrotyrosine abundance (a marker of oxidative stress), and collagen‐I, and reduced elastin and vascular SIRT1 activity, measured by the acetylation status of the p65 subunit of NFκB, compared with YC. Supplementation with NMN in old mice restored EDD (86 ± 2%) and NO‐mediated EDD (61 ± 5%), reduced aPWV (359 ± 14 cm s?1) and EM (3694 ± 315 kPa), normalized production (0.9 ± 0.1 AU), decreased nitrotyrosine, reversed collagen‐I, increased elastin, and restored vascular SIRT1 activity. Acute NMN incubation in isolated aortas increased NAD+ threefold and manganese superoxide dismutase (MnSOD) by 50%. NMN supplementation may represent a novel therapy to restore SIRT1 activity and reverse age‐related arterial dysfunction by decreasing oxidative stress. 相似文献
6.
Seaman C Moritz J Falkenstein E Van Dyke K Casotti G Klandorf H 《Comparative biochemistry and physiology. Part A, Molecular & integrative physiology》2008,151(4):670-675
The objective of these studies was to determine whether inosine, a precursor of the antioxidant uric acid, can ameliorate hemin-induced oxidative stress. Dietary inclusion of inosine was begun either before or after hemin-induced oxidative stress. Broilers (4 weeks) were divided into four treatment groups (Control, Hemin, Inosine, Hemin/Inosine). Throughout the study control birds (n = 10) were injected daily with a buffer solution, while hemin birds (n = 10) were injected daily (i.p.) with a 20 mg/kg body weight hemin buffer solution. Leukocyte oxidative activity (LOA) and concentrations of plasma uric acid (PUA) were measured. Results from the first study showed that hemin birds had increased levels of LOA (P = 0.0333) and lower PUA (P = 0.1174). On day 10, control and hemin birds were subdivided into inosine birds (n = 5) and hemin/inosine birds (n = 5). These birds were given 0.6 M/kg of feed/day of dry inosine. Plasma concentrations of uric acid and LOA were then measured on day 15. Results showed that inosine raised concentrations of PUA (P = 0.0001) and lowered LOA (P = 0.0044) as induced by hemin. In the second study pretreatment of broilers with hemin prevented the increase in LOA induced by hemin (P = 0.0001). These results show that modulating the concentrations of uric acid can markedly affect oxidative stress. 相似文献
7.
Camila C. Pinto Kamila C. Silva Subrata K. Biswas Natássia Martins José B. Lopes De Faria 《Free radical research》2013,47(10):1151-1158
The present study was undertaken to investigate the redox status in the retina of an experimental model that combines hypertension and diabetes. Spontaneously hypertensive rats (SHR) and their control Wystar Kyoto (WKY) rats were rendered diabetic and, after 20 days, the rats were sacrificed and the retinas collected. The superoxide production was higher in diabetic than in control WKY (p<0.03) and SHR rats showed elevated superoxide production compared with WKY groups (p<0.009). The glutathione antioxidant system was diminished only in diabetic SHR (p<0.04). Tirosyne nitration was higher in diabetic WKY and control SHR compared with control WKY (p<0.03), and further increment was observed in diabetic SHR (p<0.02). The DNA damage estimated by immunohystochemistry for 8-OHdG was higher in control SHR than in WKY, mainly in diabetic SHR (p<0.0001). Hypertension aggravates oxidative-induced cytotoxicity in diabetic retina due to increasing of superoxide production and impairment of antioxidative system. 相似文献
8.
The short- and long-term pro-oxidant effect of protoporphyrin IX (PROTO) administration to mice was studied in liver. A peak of liver porphyrin accumulation was found 2 h after the injection of PROTO (3.5 mg/kg, i.p.); then the amount of porphyrins diminished due to biliar excretion. After several doses of PROTO (1 dose every 24 h up to 5 doses) a sustained enhancement of liver porphyrins was observed. The activity of δ-amino-levulinic acid synthetase was induced 70–90% over the control values 4 h after the first injection of PROTO and stayed at these high levels throughout the period of the assay. Administration of PROTO induced rapid liver damage, involving lipid peroxidation. Hepatic GSH content was increased 2 h after the first injection of PROTO, but then decreased below the control values which were maintained after several doses of porphyrin. After a single dose of PROTO, Cu-Zn superoxide dismutase (SOD) was rapidly induced, suggesting that superoxide radicals had been generated. Increased levels of hydrogen peroxide coming from the reaction catalyzed by SOD and lipid peroxides as a consequence of membrane peroxidation, induced the activity of catalase and glutathione peroxidase (GPx), while decreased GSH levels induced glutathione reductase (GRed) activity. However after 5 doses of PROTO, the activity of SOD was reduced reaching control values. GPx and catalase activities slowly went down, while GRed continued increasing as long as the levels of GSH were kept very low. TBARS values, although lower than those observed after a single dose of PROTO, remained above control values; Glutathione S-transferase activity was instead greatly diminished, indicating sustained liver damage.Our findings would indicate that accumulation of PROTO in liver induces oxidative stress, leading to rapid increase in the activity of the antioxidant enzymes to avoid or revert liver damage. However, constant accumulation of porphyrins provokes a liver damage so severe that the antioxidant system is compromised. 相似文献
9.
Subhalaxmi Mohapatra 《Archives Of Phytopathology And Plant Protection》2017,50(1-2):84-96
Trichoderma viride (Pers.) pre-inoculated wheat seedlings infected with Fusarium oxysporum Schlecht. (co-stressed) did not show wilting symptoms compared to Fusarium infected seedlings. Antagonistic activity of T. viride could be demonstrated against Fusarium infection by dual culture experiment. After seven days post infection, morphological and physiological parameters such as, root and shoot length, fresh and dry weight, relative water content, total soluble protein, total chlorophyll and carotenoid contents were observed to be increased in co-stressed compared to Fusarium infected seedlings. Accumulation of hydrogen peroxide was enhanced in Fusarium infected tissues compared to co-stressed. Trichoderma mediated activation of antioxidant enzymes such as, catalase, guaiacol peroxidase, ascorbate peroxidase, superoxide dismutase in co-stressed seedlings indicated their involvement in enhanced resistance against Fusarium infection, which is suggestive of playing crucial role in mitigating cellular toxicity developed due to excess H2O2. Thus, Trichoderma pre-inoculation protected wheat against Fusarium infection by stabilising oxidative stress. 相似文献
10.
目的:研究肾上腺髓质素2(ADM2)拮抗血管紧张素Ⅱ(AngⅡ)发挥舒张血管的作用及机制。方法:将18只180~200 g雄性SD大鼠随机分为3组(n=6):对照组、AngⅡ(150 ng/(kg·min))组和AngⅡ(150 ng/(kg·min))+ADM2(500 ng/(kg·h))组,采用皮下埋植微量渗透泵的方法给药。2周后颈动脉插管法测量大鼠血压,测定血浆一氧化氮(NO)含量和内皮型一氧化氮合酶(eNOS)活性。DHE染色法检测大鼠动脉壁活性氧产生。制备大鼠离体血管环,观察ADM2的舒血管作用。培养人脐静脉内皮细胞系EA.hy 926,用DCFH-DA荧光探针检测AngⅡ和ADM2对血管内皮细胞活性氧释放的影响。结果:与AngⅡ组相比,ADM2显著降低了大鼠血压,血浆中eNOS活性提高、NO含量增加,血管壁活性氧产生减少。ADM2呈浓度依赖性和内皮依赖性舒张血管环,并明显抑制了AngⅡ引起的血管内皮细胞活性氧产生。结论:ADM2可能通过拮抗AngⅡ诱导的血管内皮氧化应激效应,改善内皮功能,发挥舒张血管、降低血压的作用。 相似文献
11.
Singal Pawan K. Farahmand Firoozeh Hill Michael F. 《Molecular and cellular biochemistry》2004,260(1):21-29
Although right heart failure (RHF) contributes to 20% of all cardiovascular complications, most of the information available on RHF in general is based on the experiences with left heart failure. This study on RHF investigates changes in antioxidants and oxidative stress which are suggested to play a role in the transition from hypertrophy to failure. RHF subsequent to pulmonary hypertension was produced in rats by a single injection of monocrotaline (MCT, 60 mg/kg, i.p.). Based on hemodynamic, clinical and histopathologic observations, the animals were grouped in three functional stages at 1-, 2- and 6-week post-injection periods. In the 1-week group, RV pressure overload and hypertrophy, and a mild increase in antioxidant enzymes was seen. In the 2-week group, compensated HF, a significant increase in antioxidant enzymes, an increase in septal (IVS) wall thickness and leftward displacement of IVS without change in LV free wall were seen. In the 6-week group, lung and liver congestion, RVF and dilation, a decrease in antioxidant enzyme activities, increase in lipid peroxidation and severe bulging of the IVS into the left ventricle were seen. These changes in the hemodynamic, biochemical and histopathologic characteristics suggest that in early stages of MCT-induced pulmonary hypertension at 1 and 2 weeks, RV hypertrophy was accompanied by sustained hemodynamic function and an increase in antioxidant reserve. In the later stage at 6 weeks, clinical RHF was associated with abnormalities of the right heart systolic and diastolic function along with a decrease in antioxidant reserve. These biphasic changes in RV antioxidant enzymes, i.e. an increase during hypertrophy and a decrease in failure may suggest a role of oxidative stress in the pathogenesis of right ventricular dysfunction. 相似文献
12.
Rosiglitazone via PPARγ‐dependent suppression of oxidative stress attenuates endothelial dysfunction in rats fed homocysteine thiolactone
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Xu‐Hong Yang Peng Li Ya‐Ling Yin Jiang‐Hua Tu Wen Dai Li‐Ying Liu Shuang‐Xi Wang 《Journal of cellular and molecular medicine》2015,19(4):826-835
To explore whether rosiglitazone (RSG), a selective peroxisome proliferator‐activated receptor γ (PPARγ) agonist, exerts beneficial effects on endothelial dysfunction induced by homocysteine thiolactone (HTL) and to investigate the potential mechanisms. Incubation of cultured human umbilical vein endothelial cells with HTL (1 mM) for 24 hrs significantly reduced cell viabilities assayed by 3‐(4,5‐dimethyl‐2‐thiazolyl)‐2,5‐diphenyl‐2‐H‐tetrazolium bromide, as well as enhanced productions of reactive oxygen species, activation of nuclear factor kappa B, and increased intercellular cell adhesion molecule‐1 secretion. Pre‐treatment of cells with RSG (0.001–0.1 mM), pyrollidine dithiocarbamate (PDTC, 0.1 mM) or apocynin (0.1 mM) for 1 hr reversed these effects induced by HTL. Furthermore, co‐incubation with GW9662 (0.01 mM) abolished the protective effects of RSG on HTL‐treated cells. In ex vivo experiments, exposure of isolated aortic rings from. rats to HTL (1 mM) for 1 hr dramatically impaired acetylcholine‐induced endothelium‐dependent relaxation, reduced release of nitric oxide and activity of superoxide dismutase, and increased malondialdehyde content in aortic tissues. Preincubation of aortic rings with RSG (0.1, 0.3, 1 mM), PDTC or apocynin normalized the disorders induced by HTL. In vivo analysis indicated that administration of RSG (20 mg/kg/d) remarkably suppressed oxidative stress and prevented endothelial dysfunction in rats fed HTL (50 mg/kg/d) for 8 weeks. RSG improves endothelial functions in rats fed HTL, which is related to PPARγ‐dependent suppression of oxidative stress. 相似文献
13.
The role of oxidative stress in immune cell toxicity caused by the pesticides lindane, malathion and permethrin was investigated
in thymic cells from C57BL/6 mice. Thymocytes treated with any of these pesticides (concentrations ranging between 50–150
μM) were found to generate both superoxide (•O2
−) and H2O2. The production of •O2
− was detected with hydroethidine-ethidium bromide assay. H2O2 production was monitored with a flow cytometric fluorescent (DCFH-DA) assay. All three pesticides stimulated •O2
− release after 5 min exposure. Lindane and permethrin, but not malathion, continued to have significant (p ≤ 0.05) effects on •O2
− generation following 15 min of exposure. The lindane + malathion mixture was found to cause more-than-additive increase in
•O2
− production compared to individual pesticide treatments (at both 5 and 15 min). However, the effect of the lindane + permethrin
mixture was not significantly different than individual components of this mixture. The effects of these pesticides on levels
of antioxidant enzymes were also investigated, and only mixtures were found to have significant (p ≤ 0.05) effects. Thus, lindane + malathion and lindane + permethrin mixtures increased total superoxide dismutase (SOD) specific
activity, had no effect on catalase levels and inhibited GSH-peroxidase and GSH-reductase specific activities. Although the
results of these studies do not explain the mechanism of action of these pesticides on the generation of •O2
− and H2O2, it is worthy of note that mixtures of these chemicals have oxidative responses greater than those of single chemicals.
An erratum to this article can be found at 相似文献
14.
Pannexin hemichannels: A novel promising therapy target for oxidative stress related diseases
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15.
Zeliha Selamoglu Talas 《Cell biochemistry and function》2014,32(2):150-154
The inhibition in the synthesis or bioavailability of nitric oxide (NO) has an important role in progress of hypertension. The blocking of nitric oxide synthase activity may cause vasoconstriction with the formation of reactive oxygen species (ROS). Propolis is a resinous substance collected by honey bees from various plants. Propolis has biological and pharmacological properties. The aim of this study was to examine the effect of propolis on catalase (CAT) activity, malondialdehyde (MDA) and NO levels in the testis tissues of hypertensive rats by Nω‐nitro‐l ‐arginine methyl ester (l ‐NAME). Rats have received nitric oxide synthase inhibitor (l ‐NAME, 40 mg kg?1, intraperitoneally) for 15 days to produce hypertension and propolis (200 mg kg?1, by gavage) during the last 5 days. MDA level in l ‐NAME‐treated group significantly increased compared with control group (P < 0.01). MDA level of l ‐NAME + propolis‐treated rats significantly reduced (P < 0.01) compared with l ‐NAME‐treated group. CAT activity and NO level significantly reduced (P < 0.01) in l ‐NAME group compared with control group. There were no statistically significant increases in the CAT activity and NO level of the l ‐NAME + propolis group compared with the l ‐NAME‐treated group (P > 0.01). These results suggest that propolis changes CAT activity, NO and MDA levels in testis of l ‐NAME‐treated animals, and so it may modulate the antioxidant system. Copyright © 2013 John Wiley & Sons, Ltd. 相似文献
16.
We investigated the ability of cadmium and mercury ions to cause endothelial dysfunction in bovine pulmonary artery endothelial
cell monolayers. Exposure of monolayers for 48 h to metal concentrations greater than 3–5 μM produced profound cytotoxicity
(increased lactate dehydrogenase leakage), a permeability barrier failure, depletion of glutathione and ATP and almost complete
inhibition of the activity of key thiol enzymes, glucose-6-phosphate dehydrogenase (G6PDH) and glyceraldehyde-3-phosphate
dehydrogenase (GAPDH). In contrast, metal concentrations less than 1–2 μM induced increases in glutathione and thiol-enzyme
activities with minimal changes in LDH leakage, barrier function and ATP content. At shorter incubation times (24 h or less),
high concentrations of cadmium caused glutathione induction rather than depletion. Thus, oxidative stress and cytotoxicity
induced by lower concentrations of the metal ions stimulate compensatory responses, including increased synthesis of glutathione,
which presumably preserved the activity of key thiol enzymes, however these responses were not sustainable at higher metal
ion concentrations. We conclude, while high concentrations of heavy metals are cytotoxic, lower concentration induce a compensatory
protective response, which may explain threshold effects in metal-ion toxicity. 相似文献
17.
Xingzhi Jing Ting Du Tao Li Xiaoxia Yang Guodong Wang Xiaoyang Liu Zhensong Jiang Xingang Cui 《Journal of cellular and molecular medicine》2021,25(12):5671-5680
Iron overload is common in elderly people which is implicated in the disease progression of osteoarthritis (OA), however, how iron homeostasis is regulated during the onset and progression of OA and how it contributes to the pathological transition of articular chondrocytes remain unknown. In the present study, we developed an in vitro approach to investigate the roles of iron homeostasis and iron overload mediated oxidative stress in chondrocytes under an inflammatory environment. We found that pro-inflammatory cytokines could disrupt chondrocytes iron homeostasis via upregulating iron influx transporter TfR1 and downregulating iron efflux transporter FPN, thus leading to chondrocytes iron overload. Iron overload would promote the expression of chondrocytes catabolic markers, MMP3 and MMP13 expression. In addition, we found that oxidative stress and mitochondrial dysfunction played important roles in iron overload-induced cartilage degeneration, reducing iron concentration using iron chelator or antioxidant drugs could inhibit iron overload-induced OA-related catabolic markers and mitochondrial dysfunction. Our results suggest that pro-inflammatory cytokines could disrupt chondrocytes iron homeostasis and promote iron influx, iron overload-induced oxidative stress and mitochondrial dysfunction play important roles in iron overload-induced cartilage degeneration. 相似文献
18.
Elo F. Yamada Franciane Bobinski Daniel F. Martins Juliete Palandi Vanderlei Folmer Morgana D. da Silva 《Journal of biophotonics》2020,13(1)
Knee osteoarthritis (OA) is a chronic disease that causes pain and gradual degeneration of the articular cartilage. In this study, MIA‐induced OA knee model was used in rats to test the effects of the photobiomodulation therapy (PBM). We analyzed the inflammatory process (pain and cytokine levels), and its influence on the oxidative stress and antioxidant capacity. Knee OA was induced by monosodium iodoacetate (MIA) intra‐articular injection (1.5 mg/50 μL) and the rats were treated with eight sessions of PBM 3 days/week (904 nm, 6 or 18 J/cm2). For each animal, mechanical and cold hyperalgesia and spontaneous pain were evaluated; biological analyses were performed in blood serum, intra‐articular lavage, knee structures, spinal cord and brainstem. Cytokine assays were performed in knee, spinal cord and brainstem samples. The effects of the 18 J/cm2 dose of PBM were promising in reducing pain and neutrophil activity in knee samples, together with reducing oxidative stress damage in blood serum and spinal cord samples. PBM improved the antioxidant capacity in blood serum and brainstem, and decreased the knee pro‐inflammatory cytokine levels. Our study demonstrated that PBM decreased oxidative damage, inflammation and pain. Therefore, this therapy could be an important tool in the treatment of knee OA. 相似文献
19.
Eugène H. J. M. Jansen Piet K. Beekhof Dale Viezeliene Vladimira Muzakova Jiri Skalicky 《Free radical research》2017,51(11-12):970-977
The storage time and storage temperature might affect stability of oxidative stress biomarkers, therefore, they have to be analyzed after long-term storage of serum samples. The stability of three biomarkers reflecting oxidative stress: reactive oxygen metabolites (ROM) for hydroperoxides, total thiol levels (TTL) for the redox status and biological antioxidant potency (BAP) for the antioxidant status, was investigated at several time points during 60 months of storage at ?20 and ?80?°C. Biomarkers ROM and BAP showed a very good stability during storage for 60 months at both temperatures. In addition, the correlation of the data after 60 months of storage compared with the starting data was very good with correlation coefficients >0.9. The TTL assay showed good results in serum samples stored at ?80?°C, but not in samples stored at ?20?°C. Serum samples for analysis of the set of oxidative stress biomarkers ROM, BAP and TTL can be stored up to 60 months at ?80?°C. ROM and BAP can also be stored at ?20?°C during this period. The present results are very important for the biomarker-related epidemiological studies that make use of biobanks with samples stored for many years and for new project planning, including sample storage conditions. 相似文献
20.
Demirci S Sekeroğlu MR Noyan T Köçeroğlu R Soyoral YU Dülger H Erkoç R 《Cell biochemistry and function》2011,29(3):249-254
Increased oxidative stress is a well-known phenomenon in dialysis patients. However, the contribution of hypertension to the oxidative stress in peritoneal dialysis patients has not yet been assessed. The present study aimed to investigate if hypertension had an additional effect on oxidative stress in peritoneal dialysis patients. A total of 50 patients treated with peritoneal dialysis were divided into two groups: The patients with mean of last three blood pressure results as 135/90 mmHg and above were considered hypertensive, the patients with lower blood pressure were considered normotensive. The control group included 25 healthy individuals. Serum malondialdehyde (MDA), advanced oxidation protein product (AOPP), myeloperoxidase (MPO), catalase (CAT) and glutathione peroxidase (GSH-Px) levels were measured in all groups. MDA level, an indicator of lipid peroxidation, was significantly higher in the hypertensive group compared to the control group, while the increase in the normotensive group was not significant. However, the difference between the hypertensive and normotensive groups was significant. The levels of AOPP, an indicator of protein oxidation level, and MPO, an indicator of neutrophil activation, were not different between the groups, while the activities of antioxidant CAT and GSH-Px decreased in both normotensive and hypertensive groups compared to the control group, and there was no significant difference between the patient groups. This study shows that both normotensive and hypertensive peritoneal dialysis patients have increased-oxidative stress and decreased antioxidant levels and hypertension might have an additional effect on oxidative stress by increasing MDA level in peritoneal dialysis patients. 相似文献