共查询到20条相似文献,搜索用时 15 毫秒
1.
Rusaouën-Innocent M 《Tissue & cell》1990,22(4):449-462
Optical microscope and ultrastructural autoradiography have been used to localise the sites of collagen secretion in the gland zones, and track its cellular synthesis and mode of cellular secretion. Optical microscope observation has been done after (3)H proline injection into the gland. Untrastructural study has been achieved after applying the incubation method. Optical microscope study has demonstrated that all the gland zones were involved in the secretion of the collageneous protein, though some of them (the D zone and lamellae) secrete more collagen than the others. The ultrastructural study demonstrates that the forms of secretion are numerous: various-looking grains, whose elaboration follows the path of vertebrate collagen produced by fibroblasts, or grains whose only source is rER. The protein periodicity which is observed in the mature capsule (37 nm) is acquired only outside the cells, in the tubular or glandular lumen. It is possible that two types of collagen are secreted by the gland, the first forming the major part of the capsule wall, the other contributing to the plugs sealing the slits of dehiscence. 相似文献
2.
Weronika Rupik 《Zoology (Jena, Germany)》2012,115(6):389-397
The mechanism of follicular lumen differentiation during thyroid gland morphogenesis in vertebrate classes is still unclear and the current knowledge regarding the origin and the mechanism of follicular lumen formation during thyroid differentiation in reptiles is especially poor. The present study reports on an ultrastructural investigation of thyroid follicle formation and follicular lumen differentiation in grass snake (Natrix natrix L.) embryos. The results of this study show that the earliest morphogenesis of the presumptive thyroid follicles in grass snake embryos appears to be similar to that described in embryos of other vertebrate classes; however, differences appeared during the later stages of its differentiation when the follicular lumen was formed. The follicular lumen in grass snake embryos was differentiated by cavitation; during thyroid follicle formation, a population of centrally located cells was cleared through apoptosis to form the lumen. This manner of follicular lumen differentiation indicates that it has an extracellular origin. It cannot be excluded that other types of programmed cell death also occur during follicular lumen formation in this snake species. 相似文献
3.
G Morel P Dubois F Gossard 《Comptes rendus de l'Académie des sciences. Série III, Sciences de la vie》1986,302(13):479-484
Ultrastructural detection of the messenger RNA coding for growth hormone in rat pituitary gland could be obtained by association of in situ hybridization and cryoultramicrotomy. Messenger RNAs were localized in the anterior pituitary gland. Silver grain densities observed in autoradiograms after in situ hybridization were dependent to incubation period and to fixation. It was necessary to determine a compromise between ultrastructural aspect and silver grain densities. Messenger RNAs were detected in somatotropic cells, identified by ultrastructural characteristics, in both the nucleus (euchromatin and nuclear membrane) and cytoplasm, in vicinity to endoplasmic reticulum. 相似文献
4.
Baccari GC Chieffi G Di Matteo L Dafnis D De Rienzo G Minucci S 《Journal of morphology》2000,244(2):137-142
The Harderian gland of the gecko, Tarentola mauritanica, was studied at the histological, histochemical, and ultrastructural levels. It is a nonlobate compound acinar gland surrounded by a thin capsule of connective tissue. Numerous connective tissue-type mast cells, ultrastructurally similar to those described in other higher vertebrates, were identified in the interstitial tissue between the acini. Pyramidal or columnar-shaped secretory glandular cells were observed in the acini. In the glandular cells, two types of structures could be distinguished on the basis of their high or low electron density. Lipid droplets were found in the cytoplasm of the Harderian gland of both sexes. Histochemical tests showed that the Harderian gland of the gecko is a seromucous gland. The secretion is essentially merocrine, although an apocrine type of secretion is sometimes observed. 相似文献
5.
Rupik W 《Zoology (Jena, Germany)》2011,114(5):284-297
The differentiation of the thyroid primordium of reptilian species is poorly understood. The present study reports on structural and ultrastructural studies of the developing thyroid gland in embryos of the grass snake Natrix natrix L. At the time of oviposition, the thyroid primordium occupied its final position in the embryos. Throughout developmental stages I-IV, the undifferentiated thyroid primordium contained cellular cords, and the plasma membranes of adjacent cells formed junctional complexes. Subsequently, the first follicular lumens started to form. The follicular lumens were of intracellular origin, as in other vertebrate species, but the mechanism of their formation is as yet unclear. At developmental stages V-VI, the thyroid anlage was composed of small follicles with lumens and cellular cords. Cells of the thyroid primordium divided, and follicles were filled with a granular substance. At developmental stage VI, the cells surrounding the follicular lumen were polarized, the apical cytoplasm contained dark granules and the Golgi complex and the rough endoplasmic reticulum (RER) developed gradually. Resorption of the colloid began at developmental stage VIII. At the end of this stage, the embryonic thyroid gland was surrounded by a definitive capsule. During developmental stages IX-X, the follicular cells contained granules and vesicles of different sizes and electron densities and a well-developed Golgi apparatus and RER. At developmental stage XI, most follicles were outlined by squamous epithelial cells and presented wide lumens filled with a light colloid. The Golgi complex and RER showed changes in their morphology indicating a decrease in the activity of the thyroid gland. At developmental stage XII, the activity of the embryonic thyroid gradually increased, and at the time of hatching, it exhibited the features of a fully active gland. 相似文献
6.
Ultrastructural changes of the hatching gland during electrically induced precocious secretion were compared with those during natural secretion in the medaka, Oryzias latipes. The gland cells are covered by a layer of epithelial cells, which adjoin one another just on the apical center of each gland cell. When the natural as well as the precocious secretion occurred, each gland cell was swollen upward and rounded, and separation of the epithelial joints occurred, giving rise to an exposure of the apical portion of the gland cells. There were marked differences between these two kinds of secretion process in the behavior of the secretory granules prior to secretion and in the mode of discharge of the secretory substances. The changes which occurred during both types of secretion and which, therefore, seemed to be essential to the secretory processes of this gland cell were the swelling up of the gland cells in the initiation of secretion and the reduction of the electron density of the zymogen granules. These secretion-associated ultrastructural changes are discussed in view of the difference in the maturation of the gland cells. 相似文献
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8.
Summary The protothyroid region in the endostyles of four species of tunicates was examined by means of autoradiography and cytochemistry, at both the light and electronmicroscopic levels. To reveal the primary binding site for iodine, autoradiography was carried out on endostylar tissue from animals that had been incubated with high activity 125I- over a short period of time. The specific iodine binding enzyme, a peroxidase, was traced by its reaction with DAB. In accordance with previous findings, the iodinebinding cells proved to be the same as those containing the peroxidase. There were also strong indications of a secondary uptake of iodinated compounds and subsequent release into the body fluid. Together with the ultrastructural features, the data provided strong evidence indicating that these cells constitute a protothyroid region, which partly functions as an endocrine organ, possibly homologous with the vertebrate thyroid gland. Since the number of zones varied between the species, the numeration of the protothyroid region also varied. However, in all the examined endostyles, the protothyroid region was seen to be situated dorsolaterally to the glandular regions of the endostyle concerned with food capture. 相似文献
9.
The digestive gland of Viviparus ater was studied using histochemical and ultrastructural methods. Only one cell type was observed in the tubule epithelium of the gland. The cells are involved in an endocytotic process mediated by clathrin-coated vesicles and in the intracellular digestion of food materials (thus they can be regarded as digestive cells). The different stages of digestion and exocytotic extrusion of residual bodies into the tubule lumen were shown by electron microscopy. Very few, small mucocytes are scattered among the digestive cells. Calcium concretions, glycogen-containing cells and endocrine cells are scattered in the area of connective tissue present among the digestive tubules. 相似文献
10.
In this study, we describe the ultrastructural features of the external nasal gland in two lizards: ruin lizard (Podarcis sicula campestris) and seps (Chalcides chalcides). Two secretory cell types, which differ interspecifically, have been found in the secretory endpieces of the glandular tubules in both species examined. An unusual morphological observation was the presence of paracrystalline structures in the secretory granules of the seromucous cells of the external nasal gland of the seps. These structures may be related to the packaging mechanism of glycoproteins or to their macromolecular structure. They may also reflect segregation of heterogeneous subcomponents within the same secretory granule. The striated cells are located in the distal segment of the glandular tubules, and have the typical ultrastructural features of the cells which in some species of reptiles, but not in these two lizards, are known to be capable of elaborating a hyperosmotic saline solution. 相似文献
11.
Electron-microscopic studies were made on the parathyroid gland of cadmium-treated mice. Most chief cells in treated mice were rich in free ribosomes and secretory granules compared to the control mice. In the parathyroid gland after 3 and 4 weeks of administration of cadmium, interdigitations between adjacent chief cells became more complex than in the control mice. In most chief cells of the parathyroid gland after 2, 3 and 4 weeks of administration of cadmium, the Golgi complexes associated with numerous prosecretory granules were better developed than in the control mice. These ultrastructural appearances suggest that parathyroid gland cellular activity was stimulated in response to cadmium treatment. 相似文献
12.
The microcysts found in two adenohypophyses removed from patients with nonsecreting adenomas of this gland have been studied. In one case the cysts were completely surrounded by neoplastic tissue, whereas in the second they were located within the normal parenchyma. The cysts were surrounded by a basement membrane, and appeared to contain a finely granular weakly electron-dense substance. Their walls were lined by mostly cubic cells with microvilli, joined by junctional complexes. Intracytoplasmic microlumina could be seen in some of these cells. Cells showing cilia with a 9 + 2 fibrillar pattern and cells with mucosecretory differentiation were also present. Furthermore endocrine cells were also found in the cyst-lining epithelium. The ultrastructural aspects of the cyst cells lead to reject the hypothesis that these are derived from folliculostellate cells. Besides, intermediate aspects between cyst cells and tumor cells were never visible. Therefore it seems likely that a relationship between adenoma and cyst cells does not exist and that cyst cells originate from remnants of Rathke's pouch with a ciliate or mucosecretory differentiation. The presence of endocrine cells in the cyst-lining epithelium deserves further study, for it seems to indicate that an endocrine differentiation may occur in the adult, too. To our knowledge this is the first ultrastructural study of these structures in adult human pituitary gland. 相似文献
13.
Light and transmission electron microscopy have been used to study the gastrodermal gland cells of the triclad Dugesia gonocephala s.l. The events involved in the ultrastructural transformation and the secretion process in these cells were followed at four different stages in both fasted and fed animals. During the feeding stage their secretory granules are directly discharged into the intestinal lumen by means of a secretion process of the holocrine type that is described in this paper. It is suggested that such secretions contribute to extracellular digestion and that disintegration of the gland cells is accompanied by a differentiation of neoblasts into new gland cells, reflecting a turnover of gland cells during the triclad digestive stages. 相似文献
14.
G Lascar J Taxi 《Comptes rendus de l'Académie des sciences. Série III, Sciences de la vie》1986,303(6):211-216
Each adrenal gland of the Axolotl consists of a strip lying all along the medio-lateral edge on the ventral surface of the kidney. The gland is composed of interrenal cells (IC) and chromaffin cells (CC). The IC contained a great number of pleomorphic lipid droplets, smooth endoplasmic reticulum and elongated mitochondria with tubulo-vesicular cristae. Two types of CC, always disposed in clusters and exhibiting long cytoplasmic processes were described according to the electron density, size and shape of granules distributed in their cytoplasm; noradrenaline cells (NA) and adrenaline cells (A). The innervation and ultrastructural differences from the adrenal gland of other Anurans were discussed. 相似文献
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16.
Maria Mastrodonato Elena Lepore Miriam Gherardi Sara Zizza Margherita Sciscioli Domenico Ferri 《Invertebrate Biology》2005,124(4):303-309
Abstract. This histochemical and ultrastructural study describes the epidermal gland cells of a tubicolous polychaete, Branchiomma luctuosum . The histochemistry was carried out using standard techniques and FITC-labelled lectins. Four types of secretory cells were identified in two categories: orthochromatic cells (Type 1) and metachromatic cells (Types 2, 3, and 4). The secretory product of the Type-1 orthochromatic cells contains neutral glycoproteins with Galβ1,3GalNAc residues. Metachromatic cells produce acidic, mainly sulfated, glycoconjugates with Galβ1,3GalNAc residues (Type 2) or glucosidic and/or mannosidic residues (Types 3 and 4). In sialylated chains, terminal sialic acid is bound to the penultimate GalNAc and Galβ1,3GalNAc residues. The complex composition of the mucus produced by epidermal gland cells of B. luctuosum may be correlated with its different functions. Ultrastructural studies of the epidermal gland cells showed differing morphology, and the presence in the gland cells of Types 3 and 4 of a funnel-shaped structure for the extrusion of the secretory material. 相似文献
17.
We have examined the specificity of communication between cells in culture by co-culturing cells derived from mammalian, avian, and arthropod organisms. Both mammalian and avian culture cells have similar gap junctional phenotypes, while the insect (arthropod) cell lines have a significantly different gap junctional structure. Electrophysiological and ultrastructural methods were used to examine ionic coupling and junctional interactions between homologous and heterologous cell types. In homologous cell systems, gap junctions and ionic coupling are present at a high incidence. Also, heterologous vertebrate cells in co-culture can communicate readily. By contrast, practically no coupling (0-8%) is detectable between heterologous insect cell lines (Homopteran or Lepidopteran) and vertebrate cells (mammalian myocardial or 3T3 cells). No gap junctions have been observed between arthropod and vertebrate cell types, even though the heterologous cells may be separated by less than 10 nm. In additional studies, a low incidence of coupling was found between heterologous insect cell lines derived from different arthropod orders. However, extensive coupling was detected between insect cell lines that are derived from the same order (Homoptera). These observations suggest that there is little or no apparent specificity for communication between vertebrate cells in culture that express the same gap junctional phenotype, while there is a definite communication specificity that exists between arthropod cells in culture. 相似文献
18.
Summary Five categories of granulated cells were distinguished by their ultrastructural features, and quantitative analyses were made of the pars distalis cells in normal and castrated lizards. The gonadotropin-producing cell was identified on the basis of its uniform distribution in the gland as well as from cytological changes resulting from castration. The secretory granules of the gonadotropic cell vary in size (100–500 m) and density, and lipid bodies are commonly present. Following castration, the endoplasmic reticulum proliferates, forming many small, rough-surfaced, dilated cisternae which do not coalesce greatly as in other vertebrate species. Degranulation is accompanied by hypertrophy and hyperplasia of the mitochondria and by the appearance in the cytoplasm of conspicuous clusters of microfilaments. The designated gonadotropic cell was the only class of secretory cell showing consistent changes following three weeks of castration.In addition to the uniformly distributed gonadotrope cell, two secretory cells occur mainly in the rostral half of the gland, and two in the caudal half. Tentative identification of the cell types is discussed in the light of available information on the localization of the hormones in the pars distalis of this species.Grateful recognition is given to the Electron Microscope Laboratory of the University of California, Berkeley, for use of their facilities, and to Emily Reid for her assistance with the illustrations.Member of Consejo Nacional de Investigaciones Cientificas y Técnicas. 相似文献
19.
R. Lenke 《Journal of fish biology》1991,39(3):383-392
A histological and ultrastructural examination of the opercular gland of the cleaner-wrasse Labroides dimidiutus has demonstrated that the slimy envelope which covers the animal at night is mainly produced by large mucous goblet cells in a folded epithelium; there is a high number of acidophilic secretory cells. At the ultrastructural level, both cell-types open at the surface of the epidermis. Preliminary experiments with pilocarpine, atropine and the α-blocker propranolol reveal a neural regulation of mucus discharge. Also, the rich vascularization of the gland could indicate a hormonal control e.g. by prolactin. The secretory products of both types of goblet cells are discussed in relation to their possible antibiotic properties. 相似文献
20.
W. Giard J.-M. Lebel E. Boucaud-Camou P. Favrel 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1998,168(2):81-86
In relation with the digestive cycle, the digestive gland cells of bivalve molluscs undergo a sequence of cytological changes
which is controlled by external and internal effectors such as putative gastrointestinal hormones and growth differentiation
factors. A tissue dissociation method was developed to investigate the in vitro effect of the vertebrate growth and differentiation
factors: insulin, insulin growth factor I (IGF-I), basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF)
on the digestive gland cells of the scallop Pecten maximus. All these vertebrate peptides induced a dose-dependent increased incorporation of 3H-leucine and 14C-uridine in whole digestive gland cell suspensions. However, after Percoll density gradient purification of the digestive
cells, only stem and undifferentiated enriched cell fractions were responsive to the different peptides. In addition, insulin
and IGF-I, but not EGF and bFGF, stimulated 3H-leucine incorporation in control dispersed mantle edge cells. These results suggest that insulin-related peptides could
work as general growth promoting factors in molluscs. On the other hand, EGF and bFGF, or at least their molluscan counterparts,
may be efficient growth differentiation factors in the regenerative processes occurring in the digestive gland of molluscs.
Accepted: 26 September 1997 相似文献