Measuring stoat (

Comparisons were made of density indices of free-living populations of ship rats (Rattus rattus) in mixed forest in New Zealand by using footprint tracking tunnels and two kill-trapping methods. Tracking tunnels and snap-trap removal indices of rat densities showed similar trends when run on a 9 ha trapping grid, although immigration onto the grid occurred, thus violating one of the assumptions of the analysis. Tracking rates and snaptrap capture rates were not significantly correlated when run along a trapping line for a 12 month period, although tracking rates and the total number of rats caught in a trapping session were significantly correlated. Time series analysis showed that rat density indices from tracking tunnels and Fenn traps were significantly correlated when run for 27 consecutive months in a rat population with moderate density, but were not correlated in a low density rat population. The findings highlight the importance of habitat, sample size and target species behaviour in influencing relative density indices obtained from tracking tunnels, snap-traps and Fenn traps. Given the widespread use of rodent tracking tunnels in New Zealand, we suggest that tracking tunnels should only be used to compare relative abundance within similar habitat types, and should always be complemented with a second density index. The relationship between the commonly used density indices and true rodent population density requires urgent attention.     

Effect of grazing on ship rat density in forest fragments of lowland Waikato, New?Zealand

Ship rat (Rattus rattus) density was assessed by snap-trapping during summer and autumn in eight indigenous forest fragments (mean 5 ha) in rural landscapes of Waikato, a lowland pastoral farming district of the North Island, New?Zealand. Four of the eight were fenced and four grazed. In each set of four, half were connected with hedgerows, gullies or some other vegetative corridor to nearby forest and half were completely isolated. Summer rat density based on the number trapped in the first six nights was higher in fenced (mean 6.5 rats?ha^?1) than in grazed fragments (mean 0.5 rats?ha^?1; P?=?0.02). Rats were eradicated (no rats caught and no rat footprints recorded for three consecutive nights) from all eight fragments in January?April 2008, but reinvaded within a month; time to eradication averaged 47 nights in fenced and 19 nights in grazed fragments. A second six-night trapping operation in autumn, 1?3 months after eradication, found no effect of fencing (P?=?0.73). Connectedness to an adjacent source of immigrants did not influence rat density within a fragment in either season (summer P?=?0.25, autumn P?=?0.67). An uncalibrated, rapid (one-night) index of ship rat density, using baited tracking tunnels set in a 50 ? 50 m grid, showed a promising relationship with the number of rats killed per hectare over the first six nights, up to tracking index values of c.?30% (corresponding to c.?3?5 rats ha^?1). The index will enable managers to determine if rat abundance is low enough to achieve conservation benefits. Our results confirm a dilemma for conservation in forest fragments. Fencing protects vegetation, litter and associated ecological processes, but also increases number of ship rats, which destroy seeds, invertebrates and nesting birds. Maximising the biodiversity values of forest fragments therefore requires both fencing and control of ship rats.     

Large-Scale Poisoning of Ship Rats (

This paper describes the impact of nine poison operations on ship rats in four areas (35 ha to 3200 ha) of North Island forest. Poisoning with 1080, brodifacoum, or pindone killed 87- 100% of rats, based on trapping and tracking-tunnel indices. Rat populations took 4-5 months to recover. Operations to protect nesting birds should therefore coincide with the onset of nesting and be rePeated each year, although not necessarily with the same methods. Population reduction declined each year at Mapara, King Country, during three annual 1080 operations which used the same lures and baits, but remained high at Kaharoa, Bay of Plenty, where poison toxicity was higher, non- toxic bait was pre-fed, and poisoning methods varied each year. Mouse tracking rates increased in poisoned forests 3-6 months after poisoning if the initial kill of rats exceeded 90%, Peaked 7-9 months after poisoning, then declined to pre-poison levels. Future research should focus on how prey and non-prey species within a forest community respond to a temporary reduction in rat numbers, and on methods to maintain low rat densities after initial knock-down.     

Response of mice (Mus musculus) to the removal of black rats (Rattus rattus) in arid forest on Santa Cruz Island, Galápagos

A combined rat and mouse trapping grid was established in arid coastal forest on Santa Cruz Island, Galápagos over June and July 2009. Mouse traps were opened first and did not initially catch any mice. Rat traps were opened four days later and began catching rats on the second night. Mice were trapped in increasing numbers only after the rat catch-rate had declined substantially. Interference with bait at rat traps also increased. The estimated density of rats was 4.8 rats/ha and mice 32.3/ha. The results suggest mice were present but their activity and/or numbers were being suppressed by the larger rodent. This conclusion has implications for competitive exclusion of native Galápagos rodents by both introduced species. It also suggests caution for rodent abundance estimates and planned rodent eradications when two or more species are present.     

Trappability and densities of stoats (

Stoat (Mustela erminea) density was estimated by live-trapping in a South Island Nothofagus forest, New Zealand, at 8-9 (Jan/Feb 1996) and 15-16 (Aug/Sep 1996) month intervals after significant beech seedfall in autumn 1995. Absolute densities were 4.2 stoats per km² (2.9-7.7 stoats per km², 95% confidence intervals) in Jan;Feb 1996 and 2.5 stoats per km² (2.1-3.5 stoats per km²) in Aug/Sep 1996. Trappability of stoats increased in the latter sampling period, probably because mice (Mus musculus) had become extremely scarce. accordingly, trapping rates of stoats may vary temporally and spatially with food supply rather than only with absolute abundance. Ship rats (Rattus rattus) capture rates doubled between Jan/Feb 1996 and Aug/Sep 1996, but rapidly declined shortly afterwards. Trappability of ship rats also increased in the latter sampling period. These factors must be considered when planning methods of indexing relative densities of stoats and rats.     

Responses of kukupa (

The kukupa or New Zealand pigeon (Hemiphaga novaeseelandiae) is gradually declining on the New Zealand mainland, due mostly to predation by introduced pest mammals including ship rats (Rattus rattus) and brushtail possums (Trichosurus vulpecula). We report on a co-operative project between Maori landowners, the Department of Conservation, and Manaaki Whenua–Landcare Research researchers to restore a Northland kukupa population and to examine kukupa nesting success in relation to pest abundance. Ship rats and possums were targeted by trapping and poisoning throughout Motatau Forest (350 ha) from 1997 to 1999; only possums were targeted in 2000. All 13 kukupa nests located before pest control started in late 1997 failed at the egg stage, but all seven nests located in 1998–99 successfully fledged young when trapping and tracking indices of possums and ship rats were less than 4%. After pest control, counts of kukupa and some other bird species increased at Motatau compared with counts in a nearby non-treatment block, suggesting numbers of adult kukupa can be increased in small forest areas by intensive pest control. This increase is due at least partly to increased nest success. Evidence from time-lapse video cameras, sign remaining at nests, and nest success rates under different pest control regimes suggest both ship rats and possums are important predators at kukupa nests.     

The effectiveness of poison bait stations at reducing ship rat abundance during an irruption in a Nothofagus forest

Abstract

Ship rats exhibit large increases in abundance (irruptions) following heavy beech seedfall in New Zealand's Nothofagus forests. Predation by rats at high density severely damages native fauna populations. In 2006 the Department of Conservation undertook a management experiment in the Eg‐linton Valley to see if they could protect endangered species during a rat irruption. Poison (0.15% 1080, followed by 0.0375% coumatetralyl, or Racumin®) was laid in bait stations, and the consequences for rat abundance and survival were estimated. All 10 radio‐tagged rats died, suggesting that 1080 had a high impact on the rat population. The two rats that made the smallest daily movements survived longer than the others. Live trapping documented a reduced abundance of rats within the poison area (450 ha) after 1 month of poisoning. However, after 4 months of poisoning, the abundance of rats had begun to recover. Further investigation is needed on acceptance of Racumin® to rats, optimum spacing of bait stations for rats, and bait competition between rats and mice when densities of both species are high.     

Does the house mouse self-regulate its density in maturing sorghum and wheat crops?

1. One of the central questions in population ecology and management is: what regulates population growth? House mouse Mus domesticus L. populations erupt occasionally in grain-growing regions in Australia. This study aimed to determine whether mouse populations are self-regulated in maturing sorghum and wheat crops. This was assessed by examining food supply to mice (i.e. yield) and the relationship between initial mouse density (D(I)) and density at harvest (D(H)). Eight levels of D(I) ranging from 89 to 5555 mice ha(-1) were introduced to sorghum at the hard dough stage and to wheat crops at the milky stage in mouse-proofed pens. D(H) was measured by trapping out mice 49 days after the introduction. 2. There were at least 3.11 tonnes ha(-1) of wheat and 1.85 tonnes ha(-1) of sorghum grain available for mice at harvest. The estimated relationship between D(I) and D(H) was asymptotic exponential, with D(H) initially increasing almost linearly with D(I). When D(I) was above c. 500 mice ha(-1), D(H) increased asymptotically with D(I) and then saturated at c. 3100 mice ha(-1). The asymptotic increases in and saturation of D(H) was due partly to more young mice being born and recruited in pens treated with lower levels of D(I). 3. Our findings indicated that mouse densities in maturing cereal crops were driven by a numerical response of mice to the abundant supply of grain, modified by some unknown self-regulation mechanism that reduced this numerical response of mice at higher mouse densities. The mechanism was possibly spacing behaviours. Although the nature of this self-regulation mechanism is not known our model is, nevertheless, useful for predicting increases and eruptions in mouse population density in sorghum and wheat crops. Understanding the nature of this mechanism may provide insights into population processes that can be exploited in controlling mice in cereal crops.     

Estimating density of ship rats in New Zealand forests by capture- mark-recapture trapping

We developed a capture-mark-recapture protocol for measuring the population density (D) of ship rats (Rattus rattus) in forest. Either mesh cage traps or Elliott box traps were set at each of six sites (48 traps per site for 5 nights) in the Orongorongo Valley on two occasions in autumn 2003. Cage traps only were set at three sites in autumn 2004. Rats were caught much more readily in cage traps than in Elliott traps and none were recaptured in Elliott traps. Additional food, bedding and trap covers reduced mortality and interference with traps. To estimate density we fitted a spatial detection model; this method avoids the need to estimate effective trapping area. Estimates were based on both a model assuming equal capture probability (Dˆ₀) and a model incorporating temporal and individual variation (Dˆ_th). Our target for precision was CV(Dˆ) ≤ 20%, but when data were pooled from multiple sites with cage traps, CV(Dˆ_th) was ~30%. Estimated density of rats (Dˆ_th) was 5 ha^-1in 2003 and 9 ha^-1 in 2004; these estimates did not differ significantly. The overall capture index in 2004 was 3 rats per 00 corrected trap-nights on snap-trap lines set after live trapping. House mice were caught in both types of live trap, but at rates high enough for density estimation only where Elliott traps were used. Field estimates of detection functions for rats captured with cage traps allowed us to simulate the performance of alternative trapping systems. We predict that a 64-trap layout at three sites with five trapping occasions would yield acceptable precision of Dˆ_th (20–23%) at the observed rat densities. Our use of Dˆ_th was conservative; slightly higher precision may be achieved by assuming constant trappability ( Dˆ₀), and future work may justify this assumption.     

Preference and performance of a gall-inducing sawfly: plant vigor, sex, gall traits and phenology

Four hypotheses regarding the role of predation in the population dynamics of eruptive small mammal communities were tested using the small mammal assemblage found in mixed forests in New Zealand. Large-scale (750 ha) predator removal was conducted, targeting stoats ( Mustela erminea ). House mouse ( Mus musculus ) and ship rat ( Rattus rattus ) population dynamics during an eruption were compared in areas with and without predator reduction. The success of predator reduction was measured by comparing live-capture rates of predators on treatment and non-treatment areas, and by recruitment rates of the threatened northern brown kiwi ( Apteryx australis mantelli ). Overall, predator reduction was successful, although there was a continual low rate of reinvasion. The predictions and results were that 1) Predators can slow but not prevent a population eruption. Supported: Populations of mice and rats erupted to high densities in areas with and without predator reduction, following synchronous southern beech ( Nothofagus spp.) seeding. 2) Predators cannot truncate peak prey population size. Supported: Peak densities of mice and rats were not significantly different between treatment and non-treatment areas. 3) Predators can hasten the rate of decline in prey populations during the crash phase. Not supported: There was evidence of populations of mice and rats declining slower in areas with predators removed, but none of the trends were significant. 4) Predators can limit low-phase prey populations. Equivocal: Populations of rats in beech forest, and population of mice and rats in coastline habitats were significantly higher in areas with predators removed, but were not significantly different in tawa-podocarp forest. Therefore, the role of food in driving the early stages of the mouse and rat eruption was demonstrated, but the role of predation in the decline and low phases is unclear.     

Home ranges of ship rats in a small New Zealand forest as revealed by trapping and tracking

Abstract

The home ranges of 5 ship rats (Rattus r. rattus L.) in a small forest area near Palmerston North were determined for 7 months by concurrent cage-trapping and smoked paper tracking. Baited tracking platforms were over 20 times as effective as cage-trapping in obtaining location data, and home ranges revealed by tracking were on average 5 times the area of trap-revealed home ranges. All the rats were to some extent cage-trap shy. However, although cage-traps could not supply useful information on range boundaries or swift acknowledgment of boundary changes, centres of activity calculated from trap data were comparable with those from tracking data. Tracking rates of individuals were variable; it would be risky to assume that they accurately reflected intensity of use of any area over short periods or when rats may have been competing for baits. The rats had stable home ranges; 3 females had ranges predominantly exclusive to each other. The progressive removal of individuals from the study area effected the prompt expansion of adjacent ranges to include vacated areas; that is, range size was inversely related to rat density.     

Large-scale rodent control reduces pre- and post-dispersal seed predation of the endangered Hawaiian lobeliad, Cyanea superba subsp. superba (Campanulaceae)

Large-scale rodent control can help to manage endangered species that are vulnerable to invasive rodent consumption. A 26?ha rodent snap-trap grid was installed in montane forest on Oahu Island, Hawaii, in order to protect endangered snails and plants. To assess the effectiveness of this trapping operation in reducing fruit consumption and seed predation of the endangered Hawaiian lobeliad, Cyanea superba subsp. superba, pre- and post-dispersal C. superba fruit consumption were monitored for 36 plants at the site with rodent control (Kahanahaiki) and 42 plants at an adjacent site without rodent control (Pahole). Over 47?% of all monitored fruit were eaten on the plants at Pahole compared to 4?% at Kahanahaiki. Images captured using motion-sensing cameras suggest that black rats (Rattus rattus) were the only pre-dispersal fruit consumers. To quantify post-dispersal fruit consumption, and to identify the culprit frugivore(s), mature fruit were placed in tracking tunnels positioned on the forest floor and checked daily. At Pahole, all of the fruit were consumed by rats compared to 29?% at Kahanahaiki. Lastly, to determine if rodents from the sites were predators or dispersers of C. superba seed, fruit were fed to captive black rats and house mice (Mus musculus). Black rats consumed entire fruit, killing all the seed, while mice did little damage to the fruit and seed. Therefore, large-scale rat trapping can directly benefit the reproduction of C. superba subsp. superba. Controlling black rats at restoration sites appears integral to the successful restoration of this endangered plant species.     

Hoarding behavior by ship rats (Rattus rattus) in captivity and its relevance to the effectiveness of pest control operations

Hoarding of food items is well known among muroid rodents, but evidence for hoarding behavior among ship rats (Rattus rattus) is scant. Here, we characterize hoarding behavior in ship rats maintained in captivity after capture from the wild. After acclimatization to captivity, 40 ship rats (21 females, 19 males) were presented with baits in experiments designed to emulate a typical poison control operation for vertebrate pests in New Zealand: this involved first offering rats nontoxic cereal baits (of 2- or 6-g size) as a prefeed for three nights consecutively, followed by 6- or 12-g cereal baits laden with 0.15% 1080 on the fourth night. Seventy-eight percent of rats (31/40) hoarded food in distinct cache sites when presented with nontoxic baits although there was no significant effect of bait size or type on hoarding behavior and nor did hoarding behavior vary according to rat gender. When rats were presented with 1080-laden baits, the incidence of hoarding was reduced to 40%, due to the onset of toxicosis. This study indicates that R. rattus will show hoarding behavior analogous to other rat species when presented with an excess of cereal-based baits, at least under conditions of captivity and free from competition. This finding may have practical relevance: since 1080 is the principal toxin used against the major vertebrate pest species in New Zealand (the brushtail possum, Trichosurus vulpecula), ship rats have the potential to deplete supplies of prefeed and/or toxic baits intended for possum control. However, based on typical rat densities recorded in New Zealand native forest (c. 5 rats/ha), the degree of removal and manipulation of toxic baits observed by ship rats here is unlikely to impact adversely on the efficacy of possum control operations.     

Toxicity of cholecalciferol to rats in a multi-species bait

The effectiveness of Feracol?, a possum control paste bait containing 0.8% cholecalciferol, as a rodenticide has been assessed in cage and field trials. Caged rats were provided with toxic bait in choice and no-choice tests. Feracol? was readily eaten when presented as the sole food source or with other food, and was effective at killing rats in both situations. Wild-caught and laboratory rats (n =?35), comprising both ship (Rattus rattus) and Norway rats (R.?norvegicus), were presented with 30 g of Feracol? alone or with an equivalent toxic bait over 48 h. Thirty-four rats died in an average of 4.0 days. Having established that the paste, originally designed for possum control, is also an effective rodenticide for rat control, field trials were initiated with the paste delivered in the field in Philproof? and Striker? bait stations. Monitoring of rat numbers before and after application of toxic bait was undertaken at three trial sites, Lions Hut, Mangaone and Pakoakoa, in Te Urewera National Park in the North Island of New?Zealand. Rat population density was assessed using tracking tunnels. Philproof? bait stations containing 200 g of Feracol? were placed 50 m apart on grids at Lions Hut and monitoring was undertaken at one location per hectare using tracking tunnels. At Mangaone and Pakoakoa, two Striker? bait stations containing 18 g of Feracol? were sited at 25-m intervals on lines 150 m apart, and monitoring was undertaken with five lines of 10 tunnels at 50-m intervals. At Lions Hut, rat tracking decreased from 78% to 3% of tunnels tracked; at Mangaone the reduction was 51% to 0%; and at Pakoakoa from 36% to 0%. These trials demonstrate that Feracol? is effective at reducing both moderate and high concentrations of ship rats in the Philproof? and Striker? bait station delivery systems.     

Who’s in the hood? Assessing a novel rodent deterrent at pest fencing in New Zealand

ABSTRACT

Fenced ecosanctuaries may reduce predator presence at reserves by incorporating deterrents into pest management programmes. We quantified rodent visits to the steel hood of ecosanctuary fencing and illuminated our experimental sites to assess whether light could deter ship rats (Rattus rattus) and house mice (Mus musculus). Additionally, we assessed how mice used fencing in the presence and absence of ship rats by utilising two types of fence sections: one accessible to mice alone (along fencing within an ecosanctuary) and one accessible to mice and rats (at perimeter fence sites opposite pasture or forest habitat). We monitored sites using cameras and tracking cards within the fence hood and at tunnels at the fence base. Along the internal fence, mice were never observed within the hood but marked 40% of tunnels at the fence base. Along the perimeter fence, mice made four visits to the hood and marked 28% of tunnels at the fence base. Rats travelled exclusively within the fence hood (n?=?42). Light did not reduce rat sightings but adjacent habitat affected their presence (forest > pasture; p?≤?0.01). Positioning future ecosanctuary fencing alongside pasture and maintaining open corridors opposite fences at current ecosanctuaries may reduce rat presence.     

Quantification of the hepatic contribution to the catabolism of high density lipoproteins in rats.

Isolated rat livers were perfused for four hours in a recirculating system containing washed rat erythrocytes. Biologically screened radioiodinated rat high density lipoproteins (1.090 < d < 1.21 g/ml) were added to the perfusate with different amounts of whole serum to supply unlabeled rat high density lipoproteins. The protein moiety of the lipoprotein contained more than 95% of the radioiodine. The fraction of apolipoprotein mass degraded during the perfusion was quantified by the linear increment of non-protein-bound radioiodine in the perfusate, corrected for the increment observed during recirculation of the perfusate in the absence of a liver. The small amount of (131)I secreted into bile was added to calculate the fractional catabolic rate. The fractional catabolic rate ranged from 0.22 to 0.63% per hour in 12 experiments and was inversely related to the size of the perfusate pool of high density apolipoprotein. The absolute catabolic rate of high density apolipoprotein (fractional catabolic rate x pool size) in three livers in which the concentration of rat HDL in the perfusate approximated that in intact rats was 69.5 +/- 10.4 micro g hr(-1) (mean +/- SD). The rate of disappearance of cholesteryl esters of rat high density lipoproteins (labeled biologically by injecting donor rats with [5-(3)H]mevalonic acid) from the liver perfusate did not exceed that of the apoprotein component. These rates were compared with catabolic rates for rat high density lipoproteins in intact rats. Fractional catabolic rate in vivo, obtained by multicompartmental analysis of the disappearance curve of (131)I-high density apolipoprotein from blood plasma, was 11.9 +/- 1.3% hr(-1) (mean +/- SD). Total catabolic rate in vivo (fractional catabolic rate x intravascular pool of high density apolipoprotein) was 986 +/- 145 micro g hr(-1) (mean +/- SD). The results suggest that only a small fraction of high density lipoproteins in blood plasma of rats is degraded directly by the liver.-Sigurdsson, G., S-P. Noel, and R. J. Havel. Quantification of the hepatic contribution to the catabolism of high density lipoproteins in rats.     

Discriminating the Drivers of Edge Effects on Nest Predation: Forest Edges Reduce Capture Rates of Ship Rats (Rattus rattus), a Globally Invasive Nest Predator,by Altering Vegetation Structure

Forest edges can strongly affect avian nest success by altering nest predation rates, but this relationship is inconsistent and context dependent. There is a need for researchers to improve the predictability of edge effects on nest predation rates by examining the mechanisms driving their occurrence and variability. In this study, we examined how the capture rates of ship rats, an invasive nest predator responsible for avian declines globally, varied with distance from the forest edge within forest fragments in a pastoral landscape in New Zealand. We hypothesised that forest edges would affect capture rates by altering vegetation structure within fragments, and that the strength of edge effects would depend on whether fragments were grazed by livestock. We measured vegetation structure and rat capture rates at 488 locations ranging from 0–212 m from the forest edge in 15 forest fragments, seven of which were grazed. Contrary to the vast majority of previous studies of edge effects on nest predation, ship rat capture rates increased with increasing distance from the forest edge. For grazed fragments, capture rates were estimated to be 78% lower at the forest edge than 118 m into the forest interior (the farthest distance for grazed fragments). This relationship was similar for ungrazed fragments, with capture rates estimated to be 51% lower at the forest edge than 118 m into the forest interior. A subsequent path analysis suggested that these ‘reverse’ edge effects were largely or entirely mediated by changes in vegetation structure, implying that edge effects on ship rats can be predicted from the response of vegetation structure to forest edges. We suggest the occurrence, strength, and direction of edge effects on nest predation rates may depend on edge-driven changes in local habitat when the dominant predator is primarily restricted to forest patches.     

Pest fencing or pest trapping: A bio‐economic analysis of cost‐effectiveness

Scofield et al. discredited the utility of pest‐exclusion fences for restoring biodiversity partly on the grounds of unquantified costs and benefits. We estimated the discounted costs of mammal exclusion fences, semi‐permeable (‘leaky’) fences and trapping, over 50 years and adjusted costs by their observed effectiveness at reducing mammalian predator abundance. We modelled data from two large predator management programmes operated by the New Zealand Department of Conservation. Using typical baseline costs and predator control efficacies (scale 0 to 1), the model predicted that an exclusion fence (efficacy 1.0) is the cheapest and most cost‐effective option for areas below about 1 ha, a leaky fence (efficacy 0.9) is most cost‐effective for 1–219 ha, and trapping (efficacy 0.6, based on 0.2 traps per hectare and a 1500‐m buffer to reduce predator reinvasion) for areas above 219 ha. This ranking was insensitive to adjustments in efficacy, but reducing efficacy of leaky fences to 0.8 or increasing trapping efficacy to 0.7 reduced the cost‐effective range of leaky fences by about 90 ha. Reducing trap maintenance costs from $300 to $100 per trap per year (e.g. using long‐life lures), or reducing trap buffer widths to 500 m, significantly elevated trapping as the most cost‐effective method for areas greater than 11–15 ha. These results were largely consistent with an ecological measure of effectiveness based on observed rates of recovery of two indigenous skink species inside exclusion fences or with trapping. The results support criticisms that exclusion fences are generally not cost‐effective, but highlight the value of considering cheaper leaky designs for small‐ to medium‐sized areas. Because this study is based largely on reductions in predator abundance, it has general application to broader biodiversity protection interests, but not to indigenous species that are highly sensitive to predation and only ever adequately protected on the mainland by exclusion fences.     

Mass trapping of Prays nephelomima (Lepidoptera: Yponomeutidae) in citrus orchards: optimizing trap design and density

The moth Prays nephelomima (Meirick) (Lepidoptera: Yponomeutidae) is a significant pest of citrus (Citrus spp.), and the recent identification of the female sex pheromone has enabled new direct control tactics to be considered. Six trap designs were compared for suitability in mass trapping, and Pherocon III delta traps were chosen to further evaluate mass trapping. A mass trapping field trial was carried out at five lemon, Citrus limon L., orchards to determine the effect of trap density on catch and rind spot damage on fruit. One plot (0.33-1.0 ha) of each of the five trap density treatments (3, 10, 30, 100, and 300 traps/ha) were operated at each orchard over 12 wk. Catch per trap was reduced as trap density increased and a mean of 12,000 and 16,000 males per ha were killed at the trap densities of 100 and 300 traps per ha, respectively. Increased trap density reduced the percentage of flowers infested with P. nephelomima larvae and reduced the number of moths emerging from flowers. The incidence of rindspot damage on fruit decreased from 45 to 16% as the density of traps increased from 3 to 100 traps per ha. Incidence (percentage of fruit with rindspot) and severity (number of rindspots per fruit) was similar at 100 and 300 traps per ha, indicating that the optimal trap density for reducing rindspot damage is likely to be between 30 and 100 traps per ha. Prospects for converting mass trapping to a lure and kill system are discussed.     

Catabolism of the apoprotein of low density lipoproteins by the isolated perfused rat liver.

Isolated rat livers were perfused for 4 hours in a recirculating system containing washed rat erythrocytes. Biologically screened, radioiodinated low density lipoproteins (1.030 < d < 1.055 g/ml) were added to the perfusate with different amounts of whole serum to supply unlabeled rat low density lipoproteins. Apolipoprotein B contained 90% of the bound (131)I, other apolipoproteins contained 4%, and lipids contained the remainder. The fraction of apolipoprotein mass degraded during the perfusion was quantified by the linear increment of non-protein-bound radioiodine in the perfusate, corrected for the increment observed during recirculation of the perfusate in the absence of a liver. The fractional catabolic rate ranged from 0.3 to 1.7%/hr in seven experiments and was inversely related to the size of perfusate pool of low density apolipoprotein. The catabolic rate of low density apolipoprotein (fractional catabolic rate x pool size) in four livers, in which the concentration of rat low density lipoproteins was 50-100% of that present in intact rats, was 5.3 +/- 2.7 micro g hr(-1) (mean +/- SD). Similar results were obtained with human low density lipoproteins. These rates were compared with catabolic rates for the apoprotein of rat low density lipoproteins in intact animals. Fractional catabolic rate in vivo, obtained by multi-compartmental analysis of the disappearance curve of (131)I-labeled low density apolipoprotein from blood plasma, was 15.2 +/- 3.1% hr(-1) (mean +/- SD). Total catabolic rate in vivo (fractional catabolic rate x intravascular pool of low density apolipoprotein) was 76 +/- 14 micro g hr(-1) (mean +/- SD). The results suggest that only a small fraction of low density apolipoprotein mass in rats is degraded by the liver.