共查询到20条相似文献,搜索用时 15 毫秒
1.
2.
3.
4.
5.
Anti‐Inflammatory Effect of Ascochlorin in LPS‐Stimulated RAW 264.7 Macrophage Cells Is Accompanied With the Down‐Regulation of iNOS,COX‐2 and Proinflammatory Cytokines Through NF‐κB,ERK1/2, and p38 Signaling Pathway 下载免费PDF全文
Sook‐Hyun Lee Choong‐Hwan Kwak Sung‐Kyun Lee Sun‐Hyung Ha Junyoung Park Tae‐Wook Chung Ki‐Tae Ha Suk‐Jong Suh Young‐Chae Chang Hyeun Wook Chang Young‐Choon Lee Bong‐Seok Kang Junji Magae Cheorl‐Ho Kim 《Journal of cellular biochemistry》2016,117(4):978-987
6.
7.
8.
Rosa M. Guzzo PhD Jason Gibson Ren‐He Xu Francis Y. Lee Hicham Drissi PhD 《Journal of cellular biochemistry》2013,114(2):480-490
Induced pluripotent stem cells (iPSC) hold tremendous potential for personalized cell‐based repair strategies to treat musculoskeletal disorders. To establish human iPSCs as a potential source of viable chondroprogenitors for articular cartilage repair, we assessed the in vitro chondrogenic potential of the pluripotent population versus an iPSC‐derived mesenchymal‐like progenitor population. We found the direct plating of undifferentiated iPSCs into high‐density micromass cultures in the presence of BMP‐2 promoted chondrogenic differentiation, however these conditions resulted in a mixed population of cells resembling the phenotype of articular cartilage, transient cartilage, and fibrocartilage. The progenitor cells derived from human iPSCs exhibited immunophenotypic features of mesenchymal stem cells (MSCs) and developed along multiple mesenchymal lineages, including osteoblasts, adipocytes, and chondrocytes in vitro. The data indicate the derivation of a mesenchymal stem cell population from human iPSCs is necessary to limit culture heterogeneity as well as chondrocyte maturation in the differentiated progeny. Moreover, as compared to pellet culture differentiation, BMP‐2 treatment of iPSC‐derived MSC‐like (iPSC–MSC) micromass cultures resulted in a phenotype more typical of articular chondrocytes, characterized by the enrichment of cartilage‐specific type II collagen (Col2a1), decreased expression of type I collagen (Col1a1) as well as lack of chondrocyte hypertrophy. These studies represent a first step toward identifying the most suitable iPSC progeny for developing cell‐based approaches to repair joint cartilage damage. J. Cell. Biochem. 114: 480–490, 2013. © 2012 Wiley Periodicals, Inc. 相似文献
9.
Diversity of Hepatocellular Carcinoma Clones Bearing Hematopoietic Malignancies‐Related Chromosomal Translocation 下载免费PDF全文
Romain Parent Marie‐Laure Plissonnier Brigitte Bancel Wan‐Li Liao Sylvie Rumin Remal Asaad Marianne Till Damien Sanlaville Fabien Zoulim Christian Trépo Marie‐Jeanne Marion 《Journal of cellular biochemistry》2014,115(4):666-677
10.
11.
Kristen P. McKenzie Dana C. Mayer Jane E. Aubin 《Journal of cellular biochemistry》2013,114(5):1066-1073
The identification and purification of murine multipotent mesenchymal stem cells (MSCs) have been difficult due to their low frequency, the presence of contaminating cell types and lack of unambiguous markers. Using a magnetic micro‐beads negative selection technique to remove hematopoietic cells from mouse bone marrow stromal cells (BMSCs), our lab recently isolated a highly purified osteoprogenitor (HipOP) population that was also enriched for other mesenchymal precursors, including MSCs [Itoh and Aubin, 2009 ]. We now report that HipOPs are also highly enriched in vascular endothelial cells (VECs), which we hypothesized were an accessory cell type regulating osteogenesis. However, when VECs were immunodepleted from HipOPs with anti‐CD31 antibodies, the resulting CD31(?) HipOP population had equal osteogenic capacity to the HipOPs in vitro and in vivo. Analysis of gene expression of Ncad, Pth1r, Ang1, Cxcl12, Jag1, Pdgfr‐β, α‐sma, Desmin, and Ng2 suggested that both HipOPs and CD31(?) HipOPs are hemopoietic stem cell (HSC) niche populations. However, the data support the view that osteoblast differentiation and depletion of VECs modulate the HSC niche. J. Cell. Biochem. 114: 1066–1073, 2013. © 2012 Wiley Periodicals, Inc. 相似文献
12.
13.
14.
15.
16.
17.
CD49f‐Positive Testicular Cells in Saanen Dairy Goat Were Identified as Spermatogonia‐Like Cells by miRNA Profiling Analysis 下载免费PDF全文
Jiang Wu Mingzhi Liao Haijing Zhu Kai Kang Hailong Mu Wencong Song Zhiwei Niu Xin He Chunling Bai Guangpeng Li Xinping Li Jinlian Hua 《Journal of cellular biochemistry》2014,115(10):1712-1723
18.
Artificial Matrices With High‐Sulfated Glycosaminoglycans and Collagen Are Anti‐Inflammatory and Pro‐Osteogenic for Human Mesenchymal Stromal Cells 下载免费PDF全文
Ute Hempel Claudia Matthäus Carolin Preissler Stephanie Möller Vera Hintze Peter Dieter 《Journal of cellular biochemistry》2014,115(9):1561-1571
19.