共查询到20条相似文献,搜索用时 15 毫秒
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Osteosarcoma Cells Enhance Angiogenesis Visualized by Color‐Coded Imaging in the In Vivo Gelfoam® Assay 下载免费PDF全文
Fuminari Uehara Yasunori Tome Shinji Miwa Yukihiko Hiroshima Shuya Yano Mako Yamamoto Sumiyuki Mii Hiroki Maehara Michael Bouvet Fuminori Kanaya Robert M. Hoffman 《Journal of cellular biochemistry》2014,115(9):1490-1494
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《Redox report : communications in free radical research》2013,18(4):161-171
AbstractYeast flavohemoglobin (Yhb1p) plays a pivotal role in NO? detoxification and has also been implicated in oxidative/reductive stress responses. In this study, we have used a YHB1-GFP reporter expressing a full-length chromosome-tagged Yhb1-GFP fusion protein to monitor changes in flavohemoglobin levels after cell treatment with oxidants, antioxidants and nitric oxide donors. Only nitric oxide donors were found to induce a dose-dependent increase in Yhb1-GFP expression while hydrogen peroxide, menadione and diamide caused a moderate diminution of YHB1-GFP fluorescence. Additionally, the levels of endogenous and hydroperoxide-induced ROS production in the Δyhb1 mutant were comparable to those in the isogenic wild-type strain. Although peroxides increased NO? generation while nitrite and nitric oxide donors augmented ROS levels in yeast cells, their effects were generally not more pronounced in Δyhb1 than in wild-type cells. Taken together, these data suggest that yeast flavohemoglobin does not contribute to cross-protection against oxidative and nitrosative stress. 相似文献
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3‐Dimensional Tissue Is Formed From Cancer Cells In Vitro on Gelfoam®, But Not on MatrigelTM 下载免费PDF全文
Yasunori Tome Fuminari Uehara Sumiyuki Mii Shuya Yano Lei Zhang Naotoshi Sugimoto Hiroki Maehara Michael Bouvet Hiroyuki Tsuchiya Fuminori Kanaya Robert M. Hoffman 《Journal of cellular biochemistry》2014,115(8):1362-1367
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Enhanced Metastatic Recurrence Via Lymphatic Trafficking of a High‐Metastatic Variant of Human Triple‐Negative Breast Cancer After Surgical Resection in Orthotopic Nude Mouse Models 下载免费PDF全文
Shuya Yano Kiyoto Takehara Hiroshi Tazawa Hiroyuki Kishimoto Shunsuke Kagawa Michael Bouvet Toshiyoshi Fujiwara Robert M. Hoffman 《Journal of cellular biochemistry》2017,118(3):559-569
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Analysis of Stroma Labeling During Multiple Passage of a Sarcoma Imageable Patient‐Derived Orthotopic Xenograft (iPDOX) in Red Fluorescent Protein Transgenic Nude Mice 下载免费PDF全文
Tasuku Kiyuna Takashi Murakami Yasunori Tome Kei Kawaguchi Kentaro Igarashi Kentaro Miyake Fuminori Kanaya Arun Singh Fritz C. Eilber Robert M. Hoffman 《Journal of cellular biochemistry》2017,118(10):3367-3371
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Marta Czernik Antonella Fidanza Martina Sardi Cesare Galli Dario Brunetti Daniela Malatesta Leonardo Della Salda Kazutsugu Matsukawa Grazyna E. Ptak Pasqualino Loi 《Journal of cellular biochemistry》2013,114(1):134-143
Mesenchymal stem cells (MSCs) are an important cell population in the bone marrow microenvironment. MSCs have the capacity to differentiate in vitro into several mesenchymal tissues including bone, cartilage, fat, tendon, muscle, and marrow stroma. This study was designed to isolate, expand, and characterize the differentiation ability of sheep bone marrow‐derived MSCs and to demonstrate the possibility to permanently express a reporter gene. Bone marrow was collected from the iliac crest and mononuclear cells were separated by density gradient centrifugation. Sheep MSCs cell lines were stable characterized as CD44+ and CD34? and then transfected with a green fluorescent protein (GFP) reporter gene. The GFP expression was maintained in about half (46.6%) of cloned blastocysts produced by nuclear transfer of GFP+ sheep MSCs, suggesting the possibility to establish multipotent embryonic cells' lines carrying the fluorescent tag for comparative studies on the differentiation capacity of adult stem cells (MSCs) versus embryonic stem cells. We found that sheep MSCs under appropriate culture conditions could be induced to differentiate into adipocytes, chondrocytes, and osteoblast lineages. Our results confirm the plasticity of sheep MSCs and establish the foundation for the development of a pre‐clinical sheep model to test the efficiency and safety of cell replacement therapy. J. Cell. Biochem. 114: 134–143, 2012. © 2012 Wiley Periodicals, Inc. 相似文献
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Transcription Factors and Medium Suitable for Initiating the Differentiation of Human‐Induced Pluripotent Stem Cells to the Hepatocyte Lineage 下载免费PDF全文
Minoru Tomizawa Fuminobu Shinozaki Yasufumi Motoyoshi Takao Sugiyama Shigenori Yamamoto Naoki Ishige 《Journal of cellular biochemistry》2016,117(9):2001-2009
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Bombesin Receptor‐Activated Protein (BRAP) Modulates NF‐κB Activation in Bronchial Epithelial Cells by Enhancing HDAC Activity 下载免费PDF全文
Ying Liu Xiao‐Qun Qin Horst Christian Weber Yang Xiang Chi Liu Hui‐Jun Liu Huan Yang Jianxin Jiang Xiangping Qu 《Journal of cellular biochemistry》2016,117(5):1069-1077