The composition of air in sleeping bags

The effects of sleeping with one's head inside a conventional arctic sleeping bag at –20°C on body temperature and on the composition of air breathed were investigated. There was little effect on body temperature and concentrations of O₂ and CO₂ levelled off at approximately 16% and 4%, respectively. Changes in the composition of the air breathed by persons using a vapour-barrier sleeping bag and an experimental water-vapour-impermeable liner were also monitored. Concentrations of O₂ and CO₂ of approximately 11% and 6%, respectively, were observed. The possible effects of breathing such air on sleep and thermoregulation are discussed.     

Leaching of plasticizers from and surface characterization of PVC blood platelet bags.

The leaching of phthalate plasticizers from four types of blood platelet bags was investigated. The anticoagulant solutions used in the blood collection bags had pH values of 5.64 +/- .04 and contained no detectable amounts of phthalates. Platelet bag materials from each bag were soaked in normal salines for up to 5 days. The salines were tested for the leached phthalates from the bags but none could be found. However, di-(2-ethylhexyl) phthalate (DEHP) leached out of the PL-146 and Terumo bags into bovine calf serum used for soaking the bag materials. There was an increase in the amount of DEHP leached from about 1.1 mg at the end of one day to about 3.3 mg per gm of bag material at the end of a five day extraction with the serum. In PL-732 sets, a platelet bag made of a specialty polyolefin, the amount of DEHP leached out was less than 0.02 mg per g of bag material. CLX bags, which contained tri-(2-ethylhexyl) trimelliate (TETM) as a plasticizer, showed a negligible amount of it leaching into the calf serum. Infra-red spectra showed that PL-146 bags had been coated with a layer of a fatty acid amide while the Terumo bags contained a layer of a silicone fluid on their inner surfaces. CLX bags showed a coating of stearates, which were probably soaps of calcium or zinc. Scanning electron microscopy (SEM) showed that the inner surfaces of each brand of the bag were distinctly different morphologically. The two PVC bags were very similar whereas the surfaces morphology of PL-732 was rougher. Terumo bag had a different surface morphology than those of the other bags whereas the CLX bags had a very regular surface pattern. The exact significance of the surface morphology is not certain but excessively rough surfaces may not be desirable for the bags.     

Association of driver air bags with driver fatality: a matched cohort study

ObjectiveTo estimate the association of driver air bag presence with driver fatality in road traffic crashes.DesignMatched pair cohort study.SettingAll passenger vehicle crashes in the United States during 1990-2000 inclusive.Subjects51 031 driver-passenger pairs in the same vehicle.ResultsDrivers with an air bag were less likely to die than drivers without an air bag (adjusted relative risk 0.92 (95% confidence interval 0.88 to 0.96)). This estimate was nearly the same whether drivers wore a seat belt (adjusted relative risk 0.93) or not (0.91). Air bags were associated with more protection for women (0.88 (0.82 to 0.93)), than for men (0.94 (0.90 to 0.99)). Drivers wearing a seat belt were less likely to die than unbelted drivers (0.35 (0.33 to 0.36)). Belted drivers with an air bag were less likely to die than unbelted drivers without an air bag (0.32 (0.30 to 0.34)).ConclusionsIf the associations are causal the average risk of driver death was reduced 8% (95% confidence interval 4% to 12%) by an air bag. Benefit was similar for belted and unbelted drivers and was slightly greater for women. However, seat belts offered much more protection than air bags.

What is already known on this topic

Studies have estimated that driver air bags reduce the risk of death in a road vehicle crash by 10-14%These studies disagree as to whether benefit is greater for drivers wearing a seat belt or for unbelted drivers

What this study adds

Having an air bag was associated with an 8% reduction in the risk of death, whether the driver was belted or notThe reduction in risk was greater for women (12%) than for men (6%)Seat belts provided much greater protection, with seat belt use reducing the risk of death by 65% (or by 68% in combination with an air bag)     

Strategy for selecting disposable bags for cell culture media applications based on a root‐cause investigation

The use of disposable bags for cell culture media storage has grown significantly in the past decade. Some of the key advantages of using disposable bags relative to non‐disposable containers include increased product throughput, decreased cleaning validation costs, reduced risk of cross contamination and lower facility costs. As the scope of use of disposable bags for cell culture applications increases, problematic bags and scenarios should be identified and addressed to continue improving disposables technologies and meet the biotech industry's needs. In this article, we examine a cell culture application wherein media stored in disposable bags is warmed at 37°C before use for cell culture operations. A problematic bag film was identified through a prospective and retrospective cell culture investigation. The investigation provided information on the scope and variation of the issue with respect to different Chinese hamster ovary (CHO) cell lines, cell culture media, and application‐specific parameters. It also led to the development of application‐specific test methods and enabled a strategy for disposable bag film testing. The strategy was implemented for qualifying an alternative bag film for use in our processes. In this test strategy, multiple lots of 13 bag film types, encompassing eight vendors were evaluated using a three round, cell culture‐based test strategy. The test strategy resulted in the determination of four viable bag film options based on the technical data. The results of this evaluation were used to conclude that a volatile or air‐quenched compound, likely generated by gamma irradiation of the problematic bag film, negatively impacted cell culture performance. © 2013 American Institute of Chemical Engineers Biotechnol. Prog., 29:1535–1549, 2013     

DIALYSIS WITH STIRRING

Substances to be purified by dialysis are placed in collodion bags together with a toy "marble" or a bubble of air. The bags are stoppered and placed in glass tubes of a rocking machine. Distilled water of the desired temperature is circulated through the tubes (around the bags) at a rate of about 8 cc. per minute per bag while the machine is in motion. The rolling of the marbles or bubbles causes stirring which makes it possible to remove the salts from a protein solution in 24 to 48 hours.     

松萝挂袋法监测氟化盐厂大气氟污染

以长松萝为材料,制成挂袋,与石灰滤纸法（ＬＴＰ）同步对白银市某氟人盐厂内外大气氟进行监测的结果表明：两种方法在监测效果上显著相关。由松萝挂袋法监测结果确定的污染等级范围与实际情况符合,从而证明此法监测大气氟污染的可行性与可靠性。与天然地衣调查法和种子植物污染成分分析法相比,松萝挂袋法具有不受时间、地点限制,可定量反映植物聚焦污染物的数量等优点,对环境质量评价有直接参考价值。     

Aerobic and Anaerobic Storage of Single-pass,Chopped Corn Stover

Corn stover has great potential as a biomass feedstock due its widespread availability. However, storage characteristics of moist corn stover harvested from single-pass harvesters have not been well quantified. In 2007, whole-plant corn stover at 19.1–40.3% (w.b.) moisture content was stored for 237 days in aerobic piles, one covered and one uncovered, as well an anaerobic silo bag. In 2008, two stover materials—whole plant and cob/husk from 31.7% to 58.1% (w.b.) moisture—were stored for 183 or 204 days in covered and uncovered anaerobic piles, ventilated bags, or anaerobic silo bags. Stover stored in uncovered piles was rehydrated by precipitation, which increased biological activity resulting in dry matter (DM) losses from 8.2% to 39.1% with an average of 21.5%. Stover in covered piles was successfully conserved when the average moisture was less than 25% (w.b.) with DM losses of 3.3%. Stover above 36% (w.b.) moisture and piled under a plastic cover had DM losses from 6.4% to 20.2% with an average of 11.9%. Localized heating occurred in the aerobic piles when moisture was above 45% (w.b.) which lead to temperatures where spontaneous combustion might be a concern (i.e., >70°C). Ambient air blown through a center tube in the ventilated storage bag dried stover near the tube to an average of 24.2% (w.b.), but the remainder of the bag averaged 46.8% (w.b.) at removal. Loss of DM ranged from 7.4% to 22.0% with an average of 11.8% with this storage method. Stover was most successfully conserved in the bags where anaerobic conditions were maintained. Under anaerobic conditions, DM losses ranged from 0.2% to 0.9%. When anaerobic conditions were not maintained in the silo bag, DM losses averaged 6.1% of DM. Anaerobic storage is the best solution for conserving the value of moist corn stover.     

The development of an intraruminal nylon bag technique using non-fistulated animals to assess the rumen degradability of dietary plant materials

Although the conventional in situ ruminal degradability method is a relevant tool to describe the nutritional value of ruminant feeds, its need for rumen-fistulated animals may impose a restriction on its use when considering animal welfare issues and cost. The aim of the present work was to develop a ruminal degradability technique which avoids using surgically prepared animals. The concept was to orally dose a series of porous bags containing the test feeds at different times before slaughter, when the bags would be removed from the rumen for degradation measurement. Bags, smaller than those used in the conventional nylon bag technique, were made from woven nylon fabric, following two shape designs (rectangular flat shape, tetrahedral shape) and were fitted with one of three types of device for preventing their regurgitation. These bags were used in two experiments with individually housed non-pregnant, non-lactating sheep, as host animals for the in situ ruminal incubation of forage substrates. The bags were closed at the top edge by machine stitching and wrapped in tissue paper before oral dosing. Standard times for ruminal incubation of substrates in all of the tests were 4, 8, 16, 24, 48, 72 and 96 h before slaughter. The purpose of the first experiment was to compare the effectiveness of the three anti-regurgitation device designs, constructed from nylon cable ties (‘Z-shaped’, ARD1; ‘double Z-shaped’, ARD2; ‘umbrella-shaped’, ARD3), and to observe whether viable degradation curves could be generated using grass hay as the substrate. In the second experiment, three other substrates (perennial ryegrass, red clover and barley straw) were compared using flat and tetrahedral bags fitted with type ARD1 anti-regurgitation devices. Non-linear mixed-effect regression models were used to fit asymptotic exponential curves of the percentage dry matter loss of the four substrates against time of incubation in the reticulorumen, and the effect of type of anti-regurgitation device and the shape of nylon bag. All three devices were highly successful at preventing regurgitation with 93% to 100% of dosed bags being recovered in the reticulorumen at slaughter. Ruminal degradation data obtained for tested forages were in accordance with those expected from the conventional degradability technique using fistulated animals, with no significant differences in the asymptotic values of degradation curves between bag shape or anti-regurgitation device. The results of this research demonstrate the potential for using a small bag technique with intact sheep to characterise the in situ ruminal degradability of roughages.     

树叶凋落物在受酸性矿山废水污染溪流中的分解

为了解华南地区酸性矿山废水对溪流中树叶分解的影响,在广东省大宝山矿区附近的1条受酸性矿山废水污染（pH值为2．7—3．4且富含多种重金属元素）的3级溪流中,利用2种孔径（5ram的网袋和0．1ram的布袋）的分解网袋对2种树叶（人面子和蒲桃）进行了为期101d的树叶分解研究。结果表明,人面子树叶网袋和布袋中的树叶干重剩余率分别为39％和48％,而蒲桃树叶网袋和布袋中的干重剩余率仍保持较高的水平,分别为61％和70％。根据指数衰减模型计算出树叶分解的半衰期,人面子树叶在网袋和布袋中的分解半衰期分别为57d和69d,而蒲桃树叶则分别为14-4d和217d。蒲桃树叶的分解速率明显比人面子树叶慢。在网袋中定殖的底栖动物主要是集食者,其中优势类群为摇蚊幼虫,占底栖动物个体总数的99％。摇蚊种群在网袋中的数量波动对2种树叶分解速率的影响并不明显。结果表明,受酸性矿山废水的影响,底栖动物群落的多样性大为减少。同时由于各种金属氧化物在树叶表面的不断沉淀,使树叶处于缺氧状态,抑制了微生物的活动,导致树叶分解速率大为降低。     

Validation of in sacco method: influence of sampling site,nylon bag or rumen contents,on fibrolytic activity of solid-associated microorganisms

Three ruminally cannulated cows, fed twice daily with a 70:30 forage:concentrate diet, were used to investigate the differences in fibrolytic activity of solid-associated microorganisms between nylon bags and rumen contents. Two different grass hays (regrowth and late harvested) were incubated in ruminal nylon bags. After 2 h or 23 h incubation time, pH was measured in bags and rumen contents, and enzymes of solid-associated microorganisms were extracted from bag residues and surrounding digesta by grinding, freezing, defrosting and sonication. Xylanase, avicelase, β-D-xylosidase and β-D-glucosidase activities were measured. Activities were lower in bag residues than in rumen digesta, and differences were greater after 2 h than after 23 h incubation time. Causes of these differences are discussed. For each incubation time and each enzyme, the differences in solid-associated microorganisms activities between rumen and bags contents were independent of the quality of hay in the bag. Thus the lower fibrolytic activity inside the bags may account for an underestimation of in vivo ruminal fiber degradation by the in sacco method, but this underestimation may be similar whatever the nature and content of forage cell walls.     

套袋对凤梨释迦袋内温光环境和果实品质的影响

探讨凤梨释迦Annona×atemoya套袋果实发育过程中不同类型果袋袋内温度、光参数变化,以及果袋类型对果实品质的影响。结果表明,不同类型果袋内温光环境差异大,果袋类型对果实外观及固形物含量存在显著影响。内黑外黄单层袋不透光,夜间袋温较高,果实固形物含量高于白色袋及半边透明白色袋,但果色较差,呈黄白色。白色袋具有一定透光性,白天袋内温度高于内黑外黄单层袋,果实呈浅绿色,果色较好。半边透明白色袋,袋内光强最大,其果实颜色、固形物含量与白色袋未见明显差异。不同类型果袋对果实大小,单果重,以及果柄的长度和粗度均未见显著影响。     

A modified litter bag design for use in lentic habitats

A modified litter bag design and handling procedure were tested to establish whether these reduced the exaggerated fragmentation losses that occur with standard litter bags. The modified design was compared with standard coarse (5 mm) and fine (0.25 mm) mesh litter bags using Phragmitesleaf litter. All were positioned in a section of a reedbed subject to water level management but negligible water flow. Breakdown rates were significantly reduced with the modified design but these were still significantly greater than those in the fine mesh bags. Owing to the extended period, results were influenced by invertebrates colonising the fine mesh bags. The significance of bag design and invertebrate colonisation are discussed.     

Complete nationwide survey on umbilical cord blood freezing bag breakage in Japan

Background aimsAlthough umbilical cord blood (UCB) has now become a common stem cell source, UCB bag breakage is a known risk in UCB transplantation (UCBT). This survey provides the first comprehensive data on the frequency and causes of UCB bag breakage in Japan.MethodsData regarding UCB bag breakage from all causes, identified between April 1, 2010, and September 3, 2013, were collected from all transplant centers registered for UCBT (209 hospitals) and all public cord blood banks (CBBs) (8 CBBs) in Japan.ResultsSeventeen incidents of UCB bag breakage at CBBs were confirmed, none of which resulted in bags being shipped to transplant centers. From among 3836 UCBT, 16 incidents (0.4%) of UCB bag breakage were confirmed at transplant centers. Although all these bags were used for transplantation, no direct health hazard was reported. The major cause of UCB bag breakage confirmed at transplant centers was considered to be external force (75%). In addition, 11 incidents of unexplained UCB bag breakage at sealing between compartments were reported.ConclusionsUCB bag breakage was confirmed at both CBBs and transplant centers. UCB bags should be handled with particular care and attention.     

Use of the mobile nylon bag technique to determine the digestible energy content of traditional and non-traditional feeds for swine

The following experiment was conducted using the mobile nylon bag technique (MNBT) to determine dry matter and energy digestibility in traditional feeds as well as non-traditional feeds in order to calculate digestible energy (DE) values for use in ration formulation programmes. A total of 22 ingredients were tested in this experiment including the traditional cereal grains barley, corn, oats and wheat, as well as secondary cereal grains such as normal and low viscosity rye, low lignin and high fat oat groats, wheat heavies and # 1 wheat screenings and a new bread wheat designated as CDC Teal. Three legumes (lupines, peas and dehydrated alfalfa), three varieties of canary seed (CDC Maria, dehulled CDC Maria and Keet), and raw and micronized canola seed were tested as protein sources. Finally, three oilseed meals obtained from the Chinese feed industry (Chinese rapeseed meal, Chinese cottonseed meal and extruded full-fat soybean meal) were also included. After simulating gastric digestion the nylon bags were inserted into the duodenum of five barrows through simple duodenal T-cannulae. Eight bags were administered to each pig daily. Ten nylon bags were prepared for each feed with two bags being inserted into each of the five barrows. A total of 220 bags were inserted over a 7-day period. The overall results of this experiment indicate that the MNBT has great potential for use in determining the digestible energy content of swine feeds. For the most part, values obtained in the present experiment compared favourably with previously published values. Four feedstuffs produced digestible energy values that differed by greater than 5% from previously reported values. However, variation in chemical content provided a reasonable explanation for these discrepancies. The MNBT has several advantages compared with conventional digestibility methods in that many feeds can be tested in a relatively short duration of time with significantly fewer animals being used, only small amounts of feed are required and the test allows for energy measurements in feedstuffs that would not normally be fed to pigs as a single ingredient. Additional research on a wider variety of feedstuffs should be conducted to confirm the potential of the MNBT as a tool to accurately determine DE values for swine.     

Assessing hunters’ ability to identify shot geese: implications for hunting bag accuracy

Reliable hunting bag statistics are a prerequisite for sustainable harvest management. Recently, Internet-based hunting bag reporting systems have been introduced in some European countries, e.g. Denmark, which may enable faster and more detailed reporting. However, reporting of waterfowl bags on a species-specific level may be biased from the individual hunters’ ability to correctly identify species, particularly because juvenile birds can only be identified from subtle differences. We assessed hunters’ ability to identify the five goose species huntable in Denmark. Identifications were made from a line-up of ten full-bodied geese including adults and juveniles. From a total of 2160 identifications made by active hunters, 85.5% were correct while 14.5% were assigned to a wrong species. Active hunters had on average an identification accuracy of 76.0%, highest for Canada goose (99.1%) and lowest for white-fronted goose (74.6%) and bean goose (73.7%). Identification accuracy was significantly lower for juvenile than for adult individuals of white-fronted and bean geese. Correcting the official Danish Bag Record (2013/2014) for identification accuracy, the bags of white-fronted and bean geese increase by 56.5 and 104.4%, respectively, while the bags of greylag and pink-footed geese decrease by 6.7 and 9.0%; the bag for Canada goose remains unchanged. Although identification accuracy is probably higher under field conditions, the study documents that inaccurate species identification is a source of bias in national bag statistics. Hence, improving identification skills by hunters is important to improve bag data accuracy when based on Internet reporting.     

The changing status of the Brown hare (Lepus capensis L.) in Britain

Game bag records are used to examine temporal and geographical changes in numbers of Brown hares. Records are taken from a survey made by the Oxford Bureau of Animal Population in 1938, which covered over 400 estates, and from the Game Conservancy's National Game Census, which has been monitoring shooting records of between 400 and 500 estates since 1961. Eastern regions of Britain had higher bags of hares than western regions in the three periods; 1891–1910, 1917–1936, 1961–1978. Hare game bags were generally high in counties with large proportions of tilled land. There was no consistent temporal trend in bags from different areas before 1938, but all areas show a considerable reduction in bags since 1961. This reduction is statistically significant. Time-series analysis of one extended set of data did not show evidence of cyclic fluctuations.     

Uncertainty and Sensitivity in the Carbon Footprint of Shopping Bags

Carbon footprints for several shopping bag alternatives (polyethylene, paper, cotton, biodegradable modified starch, and recycled polyethylene) were compared with life cycle assessment. Stochastic uncertainty analysis was used to study the sensitivity of the comparison to scenario and parameter uncertainty. On the basis of the results, we could give only a few robust conclusions without choosing a waste treatment scenario or limiting the parameter space. Given the scenario of current waste infrastructure in Finland, recycled polyethylene bags seem to be the most preferable (?7 to 24 g CO₂ eq./bag) and biodegradable bags the least preferable (38 to 60 g CO₂ eq./bag) option. In each analyzed waste treatment scenario, a few parameters dominated the uncertainty of results. Most of these parameters were downstream of the shopping bag manufacturing (consumer behavior, landfill conditions, method of waste combustion, etc.). The choice of waste treatment scenario had a greater effect on the ranking of bags than parameter uncertainty within scenarios. This result highlights the importance of including several scenarios in comparative life cycle assessments.     

Storage of apheresis platelets in ethylene-vinyl acetate copolymer bags: relationship between the bag size and the number of platelets maintaining aerobic metabolism

The present study was designed to determine whether a bag made from ethylene-vinyl acetate copolymer (EV) with superior flexibility at subzero temperature is suitable for a storage container of single-donor apheresis platelets. Apheresis platelets were stored with 100 ml plasma in 1-liter bags made of EV or standard polyvinyl chloride (PVC) plastic at 22 degrees C with constant agitation. The oxygen permeability of the 1-liter EV bag averaged 1447 nmol/min/atm, which was about 1.5 times higher than that of PVC bags. The partial oxygen tension (PO2) of platelet concentrates (PC) has linearly decreased to 16 mm Hg with increasing platelet counts. The level of the partial carbon dioxide was always higher in EV bags than in PVC bags. Oxygen consumption rates of platelets stored in EV and PVC bags with a sufficient oxygen supply averaged 1.25 and 1.20 nmol/min/10(9) platelets, respectively. The rates of glucose consumption and lactate production were not changed in two bags. Ninety percent of the total ATP production of about 8 nmol/min/10(9) platelets were generated through the aerobic metabolism. The platelet counts in the 1-liter EV and PVC bags, at which PO2 is 16 mm Hg, were 2.2 and 1.5 x 10(11) platelets, respectively. The study indicates that apheresis platelets stored in EV bags at 22 degrees C have no different metabolic changes when compared with those of PVC bags. In addition, the number of platelets maintaining the aerobic metabolism is 1.5 times higher than that of PVC bags.     

Effects of water-stress on the fungal growth and quality of vegetable amaranth in storage

Amaranthus crucentus are usually short lived annual crops which probably originated in the Andcan region of South America. An experiment was conducted to determine the effects of water stress on the microbial growth and quality of vegetable Amaranth. Analysis of variance showed that microbial growth was significant at 17.63% as well as moisture stress at 128.1%. However nitrogen composition and water content was not significant at the 5% probability level. A polyethylene bag recorded the highest microbial growth 38.33% in comparison with a paper bag 14.67%, when those exposed had the lowest microbial growth 7.33%. Amaranthus crucentus packaged with polythene bags recorded the highest microbial growth, followed by those packaged with paper bags while those exposed had the lowest. The microbes observed were mainly Aspergillus species. The result of the investigation showed that on the first day, vegetable Amaranth packaged with paper bag had the highest weight loss of 17.02 g in comparison with the exposed sample of 14.08 g, while those packaged with polyethylene bags had the lowest weight loss of 0.33. Similar trends were observed on the third and sixth days respectively. Investigation showed that the exposed samples recorded the highest nitrogen composition of 0.06% followed by paper bag 0.030%, while polythene had the lowest 0.028%. However, vegetables packaged with polythene bags had the highest water content, followed by paper bag 62.25%, while the exposed recorded the lowest water content of 43.96%.