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酵母PHO2与PHO4蛋白的激活活性的分析及两者的相互作用 总被引:3,自引:3,他引:0
PHO2与PHO4是酵母PHO5基因的两个正调控因子,本文发现,PHO2与酵母转录因子GAL4的DNA结合功能域融合后就能激活报道基因lacZ的表达,其激活力受高低磷影响,表明PHO2蛋白上存在酸性转录激活区。PHO2蛋白上酸性氨基酸丰富的287-326肽段并非PHO2的激活区。在PHO2蛋白上230位Ser处于磷酸化状态2PHO2才有激活作用,表明了这一磷酸化位点可能与PHO2的转录激活能力有关 相似文献
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Duan R Xie W Li X McDougal A Safe S 《Biochemical and biophysical research communications》2002,294(2):384-394
17Beta-estradiol (E2) induces proliferation and c-fos gene expression in MCF-7 cells and both responses are partially blocked by wortmannin and LY294002 which are inhibitors of phosphatidylinositol-3-kinase (PI3-K). Analysis of the c-fos gene promoter shows that the effects of wortmannin and LY294002 are associated with inhibition of E2-induced activation through the serum response factor (SRF) motif within the proximal serum response element at -325 and -296. E2 activates constructs containing multiple copies of the SRF (pSRF) and a GAL4-SRF fusion protein; these responses are accompanied by PI3-K-dependent phosphorylation of Akt and inhibited by wortmannin/LY294002, the antiestrogen ICI 182780, but not by the mitogen-activated protein kinase kinase (MAPKK) inhibitor PD98059. Using a series of kinase inhibitors and dominant negative kinase expression plasmids, it was shown that the non-genomic activation of SRF by E2 was associated with src-ras-PI3-K pathway, thus, demonstrating hormonal activation of the SRE through src-ras activation of both PI3-K- and MAPK-dependent signaling pathways. 相似文献
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The human transcriptional repressor protein NAB1: expression and biological activity 总被引:4,自引:0,他引:4
Thiel G Kaufmann K Magin A Lietz M Bach K Cramer M 《Biochimica et biophysica acta》2000,1493(3):289-301
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