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1.
Xanne Janssen Dylan P. Cliff John J. Reilly Trina Hinkley Rachel A. Jones Marijka Batterham Ulf Ekelund S?ren Brage Anthony D. Okely 《PloS one》2013,8(11)
Objectives
Evaluate the predictive validity of ActiGraph energy expenditure equations and the classification accuracy of physical activity intensity cut-points in preschoolers.Methods
Forty children aged 4–6 years (5.3±1.0 years) completed a ∼150-min room calorimeter protocol involving age-appropriate sedentary, light and moderate-to vigorous-intensity physical activities. Children wore an ActiGraph GT3X on the right mid-axillary line of the hip. Energy expenditure measured by room calorimetry and physical activity intensity classified using direct observation were the criterion methods. Energy expenditure was predicted using Pate and Puyau equations. Physical activity intensity was classified using Evenson, Sirard, Van Cauwenberghe, Pate, Puyau, and Reilly, ActiGraph cut-points.Results
The Pate equation significantly overestimated VO2 during sedentary behaviors, light physical activities and total VO2 (P<0.001). No difference was found between measured and predicted VO2 during moderate-to vigorous-intensity physical activities (P = 0.072). The Puyau equation significantly underestimated activity energy expenditure during moderate-to vigorous-intensity physical activities, light-intensity physical activities and total activity energy expenditure (P<0.0125). However, no overestimation of activity energy expenditure during sedentary behavior was found. The Evenson cut-point demonstrated significantly higher accuracy for classifying sedentary behaviors and light-intensity physical activities than others. Classification accuracy for moderate-to vigorous-intensity physical activities was significantly higher for Pate than others.Conclusion
Available ActiGraph equations do not provide accurate estimates of energy expenditure across physical activity intensities in preschoolers. Cut-points of ≤25counts⋅15 s−1 and ≥420 counts⋅15 s−1 for classifying sedentary behaviors and moderate-to vigorous-intensity physical activities, respectively, are recommended. 相似文献2.
Lifetime of the excited state (τ) of bacteriochlorophyll (BChl) in photosynthetic bacteria, measured with a mode-locked argon laser (oscillating at 488 nm; mode locked at 56 MHz) as light source, ranged from 0.3 to 2.5 nsec. These τ values are reported with a precision of ±0.1 nsec. The value of τ at high exciting light intensity (I) was two to three times that at low intensity. For young cultures of green bacterium Chloropseudomonas ethylicum, τ ranged from 0.5 (low I) to 1.0 nsec (high I); for those of the purple bacterium Rhodospirillum rubrum, from 0.4 (low I) to 1.0 nsec (high I); and for those of the BChl b-containing Rhodopseudomonas viridis, from 1.0 (low I) to 2.5 nsec (high I). These data provide information regarding the efficiencies of the photochemical process in these bacteria. Quantum yield (ø) of BChl fluorescence, calculated from ø = τ/τ0 (where τ0 is the intrinsic lifetime of fluorescence), ranges from 2-6% at low intensities to 6-14% at high intensities. 相似文献
3.
Ching-Kang Chen Michael L. Woodruff Gordon L. Fain 《The Journal of general physiology》2015,145(3):213-224
Light stimulates rhodopsin in a retinal rod to activate the G protein transducin, which binds to phosphodiesterase (PDE), relieving PDE inhibition and decreasing guanosine 3′,5′-cyclic monophosphate (cGMP) concentration. The decrease in cGMP closes outer segment channels, producing the rod electrical response. Prolonged exposure to light decreases sensitivity and accelerates response kinetics in a process known as light adaptation, mediated at least in part by a decrease in outer segment Ca2+. Recent evidence indicates that one of the mechanisms of adaptation in mammalian rods is down-regulation of PDE. To investigate the effect of light and a possible role of rhodopsin kinase (G protein–coupled receptor kinase 1 [GRK1]) and the GRK1-regulating protein recoverin on PDE modulation, we used transgenic mice with decreased expression of GTPase-accelerating proteins (GAPs) and, consequently, a less rapid decay of the light response. This slowed decay made the effects of genetic manipulation of GRK1 and recoverin easier to observe and interpret. We monitored the decay of the light response and of light-activated PDE by measuring the exponential response decay time (τREC) and the limiting time constant (τD), the latter of which directly reflects light-activated PDE decay under the conditions of our experiments. We found that, in GAP-underexpressing rods, steady background light decreased both τREC and τD, and the decrease in τD was nearly linear with the decrease in amplitude of the outer segment current. Background light had little effect on τREC or τD if the gene for recoverin was deleted. Moreover, in GAP-underexpressing rods, increased GRK1 expression or deletion of recoverin produced large and highly significant accelerations of τREC and τD. The simplest explanation of our results is that Ca2+-dependent regulation of GRK1 by recoverin modulates the decay of light-activated PDE, and that this modulation is responsible for acceleration of response decay and the increase in temporal resolution of rods in background light. 相似文献
4.
The filamentous cyanobacterium Microcoleus vaginatus, a major primary producer in desert biological sand crusts, is exposed to frequent hydration (by early morning dew) followed by desiccation during potentially damaging excess light conditions. Nevertheless, its photosynthetic machinery is hardly affected by high light, unlike “model” organisms whereby light-induced oxidative stress leads to photoinactivation of the oxygen-evolving photosystem II (PSII). Field experiments showed a dramatic decline in the fluorescence yield with rising light intensity in both drying and artificially maintained wet plots. Laboratory experiments showed that, contrary to “model” organisms, photosynthesis persists in Microcoleus sp. even at light intensities 2–3 times higher than required to saturate oxygen evolution. This is despite an extensive loss (85–90%) of variable fluorescence and thermoluminescence, representing radiative PSII charge recombination that promotes the generation of damaging singlet oxygen. Light induced loss of variable fluorescence is not inhibited by the electron transfer inhibitors 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU), 2,5-dibromo-3-methyl-6-isopropylbenzoquinone (DBMIB), nor the uncoupler carbonyl cyanide-p-trifluoromethoxyphenylhydrazone (FCCP), thus indicating that reduction of plastoquinone or O2, or lumen acidification essential for non-photochemical quenching (NPQ) are not involved. The rate of QA
− re-oxidation in the presence of DCMU is enhanced with time and intensity of illumination. The difference in temperatures required for maximal thermoluminescence emissions from S2/QA
− (Q band, 22°C) and S2,3/QB
− (B band, 25°C) charge recombinations is considerably smaller in Microcoleus as compared to “model” photosynthetic organisms, thus indicating a significant alteration of the S2/QA
− redox potential. We propose that enhancement of non-radiative charge recombination with rising light intensity may reduce harmful radiative recombination events thereby lowering 1O2 generation and oxidative photodamage under excess illumination. This effective photo-protective mechanism was apparently lost during the evolution from the ancestor cyanobacteria to the higher plant chloroplast. 相似文献
5.
Replication-competent viruses have been used as an alternative therapeutic approach for cancer treatment. However, new clinical data revealed an innate immune response to virus that may mitigate the effects of treatment. Recently, Wein, Wu and Kirn have established a model which describes the interaction between tumor cells, a replication-competent virus and an immune response (Cancer Research 63 (2003):1317–1324). The purpose of this paper is to extend their model from the viewpoints of mathematics and biology and then prove global existence and uniqueness of solution to this new model, to study the dynamics of this novel therapy for cancers, and to explore a explicit threshold of the intensity of the immune response for controlling the tumor. We also study a time-delayed version of the model. We analytically prove that there exists a critical value τ0 of the time-delay τ such that the system has a periodic solution if τ>τ0. Numerical simulations are given to verify the analytical results. Furthermore, we numerically study the spatio-temporal dynamics of the model. The effects of the diffusivity of the immune response on the tumor growth are also discussed.Mathematics Subject Classification (2000): 35R35, 92A15 相似文献
6.
H. Keffer Hartline 《The Journal of general physiology》1923,6(2):137-152
1. The negative phototropism of certain land isopods was investigated over a large range of intensities, especially low ones. The responses were determined quantitatively by measuring the angle through which an animal turned away from a line perpendicular to the rays of light. 2. In the absence of light the undirected movements set up by obscure stimuli were such as to compensate each other statistically, the average path being a movement in the direction in which the animal was headed. 3. Over a large range of intensities (0.0026 m.c. up) the average turning is maximal, about 55° (Oniscus). This maximal response is due to an anatomical peculiarity, in that the carapace cuts off the light on the eye after the animal has turned 50–60°. This peculiarity probably accounts for specific differences among land isopods. Any light, therefore, which is strong enough to turn an animal through this maximal angle in a radial distance of 10 cm. will give results whose mean will be maximal. 4. Below 0.0026 m.c. the amount of angular deflection becomes less and less, in proportion to the logarithm of the intensity, until at 0.00003 m.c. the movements are the same as in darkness. 5. This proportionality between amount of turning and the logarithm of the intensity indicates the photochemical nature of phototropism on the basis of Hecht''s work with Mya. As a result, Loeb''s theory of phototropism may then be stated in the mathematical form See PDF for Equation in which I
1 and I
2 are the two intensities, E
1 and E
2, their respective effects, and R, the muscular action set up by the difference in photochemical effect on the two sides. 相似文献
7.
Paul R. Dohrmann Raul Correa Ryan L. Frisch Susan M. Rosenberg Charles S. McHenry 《Nucleic acids research》2016,44(3):1285-1297
There is widespread agreement that the clamp loader of the Escherichia coli replicase has the composition DnaX3δδ’χψ. Two DnaX proteins exist in E. coli, full length τ and a truncated γ that is created by ribosomal frameshifting. τ binds DNA polymerase III tightly; γ does not. There is a controversy as to whether or not DNA polymerase III holoenzyme (Pol III HE) contains γ. A three-τ form of Pol III HE would contain three Pol IIIs. Proponents of the three-τ hypothesis have claimed that γ found in Pol III HE might be a proteolysis product of τ. To resolve this controversy, we constructed a strain that expressed only τ from a mutated chromosomal dnaX. γ containing a C-terminal biotinylation tag (γ-Ctag) was provided in trans at physiological levels from a plasmid. A 2000-fold purification of Pol III* (all Pol III HE subunits except β) from this strain contained one molecule of γ-Ctag per Pol III* assembly, indicating that the dominant form of Pol III* in cells is Pol III2τ2 γδδ’χψ. Revealing a role for γ in cells, mutants that express only τ display sensitivity to ultraviolet light and reduction in DNA Pol IV-dependent mutagenesis associated with double-strand-break repair, and impaired maintenance of an F’ episome. 相似文献
8.
John Pooler 《The Journal of general physiology》1972,60(4):367-387
Photodynamic alteration of lobster giant axons drastically changed the magnitude and kinetics of sodium currents seen under voltage clamp using the sucrose gap technique. Illumination of axons following treatment with acridine orange or eosin Y decreased the maximum sodium conductance to a zero asymptote as an exponential function of illumination time. Normal sodium inactivation was slowed, with τh more than doubled depending on experimental conditions. A second slower inactivation rate developed occasionally. τh was altered little, if at all. Sodium current "tails" were not prolonged. At maximum light intensity and with eosin Y as sensitizer leakage current increased after 4–10 sec in light. These changes were irreversible. Decreases in maximum sodium conductance correlated highly with increases in time to peak sodium current. The magnitude of change varied linearly with light intensity. The action spectra for eosin Y and acridine orange peaked near 545 and 505 nm, respectively. The magnitude of change varied with preillumination dye exposure time in a quasi-exponential approach to a maximum effect. Sodium dithionite protected the axon from photodynamic change. 相似文献
9.
Using synchrotron radiation as the excitation light, we studied the fluorescence parameters of perylene incubated with pigeon erythrocyte membranes and with an isotropic viscous medium, the Primol 342 oil.From 4 to 37°C, we observed a single lifetime of 4.5 ns in the oil and two with the membrane (τ1 = 1−1.4 ns and τ2 = 5.4−6.1 ns). The dependence upon temperature of the rotation correlation time of perylene () in the oil was characteristic of an isotropic medium, whereas the limiting value of anitropy (r ∞) was zero. With the membrane, γ ∞ decreased from 0.14 to 0.06 and from 2.9 to 0.5 ns, indicating a greater amplitude and frequency of molecular motions.The addition of chlorpromazine, indomethacine, tetracaine, n-octylamine, octanol or octanoic acid to the membrane decreased the τ1 and τ2 values. This would stem from the desorganization of the membrane induced by the drugs. 相似文献
10.
11.
Ecological Consequences of Long-Term Exposure of Anabaena variabilis (Cyanophyceae) to Shifts in Environmental Factors 总被引:2,自引:0,他引:2
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Cultures of Anabaena variabilis were exposed to different light intensities, and the time course of photoadaptation was measured by photosynthetic rate and changes in pigmentation. A shift down in intensity of 284 μEin · m−2 · sec−1 caused a temporary decrease in the photosynthetic response followed by gradual adaptation to the new conditions. Final chlorophyll a and carotenoid concentrations were reached after 1 day, although other physiological indicators showed that adaptation required 4 days. The parameter Ik was shown to be the best indicator of photoadaptation. A shift up in light intensity of the same magnitude also required 4 days for complete photoadaptation by the culture, although chlorophyll and carotenoid concentrations stabilized within 1 day. A shift down in light intensity of 392 μEin · m−2 · sec−1 resulted in a temporary attempt to adapt followed by collapse of the population. This demonstrates an apparent threshold in the magnitude of the shift in light intensity which will permit successful adaptation. Simultaneous changes in light intensity and temperature also adversely affected culture populations. Our observations present a possible cause for the decline or prevention of an algal bloom under a fluctuating light regime and suggest that it may be possible to predict this decline as a result of synoptic weather patterns or hydrodynamic influences. 相似文献
12.
1. Green plants have been found to emit light of approximately the same color as their fluorescent light for several minutes following illumination. This light is about 10–3 the intensity of the fluorescent light, about one-tenth second after illumination below saturation or 10–6 of the intensity of the absorbed light. 2. The decay curve follows bimolecular kinetics at 6.5°C. and reaction order 1.6 at 28°C. 3. This light saturates as does photosynthesis at higher light intensities and in about the same intensity range as does photosynthesis. 4. An action spectrum for light emitted as a function of the wave length of exciting light has been determined. It parallels closely the photosynthetic action spectrum. 5. The intensity of light emission was studied as a function of temperature and found to be optimal at about 37°C. with an activation energy of approximately 19,500 calories. Two-temperature studies indicated that the energy may be trapped in the cold, but that temperatures characteristic for enzymatic reactions are necessary for light production. 6. Illumination after varying dark periods showed initial peaks of varying height depending on the preceding dark period. 7. 5 per cent CO2 reversibly depresses the amount of light emitted by about 30 per cent. About 3 minutes are required for this effect to reach completion at room temperatures. 8. Various inhibitors of photosynthesis were tested for their effect on luminescence and were all inhibitory at appropriate concentrations. 9. Irradiation with ultraviolet light (2537A) inhibits light production at about the same rate as it inhibits photosynthesis. 10. This evidence suggests that early and perhaps later chemical reactions in photosynthesis may be partially reversible. 相似文献
13.
Ramma Sawhney 《Planta》1977,133(2):103-106
Seedlings of C. rubrum were irradiated with different light qualities and intensities following a single inductive dark period. Our results show that relatively low intensity white light (35–100 ft. c.) does not support flower development while high intensity white light (650–800 ft. c.) permits 100% flowering. We have shown that the low intensity light inhibiton of flower development is not due to suboptimal photosynthesis. Relatively low intensities of light rich in far-red or blue wavebands sustains optimum flower development, whereas red light is totally ineffective in this respect. Considering that the intensity dependent High Energy Reaction (HER) has its action maxima in the blue and far-red we propose that HER may be positively involved in the flower development of C. rubrum. Our study further suggests that there may be some flower inhibitory component at play in relatively low intensity white light conditions and HER may be required to counteract this flower inhibitory effect.Abbreviations SD
short day plant
- HER
High Energy Reaction
- PFR
far-red absorbing form of phytochrome
- PR
red absorbing form of phytochrome
- L.I.I.
low intensity incandescent white light
- H.I.I.
high intensity incandescent white light
- L.I.F.
low intensity fluorescent white light
- H.I.F.
high intensity fluorescent white light
- GA3
gibbrellic acid
This paper constitutes a part of a Ph.D. thesis submitted to the University of Western Ontario, London, Ontario. 相似文献
14.
C(3)-C(4) Intermediate Species in Alternanthera (Amaranthaceae) : Leaf Anatomy, CO(2) Compensation Point, Net CO(2) Exchange and Activities of Photosynthetic Enzymes
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Two naturally occurring species of the genus Alternanthera, namely A. ficoides and A. tenella, were identified as C3-C4 intermediates based on leaf anatomy, photosynthetic CO2 compensation point (Γ), O2 response of г, light intensity response of г, and the activities of key enzymes of photosynthesis. A. ficoides and A. tenella exhibited a less distinct Kranz-like leaf anatomy with substantial accumulation of starch both in mesophyll and bundle sheath cells. Photosynthetic CO2 compensation points of these two intermediate species at 29°C were much lower than in C3 plants and ranged from 18 to 22 microliters per liter. Although A. ficoides and A. tenella exhibited similar intermediacy in г, the apparent photorespiratory component of O2 inhibition in A. ficoides is lower than in A. tenella. The г progressively decreases from 35 microliters per liter at lowest light intensity to 18 microliters per liter at highest light intensity in A. tenella. It was, however, constant in A. ficoides at 20 to 25 microliters per liter between light intensities measured. The rates of net photosynthesis at 21% O2 and 29°C by A. ficoides and A. tenella were 25 to 28 milligrams CO2 per square decimeter per hour which are intermediate between values obtained for Tridax procumbens and A. pungens, C3 and C4 species, respectively. The activities of key enzymes of C4 photosynthesis, phosphoenolpyruvate carboxylase, pyruvate Pi dikinase, NAD malic enzyme, NADP malic enzyme and phosphoenolpyruvate carboxykinase in the two intermediates, A. ficoides and A. tenella are very low or insignificant. Results indicated that the relatively low apparent photorespiratory component in these two species is presumably the basis for the C3-C4 intermediate photosynthesis. 相似文献
15.
Mary Mitchell Moore 《The Journal of general physiology》1927,8(6):509-518
1. When Cerianthus membranaceus is illuminated upon one side, the animal turns its anterior portion toward the source of light. The number of degrees through which the animal turns is proportional to the logarithm of the intensity of the light. 2. A light intensity of between 250 m.c. and 15,000 m.c. is necessary to cause retraction of the animal. 3. The part of the spectrum which is most effective in causing heliotropic bending of Cerianthus lies between µµ 510 and µµ 570. 相似文献
16.
X.A. lvarez-Salgado U. Labarta M.J. Fernndez-Reiriz F.G. Figueiras G. Rosn S. Piedracoba R. Filgueira J.M. Cabanas 《Harmful algae》2008,7(6):849-855
The extension and intensity of the upwelling season in the NW Iberian Peninsula (42°N–43°N) have decreased by 30 and 45% over the last 40 years, respectively. Accordingly, the renewal time (τ) of the Rías Baixas, four large coastal inlets where 15% of the World extraction of blue mussels occurs, has increased by 240%. We indirectly demonstrate here that the growing τ has caused the increasing occurrence of harmful microalgae in these embayments, dramatically affecting mussel raft cultivation. The equation D = 365(1 − exp(−τ/c1)) explains 80% of the variability of the number of days per year that mussels cannot be extracted from the hanging ropes because of the occurrence of harmful microalgae (D). The coefficient c1 = 37 ± 2 days indicates that an average τ over the upwelling season of >25 ± 1 or 50 ± 3 days reduce mussel extraction to only 50 or 25% of the year, respectively. 相似文献
17.
CROZIER WJ 《The Journal of general physiology》1950,34(1):87-136
1. Seeing-frequency functions (ψ[S]) determined uniocularly for small (1.6'), brief images at the thoroughly dark-adapted human central fovea take the form of log-Gaussian integrals, for intensity or for exposure-time as the independent variable. They cannot be of Poisson type, since mean (τ') and standard deviation (σ) are quite independently modifiable. 2. The properties of τ', the abscissa of inflection in the log-Gaussian integrals, and of σ, are discussed in relation to customary determinations of spectral visibilities; agreements and certain necessary divergences are considered. 3. The values of σlogΔIlogΔI0, are distributed periodically, with 5 maxima in the range λ383 to λ712. Outside the fovea this is not the case. These maxima, and the intervening minima, are definitely correlated with "irregularities" in the values of τ' as function of λ, with data on color threshold intensities, with data on "saturation," and quite specifically with minima and maxima in the data of λ discrimination. They are unmistakably of analytical significance. 4. The interpretation of these properties is taken to require (consistent with the indications of other evidence) that under specified conditions the magnitude of σ is a measure of the "size" of the population of excitable elements of neural effect, in the range of 0 to 100 per cent response-frequency as a function of wave number. This means that under the procedure used there is detectable the spectral distribution of local, specific, photosensitization. The σ's refer to a property of the excitation process which is not affected by the action of irrelevant absorbers. 5. A preliminary test of this has been made by the use of mixed "monochromatic" lights. The total energy is empirically the significant quantity, although the separate effects of λ's are sharply exhibited. 6. The λ distribution of photosensitization indices, σlogΔIlogΔI0, is necessarily taken to have the nature of "action spectra." Careful search discloses, surprisingly enough, precise and apparently unique correspondence with the maxima and minima (and details of shape) required for the participation of: cytochrome-c reductase; the reduced forms of cytochromes-a and -c; and the often overlooked ferric-prophyrin bands of oxidized cytochrome-c. 7. For the central fovea, in which blood vessels are absent, and in which resistance to anoxia is at a maximum, the presence of an enzyme system of the sort here indicated is entirely reasonable. It is pointed out that a number of specific experiments can be based upon the general finding, whatever the causal mechanism for the sensitized λ regions may in the end prove to be. The data cannot be reconciled to the demands of existing color primary theories, although apparently quite accurately consistent with varied observational data. 相似文献
18.
Excitation-contraction coupling in a barnacle muscle fiber as examined with voltage clamp technique 总被引:9,自引:5,他引:4
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Relations between the membrane potential and the tension associated with changes in membrane potential were analyzed in barnacle giant muscle fibers by using voltage clamp techniques. With a step change in membrane potential the tension reaches its final level with a time course which is expressed by the difference of two exponential functions. The time constants τ1 (0.2–0.4 sec at 23°C) and τ2 (0.07–0.12 sec at 23°C) are independent of the new membrane potential at least for a relatively small membrane potential change while the final level of tension is a function of the potential. Decreasing the temperature increases both τ1 and τ2 (Q10 = -2 to -3) and the increase of the tonicity of the external medium increases τ1 but not τ2. The final level of tension is related by an S-shaped curve to the membrane potential. The slope of the final tension-membrane potential curve increases with increasing external Ca concentration and is reduced when a small amount of transition metal ions is added to the medium. This suggests that the influx of Ca ions through the membrane is an important factor in the development of tension. 相似文献
19.
Background and Aims
Recent studies have suggested that responses to shading gradients may play an important role in establishment success of exotic plants, but hitherto few studies have tested this. Therefore, a common-garden experiment was conducted using multiple Asian woody plant species that were introduced to Europe >100 years ago in order to test whether naturalized and non-naturalized species differ in their responses to shading. Specifically, a test was carried out to determine whether naturalized exotic woody species maintained better growth under shaded conditions, and whether they expressed greater (morphological and physiological) adaptive plasticity in response to shading, relative to non-naturalized species.Methods
Nineteen naturalized and 19 non-naturalized exotic woody species were grown under five light levels ranging from 100 to 7 % of ambient light. For all plants, growth performance (i.e. biomass), morphological and CO2 assimilation characteristics were measured. For the CO2 assimilation characteristics, CO2 assimilation rate was measured at 1200 μmol m–2 s–1 (i.e. saturated light intensity, A1200), 50 μmol m–2 s–1 (i.e. low light intensity, A50) and 0 μmol m–2 s–1 (A0, i.e. dark respiration).Key Results
Overall, the naturalized and non-naturalized species did not differ greatly in biomass production and measured morphological and CO2 assimilation characteristics across the light gradient. However, it was found that naturalized species grew taller and reduced total leaf area more than non-naturalized species in response to shading. It was also found that naturalized species were more capable of maintaining a high CO2 assimilation rate at low light intensity (A50) when grown under shading.Conclusions
The results indicate that there is no clear evidence that the naturalized species possess a superior response to shading over non-naturalized species, at least not at the early stage of their growth. However, the higher CO2 assimilation capacity of the naturalized species under low-light conditions might facilitate early growth and survival, and thereby ultimately favour their initial population establishment over the non-naturalized species. 相似文献20.
The solution structure of the C-terminal Domain V of the τ subunit of E. coli DNA polymerase III was determined by nuclear magnetic resonance (NMR) spectroscopy. The fold is unique to τ subunits. Amino acid sequence conservation is pronounced for hydrophobic residues that form the structural core of the protein, indicating that the fold is representative for τ subunits from a wide range of different bacteria. The interaction between the polymerase subunits τ and α was studied by NMR experiments where α was incubated with full-length C-terminal domain (τC16), and domains shortened at the C-terminus by 11 and 18 residues, respectively. The only interacting residues were found in the C-terminal 30-residue segment of τ, most of which is structurally disordered in free τC16. Since the N- and C-termini of the structured core of τC16 are located close to each other, this limits the possible distance between α and the pentameric δτ2γδ′ clamp–loader complex and, hence, between the two α subunits involved in leading- and lagging-strand DNA synthesis. Analysis of an N-terminally extended construct (τC22) showed that τC14 presents the only part of Domains IVa and V of τ which comprises a globular fold in the absence of other interaction partners. 相似文献