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1.
Introduction: The authors initiated the use of Liqui‐PREP? (LGM International Inc., Fort Lauderdale, FL, USA) in August, 2005. Cytotechnologists received extensive (one month) training by cytopathologists experienced in Liquid‐based cytology. The Liqui‐PREP? direct‐to‐vial procedure (LP) was compared to the conventional Pap smears in a routine screening population. Methods: Data derived from 26 178 LP cervical‐vaginal (CV) specimens were compared to data derived from 218 548 conventional Pap smears (CS). Both data sets reflect patient samples collected concurrently (August–December, 2005) by 117 participating outpatient medical practices from a well‐defined geographic area. There were no significant personnel changes during the study period. The diagnostic results, classified according to Bethesda criteria were calculated. Results:
Discussion: Liqui‐PREP? direct‐to‐vial method for CV specimens identified 210% more LSIL and 56% more HSIL+ lesions compared to the conventional smears. The ASCUS rate was increased (perhaps due to the conservative nature of our staff and their cautious interpretation of a new preparation). The ratio of ASCUS to LSIL+ was reduced by 5% for Liqui‐Prep?. Available biopsy data showed high correlation between both LP and CS abnormal cytology diagnoses (94.1% and 89.9% respectively). These findings suggest that the Liqui‐PREP? cytology preparation procedure identifies more pre‐malignant lesions than the conventional smear. 相似文献
% ASC‐US | % ASC‐H | % LSIL | % HSIL+ | ASCUS/ LSIL+ | % Unsat. | |
---|---|---|---|---|---|---|
Liqui‐PREP? | 6.5 | 0.24 | 1.55 | 0.39 | 3.8 | 0.02 |
Conv. Smear | 2.8 | 0.09 | 0.50 | 0.25 | 4.0 | 0.05 |
2.
Züleyha A. etinkaya Mesut Sezikli Fatih Güzelbulut Süleyman Cogun Serkan Düzgün Oya
. Kurda 《Helicobacter》2010,15(2):143-147
Aim: To compare the efficacy of 14‐day and 5‐day amoxicillin treatment on the eradication rate during tetracycline containing sequential H. pylori therapy, and also to compare the eradication rate of this regimen with those used in similar studies performed in Turkey. Method: This study included 112 patients infected with H. pylori that were randomized into 2 groups. In group A, patients (n = 56) received pantoprazole (40 mg BID) and amoxicillin (1 g BID) for 5 days, followed by pantoprazole (40 mg BID), tetracycline (500 mg QID), and metronidazole (500 mg TID) for the remaining 9 days. In group B, patients (n = 56) received pantoprazole (40 mg BID) and amoxicillin (1 g BID) for 5 days, followed by pantoprazole (40 mg BID), tetracycline (500 mg QID), metronidazole (500 mg TID), and amoxicillin (1 g BID) for the remaining 9 days. Eradication rates were calculated using both intention‐to‐treat (ITT) and per‐protocol (PP) analyses. Results: In all, 112 patients were subjected to ITT analysis and 109 patients completed the study. In group A, H. pylori eradication was achieved in 46 (82.1%) of the 56 patients included in the ITT analysis and in 46 (83.6%) of the 55 patients included in the PP analysis. In group B, H. pylori eradication was achieved in 44 (78.57%) of the 56 patients included in the ITT analysis and in 44 (81.48%) of the 54 patients included in the PP analysis ( Table 2 ). The eradication rates were not statistically significant between the 2 groups (p > .005). Table 2. Eradication rates in the two study groups
Group A | Group B | p | |||
---|---|---|---|---|---|
n | ITT/PP | n | ITT/PP | ||
Eradication | |||||
Female | 21 | 70%/72.4% | 34 | 79.06%/82.9% | NS |
Male | 25 | 6.1%/96.1% | 10 | 76.9%/76.9% | NS |
Total | 46 | 82.1%/83.6% | 44 | 78.57%/81.48% | NS |
- NS, not significant; PP, per‐protocol; ITT, intention‐to‐treat.
3.
D. N. Rana R. V. Persad M. Desai D. M. Perera H. El Teraifi J. Marshall 《Cytopathology》2003,14(Z1):5-5
Aims Table 1. . The outcome status of these women
Table 2 shows the outcome of women with borderline and mild dyskaryosis smears with or without koilocytosis. Table 2. The outcome of women with borderline and mild dyskaryosis smears with or without koilocytosis
Table 3 shows the proportion of borderline and mild dyskaryosis cervical smears with or without koilocytosis. Table 3. The proportion of borderline and mild dyskaryosis cervical smears with or without koilocytosis
Conclusions
- 1 To identify the outcome status of women with borderline and mild dyskaryosis smears.
- 2 To determine whether the presence or absence of koilocytosis influences the outcome status.
- 3 To identify the proportion of women with borderline smears showing koilocytosis.
Cytology | Outcome status | ||
---|---|---|---|
Negative (%) | Low‐grade (%) | High‐grade (%) | |
Borderline | 68 | 19 | 13 |
Mild dyskaryosis | 46 | 26 | 28 |
Koilocytosis | Outcome status | ||
---|---|---|---|
Negative (%) | Low‐grade (%) | High‐grade (%) | |
Present | 58 | 22 | 20 |
Absent | 61 | 21 | 18 |
Cytology | Koilocytosis present (%) | Koilocytosis absent (%) |
---|---|---|
Borderline | 24 | 76 |
Mild dyskaryosis | 34 | 66 |
- 1 Sixty‐eight per cent of women with a borderline cervical smear had a normal outcome.
- 2 Thirteen per cent of women with a borderline cervical smear developed a high‐grade lesion.
- 3 The presence or absence of koilocytosis in borderline and mild dyskaryosis cervical smears does not appear to affect the outcome status of these women.
- 4 Twenty‐four per cent of smears showing borderline nuclear changes were found to have koilocytosis.
4.
Andrew Evered 《Cytopathology》2003,14(Z1):14-14
Introduction Direct endometrial sampling with cytology and or histology is used at our hospital as part of the investigation of abnormal uterine bleeding. It is used in cases where there is a low clinical suspicion of malignancy. The advantage of the technique is that it can be done as an outpatient procedure with minimal patient discomfort. Reports in the literature give mixed results. We present a 3‐year retrospective of our experience with follow‐up.
Results Eighty‐eight cases were examined with an age range of 42–82. Review of the false negative case showed no malignant cells and is likely to represent a sampling problem. Conclusions
Result | Cytology | Biopsy | Follow‐up histology |
---|---|---|---|
Inadequate | 9 | 9 | One ovarian adenocarcinoma |
negative | 75 | 66 | One adenocarcinoma nine benign |
Suspicious | 3 | One hyperplasia | One hyperplasia one polyp |
Malignant | 1 | 1 | Adenocarcinoma |
Total | 88 | 77 | 16 |
- 1 The technique is useful in identifying low risk patients, only 16 of 88 had further histological investigation.
- 2 Increased experience and better recognition of the different cytological appearances should improve the diagnostic accuracy.
5.
Annelies J. Veraart Anna M. Romaní Elisabet Tornés Sergi Sabater 《Journal of phycology》2008,44(3):564-572
Nutrient input in streams alters the density and species composition of attached algal communities in open systems. However, in forested streams, the light reaching the streambed (rather than the local nutrient levels) may limit the growth of these communities. A nutrient‐enrichment experiment in a forested oligotrophic stream was performed to test the hypothesis that nutrient addition has only minor effects on the community composition of attached algae and cyanobacteria under light limitation. Moderate nutrient addition consisted of increasing basal phosphorus (P) concentrations 3‐fold and basal nitrogen (N) concentrations 2‐fold. Two upstream control reaches were compared to a downstream reach before and after nutrient addition. Nutrients were added continuously to the downstream reach for 1 year. Algal biofilms growing on ceramic tiles were sampled and identified for more than a year before nutrient addition to 12 months after. Diatoms were the most abundant taxonomic group in the three stream reaches. Nutrient enrichment caused significant variations in the composition of the diatom community. While some taxa showed significant decreases (e.g., Achnanthes minutissima, Gomphonema angustum), increases for other taxa (such as Rhoicosphenia abbreviata and Amphora ovalis) were detected in the enriched reach (for taxonomic authors, see Table 2 ). Epiphytic and adnate taxa of large size were enhanced, particularly during periods of favorable growth conditions (spring). Nutrients also caused a change in the algal chl a, which increased from 0.5–5.8 to 2.1–10.7 μg chl · cm?2. Our results indicate that in oligotrophic forested streams, long‐term nutrient addition has significant effects on the algal biomass and community composition, which are detectable despite the low light availability caused by the tree canopy. Low light availability moderates but does not detain the long‐term tendency toward a nutrient‐tolerant community. Furthermore, the effects of nutrient addition on the algal community occur in spite of seasonal variations in light, water flow, and water chemical characteristics, which may confound the observations. Table 2. Percent abundances of the most frequent taxa in three reaches of the Fuirosos stream. U1 and U2 untreated; E, enriched both in the periods before (bef) and after (aft) the enrichment of the E reach. Acronyms identifying the taxa are indicated.
U1‐bef | U1‐aft | U2‐bef | U2‐aft | E‐bef | E‐aft | ||
---|---|---|---|---|---|---|---|
Achnanthes biasolettiana Grunow | ABIA | 1.1 | 1.2 | 0.4 | 0.1 | 5.4 | 0.7 |
Achnanthes lanceolata (Bréb.) Grunow | ALAN | 7.2 | 1.3 | 5.7 | 7.1 | 7.3 | 2.2 |
Achnanthes minutissima Kütz. | AMIN | 56.2 | 55.0 | 81.2 | 71.4 | 52.2 | 34.5 |
Achnanthes lanceolata v. frequentissima Lange‐Bert. | ALFR | 0.0 | 0.1 | 0.1 | 0.9 | 1.0 | 0.0 |
Amphora inariensis Krammer | AINA | 1.9 | 2.0 | 0.3 | 0.1 | 1.0 | 1.4 |
Amphora ovalis (Kütz.) Kütz. | AOVA | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 1.3 |
Amphora pediculus (Kütz.) Grunow | APED | 0.9 | 2.2 | 0.1 | 0.6 | 3.3 | 1.3 |
Cocconeis pediculus Ehrenb. | CPED | 0.1 | 0.2 | 0.0 | 0.1 | 0.2 | 1.7 |
Cocconeis placentula Ehrenb. | CPLA | 13.7 | 20.3 | 1.8 | 8.4 | 12.3 | 32.4 |
Cymbella silesiaca Bleisch in Rabenh. | CSLE | 0.0 | 0.2 | 0.0 | 0.1 | 0.0 | 0.1 |
Diploneis oblongella (Nägeli) Cleve‐Euler | DOBL | 0.6 | 0.0 | 0.9 | 0.2 | 0.0 | 0.0 |
Fragilaria capucina var. gracilis (Øestrup) Hustedt | FCGP | 0.3 | 1.0 | 0.1 | 0.0 | 0.1 | 3.5 |
Fragilaria capucina var. capitellata (Grunow) Lange‐Bert. | FCCP | 0.0 | 0.2 | 0.0 | 0.1 | 0.4 | 0.6 |
Fragilaria ulna (Nitzsch) Lange‐Bert. | FULN | 0.2 | 1.1 | 0.1 | 0.1 | 0.0 | 1.4 |
Gomphonema angustatum (Kütz.) Rabenh. | GADI | 1.6 | 0.6 | 1.6 | 1.8 | 1.0 | 0.8 |
Gomphonema angustum C. Agardh | GANT | 0.2 | 0.1 | 0.6 | 1.2 | 1.4 | 0.1 |
Gomphonema minutum (C. Agardh) C. Agardh | GMIN | 0.2 | 0.0 | 0.3 | 0.1 | 0.3 | 0.5 |
Gomphonema pumilum (Grunow) E. Reichardt et Lange‐Bert. | GPUM | 1.7 | 0.0 | 2.0 | 1.4 | 1.1 | 0.0 |
Meridion circulare (Grev.) C. Agardh | MCIR | 0.0 | 0.1 | 1.5 | 1.7 | 0.4 | 0.2 |
Navicula antonii Lange‐Bert. | NANT | 0.8 | 0.1 | 0.1 | 0.2 | 0.8 | 0.2 |
Navicula accomoda Hust. | NARB | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
Navicula capitatoradiata H. Germ. | NCPR | 0.3 | 0.0 | 0.1 | 0.1 | 0.0 | 0.3 |
Navicula cryptocephala Kütz. | NCRY | 0.5 | 0.1 | 0.1 | 0.3 | 0.5 | 0.2 |
Nitzschia linearis (C. Agardh) W. Sm. | NLIN | 0.2 | 0.0 | 0.0 | 0.2 | 0.0 | 0.1 |
Nitzschia palea (Kütz.) W. Sm. | NPAL | 0.0 | 0.0 | 0.3 | 0.2 | 0.5 | 0.2 |
Reimeria sinuata (W. Greg.) Kociolek et Stoermer | RSIN | 3.4 | 2.0 | 0.6 | 1.2 | 4.9 | 2.8 |
Rhoicosphenia abbreviata (C. Agardh) Lange‐Bert. | RABB | 8.1 | 5.0 | 0.2 | 0.4 | 3.6 | 9.9 |
Citing Literature
Volume 44 , Issue 3 June 2008
Pages 564-572 相似文献
6.
7.
Ok‐Hwan Lee Kye‐Yoon Yoon Kui‐Jin Kim SangGuan You Boo‐Yong Lee 《Journal of phycology》2011,47(3):548-556
Recent studies suggest that seaweed extracts are a significant source of bioactive compounds comparable to the dietary phytochemicals such as onion and tea extracts. The exploration of natural antioxidants that attenuate oxidative damage is important for developing strategies to treat obesity‐related pathologies. The objective of this study was to screen the effects of seaweed extracts of 49 species on adipocyte differentiation and reactive oxygen species (ROS) production during the adipogenesis in 3T3‐L1 adipocytes, and to investigate their total phenol contents and 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) radical scavenging activities. Our results show that high total phenol contents were observed in the extracts of Ecklonia cava (see Table 1 for taxonomic authors) (681.1 ± 16.0 μg gallic acid equivalents [GAE] · g?1), Dictyopteris undulata (641.3 ± 70.7 μg GAE · g?1), and Laurencia intermedia (560.9 ± 48.1 μg GAE · g?1). In addition, DPPH radical scavenging activities were markedly higher in Sargassum macrocarpum (60.2%), Polysiphonia morrowii (55.0%), and Ishige okamurae (52.9%) than those of other seaweed extracts (P < 0.05). Moreover, treatment with several seaweed extracts including D. undulata, Sargassum micracanthum, Chondrus ocellatus, Gelidium amansii, Gracilaria verrucosa, and Grateloupia lanceolata significantly inhibited adipocyte differentiation and ROS production during differentiation of 3T3‐L1 preadipocytes. Furthermore, the production of ROS was positively correlated with lipid accumulation (R2 = 0.8149). According to these preliminary results, some of the seaweed extracts can inhibit ROS generation, which may protect against oxidative stress that is linked to obesity. Further studies are required to determine the molecular mechanism between the verified seaweeds and ROS, and the resulting effects on obesity. Table 1. List of Korean seaweed extracts of 49 species evaluated in this experiment.
Type | No. | Scientific name | Collection time | TP1 (μg GAE · g?1) |
---|---|---|---|---|
Brown macroalgae | SE‐1 | Chondracanthus tenellus (Harv.) Hommers. | April 27, 2006 | 112.8 ± 15.1lm |
SE‐2 | Colpomenia sinusa (F. C. Mertens ex Roth) Derbes et Solier in Castagne | May 11, 2006 | 44.0 ± 4.1opqrs | |
SE‐3 | Dictyopteris divaricata (Okamura) Okamura | April 6, 2006 | 41.5 ± 5.6pqrs | |
SE‐4 | Dictyopteris pacifica (Yendo) I. K. Hwang, H.‐S. Kim et W. J. Lee | April 27, 2006 | 80.9 ± 8.3mno | |
SE‐5 | Dictyopteris prolifera (Okamura) Okamura | November 26, 2007 | 48.4 ± 3.0nopqrs | |
SE‐6 | Dictyopteris undulata Holmes | July 28, 2007 | 641.3 ± 70.7b | |
SE‐7 | Dictyota asiatica I. K. Hwang | April 6, 2006 | 52.9 ± 7.6nonopqr | |
SE‐8 | Ecklonia cava Kjellm. | October 22, 2006 | 681.1 ± 16.0a | |
SE‐9 | Ecklonia stolonifera Okamura | November 26, 2007 | 36.5 ± 3.4pqrs | |
SE‐10 | Endarachne binghamiae J. Agardh | March 10, 2006 | 50.4 ± 2.6nopqrs | |
SE‐11 | Hizikia fusiformis (Harv.) Okamura | July 23, 2006 | 16.4 ± 1.2rs | |
SE‐12 | Hydroclathrus clathratus (C. Agardh) M. Howe | May 11, 2006 | 18.1 ± 0.9rs | |
SE‐13 | Ishige okamurae Yendo | May 26, 2006 | 237.4 ± 1.6h | |
SE‐14 | Lethesia difformis (L.) Aresch. | May 11, 2006 | 11.2 ± 1.9s | |
SE‐15 | Myelophycus simplex (Harv.) Papenf. | April 27, 2006 | 39.5 ± 3.2pqrs | |
SE‐16 | Padina arborescens Holmes | July 29, 2007 | 172.9 ± 23.1ij | |
SE‐17 | Sargassum fulvellum (Turner) C. Agardh | April 27, 2006 | 119.1 ± 5.6kl | |
SE‐18 | Sargassum micracanthum (Kütz.) Endl. | December 21, 2006 | 468.0 ± 22.7e | |
SE‐19 | Sargassum patens C. Agardh | January 21, 2007 | 41.5 ± 5.7pqrs | |
SE‐20 | Sargassum confusum C. Agardh f. validum Yendo | March 8, 2008 | 110.9 ± 3.5lm | |
SE‐21 | Sargassum horneri (Turner) C. Agardh | March 1, 2006 | 84.8 ± 9.4lmn | |
SE‐22 | Sargassum macrocarpum C. Agardh | January 21, 2007 | 353.9 ± 59.1g | |
SE‐23 | Sargassum muticum (Yendo) Fensolt | January 21, 2007 | 72.1 ± 14.9nop | |
SE‐24 | Sargassum nipponium Yendo | April 6, 2006 | 54.0 ± 3.5nopqr | |
SE‐25 | Sargassum sagamianum Yendo | March 8, 2008 | 41.0 ± 6.7pqrs | |
SE‐26 | Sargassum thunbergii (Mertens ex Roth) Kuntze | July 23, 2006 | 27.7 ± 0.8qrs | |
SE‐27 | Scytosiphon gracilis Kogame | May 26, 2006 | 30.2 ± 5.6qrs | |
SE‐28 | Scytosiphon lomentaria (Lyngb.) Link | May 11, 2006 | 66.5 ± 8.9nopq | |
Red macroalgae | SE‐29 | Bonnemaisonia hamifera Har. | April 27, 2006 | 44.1 ± 2.3opqrs |
SE‐30 | Callophyllis crispata Okamura | May 11, 2006 | 37.6 ± 12.6pqrs | |
SE‐31 | Chondria crassicaulis Harv. | May 11, 2006 | 45.4 ± 4.4opqrs | |
SE‐32 | Chondrus crispus Stackh. | May 26, 2006 | 40.7 ± 8.0pqrs | |
SE‐33 | Chondrus ocellatus Holmes | May 11, 2006 | 47.2 ± 1.7nopqrs | |
SE‐34 | Gelidium amansii (J. V. Lamour.) J. V. Lamour. | April 27, 2006 | 525.3 ± 35.9d | |
SE‐35 | Gloioperltis furcata (Postels et Rupr.) J. Agardh | May 26, 2006 | 147.7 ± 6.4jk | |
SE‐36 | Gloioperltis complanta (Harv.) Yamada | May 26, 2006 | 58.2 ± 6.4nopq | |
SE‐37 | Gracilaria verrucosa (Hudson) Papenf. | March 6, 2008 | 55.1 ± 7.5nopqr | |
SE‐38 | Grateloupia elliptica Holmes | May 26, 2006 | 154.4 ± 12.9j | |
SE‐39 | Grateloupia filicina (J. V. Lamour.) C. Agardh | May 11, 2006 | 38.2 ± 2.2pqrs | |
SE‐40 | Grateloupia lanceolata (Okamura) Kawag. | July 23, 2006 | 32.7 ± 3.0pqrs | |
SE‐41 | Laurencia intermedia J. V. Lamour. | May 11, 2006 | 560.9 ± 48.1c | |
SE‐42 | Laurencia intricata J. V. Lamour. | April 27, 2006 | 35.4 ± 4.0pqrs | |
SE‐43 | Laurencia okamurae Yamada | May 11, 2006 | 193.2 ± 41.9i | |
SE‐44 | Lomentaria hakodatensis Yendo | April 27, 2006 | 165.2 ± 15.1ij | |
SE‐45 | Polyopes affinis (Harv.) Kawag. et H.‐W. Wang | May 26, 2006 | 42.9 ± 2.3opqrs | |
SE‐46 | Polysiphonia morrowii Harv. | May 11, 2006 | 392.4 ± 40.3f | |
SE‐47 | Prionitis cornea (Okamura) E. Y. Dawson | October 22, 2006 | 47.9 ± 3.6nopqrs | |
Green macroalgae | SE‐48 | Enteromorpha prolifera (O. F. Müll.) J. Agardh | March 26, 2006 | 42.0 ± 5.3pqrs |
SE‐49 | Ulva pertusa Kjellm. | April 27, 2006 | 48.3 ± 3.8nopqrs |
- GAE, gallic acid equivalents; SE, seaweed extracts.
- 1TP, total phenol content is micrograms of total phenol contents per gram of seaweed extract based on gallic acid as standard. The values are means ± SD from three replications.
- a–sMeans in the same column not sharing a common letter are significantly different (P < 0.05) by Duncan’s multiple test.
Citing Literature
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- Ana Rocío Múzquiz de la Garza, Mireya Tapia-Salazar, Maribel Maldonado-Muñiz, Julián de la Rosa-Millán, Janet Alejandra Gutiérrez-Uribe, Liliana Santos-Zea, Bertha Alicia Barba-Dávila, Denis Ricque-Marie, Lucía Elizabeth Cruz-Suárez, Nutraceutical Potential of Five Mexican Brown Seaweeds, BioMed Research International, 10.1155/2019/3795160, 2019 , (1-15), (2019). Crossref
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- Fook Yee Chye, Birdie Scott Padam, Seah Young Ng, Innovation and Sustainable Utilization of Seaweeds as Health Foods, Sustainability Challenges in the Agrofood Sector, 10.1002/9781119072737, (390-434), (2017). Wiley Online Library
- Gaurav Rajauria, Lynn Cornish, Francesco Ometto, Flower E. Msuya, Raffaella Villa, Identification and selection of algae for food, feed, and fuel applications, Seaweed Sustainability, 10.1016/B978-0-12-418697-2.00012-X, (315-345), (2015). Crossref
- Jatinder Sangha, Owen Wally, Arjun Banskota, Roumiana Stefanova, Jeff Hafting, Alan Critchley, Balakrishnan Prithiviraj, A Cultivated Form of a Red Seaweed (Chondrus crispus), Suppresses β-Amyloid-Induced Paralysis in Caenorhabditis elegans, Marine Drugs, 10.3390/md13106407, 13 , 10, (6407-6424), (2015). Crossref
- Jung-Ae Kim, Fatih Karadeniz, Byul-Nim Ahn, Myeong Sook Kwon, Ok-Ju Mun, Mihyang Kim, Sang-Hyeon Lee, Ki Hwan Yu, Yuck Yong Kim, Chang-Suk Kong, Sargassum sp. Attenuates Oxidative Stress and Suppresses Lipid Accumulation in vitro, Journal of Life Science, 10.5352/JLS.2014.24.3.274, 24 , 3, (274-283), (2014). Crossref
- Georgia M. Hart, Tamara Ticktin, Dovi Kelman, Anthony D. Wright, Nicole Tabandera, Contemporary Gathering Practice and Antioxidant Benefit of Wild Seaweeds in Hawai’i, Economic Botany, 10.1007/s12231-014-9258-7, 68 , 1, (30-43), (2014). Crossref
- Zahid Manzoor, Vivek Bhakta Mathema, Doobyeong Chae, Eun-Sook Yoo, Hee-Kyoung Kang, Jin-Won Hyun, Nam Ho Lee, Mi-Hee Ko, Young-Sang Koh, Extracts of the seaweed Sargassum macrocarpum inhibit the CpG-induced inflammatory response by attenuating the NF-κB pathway, Food Science and Biotechnology, 10.1007/s10068-014-0041-4, 23 , 1, (293-297), (2013). Crossref
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- Min‐Jung Seo, Hyeon‐Son Choi, Ok‐Hwan Lee, Boo‐Yong Lee, Grateloupia lanceolata (Okamura) Kawaguchi, the Edible Red Seaweed, Inhibits Lipid Accumulation and Reactive Oxygen Species Production During Differentiation in 3T3‐L1 Cells, Phytotherapy Research, 10.1002/ptr.4765, 27 , 5, (655-663), (2012). Wiley Online Library
- Mi‐Seon Woo, Hyeon‐Son Choi, Ok‐Hwan Lee, Boo‐Yong Lee, The Edible red Alga, Gracilaria verrucosa, Inhibits Lipid Accumulation and ROS Production, but Improves Glucose Uptake in 3T3‐L1 Cells, Phytotherapy Research, 10.1002/ptr.4813, 27 , 7, (1102-1105), (2012). Wiley Online Library
- Young-Jun Lee, Bo-Ra Yoon, Hyeon-Son Choi, Boo-Yong Lee, Ok-Hwan Lee, Effect of Sargassum micracanthum extract on Lipid Accumulation and Reactive Oxygen Species (ROS) Production during Differentiation of 3T3-L1 Preadipocytes, Korean Journal of Food Preservation, 10.11002/kjfp.2012.19.3.455, 19 , 3, (455-461), (2012). Crossref
- Mei Piao, Yu Hyun, Suk Cho, Hee Kang, Eun Yoo, Young Koh, Nam Lee, Mi Ko, Jin Hyun, An Ethanol Extract Derived from Bonnemaisonia hamifera Scavenges Ultraviolet B (UVB) Radiation-Induced Reactive Oxygen Species and Attenuates UVB-Induced Cell Damage in Human Keratinocytes, Marine Drugs, 10.3390/md10122826, 10 , 12, (2826-2845), (2012). Crossref
Volume 47 , Issue 3 June 2011
Pages 548-556 相似文献
8.
Differential scanning calorimetry was used to directly determine the transition enthalpies accompanying the duplex-to-single-strand transition of poly[d(AT)], poly(dA)·poly(dT), poly[d(AC)]·poly[d(TG)], and d(GCGCGC). The calorimetric data allow us to define the following average base-stacking enthalpies:
Comparison with published data on the corresponding RNA interactions reveals remarkably good agreement. By assuming transition enthalpies to result from the pairwise disruption of nearest-neighbor stacking interactions, we used the enthalpy data listed above to predict the transition enthalpies for three oligomeric DNA duplexes. Excellent agreement was found between the predicted and the calorimetrically determined values. 相似文献
Interaction | ΔH (kcal/stack) |
---|---|
AC/TG, TG/AC | 5.6 |
AT/TA, TA/AT | 7.1 |
AA/TT | 8.6 |
GC/CG, CG/GC | 11.9 |
9.
Root System Markup Language: Toward a Unified Root Architecture Description Language 总被引:1,自引:0,他引:1
Guillaume Lobet Michael P. Pound Julien Diener Christophe Pradal Xavier Draye Christophe Godin Mathieu Javaux Daniel Leitner Félicien Meunier Philippe Nacry Tony P. Pridmore Andrea Schnepf 《Plant physiology》2015,167(3):617-627
10.
Microevolutionary interpretations from the dentition 总被引:2,自引:0,他引:2
C G Turner 《American journal of physical anthropology》1969,30(3):421-426
The qualities of the dentition (preservability, hereditability, evolutionary stability, behavioral correlations) make them eminently suited for long-term evolutionary studies where only natural selection need be considered. Teeth, however, can be just as valuable for short-term or microevolutionary studies but here consideration of additional processes and conditions is required. Admixture, one such source of microevolution common to many living human groups, often follows contact or discovery by external groups or displacement. Estimates of admixture have been made for several such living hybrid populations by several investigators and all were premised on assumed blood group allele frequencies for the ancestral population of the hybrid group as in this simple model:
For any given living hybrid group, admixture estimates vary widely depending on which other living “unmixed” population is assumed to be most like the ancestral group. This paper presents admixture estimates (for three living North American native groups, Aleuts, Koniag Eskimos, and Pueblo Indians, known by pedigree studies to be admixed to varying degrees) based on allele frequencies calculated from dental phenotypes exhibited in prehistoric skeletal samples known to be ancestral to each of the living hybrid groups. These estimates are compared with estimates based on blood groups. It is uncertain at present whether the potential error resulting from possibly inexact modes of inheritance of these dental traits is less or greater than the potential error due to assuming a living group to be genetically identical with the ancestral group for the trait(s) in question. Widespread acceptance of this procedure utilizing skeletal samples obviously rests with the verification of existing models for dental trait inheritance. 相似文献
Time 3 | living hybrid groups |
Time 2 | + foreign group |
Time 1 | prehistoric ancestral group |
11.
Hundreds of eukaryotic membrane proteins are anchored to membranes by a single transmembrane domain at their carboxyl terminus. Many of these tail-anchored (TA) proteins are posttranslationally targeted to the endoplasmic reticulum (ER) membrane for insertion by the guided-entry of TA protein insertion (GET) pathway. In recent years, most of the components of this conserved pathway have been biochemically and structurally characterized. Get3 is the pathway-targeting factor that uses nucleotide-linked conformational changes to mediate the delivery of TA proteins between the GET pretargeting machinery in the cytosol and the transmembrane pathway components in the ER. Here we focus on the mechanism of the yeast GET pathway and make a speculative analogy between its membrane insertion step and the ATPase-driven cycle of ABC transporters.The mechanism of membrane protein insertion into the endoplasmic reticulum (ER) has been extensively studied for many years (Shao and Hegde 2011). From this work, the signal recognition particle (SRP)/Sec61 pathway has emerged as a textbook example of a cotranslational membrane insertion mechanism (Grudnik et al. 2009). The SRP binds a hydrophobic segment (either a cleavable amino-terminal signal sequence or a transmembrane domain) immediately after it emerges from the ribosomal exit tunnel. This results in a translational pause that persists until SRP engages its receptor in the ER and delivers the ribosome-nascent chain complex to the Sec61 channel. Last, the Sec61 channel enables protein translocation into the ER lumen along with partitioning of hydrophobic transmembrane domains into the lipid bilayer through the Sec61 lateral gate (Rapoport 2007).Approximately 5% of all eukaryotic membrane proteins have an ER targeting signal in a single carboxy-terminal transmembrane domain that emerges from the ribosome exit tunnel following completion of protein synthesis and is not recognized by the SRP (Stefanovic and Hegde 2007). Nonetheless, because hydrophobic peptides in the cytoplasm are prone to aggregation and subject to degradation by quality control systems (Hessa et al. 2011), these tail-anchored (TA) proteins still have to be specifically recognized, shielded from the aqueous environment, and guided to the ER membrane for insertion. In the past five years, the guided-entry of TA proteins (GET) pathway has come to prominence as the major machinery for performing these tasks and the enabler of many key cellular processes mediated by TA proteins including vesicle fusion, membrane protein insertion, and apoptosis. This research has rapidly yielded biochemical and structural insights (and2)2) into many of the GET pathway components (Hegde and Keenan 2011; Chartron et al. 2012a; Denic 2012). In particular, Get3 is an ATPase that uses metabolic energy to bridge recognition of TA proteins by upstream pathway components with TA protein recruitment to the ER for membrane insertion. However, the precise mechanisms of nucleotide-dependent TA protein binding to Get3 and how the GET pathway inserts tail anchors into the membrane are still poorly understood. Here, we provide an overview of the budding yeast GET pathway with emphasis on mechanistic insights that have come from structural studies of its membrane-associated steps and make a speculative juxtaposition with the ABC transporter mechanism.
Open in a separate windowTA, tail anchored; TPR, tetratricopeptide repeat; TMDs, transmembrane domains.
Open in a separate windowADP, adenosine diphosphate. 相似文献
Table 1.
A catalog of GET pathway component structuresComponent | Role in the pathway | PDB ID |
---|---|---|
Sgt2 | Component of the pretargeting complex that delivers TA proteins to Get3; dimer interacts with Get4/Get5, contains TPR repeats that interact with Hsps | 3SZ7 |
Get5 | Component of the pretargeting complex that delivers TA proteins to Get3; dimer interacts with Get4 via amino-terminal domain and with Sgt2 via its ubiquitin-like domain | 2LNZ 3VEJ 2LO0 |
Get4 | Component of the pretargeting complex that delivers TA proteins to Get3; interacts with Get3 via amino-terminal domain and with Get4 via carboxy-terminal domain | 3LPZ 3LKU 3WPV |
Get3 | ATPase that binds the TA protein; dimer interacts with the pretargeting complex in the cytosol, and with Get1/2 at the ER membrane | Table 2 |
Get1 | ER receptor for Get3; integral ER membrane protein, three TMDs; forms a complex with Get2 | 3SJA, 3SJB 3SJC, 3ZS8 3VLC, 3B2E |
Get2 | ER receptor for Get3; integral ER membrane protein, three TMDs; forms a complex with Get1 | 3SJD 3ZS9 |
Table 2.
An itemized list of published Get3 structures with associated nucleotides and conformation nomenclatureOrganism | Nucleotide | Conformation | PDB ID | References |
---|---|---|---|---|
Get3 | ||||
Schizosaccharomyces pombe | None | Open | 2WOO | Mateja et al. 2009 |
Saccharomyces cerevisiae | None | Open | 3H84 | Hu et al. 2009 |
3A36 | Yamagata et al. 2010 | |||
Aspergillus fumigatus | ADP | Open | 3IBG | Suloway et al. 2009 |
S. cerevisiae | ADP | Open | 3A37 | Yamagata et al. 2010 |
Debaryomyces hansenii | ADP | Closed | 3IO3 | Hu et al. 2009 |
Chaetomium thermophilum | AMPPNP-Mg2+ | Closed | 3IQW | Bozkurt et al. 2009 |
C. thermophilum | ADP-Mg2+ | Closed | 3IQX | Bozkurt et al. 2009 |
S. cerevisiae | ADP•AlF4−-Mg2+ | Fully closed | 2WOJ | Mateja et al. 2009 |
Methanothermobacter thermautotrophicus | ADP•AlF4−-Mg2+ | Fully closed | 3ZQ6 | Sherill et al. 2011 |
Methanococcus jannaschii | ADP•AlF4−-Mg2+ | Tetrameric | 3UG6 | Suloway et al. 2012 |
3UG7 | ||||
Get3/Get2cyto | ||||
S. cerevisiae | ADP-Mg2+ | Closed | 3SJD | Stefer et al. 2011 |
S. cerevisiae | ADP•AlF4−-Mg2+ | Closed | 3ZS9 | Mariappan et al. 2011 |
Get3/Get1cyto | ||||
S. cerevisiae | None | Semiopen | 3SJC | Stefer et al. 2011 |
S. cerevisiae | ADP | Semiopen | 3VLC | Kubota et al. 2012 |
S. cerevisiae | None | Open | 3SJA | Stefer et al. 2011 |
3SJB | Stefer et al. 2011 | |||
3ZS8 | Mariappan et al. 2011 | |||
ADP | Open | 3B2E | Kubota et al. 2012 |
12.
Fengzhen Yang Qi Zhao Lipeng Wang Jinying Wu Lihua Jiang Li Sheng Leyan Zhang Zhaoping Xue Maoli Yi 《Polish journal of microbiology》2022,71(2):251
Cefoperazone/sulbactam (CSL) and piperacillin/tazobactam (TZP) are commonly used in clinical practice in China because of their excellent antimicrobial activity. CSL and TZP-nonsusceptible Enterobacteriaceae are typically resistant to extended-spectrum cephalosporins such as ceftriaxone (CRO). However, 11 nonrepetitive Enterobacteriaceae strains, which were resistant to CSL and TZP yet susceptible to CRO, were collected from January to December 2020. Antibiotic susceptibility tests and whole-genome sequencing were conducted to elucidate the mechanism for this rare phenotype. Antibiotic susceptibility tests showed that all isolates were amoxicillin/clavulanic-acid resistant and sensitive to ceftazidime, cefepime, cefepime/tazobactam, cefepime/zidebactam, ceftazidime/avibactam, and ceftolozane/tazobactam. Whole-genome sequencing revealed three of seven Klebsiella pneumoniae strains harbored blaSHV-1 only, and four harbored blaSHV-1 and blaTEM-1B. Two Escherichia coli strains carried blaTEM-1B only, while two Klebsiella oxytoca isolates harbored blaOXY-1-3 and blaOXY-1-1, respectively. No mutation in the β-lactamase gene and promoter sequence was found. Outer membrane protein (Omp) gene detection revealed that numerous missense mutations of OmpK36 and OmpK37 were found in all strains of K. pneumoniae. Numerous missense mutations of OmpK36 and OmpK35 and OmpK37 deficiency were found in one K. oxytoca strain, and no OmpK gene was found in the other. No Omp mutations were found in E. coli isolates. These results indicated that narrow spectrum β-lactamases, TEM-1, SHV-1, and OXY-1, alone or in combination with Omp mutation, contributed to the resistance to CSL and TZP in CRO-susceptible Enterobacteriaceae.Antibiotic susceptibility tests
Open in a separate windowCROceftriaxone, CAZceftazidime, FEPcefepime, AMC:amoxicillin clavulanic-acid, CSLcefoperazone/sulbactam, TZP:piperadllin/tazobactam, FPT:cefepime tazobactam, FPZ:cefepime/zidebactam, CZA:ceftazidime/avibactam, CZTceftolozane/tazobactam Gene sequencing results
Open in a separate window 相似文献
Antibiotics | Breakpoint, (μg/ml) | Klebsiella pneumoniae | Escherichia cou | Klebriehd axyoca | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
E1 | E3 | E4 | E7 | E9 | E10 | E11 | E6 | E8 | E2 | E5 | ||
CRO | ≤1≥4 | ≤0.5 | ≤0.5 | ≤0.5 | ≤0.5 1 | ≤0.5 | 1 | ≤0.5 | ≤0.5 | 1 | 1 | |
CAZ | 4 ≥16 | 1 | 2 | 1 | 4 | 4 | 4 | 4 | 2 | 4 | 1 1 | |
FEP | ≤2 216 1 | 1 | 0.25 | 1 | 2 | 2 | 2 | 0.5 | 2 | 1 1 | ||
AMC | ≤8 ≥32 | ≥128 | ≥128 | ≥128 | ≥128 | ≥128 | ≥128 | ≥128 | ≥128 | ≥128 | ≥128 | ≥128 |
CSL | ≤16 ≥64 | 64 | 64 | 64 | 64 | ≥128 | 128 | ≥128 | 64 | 128 | 128 | ≥128 |
TZP | ≤16 ≥128 | ≥256 | ≥256 | ≥256 | ≥256 | 2256 | 2256 | ≥256 | ≥256 | ≥256 | ≥256 | ≥256 |
FPT | ≤2 ≥16 | 1 | 0.5 | 0.06 | 0.125 | 2 | 1 | 2 | 0.25 | 1 | 0.125 | 0.25 |
FPZ | ≤2 216 | 0.25 | 0.25 | 0.06 | 0.125 | 0.25 | 0.25 1 | 0.125 | 0.25 | 0.125 | 0.125 | |
CZA | ≤8 216 1 | 0.5 | 0.25 | 0.25 | 1 | 0.25 | 1 | 0.5 | 0.5 | 0.5 | 0.25 | |
CZT | ≤2 28 | 2 | 1 | 0.5 1 | 2 | 2 | 2 1 | 1 | 2 | 2 |
Number | Strain | ST | p-Lactamase gene | Promoter sequence mutation | Omp mutation |
---|---|---|---|---|---|
El | Kpn | 45 | blaSHV-1, blaTEM-lB | none | OmpK36, OmpK3 7 |
E3 | Kpn | 45 | blaSHV-1, blaTEM-lB | none | OmpK36. OmpK3 7 |
E4 | Kpn | 2854 | blaSHV-1 | none | OmpK36, OmpK3 7 |
E7 | Kpn | 2358 | blaSHV-1 - blaTEM-lB | none | OmpK36, OmpK3 7 |
E9 | Kpn | 2358 | blaSHV-1. blaTEM-lB | none | OmpK36. OmpK3 7 |
E10 | Kpn 18 | 9 | blaSHV-1 | none | OmpK36. OmpK3 7 |
Ell | Kpn | 45 | blaSHV-1 | none | OmpK36, OmpK3 7 |
E6 | Eco | 88 | blaTEM-lB | none | none |
ES | Eco | 409 | blaTEM-1B | none | none |
E2 | Kox | 194 | blaOXY-1-3 | none | OmpK36 mutations. OmpK35 and OmpK 37 deficiency |
E5 | Kox 11 | blaOXY-1-1 | none | no OmpK (OmpK3 5, OmpK36 and OmpK37) gene found |
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16.
Christopher N. Carender Christopher A. Anthony Edward O. Rojas Nicolas O. Noiseux Nicholas A. Bedard Timothy S. Brown 《The Iowa orthopaedic journal》2022,42(1):169
BackgroundPreoperative counseling may reduce postoperative opioid requirements; however, there is a paucity of randomized controlled trials (RCTs) demonstrating efficacy. The purpose of this study was to perform an interventional, telehealth-based RCT evaluating the effect of peri-operative counseling on quantity and duration of opioid consumption following primary total joint arthroplasty (TJA).MethodsParticipants were randomized into three groups: 1. Control group, no perioperative counseling; 2. Intervention group, preoperative educational video; 3. Intervention group, preoperative educational video and postoperative acceptance and commitment therapy (ACT). Opioid consumption was evaluated daily for 14 days and at 6 weeks postoperatively. Best-case and worse-case intention to treat analyses were performed to account for non-responses. Bonferroni corrections were applied.Results183 participants were analyzed (63 in Group 1, 55 in Group 2, and 65 in Group 3). At 2 weeks postoperatively, there was no difference in opioid consumption between Groups 1, 2, and 3 (p>0.05 for all). At 6 weeks postoperatively, Groups 2 and 3 had consumed significantly less opioids than Group 1 (p=0.04, p<0.001) (Variable Group p-value 1. Control 2. Video Only Video + ACT Sex (n, % female) 39 (62%) 32 (58%) 40 (62%) 0.90 Surgery (n, % THA) 26 (41%) 21 (38%) 31 (47%) 0.56 Age (mean ± SD; years) 59 ± 11 59 ± 11 58 ± 9 Overall: 0.83
1v2: 0.98
2v3: 0.65
2v3: 0.56 Prolonged Opioid Use > 60 mo. (n, %) 0 0 0 - Opioid Use Within 3 mo. of Index Surgery (n, %) 0 (14%) 4 (7%) 5 (8%) 0.34
1v2: 0.98
2v3: 0.65
2v3: 0.56