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短梗霉胞外多糖发酵及其发酵动力学 总被引:6,自引:0,他引:6
对短梗霉(Aureobasidiumpullulans)胞外多糖(EPS)的发酵及其发酵动力学进行了研究。短梗霉菌体前期生长速度较快,到48b生长趋于稳定期,其胞外多糖合成随菌体生长的不断上升,到84b多糖的产量达到最高,为14.24(g/L)发酵实验基于Logitic方程和Luedeking-Piret方程,得到了描述发酵过程的动力学数学模型和模型参数,同时对实验数据与模型进行了验证比较。 相似文献
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银杏是我国的特有物种,银杏外种皮一直以来都是被作为废弃物被丢弃,本实验通过采用微波辅助的方法提取了外种皮多糖,改进了传统的浸提方法。通过研究微波时间、料液比和提取时间对银杏多糖提取的影响,在单因素轮换法基础上采取响应面法优化实验。结果表明,料液比29,在功率700 W下微波处理13 min,然后在沸水浴中浸提2.75 h,银杏外种皮的收率为13.913%。 相似文献
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以出芽短梗霉IFO 4464为实验菌种,采用响应面法(RSM)优化了出芽短梗霉IFO 4464产普鲁兰多糖的发酵培养基。通过实验得到出芽短梗霉最佳发酵培养基为蔗糖59.8g/L,硫酸铵0.7 g/L,硫酸镁0.3 g/L,磷酸二氢钾5.0g/L,氯化钾0.5g/L,氯化钠1.5g/L,酵母浸膏2.5 g/L,多糖产量可达21.92 g/L。 相似文献
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通过单因素和响应面法优化淡水微藻Chlorella vulgaris 224胞外多糖的积累,并对其抑菌、抗氧化活性进行研究。经优化后,C.vulgaris 224积累胞外多糖的最佳条件为NaNO3 203.13 mg/L、NaCl 85.34 mg/L、NaHCO3 2.13 g/L;在此条件下,其胞外多糖产量为70.365 7 mg/L,比优化前提高了1.62倍。抑菌实验结果表明,C.vulgaris 224的胞外多糖对金黄色葡萄球菌有较强的抑菌作用,对白色葡萄球菌、绿脓杆菌和大肠杆菌有较弱的抑菌作用。抗氧化实验结果表明C.vulgaris 224的胞外多糖具有较强的抗氧化活性。本研究为筛选天然抗菌、抗氧化活性物质提供了一定的参考价值,同时为产油微藻的综合开发利用提供了一定的理论依据。 相似文献
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为优化发酵虫草菌粉多糖的微波辅助提取工艺,在单因素实验基础上,以液固比、微波功率以及提取时间为自变量,多糖提取率为响应值,采用中心组合设计的方法,研究各自变量及其交互作用对多糖提取率的影响。利用SAS软件和响应面分析相结合的方法对发酵虫草菌粉多糖的微波辅助提取工艺进行优化,确定了微波辅助提取多糖的最佳条件:液固比值12.2,微波功率650.5W,提取时间11.8min,在此条件下,多糖提取率达到6.41%。采用此法提取的虫草菌丝体多糖,当质量浓度为1mg/mL时,对二苯代苦味肼基自由基(DPPH)清除率达到76%。 相似文献
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经单因素和正交试验优化,灵芝胞外多糖最佳发酵培养基各成分质量分数为:麦芽糖2%,黄豆粉1%,FeSO4·7H2O0.02%,KH2PO40.1%,土豆汁体积分数30%,pH自然,产量可达到86.36g·L-1(湿重)。灵芝胞外多糖产量受发酵过程各因素的影响,发酵过程中pH、总糖、还原糖和氨基氮有一定的相关性。灵芝多糖整个发酵过程需要144h左右,第6d达到发酵终点。 相似文献
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假单胞菌胞外多糖发酵条件的研究 总被引:6,自引:0,他引:6
研究了假单胞菌(Pseudomonassp.6)利用木糖产胞外多糖的发酵条件。实验表明KH2PO4,O2和高碳氮比对多糖合成有促进作用,在发酵后期补加木糖有助于多糖产量的提高 相似文献
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响应面法优化福鸽霉素发酵培养基 总被引:1,自引:0,他引:1
采用Plackett-Burman设计法,对影响纤维堆囊菌So ceMWXAB-125产生福鸽霉素的9个因素进行了筛选。结果表明,影响该菌产生福鸽霉素的主要营养因素为马铃薯淀粉、CaCl2和脱脂奶粉。在此基础上,采用响应面法对其中3个显著因子的最佳水平范围进行研究,利用Design-Expert软件进行二次回归分析得知,马铃薯淀粉、CaCl2和脱脂奶粉的质量浓度分别为8.05、2.72和10.00 g/L时,福鸽霉素的产量从67 mg/L提高到119.98 mg/L。 相似文献
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Aims: To evaluate the influence of environmental parameters on the production of antibiotics (xenocoumacins and nematophin) by Xenorhabdus nematophila and enhance the antibiotic activity. Methods and Results: Response surface methodology (RSM) was employed to study the effects of five parameters (the initial pH, medium volume in flask, rotary speed, temperature and inoculation volume) on the production of antibiotics in flask cultures by X. nematophila YL001. A 25?1‐factorial central composite design was chosen to explain the combined effects of the five parameters and to design a minimum number of experiments. The experimental results and software‐predicted values of production of antibiotics were comparable. The statistical analysis of the results showed that, in the range studied, medium volume in flask, rotary speed, temperature and inoculation volume had a significant effect (P < 0·05) on the production of antibiotics at their individual level, medium volume in flask and rotary speed showed a significant influence at interactive level and were most significant at individual level. The maximum antibiotic activity was achieved at the initial pH 7·64, medium volume in 250 ml flask 25 ml, rotary speed of 220 rev min?1, temperature 27·8°C and inoculation volume of 15·0%. Maximum antibiotic activity of 331·7 U ml?1 was achieved under the optimized condition. Conclusions: As far as known, there are no reports of production of antibiotic from X. nematophila by engineering the condition of fermentation using RSM. The results strongly support the use of RSM for fermentation condition optimization. The optimization of the environmental parameters resulted not only in a 43·4% higher antibiotic activity than unoptimized conditions but also in a reduced amount of the experiments. The chosen method of optimization of fermentation condition was efficient, relatively simple and time and material saving. Significance and Impact of the Study: This study should contribute towards improving the antibiotics activity of X. nematophila. Integrated into a broader study of the impact of environmental factors on the production of antibiotic, this work should help to build more rational control strategy, possibly involving scale‐up of production of antibiotics by X. nematophila. 相似文献
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采用响应面分析方法,对阿萨希丝孢酵母(Trichosporon asahii)ZZB-1产酰胺酶的发酵培养基进行了优化。运用单N子试验筛选出麦芽糖和酵母浸膏为最适碳源、氮源,金属离子Ca^2+、Mn^2+可提高发酵酰胺酶产量;通过最陡爬坡实验逼近以上4个因子的最大响应区域后,采用Box—Behnken响应面分析法,确定产酰胺酶最佳发酵培养基为麦芽糖18.84g/L、酵母浸膏9.55g/L、NaC15g/L、KH2PO41g/L、MgSO4·7H2O0.2g/L、FeS040.001g/L、CaC0370.84μmol/L、MnS0465.39肚mo[/L(1%丙烯酸诱导),NH4·H2O调节pH至7.0。培养基优化后酰胺酶产量由初始2554U/L提高到4156U/L,为原始发酵培养基配方酶活产量的1.63倍。 相似文献
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Venkataraman Deepak Sureshbabu Ram Kumar Pandian Shiva D. Sivasubramaniam Hariharan Nellaiah 《Preparative biochemistry & biotechnology》2016,46(3):288-297
Colorectal cancer (CRC) is one of the leading causes of cancer-related deaths in the Western world. Recently, much attention has been focused on decreasing the risk of CRC by consuming probiotics. In the present study, exopolysaccharide (EPS) extracted from Lactobacillus acidophilus was found to inhibit the growth of CaCo2 colon cancer cell line in a dose-dependent manner. The experiment was performed in both normoxic and hypoxic conditions, and EPS was found to reduce the survival of CaCo2 cell line in both the conditions. Quantitative polymerase chain reaction (qPCR) studies demonstrated that EPS treatment upregulated the expression of peroxisome proliferator activator receptor-γ (PPAR-γ) in both normoxia and hypoxia conditions, whereas it upregulated the expression of erythropoietin (EPO) in the normoxic condition, but there was no significant expression under hypoxic conditions. Hence, the EPS production was optimized by Plackett–Burman design followed by central composite rotatory design. The optimized production of EPS at 24 hr was found to be 400 mg/L. During batch cultivation the production peaked at 21 hr, resulting in an EPS concentration of 597 mg/L. 相似文献
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采用响应面法对产生抑菌活性物质的波赛链霉菌(Streptomyces peucetius)菌株JMC 06001的发酵培养基进行优化。首先采用Minnimum Run Equireplicated Res IV设计对初始发酵培养基的8个营养因素进行筛选,获得影响产生抑菌活性物质的3个主要影响因素:葡萄糖、大豆粉和NaCI;然后用最陡爬坡实验快速逼近最大响应区域;最后,结合Box-Behnken设计及响应面分析,确定主要影响因素的最佳浓度,得出该菌株产抑菌活性物质的最优发酵培养基配方为:葡萄糖1.2%,麦芽糖0.7%,蛋白胨0.9%,大豆粉1.4%,NaCl3.7%,CaCO3 0.1%,复合盐A液2.0%,复合盐B液0.1%,起始pH值7.0。用优化后的培养基发酵所得发酵液对敏感指示菌藤黄八叠球菌的抑菌圈直径达31.5mm,与预测值的相对偏差仅为1.59%,与用初始发酵培养基发酵所得发酵液的抑菌效果(抑菌圈直径26.5mm)相比提高了18.9%。 相似文献
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以黑土豆为原料提取色素,在乙醇浓度、回流温度、回流时间和料液比四个单因素实验基础上,通过响应面法优化提取条件,利用 Box-Benhnken 设计模型,研究四个自变量之间的影响.结果表明,得到最佳提取工艺为回流温度47℃,回流时间47.5 min,料液比1∶90,乙醇浓度42.5%.在此条件下,理论上测得黑土豆中色素的吸光度值为0.413,实验测得黑土豆中色素的吸光度值为0.416,理论值与实验值的相对误差为0.726%.该提取工艺简便、具有较高的重现性和可行性. 相似文献
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为了对荷叶离褶伞产漆酶条件进行优化,在单因素实验基础上,通过最陡爬坡实验(PB)对培养基8因素进行筛选,获得影响产漆酶的3个显著性因素:葡萄糖,pH和KH2PO4;通过中心组合(CCD)设计及响应面分析确定了最优发酵条件:葡萄糖20.09g/L,酪蛋白1.5g/L,酵母提取物1.5g/L,MgSO4 3g/L,CuSO4 3.75mg/L,KH2PO4 3.97g/L,pH 4.98,VB1 0.1g/L,愈创木酚12mg/L,该条件下,漆酶酶活为829.83U/mL,较未优化对照提高46.6%. 相似文献
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对汽爆玉米秸秆同步酶解发酵生产乙醇的条件进行优化。首先利用Fractional Factorial设计法对影响乙醇产量的7个因素进行评价,筛选出具有显著效应的3个因素,即反应温度、酶添加量、总反应时间,再以Box—Behnken设计法及响应面分析法确定主要因素的最佳水平,即反应温度37℃,每g纤维素添加纤维素酶32u,反应时间87h,此时乙醇体积分数达到3.69%。新工艺条件实验结果表明,乙醇体积分数在87h可达到3.76%,和原工艺相比,反应时间缩短了9h,乙醇体积分数提高了13%。 相似文献