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1.
Revin V. V. Kadimaliev D. A. Shutova V. V. Samuilov V. D. 《Applied Biochemistry and Microbiology》2002,38(5):450-453
The treatment of sawdust with the fungus Panus tigrinus VKM F-3616 D changed the contents of functional groups in lignin from wood raw material. These changes are accompanied by the release of carboxyl and phenyl hydroxyl groups involved in chemical bond formation between wood particles in pressed materials manufactured from wood wastes. 相似文献
2.
Kadimaliev DA Revin VV Atykian NA Samuilov VD 《Prikladnaia biokhimiia i mikrobiologiia》2003,39(5):555-560
Lignin consumption and synthesis of lignolytic enzymes by the fungus Panus (Lentinus) tigrinus cultivated on solid phase (modified and unmodified birch and pine sawdusts) were studied. The fungus grew better and consumed more readily the birch lignin than the pine wood. Peroxidase activity was higher in the case of pine sawdust; laccase and lignolytic activities, in the case of birth sawdust. Treatment with ammonia or sulfuric acid decreased lignin consumption by the fungus cultivated on either medium. Modification of sawdust by ultrasound increased lignin consumption and may be recommended for accelerating biodegradation of lignocellulose substrates. 相似文献
3.
Revin VV Kadimaliev DA Shutova VV Samuilov VD 《Prikladnaia biokhimiia i mikrobiologiia》2002,38(5):529-533
The treatment of sawdust with the fungus Panus tigrinus VKM F-3616 D changed the contents of functional groups in lignin from wood raw material. These changes are accompanied by the release of carboxyl and phenyl hydroxyl groups involved in chemical bond formation between wood particles in pressed materials manufactured from wood wastes. 相似文献
4.
V. V. Shutova V. V. Revin Yu. A. Myakushina 《Applied Biochemistry and Microbiology》2008,44(6):619-623
The basidiomycete Lentinus tigrinus was cultured in media containing copper ions added at different growth stages. Copper ions at increased concentrations decelerated of the fungal biomass accumulation. The later Cu2+ ions were added, the better the fungal mycelium developed, and the toxic effect of Cu2+ was less pronounced. The maximum laccase activity (47 U/ml) was observed in the presence of 1.5–2.0 mM Cu2+ added on day 4 of cultivation. 相似文献
5.
D. A. Kadimaliev O. S. Nadezhina N. A. Atykyan V. V. Revin V. D. Samuilov 《Microbiology》2006,75(5):563-567
Lipid composition, intracellular products of lipid peroxidation (LPO), and the activities of extracellular enzymes were studied during submerged cultivation of the xylotrophic fungus Lentinus (Panus) tigrinus VKMF-3616D. The maximum secretion of ligninolytic enzymes during the phase of active mycelium growth correlated with increased content of readily oxidized phospholipids and unsaturated fatty acids and with low content of the LPO products. In the idiophase, which was characterized by lower excretion of extracellular ligninolytic enzymes, the content of more stable phospholipids, saturated fatty acids, and LPO products increased. A relationship between the composition of mycelial lipids and the secretion of ligninolytic enzymes was revealed. 相似文献
6.
Kadimaliev DA Revin VV Atykian NA Nadezhina OS Parshin AA 《Prikladnaia biokhimiia i mikrobiologiia》2011,47(1):73-78
The possibility of the usage of Lentinus tigrinus fungus strain VKM F-3616D for biodegradation of high (up to 5%) phenol concentrations in liquid medium and the involvement of laccase and peroxidase in this process have been studied. L. tigrinus fungus was demonstrated to effectively digrade phenol with easy biomass separation from the liquid. Decrease in phenol concentration was accompanied by increased secretion level and laccase activity at the preliminary stages of biodegradation, while that of peroxidase was at the latest stages of biodegradation. These enzyme secretions in distinct ratios and consequences are necessary for effective phenol biodegradation. An effective approach for phenol concentration decrease in the waste water of smoking shops in meat-processing factories using L. tigrinus fungus was described. 相似文献
7.
D. A. Kadimaliev V. V. Revin V. V. Shutova V. D. Samuilov 《Applied Biochemistry and Microbiology》2004,40(1):49-52
Changes in the chemical composition of cotton plant stems used as a substrate for solid-phase cultivation of the fungus Panus tigrinus were studied, as well as the effect of these changes on properties of pressed materials made of these stems. During the first 3 days of growth, the fungus better consumed cellulose; then, the rate of cellulose consumption was comparable with that of lignin. The intensity and pattern of these changes depended on the age of the inoculum. The rate of cotton plant waste biodegradation was higher when a 3-day-old inoculum was used. Pressed materials made of the raw material treated with a 3-day-old inoculum of P. tigrinus for 2–3 days displayed better characteristics. 相似文献
8.
Lipid composition, intracellular products of lipid peroxidation (LPO), and the activities of extracellular enzymes were studied during submerged cultivation of the xylotrophic fungus Lentinus (Panus) tigrinus VKM F-3616D. The maximum secretion of ligninolytic enzymes during the phase of active mycelium growth correlated with increased content of readily oxidized phospholipids and unsaturated fatty acids and with low content of the LPO products. In the idiophase, which was characterized by lower excretion of extracellular ligninolytic enzymes, the content of more stable phospholipids, saturated fatty acids, and LPO products increased. A relationship between the composition of mycelial lipids and the secretion of ligninolytic enzymes was revealed. 相似文献
9.
Lignin Degradation and Modification by the Soil-Inhabiting Fungus Fusarium proliferatum 总被引:2,自引:0,他引:2 下载免费PDF全文
V. Regalado A. Rodriguez F. Perestelo A. Carnicero G. De La Fuente M. A. Falcon 《Applied microbiology》1997,63(9):3716-3718
A soil-inhabiting Fusarium proliferatum strain was capable of transforming or degrading nonlabeled and (sup14)C-labeled industrial, natural, and synthetic lignin. The mineralization rate per day (expressed as the percentage of added radioactivity recovered as to (sup14)CO(inf2)) was maximal during primary metabolism. 相似文献
10.
Revin VV Cadimaliev DA Atykyan NA Sitkin BV Samuilov VD 《Biochemistry. Biokhimii?a》2000,65(11):1305-1309
Synthesis of peroxidase and laccase by the fungus Panus tigrinus was significantly stimulated by addition of the lignocellulose substrate to the culture media. Peroxidase was isolated from the culture liquid and some properties of the enzyme were investigated. P. tigrinus peroxidase belongs to a group of extracellular peroxidases similar to the plant type peroxidases. 相似文献
11.
Increased manganese concentration during submerged cultivation of the ligninolytic white rot fungus Panus tigrinus 8/18 on N-limited mineral medium resulted in the induction of Mn-peroxidase and laccase. The Mn-peroxidase was purified with the purity factor RZ (A
406/A
280) = 4.3. The purified enzyme catalyzed H2O2-dependent oxidation of phenol oxidase substrates (aromatic amines, 2,2"-azinobis-(3-ethylbenzthiazolinesulfonic acid), hydroquinone, 2,6-dimethoxyphenol) without Mn2+, which is not typical for the usual Mn-peroxidases. Guaiacol and 2,4,6-trichlorophenol were not oxidized in the absence of Mn2+. Study of absorption spectra of the intermediates of the catalytic cycle revealed that this peroxidase is able to complete the redox cycle, reducing one-electron oxidized intermediate (Compound II) by Mn2+, as well as by an organic substrate (hydroquinone). This means that the enzyme is a hybrid Mn-peroxidase, different from the common Mn-peroxidases from ligninolytic fungi. 相似文献
12.
Transformation of 2,4,6-trichlorophenol by the white rot fungi Panus tigrinus and Coriolus versicolor 总被引:7,自引:0,他引:7
The toxicity of thirteen isomers of mono-, di-, tri- and pentachlorophenols was tested in potato-dextrose agar cultures of the white rot fungi Panus tigrinus and Coriolus versicolor. 2,4,6-Trichlorophenol (2,4,6-TCP) was chosen for further study of its toxicity and transformation in liquid cultures of these fungi. Two schemes of 2,4,6-TCP addition were tested to minimize its toxic effect to fungal cultures: stepwise addition from the moment of inoculation and single addition after five days of growth. In both cases the ligninolytic enzyme systems of both fungi were found to be responsible for 2,4,6-TCP transformation. 2,6-Dichloro-1,4-hydroquinol and 2,6-dichloro-1,4-benzoquinone were found as products of primary oxidation of 2,4,6-TCP by intact fungal cultures and purified ligninolytic enzymes, Mn-peroxidases and laccases of both fungi. However, primary attack of 2,4,6-TCP in P. tigrinus culture was conducted mainly by Mn-peroxidase, while in C. versicolor it was catalyzed predominantly by laccase, suggesting a different mode of regulation of these enzymes in the two fungi. 相似文献
13.
《Biochemical Engineering Journal》2006,30(3):243-249
The white-rot fungus Panus tigrinus CBS 577.79 was cultivated both in mechanical (stirred tank, STR) and pneumatically (bubble column, BCB) agitated bioreactors and investigated for its ability to reduce the polluting load of olive-mill wastewater (OMW). Both aeration and agitation strongly influenced treatment efficiency. Best pollutants biodegradation performances were achieved in the bubble column bioreactor. Using this bioreactor, COD reduction, dephenolization and decoloration were 60.9, 97.2 and 75%, respectively. In contrast, lower depollution efficiency was generally observed in STR due to the possible occurrence of shear stress. 相似文献
14.
The possible use of olive-mill wastewater (OMW) as a growth medium for the production of extracellular laccase and manganese peroxidase (MnP) from the white-rot fungus Panus tigrinus (P. tigrinus) CBS 577.79 was studied using a properly formulated OMW-based medium (2-fold diluted OMW supplemented with 0.5% sucrose and 0.1% yeast extract) either in a stirred-tank or an air-lift reactor. Solid-state fermentation (SSF) was also performed in a rotary drum reactor using maize stalks moistened with the OMW-based medium. Highest levels of laccase and manganese peroxidase activity were obtained in the stirred-tank reactor (4600+/-98 U l(-1) on day 13) and in the air-lift reactor (410+/-22 on day 7), respectively. Based on total enzyme activities, SSF appears to be more suitable than LSF but the latter exhibits better volumetric productivities. 相似文献
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16.
Production of Wood Decay Enzymes,Loss of Mass,and Lignin Solubilization in Wood by Diverse Tropical Freshwater Fungi 总被引:1,自引:0,他引:1
In vitro production of cellulase and xylanase was common among diverse freshwater ascomycetes and their hyphomycetous anamorphs. Production of enzymes involved in lignin degradation was rare. Most isolates were capable of causing mass loss in angiosperm wood, although values were low, at ~10% during a 24-week period. A few isolates caused higher mass loss of up to 26.5%, and five of these were shown to solubilize significant amounts of lignin. This is the first report of lignin solubilization by freshwater fungi. Torula herbarum (hyphomycete) and Ophioceras dolichostomum (ascomycete) produced indices of lignin solubilization equivalent to those of terrestrial white-rot basidiomycetes. In all cases wood decay was 2.2- to 3-fold higher in exposed rather than submerged conditions. 相似文献
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18.
Influence of lignin and oxygen on the growth and the lipid formation of the fungus Lentinus tigrinus
A. A. Ivashechkin Ya. E. Sergeeva V. V. Lunin V. I. Bogdan I. S. Mysyakina E. P. Feofilova 《Applied Biochemistry and Microbiology》2014,50(3):286-291
During cultivation of the filamentous fungus Lentinus tigrinus on a medium containing lignin, a high oxygen content stimulated the growth of the fungus and contributed to the yield of lipids. A high content of phosphatidic acid and a reduction in the level of phosphatidylethanolamine and phosphatidylserine were first detected in the composition of phospholipids. Changes in the composition of neutral lipids, such as variation in the ratio of esterified and free sterols, have occurred; thus, the amount of sterol esters reduced simultaneously with a decrease in the content of free fatty acids. Based on the obtained results, the possible role of phosphatidic acid as a second messenger in the process of the consumption of lignin by the fungus Lentinus tigrinus is discussed. 相似文献
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20.
Kadimaliev DA Nadezhina OS Atykian NA Revin VV Parshin AA Lavrova AI Dukhovskis PV 《Prikladnaia biokhimiia i mikrobiologiia》2008,44(5):582-588
We studied the effects of butanol and toluene on secretion of lignolytic enzymes by the Lentinus tigrinus fungus during submerged cultivation. Addition of butanol and toluene during the trophophase was followed by an increase in laccase and peroxidase activity of the culture and change in the composition of phospholipids and fatty acids. The ratio of phosphatidylcholine and phosphatidic acid decreased, while the amount of lysophosphatidylcholine, phosphatidylethanolamine, phosphoinositides, phosphatidylserine, and unsaturated fatty acids decreased. These changes resulted in an increase in the unsaturation index. 相似文献