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1.
N. Yu. Markelova 《Microbiology》2010,79(6):777-779
Interaction of Bdellovibrio bacteriovorus 100NCJB with bacteria Campylobacter jejuni (strains 1, 2, 3, 4, and 5) and Helicobacter pylori, strain TX30a, was confirmed. The results indicate that lytic activity of bdellovibrios both in liquid media and cells attached
to a surface was observed. The potential use of the antimicrobial activity of predatory bacteria for environmental bioprotection
and public health is discussed. 相似文献
2.
There are three most important bacterial causative agents of serious infections that could be misused for warfare purposes:
Bacillus anthracis (the causative agent of anthrax) is the most frequently mentioned one; however, Fracisella tularensis (causing tularemia) and Yersinia pestis (the causative agent of plague) are further bacterial agents enlisted by Centers for Disease Control and Prevention into the category A of potential biological weapons. This review intends to summarize basic information about these bacterial
agents. Military aspects of their pathogenesis and the detection techniques suitable for field use are discussed. 相似文献
3.
V. Kumar D. Rao T. Thomas S. Kjelleberg S. Egan 《World journal of microbiology & biotechnology》2011,27(7):1543-1549
Bacteria and diatoms are primary colonizers of marine surfaces and hence play a crucial role in the attachment and subsequent
growth of macroorganisms. It has been suggested that the temperate green alga Ulva
lactuca relies on the defence provided by the epiphytic bacterial community to regulate surface fouling of colonising organisms.
In this study, ten resident bacterial isolates from tropical U. lactuca were tested for their antibacterial and antidiatom properties that may regulate surface colonization on the algae. Sixty
percent of the epiphytic isolates expressed antibacterial properties against other resident bacteria and 80% had antidiatom
activity against the pennate diatom, Cylindrotheca
fusiformis. Isolates of the Pseudoalteromonas genus showed both- antibacterial and antidiatom activities, while members of the genus Bacillus, Vibrio and Shewanella mostly possessed antidiatom activity. Our results show that a high proportion of bacterial isolates from tropical U. lactuca, like that of their temperate counterparts contain antibiotic properties that might impact on the bacterial community composition
and prevent fouling by diatoms. 相似文献
4.
Andrzej K. Siwicki Zdzisław Zakęś Elżbieta Terech-Majewska Krzysztof Kazuń Agnieszka Lepa Edward Głąbski 《Reviews in Fish Biology and Fisheries》2010,20(3):435-439
Influence of beta-1.3/1.6-glucan (Macrogard) on the innate immunity and protection against Aeromonas hydrophila in tench (Tinca tinca (L.)) was assessed. Macrogard was fed at doses of 0, 0.5, 1 and 2 g kg−1 of pellets for 1 month. The blood, spleen and head kidney from 10 fish of each group were separated and analysed for immunity
parameters. Twenty tench from each group were infected with A. hydrophila. Macrogard at doses 1 and 2 g kg−1 of feed significantly (P < 0.05) increased the phagocytic activity of macrophages and proliferative response of mitogens stimulated lymphocytes. The
same doses significantly (P < 0.05) increased lysozyme activity and Ig level in serum, compared to the control and dose 0.5 g kg−1 of feed. The challenge test showed that Macrogard reduced mortality of tench after experimental infection (5–35%). 相似文献
5.
O. V. Golovanova V. I. Konenkov A. V. Shevchenko M. V. Smolnikova 《Russian Journal of Genetics》2009,45(8):981-986
Based on population analysis of the DRB1, DQA1, DQB1 and TNFA allele frequency distribution patterns, regional features of immunogenetic structure of the population of West Siberia were
investigated. Statistically significant linkage disequilibrium within the HLA class II region, as well as between the TNFA and DRB1, DQA1, and DQB1 was demonstrated. Population frequency distribution patterns of two- and multilocus haplotypes were examined. 相似文献
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The intron sequence of chloroplast rpS16 and the secondary structure of its pre-mRNA were characterized for the first time in 26 Allium sativum accessions of different ecologo-geographical origins and seven related Allium species. The boundaries and main stem-loop consensus sequences were identified for all six domains of the intron. Polymorphism
was estimated for the total intron and its regions. The structural regions of the rpS16 intron proved to be heterogeneous for mutation rate and spectrum. Mutations were most abundant in domains II and IV, and
transition predominated in domains I, III, V, and VI. In addition to structural elements and motifs typical for group IIB
introns, several Allium-specific micro- and macrostructural mutations were revealed. A 290-bp deletion involving domains III and IV and part of domain
V was observed in A. altaicum, A. fistulosum, and A. schoenoprasum. Several indels and nucleotide substitutions were found to cause a deviation of the pre-mRNA secondary structure from the
consensus model of group II introns. 相似文献
9.
To overexpress the chitosanase gene (csn) in F. solani, a vector based on pCAMBIA 1300 was constructed. The csn gene, which is under control of the Aspergillus nidulans
gpdA promoter and A. nidulans trpC terminator, was introduced back into the F. solani genome by Agrobacterium tumefaciens-mediated transformation, and the herbicide-resistance gene bar from Streptomyces hygroscopicus was used as the selection marker. Transformants which showed a significant increase in chitosanase production (~2.1-fold
than control) were obtained. Southern blot analysis indicated that most transformants had a single-copy T-DNA integration. 相似文献
10.
Hiroyoshi Kubo 《Mycoscience》2009,50(5):400-406
Pilobolus crystallinus shows unique photoresponses at various growing stages. cDNAs for putative photoreceptors were cloned from this fungus. Three
genes named pcmada1, pcmada2, and pcmada3 were identified from the PCR fragments, and amplified with degenerated primers for the LOV domain, which is conserved in
many blue-light receptors. Deduced amino acid sequences for PCMADA1, PCMADA2, and PCMADA3 had one light-oxygen-voltage (LOV)-sensing
and two PER-ARNT-SIM (PAS) domains. A zinc finger DNA-binding motif was conserved in the C-terminals of PCMADA1 and PCMADA3.
However, PCMADA2 lacked the zinc finger motif. Expression of pcmada1 was suppressed by blue light whereas that of pcmada3 was promoted by blue-light irradiation. 相似文献
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Aeromonas hydrophila and A. salmonicida grown in pure cultures were found to secrete extracellular membrane nanovesicles into the environment. Outer membrane nanovesicle
preparations were isolated by differential centrifugation and ultrafiltration and visualized by transmission electron microscopy
applying the negative staining technique. Membrane nanovesicle size (10–300 nm) and ultrastructure were determined. The vesicles
were especially numerous around bacteria at the edge of small colonies. The process of the vesicle budding off the bacterial
cell was observed. On ultrathin sections of rat intestine, outer membrane nanovesicles were revealed among bacterial aggregates
of various species of parietal microorganisms. Short chains of such vesicles were also detected inside the glycocalyx between
the microvilli of the apical surface of the intestine epitheliocytes. On the basis of the results, together with the literature
data, the secretion of the outer membrane nanovesicles by pathogenic gram-negative bacteria, such as A. hydrophila and A. salmonicida, is proposed as a possible mechanism of pathogenesis leading to the host disease, as well as a means for cellular interactions
both within the prokaryote population and between the bacteria and the host organism. 相似文献
12.
Rosanna Capparelli Giorgia Borriello Romano Marabelli Sante Roperto Franco Roperto Domenico Iannelli 《Mammalian genome》2007,18(2):137-143
The 3′ untranslated region of the water buffalo Nramp1 (natural resistance-associated macrophage protein 1) gene contains two alleles (Nramp1A and Nramp1B), as detected by the denaturing gradient gel electrophoresis (DGGE) technique. The Nramp1BB genotype is associated with resistance of water buffalo to the intracellular pathogen Brucella abortus. This article provides evidence that the Nramp1AA genotype is associated with susceptibility to the same pathogen. Susceptibility or resistance of water buffalo to B. abortus was established by agglutination, complement fixation, and skin tests. The Nramp1 genotype was established by DGGE analysis. The association between the Nramp1AA genotype and susceptibility to B. abortus was demonstrated in two independent population samples (152 cases and 281 controls; 87 cases and 124 controls, respectively).
Macrophages from Nramp1AA subjects displayed a lower Nramp1 mRNA level when compared with macrophages from Nramp1BB subjects. Also, monocytes and macrophages from Nramp1AA subjects displayed a higher number of viable intracellular bacteria in comparison with monocytes and macrophages from Nramp1BB animals, providing biological significance to the results from association studies. 相似文献
13.
I. Rosario G. Soro S. Déniz O. Ferrer F. Acosta D. Padilla B. Acosta 《Mycopathologia》2010,169(4):315-319
Columba livia is an important reservoir and carrier of Cryptococcus neoformans, Cryptococcus uniguttulatus, Cryptococcus laurentii and Cryptococcus albidus. Upper digestive tract of this species is also known as a habitat for Cryptococcus neoformans. Given the increasing clinical interest of this microorganism, 331 swabs from crop and 174 dropping samples from pigeon lofts
in Grand Canary Island have been studied. The obtained results show an extensive presence samples 81 positive (24.47%) of
Cryptococcus spp. in analysed crops: 32 (9.66%) for C. neoformans, 24 (7.2%) for C. uniguttulatus, 23 (6.9%) for C. albidus and 2 (0.6%) for C. laurentii. In the same way, Cryptococcus spp was also isolated in 82 (47.13%), dropping samples: C. neoformans in 59 (33.9%), C. uniguttulatus, in 9 (5.17%), C. laurentii in 8 (4.59%) and C. albidus in 6 (3.44%) of the investigated samples, respectively. The cryptococcosis produced by species of cryptococci other than C. neoformans has become more important during the last decade, supporting the study on the role of pigeon in the epidemiology of this
disease. 相似文献
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Haoran An Hui Zhou Ying Huang Guohong Wang Chunguang Luan Jing Mou Yunbo Luo Yanling Hao 《Molecular biotechnology》2010,45(2):155-160
Lactic acid bacteria (LAB) are generally sensitive to hydrogen peroxide (H2O2), Lactobacillus sakei YSI8 is one of the very few LAB strains able to degrade H2O2 through the action of a heme-dependent catalase. Lactobacillus rhamnosus strains are very important probiotic starter cultures in meat product fermentation, but they are deficient in catalase. In
this study, the effect of heterologous expression of L. sakei catalase gene katA in L. rhamnosus on its oxidative stress resistance was tested. The recombinant L. rhamnosus AS 1.2466 was able to decompose H2O2 and the catalase activity reached 2.85 μmol H2O2/min/108 c.f.u. Furthermore, the expression of the katA gene in L. rhamnosus conferred enhanced oxidative resistance on the host. The survival ratios after short-term H2O2 challenge were increased 600 and 104-fold at exponential and stationary phase, respectively. Further, viable cells were 100-fold higher in long-term aerated cultures.
Simulation experiment demonstrated that both growth and catalase activity of recombinant L. rhamnosus displayed high stability under environmental conditions similar to those encountered during sausage fermentation. 相似文献
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Following a re-examination of the material treated under Barleria brevispina (Fiori) Hedrén in the recent Flora of Somalia account of the Acanthaceae, it is concluded that two distinct species are involved and Barleria compacta Malombe & I. Darbysh. is described here from north-eastern Somalia. Its affinities and conservation status are discussed. 相似文献
19.
Gaye Öngen Gaye Güngör Bahar Kanberoglu 《World journal of microbiology & biotechnology》2007,23(4):519-524
Aspergillus section Nigri strains Aspergillus aculeatus Ege-K 258, A.
foeditus var. pallidus Ege-K156, A. niger Ege-K 4 and A. tubingensis Ege-K 265 were used to treat olive mill wastewater (OMW) in an investigation aimed at exploring their dephenolisation and
decolourisation ability and, consequently, the economic feasibility of using any or all of these strains in a pre-treatment
step in the processing of OMW. Of these strains A. tubingensis Ege-K 265 resulted in an 80% decolourisation of twofold-diluted OMW and a 30% decolourisation of undiluted OMW; in addition,
it was able to remove approximately 30% of all phenolic compounds in both twofold-diluted and undiluted OMW. We conclude that
A. tubingensis Ege-K 265 could be effectively used in the pre-treatment step of a combined aerobic-anaerobic process to solve the environmental
problems caused by OMW in Mediterranean countries. 相似文献
20.
The life cycle and developmental stages of Aylax hypecoi (Trotter, 1913, Hymenoptera: Cynipidae: Aylacini) were studied in detail. Aylax hypecoi is known to induce galls in fruits of two Hypecoum species — H. imberbe and H. geslini (Papaveraceae) and the larva develops in host plant fruits. The morphology and development of egg, larva and pupa were investigated, which
has previously not been done. The shape and size of terminal-instar larvae and associated galls are sex-specific. Overwintering
stage, adult emergence and flying periods, and egg productivity were studied also. 相似文献