Dynamics of the oocyte cortical cytoskeleton during oogenesis in Rhodnius prolixus

The oocyte cortex undergoes dramatic changes during oogenesis in Rhodnius prolixus. Despite numerous studies examining oogenesis in the telotrophic ovariole, none has investigated the ultrastructural details of the oocyte cortex, in particular, the lateral cortical cytoskeleton. Indirect immunofluorescent staining of sections, rhodamine phalloidin staining of whole mounts and scanning and transmission EM of permeabilized and unpermeabilized preparations revealed the dynamic changes of the oocyte cortex from early previtellogenesis through to late vitellogenesis. During early previtellogenesis, oocytes 50-150 mum in length have a smooth oolemma, with no discernible cortical cytoskeleton. During mid to late previtellogenesis (oocytes 150-350 mum in length) a tightly woven network of microfilaments and microtubules forms, excluding mitochondria and Golgi complexes from the lateral cortex. At the onset of vitellogenesis, the follicuiar epithelium becomes patent, and there is an increase in microvilli covering the lateral oocyte surface. The microfilament cores form a discrete pattern that corresponds to the imprint of the follicle cells on the oocyte surface. While the lateral microfilament cytoskeleton becomes more elaborate, the lateral microtubule cytoskeleton diminishes, remaining sparse throughout vitellogenesis. The oocyte cortical cytoskeleton undergoes dramatic changes during oogenesis. These cortical dynamics are intricately related to the cellular and molecular processes that occur during oogenesis.     

Microtubules and Microfilaments in Amphibian Neurulation

In the salamander embryo, the morphogenetic movements of neurulationare correlated with two cell shape changes in the neural epithelium:elongation and apical constriction of the columnar neural platecells. Cells first elongate to form the flat open neural plateand then constrict apically as the plate rolls up to form theneural tube. Evidence is presented that these cell shape changesare intrinsic to the cells themselves and that they play a causalrole in the morphogenetic movements. Neural plate cells containnumerous microtubules oriented parallel to the axis of elongation.These microtubules are critical to the elongation process. Possiblemechanisms for microtubule function in cell elongation are considered.During apical constriction the cells contain bundles of microfilamentswhich encircle the cell apex in purse-string fashion. Evidenceis presented which suggests that microfilament bundles playan active role in apical constriction, and that this localizedcontraction is produced by filament sliding.     

A cytological study of the ovary of Rhodnius prolixus. I. The ontogeny of the follicular epithelium

The relatively undifferentiated cells comprising the prefollicular epithelium of the fourth and fifth instar of the reduvid bug Rhodninus prolixus are flattened and contain the regularly occurring organelles, lipid droplets, and aggregates of glycogen-like particles. These cells transform into the adult prefollicular tissue. During vitellogenesis there is a gradual shortening of the cells of the follicular epithelium and an increase in the size of the intercellular space between them and between follicle cells and oocyte. The follicle cells are binucleate, contain numerous microtubules, rough endoplasmic reticulum, many free and aggregate ribosomes, and Golgi complexes. They are associated with each other by gap junctions. Only the follicle cells on the lateral aspects of the oocyte exhibit the development of large extracellular spaces while those at the apical end, that produces the cap, remain tall and closely apposed to each other during vitellogenesis. The normal morphology of the follicle cells over various areas of the oocyte suggests that shape and/or volume changes of these cell may be important in regulating the access of yolk proteins to the colemma. Subsequent to vitellogenesis the follicle cells become cuboidal and once again become closely apposed to each other. They contain much rough endoplasmic reticulum and produce the secondary coat.     

Program of F-actin in the follicular epithelium during oogenesis of the german cockroach, Blattella germanica

Extensive programmed structural and functional changes of insect follicular epithelium during oogenesis provide a model to study modulation of cytoskeletal organization during morphogenesis in a non-dividing cell population. Rhodamine-phalloidin staining of whole mounted and cryosectioned oogenic follicles reveal changing F-actin filament organization from pre- to post-vitellogenic stages consistent with the presumptive dorsal-ventral orientation of the future embryo. Filaments are not abundant in pre-vitellogenic follicle cells up to day 2. Differences between dorsal and ventral follicle cells appear first on day 3. Obviously patent follicle cells are seen only on the ventral follicle surface which exhibits stronger F-actin fluorescence than the dorsal non-patent epithelium. On the presumptive ventral side of midvitellogenic follicles morphologically distinct bundles of actin filaments orient peripherally into projections connecting adjacent follicle cells and from the center of follicle cells apically into macrovillar projections extending toward the oocyte surface. The mid-vitellogenic dorsal follicle cell layer also possesses macrovillar extensions containing F-actin which reach and appear to penetrate the oolema. During chorion deposition major reorganization of actin of follicle cells takes place. After chorion deposition all F-actin filaments within a given follicle cell are arranged into large parallel bundles with semi-regular cross-striations which exclude fluorescent label. The parallel orientation of actin striated filament bundles within each follicle cell appears to be random with respect to the orientation of bundles in neighboring follicle cells over much of the mid-latitude of the follicle epithelium. At anterior and posterior follicle poles a more axial orientation of striated bundles is evident. This muscle-like tissue arrangement is appropriate for cooperation in ovulating the chorionated oocyte from the follicle into the oviduct.     

Ultrastructural modifications of the follicular epithelium accompanying the onset of vitellogenesis in the whirligig beetle,Gyrinus natator

Summary The ultrastructure of the follicle cells during previtellogenesis and early vitellogenesis have been studied. In previtellogenesis follicle cells are columnar with numerous bundles of microtubules located along the lateral plasma membranes. Oocyte-follicle cell gap junctions are not found in this stage. At the onset of vitellogenesis, the bundles of microtubules disappear and are replaced by an apically located ring of microtubules. The modification of microtubular cytoskeleton is not followed by the development of intercellular spaces between the follicle cells. Concurrently, numerous gap junctions are formed between specialized follicle cell processes and oocyte microvilli, which are arranged in characteristic cone-shaped aggregations. It is suggested that cytoskeletal changes and formation of heterologous gap junctions, occurring at the onset of vitellogenesis, are induced by juvenile hormone.     

The histological and histochemical studies on the ovary in relation to vitellogenesis in the dragonfly, Orthetrum chrysis Selys (Libellulidae: Odonata).

The ovaries consist of large number of panoistic ovarioles in the last instar nymph and the adult dragonfly Orthetrum chrysis (Selys). In the nymph the vitellaria are compactly filled with the primary oocytes and the vitellogenesis takes place only in the adult stage. During vitellogenesis oocytes change widely in their shape, size and cytological organisation and their developmental stages can be divided into pre-vitellogenic, early-vitellogenic, vitellogenic, late-vitellogenic and maturation age. PAS-positive material appears first around the germinal vesicle in the early-vitellogenic stage and lateron it migrates towards the periphery. Glycogen appears in the late-vitellogenic stage. DNA is abundantly present in the nuclei of the oocytes during the pre-vitellogenic and completely absent in early-vitellogenic, vitellogenic, late-vitellogenic and maturation stages. It is observed in the nuclei of follicular epithelial cells of all the stages. RNA is abundantly present in cytoplasm of the pre-vitellogenic oocytes but lateron is gradually decreases. During the early-vitellogenic and vitellogenic stages high concentration of RNA in the follicular epithelial cells has been observed. The protein bodies appear first in the interfollicular spaces and towards the periphery of the oocytes just near the enveloping follicular epithelial cells, during the early-vitellogenic stage suggesting the formation of yolk proteins from the haemolymph. In Orthetrum chrysis the sudanophilic bodies appear first in the follicular cells and then lie in the peripheral region of the oocytes suggesting the incorporation of yolk lipid either from the follicular epithelium or from the haemolymph through the follicular epithelium. The phospholipids are synthesised in pre-vitellogenic to the late-vitellogenic stages. In the late-vitellogenic stages the phospholipid granules are present abundantly in the follicular epithelium while in the maturation stage they disappear suggesting their utilisation in the formation of membranes like vitelline and chorion. The neutral fats are present in the form of large number of droplets in the oocytes during the maturation stage.     

Ovarian maturation and oogenesis in the blue swimmer crab,Portunus pelagicus (Decapoda: Portunidae)

The study was aimed at understanding the process of reproduction and the changes happening in the ovary of Portunus pelagicus during maturation, which would be useful for its broodstock development for hatchery purposes. For that, tissue samples from different regions of the ovary at various stages of maturation were subjected to light and electron microscopy, and based on the changes revealed and the differences in ovarian morphology, the ovary was divided into five stages such as immature (previtellogenic oocytes), early maturing (early vitellogenic oocytes), late maturing (late vitellogenic oocytes), mature (vitellogenic oocytes), and spent (resorbing oocytes). The ovarian wall comprised of an outermost thin pavement epithelium, a middle layer of connective tissue, and an innermost layer of germinal epithelium. The oocytes matured as they moved from the centrally placed germinal zone toward the ovarian wall. The peripheral arrangement of nucleolar materials and the high incidence of cell organelles during the initial stages indicated vitellogenesis I. Movement of follicle cells toward oocytes in the early maturing stage and low incidence of mitochondria and endoplasmic reticulum in the ooplasm during late vitellogenic stage marked the commencement and end of vitellogenesis II, respectively. Yolk granules at various stages of development were seen in the ooplasm from late vitellogenic stage onwards. The spent ovary had an area with resorbing oocytes and empty follicle cells denoting the end of one reproductive cycle and another area with oogonial cells and previtellogenic oocytes indicating the beginning of the next.     

Molecular mechanisms of cell shape changes that contribute to vertebrate neural tube closure

During early development of the central nervous system, the neuroepithelial cells undergo dynamic changes in shape, cumulative action of which cause the neural plate to bend mediolaterally to form the neural tube. The apicobasal elongation changes the cuboidal cells into columnar ones, whereas apical constriction minimizes the cell apices, causing them to adopt wedge-like shapes. To achieve the morphological changes required for the formation of a hollow structure, these cellular changes must be controlled in time and space. To date, it is widely accepted that spatial and temporal changes of the cytoskeletal organization are fundamental to epithelial cell shape changes, and that noncetrosomal microtubules assembled along apicobasal axis and actin filaments and non-muscle myosin II at the apical side are central machineries of cell elongation and apical constriction, respectively. Hence, especially in the last decade, intracellular mechanisms regulating these cytoskeletons have been extensively investigated at the molecular level. As a result, several actin-binding proteins, Rho/ROCK pathway, and cell-cell adhesion molecules have been proven to be the central regulators of apical constriction, while the regulatory mechanisms of cell elongation remain obscure. In this review, we first describe the distribution and role of cytoskeleton in cell shape changes during neural tube closure, and then summarize the current knowledge about the intracellular proteins that directly modulate the cytoskeletal organization and thus the neural tube closure.     

Structural organization of ovarian follicle cells in the cotton bug (Dysdercus intermedius) and the honeybee (Apis mellifera)

Summary The somatic epithelia of Dysdercus and Apis follicles were analyzed by electron microscopy, and the patterns of F-actin and microtubules were studied by fluorescence microscopy. The epithelia in both species differ considerably in shape and in the organization of the cytoskeleton. During previtellogenic stages, the epithelium consists of columnar-shaped cells with small (Dysdercus) or no (Apis) lateral intercellular spaces. During vitellogenesis, the follicle cells round up; the intercellular spaces increase in size in Dysdercus follicles, whereas in Apis follicles they remain small. Along the basal surface of the follicle cells, there are conspicuous parallel bundles of microfilaments perpendicular to the anteroposterior axis of the follicles. In the honeybee, these microfilament bundles are present in long filopodia, most of which are embedded in thickenings of the basement membrane and extend over the surfaces of neighbouring cells. In the cotton bug, the basal surface of the follicle cells is thrown into parallel folds. The microfilament bundles are located just underneath the cell membrane where the folds contact the basement membrane. In the polar regions of the Dysdercus follicle, the epithelial cells become flat and adhere to each other without forming intercellular spaces. The basement membrane is particularly thick in the polar areas; this has also been observed in Apis follicles around the intercellular bridge connecting oocyte and nurse cells.     

Apical spectrin is essential for epithelial morphogenesis but not apicobasal polarity in Drosophila.

Changes in cell shape and position drive morphogenesis in epithelia and depend on the polarized nature of its constituent cells. The spectrin-based membrane skeleton is thought to be a key player in the establishment and/or maintenance of cell shape and polarity. We report that apical beta(Heavy)-spectrin (beta(H)), a terminal web protein that is also associated with the zonula adherens, is essential for normal epithelial morphogenesis of the Drosophila follicle cell epithelium during oogenesis. Elimination of beta(H) by the karst mutation prevents apical constriction of the follicle cells during mid-oogenesis, and is accompanied by a gross breakup of the zonula adherens. We also report that the integrity of the migratory border cell cluster, a group of anterior follicle cells that delaminates from the follicle epithelium, is disrupted. Elimination of beta(H) prevents the stable recruitment of alpha-spectrin to the apical domain, but does not result in a loss of apicobasal polarity, as would be predicted from current models describing the role of spectrin in the establishment of cell polarity. These results demonstrate a direct role for apical (alphabeta(H))(2)-spectrin in epithelial morphogenesis driven by apical contraction, and suggest that apical and basolateral spectrin do not play identical roles in the generation of apicobasal polarity.     

Apical surface of hydrozoan nematocytes: Structural adaptations to mechanosensory and exocytotic functions

At the apical cell pole of the nematocytes of a hydrozoan (Hydra vulgaris), cytoskeletal elements of different molecular families (microfilaments, microtubules, cross-striated rootlets) are combined in a complex framework. As determined by transmission and scanning electron microscopy, the stimulus-transducing cnidocil apparatus of these mechanosensitive cells is forced into a lateral position, facilitating the close apposition of the nematocyst to the apical cell membrane. The nematocyst, a single, extraordinarily large, exocytotic organelle, is held in position by a microtubular basket. The cnidocil apparatus and microtubular basket are linked to an ellipsoid arrangement of pseudovilli, i.e., small surface protrusions containing cross-striated rods. These nematocyte-specific, cytoskeletal elements mediate the anchorage of the entire cytoskeletal framework to the apical cell membrane. The apical membranes of the nematocyte and nematocyst are separated by a distance of only ～ 50 nm. Structural modifications on the external side of the cyst membrane resemble those of synaptic membranes. © 1994 Wiley-Liss, Inc.     

Confocal scanning laser microscopy of the follicular epithelium in ovarioles of the stick insect Carausius morosus

Ovarian follicles of the stick insect Carausius morosus were analyzed by confocal laser microscopy and immunocytochemistry with a view to studying cell polarity in the follicular epithelium. Such probes as anti-α-tubulin antibodies and Rh-phalloidin were employed to establish how the follicle cell cytoskeleton changes during ovarian development. Data show that α-tubulin prevails over the basal end, while F-actin appears more abundant along the apical end of the follicle cells. This finding was further corroborated by immunogold cytochemistry, showing that label along the basal end is primarily associated with microtubules, while that along the apical end is due to follicle cell microvilli interdigitating with the oocyte plasma membrane. A monoclonal antibody specifically raised against a vitellin polypeptide was used to investigate the role the follicular epithelium might play in relation to vitellogenin (Vg) uptake by the oocyte. Data show that under these conditions label is restricted to the intercellular channels of the follicular epithelium, thus providing further support to the notion that Vg enters the oocyte through the extracellular pathway leading from the basement lamina to the oocyte surface. By contrast, the use of a monoclonal antibody raised against a fat-body-derived protein of 85 kDa that is specifically sulfated within the follicle cells provides evidence for the existence of an alternative way of gaining access to the oocyte surface, that is by transcytosis through the follicular cell epithelium. These findings confirm our earlier observations on stick insect ovarioles whereby polarization in the follicular epithelium is primarily addressed to sustain a transcytotic vesicular traffic between opposite poles of the follicle cell of Vg toward the oocyte surface.     

Sensitivity of Fibroblasts and Their Cytoskeletons to Substratum Topographies: Topographic Guidance and Topographic Compensation by Micromachined Grooves of Different Dimensions

Fibroblasts alter their shape, orientation, and direction of movement to align with the direction of micromachined grooves, exhibiting a phenomenon termed topographic guidance. In this study we examined the ability of the microtubule and actin microfilament bundle systems, either in combination with or independently from each other, to affect alignment of human gingival fibroblasts on sets of micromachined grooves of different dimensions. To assess specifically the role of microtubules and actin microfilament bundles, we examined cell alignment, over time, in the presence or absence of specific inhibitors of microtubules (colcemid) and actin microfilament bundles (cytochalasin B). Using time-lapse videomicroscopy, computer-assisted morphometry and confocal microscopy of the cytoskeleton we found that the dimensions of the grooves influenced the kinetics of cell alignment irrespective of whether cytoskeletons were intact or disturbed. Either an intact microtubule or an intact actin microfilament-bundle system could produce cell alignment with an appropriate substratum. Cells with intact microtubules aligned to smaller topographic features than cells deficient in microtubules. Moreover, cells deficient in microtubules required significantly more time to become aligned. An unexpected finding was that very narrow 0.5-μm-wide and 0.5-μm-deep grooves aligned cells deficient in actin microfilament bundles (cytochalasin B-treated) better than untreated control cells but failed to align cells deficient in microtubules yet containing microfilament bundles (colcemid treated). Thus, the microtubule system appeared to be the principal but not sole cytoskeletal substratum-response mechanism affecting topographic guidance of human gingival fibroblasts. This study also demonstrated that micromachined substrata can be useful in dissecting the role of microtubules and actin microfilament bundles in cell behaviors such as contact guidance and cell migration without the use of drugs such as cytochalasin and colcemid.     

Orientation of spindle axis and distribution of plasma membrane proteins during cell division in polarized MDCKII cells

MDCKII cells differentiate into a simple columnar epithelium when grown on a permeable support; the monolayer is polarized for transport and secretion. Individual cells within the monolayer continue to divide at a low rate without disturbing the function of the epithelium as a barrier to solutes. This presents an interesting model for the study of mitosis in a differentiated epithelium which we have investigated by confocal immunofluorescence microscopy. We monitored the distribution of microtubules, centrioles, nucleus, tight junctions, and plasma membrane proteins that are specifically targeted to the apical and basolateral domains. The stable interphase microtubule cytoskeleton was rapidly disassembled at prophase onset and reassembled at cytokinesis. As the interphase microtubules disassembled at prophase, the centrioles moved from their interphase position at the apical membrane to the nucleus and acquired the ability to organize microtubule asters. Orientation of the spindle parallel to the plane of the monolayer occurred between late prophase and metaphase and persisted through cytokinesis. The cleavage furrow formed asymmetrically perpendicular to the plane of the monolayer initiating at the basolateral side and proceeding to the apical domain. The interphase microtubule network reformed after the centrioles migrated from the spindle poles to resume their interphase apical position. Tight junctions (ZO-1), which separate the apical from the basolateral domains, remained assembled throughout all phases of mitosis. E-cadherin and a 58-kD antigen maintained their basolateral plasma membrane distributions, and a 114- kD antigen remained polarized to the apical domain. These proteins were useful for monitoring the changes in shape of the mitotic cells relative to neighboring cells, especially during telophase when the cell shape changes dramatically. We discuss the changes in centriole position during the cell cycle, mechanisms of spindle orientation, and how the maintenance of polarized plasma membrane domains through mitosis may facilitate the rapid reformation of the polarized interphase cytoplasm.     

A seasonal cycle of cell wall structure is accompanied by a cyclical rearrangement of cortical microtubules in fusiform cambial cells within taproots of Aesculus hippocastanum (Hippocastanaceae)

Aspects of the structure and ultrastructure of the fusiform cambial cells of the taproot of Aesculus hippocastanum L. (horse chestnut) are described in relation to the seasonal cycle of cambial activity and dormancy. Particular attention is directed at cell walls and the microtubule and microfilament components of the cytoskeleton, using a range of cytochemical and immunolocalization techniques at the optical and electron-microscopical levels. During the dormant phase, cambial cell walls are thick and multi-layered, the cells possess a helical array of cortical microtubules, and microfilament bundles are oriented axially. In the early stages of reactivation, vesicle-like profiles are associated with the cell walls, whereas arrangement of the cytoskeletal elements remains unchanged. In the succeeding active phase, the cell walls are thin, and cortical microtubules form a random array, although microfilament bundles maintain a near-axial orientation. The observations are discussed in relation to the seasonal cycle of wall structure and cortical microtubule rearrangement within the vascular cambium of hardwood trees. It is suggested that the cell-wall thickening at the onset of cambial dormancy, which is associated with the presence of a helical cortical microtubule array, should be considered to be secondary wall thickening, and that selective lysis of this secondary wall layer during cambial reactivation restores the thinner, primary wall found around active cambial cells.     

Ultrastructure of the choroid plexus epithelium of pigeons treated with drugs: II. Effect of cytochalasin D and colchicine

A remarkable projection of bleblike protrusions, the expulsion of organelles into the protrusions formed on the apical surface, and the separation into the ventricular lumen of these protrusions was the general cellular response of choroidal epithelial cells to intravenous injection of cytochalasin D (CD). The compact microfilament mass and agglomeration of microtubules at the base of the cluster of protrusions reflect the results of cell contraction and displacement of microfilaments induced by CD. In earlier stages after intravenous injections of colchicine, an obvious increase in the number of various-sized vesicles, vacuoles, and lysosomes in the Golgi region was detected. In the later stages, these organelles were seen to accumulate in the basal portion of the epithelial cells. These changes were accompanied by an increase in vacuoles and the disorganization and displacement of the Golgi complex, and they coincided with a decrease in the number of microtubules in apical and basal cytoplasm. These findings suggest that the action of colchicine results in destruction of the three-dimensional architecture between cytoskeletal network and cell organelles. The present results suggest that the cytoskeletal network plays a role in the spatial coordination of the three-dimensional architecture of cell organelles. The study also indicates that the structural differences in the ventricles of the choroid plexus in drug-treated pigeons are manifestations of regional functional specialization in different parts of the ventricular system.     

Caenorhabditis elegans morphogenesis: the role of the cytoskeleton in elongation of the embryo

During development Caenorhabditis elegans changes from an embryo that is relatively spherical in shape to a long thin worm. This paper provides evidence that the elongation of the body is caused by the outermost layer of embryonic cells, the hypodermis, squeezing the embryo circumferentially. The hypodermal cells surround the embryo and are linked together by cellular junctions. Numerous circumferentially oriented bundles of microfilaments are present at the outer surfaces of the hypodermal cells as the embryo elongates. Elongation is associated with an apparent pressure on the internal cells of the embryo, and cytochalasin D reversibly inhibits both elongation and the increase in pressure. Circumferentially oriented microtubules also are associated with the outer membranes of the hypodermal cells during elongation. Experiments with the microtubule inhibitors colcemid, griseofulvin, and nocodazole suggest that the microtubules function to distribute across the membrane stresses resulting from microfilament contraction, such that the embryo decreases in circumference uniformly during elongation. While the cytoskeletal organization of the hypodermal cells appears to determine the shape of the embryo during elongation, an extracellular cuticle appears to maintain the body shape after elongation.     

Vacuolar and cytoskeletal dynamics during elicitor-induced programmed cell death in tobacco BY-2 cells

Responses of plant cells to environmental stresses often involve morphological changes, differentiation and redistribution of various organelles and cytoskeletal network. Tobacco BY-2 cells provide excellent model system for in vivo imaging of these intracellular events. Treatment of the cell cycle-synchronized BY-2 cells with a proteinaceous oomycete elicitor, cryptogein, induces highly synchronous programmed cell death (PCD) and provide a model system to characterize vacuolar and cytoskeletal dynamics during the PCD. Sequential observation revealed dynamic reorganization of the vacuole and actin microfilaments during the execution of the PCD. We further characterized the effects cryptogein on mitotic microtubule organization in cell cycle-synchronized cells. Cryptogein treatment at S phase inhibited formation of the preprophase band, a cortical microtubule band that predicts the cell division site. Cortical microtubules kept their random orientation till their disruption that gradually occurred during the execution of the PCD twelve hours after the cryptogein treatment. Possible molecular mechanisms and physiological roles of the dynamic behavior of the organelles and cytoskeletal network in the pathogenic signal-induced PCD are discussed.Key words: actin microfilament, cell cycle, cryptogein, microtubules, nuclei, programmed cell death, tobacco BY-2 cells, vacuoles     

A primary role for microfilaments, but not microtubules, in hormone-induced cytoplasmic retraction

The distributions of microfilaments and microtubules were studied during transient hormone-induced changes in cell shape (retraction-respreading). Two cell types (fibroblasts and bone cells), differentially responsive to parathyroid hormone (PTH) and prostaglandin E₂ (PGE₂), were analysed. The cytoplasm of fibroblasts retracted in response to PGE₂ but not PTH, whereas bone cells could respond to both PGE₂ and PTH. Time-lapse photomicrography indicated that the retraction began within minutes of hormone addition, while respreading occurred over longer times, up to 8 h. Affinity-purified actin and tubulin antibodies were used to follow the appearance of microtubules and microfilaments during both the retraction and the respreading phases. Microtubules appeared not to reorganize noticeably, although they were squeezed closer together in cellular pseudopods; no extensive loss or growth was detectable. Microfilaments did alter drastically their appearance and distributions. Soon after hormone addition when earliest detectable cytoplasmic retraction was evident, microfilament bundles appeared to break down. Remaining microfilament bundles consisted of relatively short, non-aligned fragments or aggregates. During respreading, microfilament bundles regrew and realigned throughout the cytoplasm. These data suggest a primary role for microfilaments, but probably not microtubules, in these cell shape changes.     

九孔鲍卵子发生及卵巢发育的组织学观察

采用组织学方法研究了九孔鲍(Haliotis diversicolor supertexta)的卵子发生、卵巢结构及其发育.根据卵细胞的大小、形状,核仁的形态,卵黄颗粒的积累情况,滤泡的结构等.将九孔鲍卵子的发生分为卵原细胞、卵黄发生前的卵母细胞和卵黄发生期的卵母细胞3个时期;卵巢壁由外膜及内生殖上皮构成,生殖上皮分化产生卵原细胞和滤泡细胞;卵巢的结构单位是滤泡.根据卵巢的外部形态和内部组织结构,将九孔鲍的卵巢发育分为休止期、增殖期、生长期、成熟期和排放期共5期.