Athenolide A,a New Steroidal Lactone from the Leaves of Athenaea martiana (Solanaceae) Determined by Means of HPLC‐HR‐MS‐SPE‐NMR Analysis

Athenaea (Solanaceae) is an endemic genus belonging to the Brazilian Atlantic Rainforest. Recently, botanical investigations suggested the re‐evaluation of the generic status of the genus Athenaea as a synonym of Aureliana. In this study, the first investigation of the Athenaea genus performed on Athenaea martiana by means of HPLC‐HR‐MS‐SPE‐NMR combined with high‐resolution radical scavenging profile led to identification of several phenolic acids as radical scavengers: protocatechuic acid ( 1 ), 4‐hydroxybenzoic acid ( 2 ), caffeic acid ( 3 ), vanillic acid ( 4 ), and ferulic acid ( 6 ). Additional analysis revealed a new steroidal lactone, named athenolide A ( 9 ). Their structures were elucidated by extensive use of NMR spectroscopy as well as HR‐MS. Chemotaxonomic considerations based on these results supported the chemical relationships between the Athenaea and Aureliana genera, in agreement with the recent botanical findings.     

Antifungal effects of selected botanicals on fungal pathogens of watermelon fruit

This study investigates the use of botanical extracts (Piper guineense, Xylopia aethiopica and Bambusa vulgaris at 5, 10 and 15% concentration levels) for controlling deteriorating fungal pathogens in vitro. Identification of the fungal pathogens after pathogenicity test reaffirm isolates identity as Fusarium oxysporum, Fusarium solani, Macrophomina phaseolina, Fusarium verticillioides and Botryodiplodia theobromae. All the fungal pathogens induced rot in the watermelon fruit. The botanical extracts were best effective at high concentration levels (10 and 15%), though varied in their inhibitory activity. P. guineense and X. aethiopica were observed to be more effective than B. vulgaris. This study showed the efficacy of the botanical extracts on deteriorating fungal pathogens of watermelon fruit. Thus, the botanical extracts can be employed as antideteriorating biological-based fungicides for watermelon fruits.     

Lineamenti della vegetazione lucana

Abstract

The vegetation of Lucania: an outline. – The vegetation of Lucania is outlined on the basis of the existing literature and of personal observations. After a short review of the botanical literature a new subdivision of this region is proposed taking into consideration both geological and botanical criteria. The various plant formations are described, among them: the vegetation of the sandy beaches along the Ionian Sea; the Policoro Forest on the coastal plain; the Lygeum spartum stands on the clays; the Quercus cents forests, and the Fagus silvatica ones (comprising the two associations Aquifolio-Fagetum and Asyneumati-Fagetum); the relict Abies alba stands; the summit prairies; the Pinus leucodermis stands.     

Araceae of Mulu National Park. I. Four new species of Schismatoglottis (Araceae)

Four new species of Schismatoglottis are described and illustrated from Mulu National Park in Malaysian Borneo: Schismatoglottis fossae (Patentinervia Clade), S. roseopedes and S. serratodentata (Calyptrata Clade) and S. pellucida (currently unplaced but likely in the Multinervia clade). A brief summary is provided of botanical exploration at Mulu, notably where pertinent to aroid botany.     

Four new species of <Emphasis Type="Italic">Phyllosticta</Emphasis>, one new species of <Emphasis Type="Italic">Pseudocercospora</Emphasis>, and one new combination in <Emphasis Type="Italic">Passalora</Emphasis> from Japan

During a survey of plant-inhabiting fungi in a botanical garden in Japan, some noteworthy fungi were collected from leaf spots of some herbal and arboreal plants. Among them, five new species are described, namely: Phyllosticta. ardisiicola on Ardisia crenata, Phy. aspidistricola on Aspidistra elatior, Phy. kerriae on Kerria japonica, Phy. fallopiae on Fallopia japonica, and Pseudocercospora davidiicola on Davidia involucrata. Passalora pyrrosiae, a new combination for Pseudocercospora pyrrosiae on Pyrrosia lingua, is proposed based on its morphological characteristics designating the neotype specimen.     

Plants with Histories: The Changing Ethnobotany of Iquito Speakers of the Peruvian Amazon

Plants with Histories: The Changing Ethnobotany of Iquito Speakers of the Peruvian Amazon. This paper describes the first ethnobotanical study carried out with speakers of Iquito, a critically endangered language of northeastern Peru. The work examines significant changes the pharmacopoeia has undergone, developing a new measure, the Index of Plant Novelty (IPN) that combines oral history, linguistic, comparative ethnobotanical, and botanical data. Research was carried out in 2009 and 2010, with structured interviews and collection of 87 botanical voucher specimens in 39 botanical families. The study results suggest that no one single factor can fully explain the patterns of borrowing in the pharmacopoeia of San Antonio, although data show some support for previously proposed hypotheses that such borrowing fills gaps in the pharmacopoeia or that edible and ornamental plants are often adopted for medicinal use. However, this process must also be understood within the context of colonization by mestizo settlers, which has given higher prestige to uses and species from outside. A comparison between oral history data and the linguistic and comparative data suggests that the Iquito informants interviewed have underestimated the extent to which their current pharmacopoeia has been borrowed.     

Numerical taxonomic studies of the genus Pyrus using both chemical and botanical characters*

Of three computer-generated classifications of Pyrus species, produced respectively from 29 chemical characters, 22 botanical characters and all 51 characters taken together, the classification based upon both chemical and botanical characters agrees best with the known geographical distributions of the species. Where either chemical or botanical characters are separately employed, the classifications contain seriously misplaced species. The numerical classification based upon all 51 characters lends some support to the division of the genus into four principal groups of species: the East Asian pea pears, the larger-fruited East Asian pears, the North African pears and the European and West Asian pears. P. longipes, P. betulifolia and P. cordata are considered as being connecting links between the East Asian pea pears, the larger-fruited East Asian pears and the European and West Asian pears. Some speculations are made on the phylogenetic relationships among Pyrus species.     

Two new species of Protium from French Guiana. Studies in neotropical Burseraceae VIII

Revision of Protium in French Guiana has revealed two new species. Protium gallicum is related to P. aracouchini (Aubl.) Marchand and P. elegans Engl., although its fruits resemble those of P. plagiocarpium Bénoist. Protium inodorum, which also occurs in northern Pará, Brazil, is related to P. pallidum Cuatrec. and P. cuneatum Swart; it is known only from flowers and very young fruits. Even in relatively well-known regions of tropical forest, botanical exploration continues to yield new taxa.     

中国兰科植物研究杂记

中国西藏墨脱县位于东喜马拉雅和印缅交界地区,是雅鲁藏布江大峡谷国家级自然保护区的核心区域,是喜马拉雅生物多样性热点地区之一。该文通过对中国西藏墨脱的科学考察,报道了中国兰科植物2个新记录种,即西藏牛角兰(Ceratostylis radiata)和格当石豆兰(Bulbophyllum psychoon),并提供了描述和图片。西藏牛角兰花为纯白色,花辐射对称,唇瓣3裂,茎长2~2.5 cm,明显区别于该属内其他种。格当石豆兰与齿瓣石豆兰(Bulbophyllum levinei)相似,但不同之处在于格当石豆兰的花瓣卵形,先端锐尖。凭证标本分别保存于西藏自治区高原生物研究所(XZ)和中国科学院植物研究所(PE)标本馆中。2个新记录种原分布均在印度、缅甸和越南等地区,在中国西藏的发现说明了中国西藏墨脱的植物区系和原分布地有一定的关系,同时也印证了中国西藏墨脱属于亚热带地区,且2个新记录种的分布海拔超出了原分布地范围,开花的时间也相对推迟。此新记录种的发现对摸清该区植物种类和丰富中国西藏植物区系提供了更加详实的资料。     

Foraging behaviour of the stingless bee Melipona orbignyi (Hymenoptera: Apidae: Meliponini) in a dry forest assessed by multivariate analysis from palynological data

Pollen analysis was applied to study the botanical composition of honey and pollen stored by Melipona orbignyi in its southernmost distributional range. Many studies have revealed that robust buzzing bees of this genus are associated with diverse plant families, some of which are different to those exploited by the remaining genera of Meliponini. Cluster analysis was performed to assess similarity in botanical composition between pot-pollen and pot-honey provisions from different colonies. The results indicated that each colony was capable to access to different plant species for obtaining nectar and pollen, a fact observed when discarding the contaminated honey samples. As honey samples were not clustered together with other ones but with pollen samples indicating a similar botanical composition, it could be assumed that a same plant species provided nectar and pollen to bees. Resource partitioning was not clearly observed among a subset of late spring colonies, as two families of high richness of species and abundant in this dry forest (the mimosoid clade in Fabaceae and Capparaceae) were foraged. A higher number of synchronously sampled colonies would be necessary to detect this ecological aspect. The botanical composition of samples of M. orbignyi was governed by both random factors such as local and temporary flower availability plus preferences for particular plant species such as those from Solanaceae and Ximeniaceae. The Capparaceae and Ximeniaceae are here reported for the first time as intensively foraged pollen resources by the genus Melipona.     

Report of the Alpine Section, 1958

Background: Over 10,000 island endemic angiosperms are highly threatened by extinction. Yet, few of these species have the temporal change in their range documented and quantified, particularly within a potentially informative context of a long period of botanical study.

Aim: Here, we used Roussea simplex a mono-specific genus endemic to Mauritius, itself an island with long botanical history and advanced habitat destruction extent, to investigate how the distribution and population of this model oceanic island plant changed through time.

Methods: All known localities and population size estimates were compiled from published literature, herbarium specimens, surveys and personal communications to estimate changes in population size, extent of occurrence and area of occupancy and investigate main distribution patterns.

Results: Roussea simplex survives in nine high elevation sites. Since the 1930s, its range halved relative to its maximum known distribution and its population size decreased much faster than direct habitat loss would predict. It now qualifies as Endangered according to the IUCN Red List categories.

Conclusions: Even in an extremely deforested island, endemic plant population decline may be driven more by diminishing habitat quality than diminishing habitat extent. This renders habitat protection alone insufficient, therefore addressing ecological interactions is vital to stem population decline.     

Classification of Modern Resins by Solid State Nuclear Magnetic Resonance Spectroscopy

The carbon-13 nuclear magnetic resonance (NMR) spectra of modern resins have been used to characterize their botanical sources. Resins from four of the principal diterpenoid resinproducing genera, Agathis, Hymenaea, Pinus, and Araucaria, give distinct NMR spectra under conditions of normal and interrupted decoupling. The genus Wollemia gives the same spectra as Agathis. Samples from the triterpenoid resin-producing family Burseraceae and of gum resins were recorded for comparison. Resin samples from unknown or uncertain sources then were examined and assigned to their botanical sources.     

Two new species of <Emphasis Type="Italic">Bertolonia</Emphasis> (Melastomataceae) from the Brazilian Atlantic Forest

Bertolonia alternifolia and B. bullata, two new endemic species of Melastomataceae from the Atlantic Forest biome of the southern region of Bahia state, Brazil, are described and illustrated based on recent botanical explorations and literature records. The main diagnostic characteristics that distinguish B. alternifolia are alternate leaves with cordate bases, and anthers with terminal-dorsal pores and unappendaged connectives. B. bullata can be identified by having leaves bullate on the adaxial surface, foveolate on the abaxial surface and bases cordate to cordate-lobate, calyx lobes with entire to slightly undulate margins, and stamens dorsally appendaged. Both species are classified as Critically Endangered (CR) and Endangered (EN) respectively.     

Comparative bioactivity of selected extracts from Meliaceae and some commercial botanical insecticides against two noctuid caterpillars, <Emphasis Type="Italic">Trichoplusia ni</Emphasis> and <Emphasis Type="Italic">Pseudaletia unipuncta</Emphasis>

Plant-derived extracts and phytochemicals have long been a subject of research in an effort to develop alternatives to conventional insecticides but with reduced health and environmental impacts. In this review we compare the bioactivities of some plant extracts with those of commercially available botanical insecticides against two important agricultural pests, the cabbage looper, Trichoplusia ni and the armyworm, Pseudaletia unipuncta. Test materials included extracts of Azadirachta indica (neem), A. excelsa (sentang), Melia volkensii, M. azedarach (Chinaberry) and Trichilia americana, (all belonging to the family Meliaceae) along with commercial botanical insecticides ryania, pyrethrum, rotenone and essential oils of rosemary and clove leaf. Most of the extracts and botanicals tested proved to be strong growth inhibitors, contact toxins and significant feeding deterrents to both lepidopteran species. However, there were interspecific differences with T. ni generally more susceptible to the botanicals than the armyworm, P. unipuncta. All botanicals were more inhibitory to growth and toxic (through feeding) to T. ni than to P. unipuncta, except for M. azedarach which was more toxic to P. unipuncta than to T. ni. Athough, pyrethrum was the most toxic botanical to both noctuids, A. indica, A. excelsa, and M. volkensii were more toxic than ryania, rotenone, clove oil and rosemary oil for T. ni. As feeding deterrents, pyrethrum was the most potent against T. ni, whereas A. indica was the most potent against the armyworm. Based upon growth inhibition, chronic toxicity, and antifeedant activity, some of these plant extracts have levels of activity that compare favorably to botanical products currently in commercial use and have potential for development as commercial insecticides.     

Insecticidal compound isolated from Syzygium lineare Wall. (Myrtaceae) against Spodoptera litura (Lepidoptera: Noctuidae)

A new crystal compound 2,5-diacetoxy-2-benzyl-4,4,6,6-tetramethyl-1,3-cyclohexanedione was isolated from the leaves of Syzygium lineare. The insecticidal activity of the compound was assessed against fourth instar larvae of Spodoptera litura. Its activity was better than the positive control azadirachtin. The compound was responsible for growth inhibition on S. litura. It induced larval, pupal and adult deformities even at low concentration. The compound may be useful as a botanical pesticide.     

A new enigmatic apterous cyclostome genus (Hymenoptera: Braconidae) from the Greek Islands

A new West Palaearctic genus Achaiabracon gen. n. with the type species A. aenigmatus sp. n. is described and illustrated from the Greek Islands Crete and Corfu. The position of this genus in the cyclostome group of subfamilies of Braconidae and its possible relation with rhyssaline genera are discussed. A new tribe Achaiabraconini trib. n. is proposed.     

“Candidatus Paraholospora nucleivisitans”, an intracellular bacterium in Paramecium sexaurelia shuttles between the cytoplasm and the nucleus of its host

An intracellular bacterium was discovered in two isolates of Paramecium sexaurelia from an aquarium with tropical fish in Münster (Germany) and from a pond in the Wilhelma zoological–botanical garden, Stuttgart (Germany). The bacteria were regularly observed in the cytoplasm of the host, but on some occasions they were found in the macronucleus of the host cell. In these cases, only a few, if any, bacteria were observed remaining in the cytoplasm. The bacterium was not infectious to P. sexaurelia or other species of Paramecium and appeared to be an obligate intracellular bacterium, while bacteria-free host cells were completely viable. The fluorescence in situ hybridisation (FISH) and comparative 16SrDNA sequence analyses showed that the bacterium belonged to a new genus, and was most closely, yet quite distantly, related to Holospora obtusa. In spite of this relationship, the new bacteria differed from Holospora by at least two biological features. Whereas all Holospora species reside exclusively in the nuclei of various species of Paramecium and show a life cycle with a morphologically distinct infectious form, for the new bacterium no infectious form and no life cycle have been observed. For the new bacterium, the name Candidatus Paraholospora nucleivisitans is suggested. The host P. sexaurelia is usually known from tropical and subtropical areas and is not a species typically found in Germany and central Europe. Possibly, it had been taken to Germany with fish or plants from tropical or subtropical waters. Candidatus Paraholospora nucleivisitans may therefore be regarded as an intracellular neobacterium for Germany.     

A reconsideration of the Lower Guinean species of Sericanthe (Rubiaceae,Coffeeae), with four new species from Cameroon and Gabon

The genus Sericanthe was established and its species were revised 30 years ago by one of the authors. Intensive recent botanical collecting in Lower Guinea (mainly southern Cameroon, Equatorial Guinea and Gabon) has made a reconsideration of species in this area necessary. Revision of the newly collected Lower Guinean herbarium specimens of Sericanthe (twice as many as were available at the time of the first revision) has brought four new species to light, namely S. gabonensis and S. mpassa from Gabon, S. lowryana from Cameroon and S. rabia from Cameroon and Gabon. Distributional data are much augmented, and distributional maps of all species from the area are provided. Four species were reported to be newly recorded for at least one country. The occurrence of the novelties in the main diversity centre of the genus is discussed in the context of the chorology of the entire genus Sericanthe. A first estimation of the conservation status for the new species is given. A synopsis of the genus Sericanthe in Lower Guinea with a taxonomic key is provided. © 2012 The Linnean Society of London, Botanical Journal of the Linnean Society, 2012, 169 , 530–554.