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Transcription of Simian virus 40 DNA in a HeLa whole cell extract 总被引:22,自引:0,他引:22
H Handa R J Kaufman J Manley M Gefter P A Sharp 《The Journal of biological chemistry》1981,256(1):478-482
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Faithful initiation of ribosomal RNA transcription from cloned DNA by purified RNA polymerase I 总被引:8,自引:0,他引:8
M R Paule C T Iida P J Perna G H Harris S L Brown Shimer P Kownin 《Biochemistry》1984,23(18):4167-4172
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Initiation of Genomic Plus-Strand RNA Synthesis from DNA and RNA Templates by a Viral RNA-Dependent RNA Polymerase 总被引:4,自引:0,他引:4
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In contrast to the synthesis of minus-strand genomic and plus-strand subgenomic RNAs, the requirements for brome mosaic virus (BMV) genomic plus-strand RNA synthesis in vitro have not been previously reported. Therefore, little is known about the biochemical requirements for directing genomic plus-strand synthesis. Using DNA templates to characterize the requirements for RNA-dependent RNA polymerase template recognition, we found that initiation from the 3' end of a template requires one nucleotide 3' of the initiation nucleotide. The addition of a nontemplated nucleotide at the 3' end of minus-strand BMV RNAs led to initiation of genomic plus-strand RNA in vitro. Genomic plus-strand initiation was specific since cucumber mosaic virus minus-strand RNA templates were unable to direct efficient synthesis under the same conditions. In addition, mutational analysis of the minus-strand template revealed that the -1 nontemplated nucleotide, along with the +1 cytidylate and +2 adenylate, is important for RNA-dependent RNA polymerase interaction. Furthermore, genomic plus-strand RNA synthesis is affected by sequences 5' of the initiation site. 相似文献
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