共查询到20条相似文献,搜索用时 31 毫秒
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Fuli Yu Jian Lu Xiaoming Liu Elodie Gazave Diana Chang Srilakshmi Raj Haley Hunter-Zinck Ran Blekhman Leonardo Arbiza Cris Van Hout Alanna Morrison Andrew D. Johnson Joshua Bis L. Adrienne Cupples Bruce M. Psaty Donna Muzny Jin Yu Richard A. Gibbs Alon Keinan Andrew G. Clark Eric Boerwinkle 《PloS one》2015,10(3)
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Cloning and expression profiling of testis-expressed microRNAs 总被引:2,自引:0,他引:2
Using a new small RNA cloning method, we identified 141 miRNAs from the mouse testis, of which 29 were novel. The 141 miRNAs were mapped onto all chromosomes except the Y chromosome and 2/3 of these miRNA genes exist as clusters. ∼ 70% of these miRNA genes were located in intronic or intergenic regions, whereas the remaining miRNAs were derived from exonic sequences. We further validated these cloned miRNAs by examining their expression in multiple mouse organs including developing testes and also in purified spermatogenic cells using semi-quantitative PCR analyses. Our expression profiling assays revealed that 60% of the testis-expressed miRNAs were ubiquitously expressed and the remaining are either preferentially (35%) or exclusively (5%) expressed in the testis. We also observed a lack of strand selection during testicular miRNA biogenesis, characterized by paired expression of both the 5′ strands and 3′ strands derived from the same precursor miRNAs. The present work identified numerous miRNAs preferentially or exclusively expressed in the testis, which would be interesting targets for further functional studies. 相似文献
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Arabidopsis transcriptome analysis under drought, cold, high-salinity and ABA treatment conditions using a tiling array 总被引:3,自引:0,他引:3
Matsui A Ishida J Morosawa T Mochizuki Y Kaminuma E Endo TA Okamoto M Nambara E Nakajima M Kawashima M Satou M Kim JM Kobayashi N Toyoda T Shinozaki K Seki M 《Plant & cell physiology》2008,49(8):1135-1149
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Bernard J. F. Perey 《CMAJ》1966,94(9):437-441
Injections of tetanus antitoxin of animal origin frequently cause serious disability and sometimes death. Despite world-wide knowledge of these effects, millions of prophylactic injections of equine tetanus antitoxin are given annually, and it is continually proposed that the dosage be increased in order to obtain higher “protective” levels in the serum, a procedure which would increase the incidence and severity of reactions. Furthermore, equine antitoxin frequently fails to prevent tetanus.Tetanus antitoxin of human origin is available which carries no risk of complications and confers a higher degree of immunity more quickly than equine antitoxin. The cost of treating reactions to horse serum, together with the financial loss incurred by work-absence, far outweighs the cost of human antitoxin. In the author''s opinion, the use, in this country, of antitoxin of animal origin is no longer medically acceptable and may well prove legally indefensible. 相似文献
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