A polymorphic microsatellite from the Squalius alburnoides complex (Osteichthyes, Cyprinidae) cloned by serendipity can be useful in genetic analysis of polyploids

A new microsatellite locus (SAS1) for Squalius alburnoides was obtained through cloning by serendipity. The possible usefulness of this new species-specific microsatellite in genetic studies of this hybrid-species complex, was explored. The polymorphism exhibited by SAS1 microsatellite is an important addition to the set of microsatellites previously used in genetic studies in S. alburnoides complex, that mostly relied in markers described for other species. Moreover, the SAS1 microsatellite could be used to identify the parental genomes of the complex, complementing other methods recently described for the same purpose..     

大叶藤黄叶片角质层的酶分离技术

角质层运输特征的研究在生态学、环境和农用化学等方面都具有非常重要的意义 ,完好地分离角质层是进行这方面研究的前提。本文以生长在西双版纳热带雨林中的大叶藤黄为材料 ,探讨了角质层的酶分离技术。结果表明 ,30℃时用 2 % (w/v) ,pH 3 0的果胶酶和纤维素酶溶液浸泡大叶藤黄叶圆片 9h ,叶圆片边缘角质层分离 ,浸泡 5d可分离到完整的角质层 ,角质层在硼酸盐缓冲液 (0 0 1mol·L-1,pH 9 0 )中充分浸泡数日去除石炭酸 ,空气中干燥 ,室温下于聚乙烯的盒子保存备用     

The efficacy and cardiac evaluation of aminomethyl tetrahydronaphthalene ketopiperazines: a novel class of potent MCH-R1 antagonists

The design, synthesis, and biological studies of a novel class of MCH-R1 antagonists based on an aminotetrahydronaphthalene ketopiperazine scaffold is described. Compounds within this class promoted significant body weight reduction in mouse diet induced obesity studies. The potential for hERG blockage activity and QT interval studies in anesthetized dogs are discussed.     

637. FRITILLARIA YUMINENSIS

Fritillaria yuminensis X.Z. Duan (Liliaceae) is described and illustrated; the species in the Fritillaria verticillata complex are listed and their relationship to the rest of the genus, as revealed by DNA studies, is described. The cultivation of Fritillaria (bei mu) for medicine and its conservation in China are summarised.     

A new, rapid, method for preparation of dispersed pancreatic acini.

A new method for the preparation of pancreatic acini is described. The method is simple and much more rapid than previously described techniques, the time required for preparation of pancreatic acini being 20 min from removal of the pancreas. Acini prepared with this method perform in a superior manner when stimulated by either caerulein or secretin. Thus this new technique would be ideal for use in binding and secretion studies.     

Determination of cocaine, benzoylecgonine and ecgonine methyl ester in plasma by reversed-phase high-performance liquid chromatography

A combined assay is described for cocaine and its major metabolites, benzoylecgonine and ecgonine methyl ester. The method uses electrochemical and ultraviolet detectors in series. A non-silica column is used with high-pH mobile phase. The three compounds are completely separated from other cocaine metabolites. The assay has been suitable for pharmacokinetic studies of cocaine disposition in animal studies.     

Integration of DNA ligation and rolling circle amplification for the homogeneous,end-point detection of single nucleotide polymorphisms

Association studies using common sequence variants or single nucleotide polymorphisms (SNPs) may provide a powerful approach to dissect the genetic inheritance of common complex traits. Such studies necessitate the development of cost-effective, high throughput technologies for scoring SNPs. The method described in this paper for the co-detection of both alleles of a SNP in a single homogeneous reaction combines the specificity of a high fidelity DNA ligation step with the power of rolling circle amplification. The incorporation of Amplifluor™ energy transfer primers enables signal detection in a homogeneous format, making this approach highly amenable to automation. The adaptation of the genotyping method for high throughput screening using conventional liquid handling systems is described.     

Observations on the life histories of Paramacroderoides pseudoechinus sp. n. (Digenea: Plagiorchioidea) and P. echinus Venard 1941, trematodes from the Florida gar.

Paramacroderoides pseudoechinus sp. n. is described from the intestine of Lepisosteus platyrhincus. Life history studies reveal that developmental stages previously reported for P. echinus from gars belong instead to the new species. The account of the life history of P. echinus is emended accordingly and differences in structure and behavior between that species and P. pseudoechinus are described.     

Extended performance using a high field magnet mass spectrometer

The development of a high field magnet for high mass electron impact, chemical ionization, field desorption and fast atom bombardment mass spectrometric studies is described. Its utility is illustrated with examples from structural studies of vitamin B12 biosynthetic intermediates, oligosaccharides, glycopeptides and the bleomycin antibiotics. The technique has also greatly assisted sequence studies of protein derived peptides.     

Floating lipid bilayers: models for physics and biology

Progress in the determination of structure and fluctuation spectrum of a floating bilayer system, as well as potential applications for biological studies, is reviewed. The system described here was first introduced by Charitat et al. (Eur Phys J B 8:583–593, 1999) and consists of a planar bilayer floating at 2–3?nm away from an adsorbed one on a solid surface in contact with bulk water. This model has been widely used for surface scattering studies using both neutrons and synchrotron radiation and its use in studies of relevance for physics and biology research areas will be described, together with the progress towards the production of complex biomimetic samples for use with scattering techniques.     

Marphysa (Eunicidae,polychaete, Annelida) species of the Sanguinea group from Australia,with comments on pseudo‐cryptic species

The genus Marphysa is widely collected for bait by recreational fishermen in Australia, and yet the number of species remains unresolved. A new species is described together with additional information on a previously described species. This study reveals that while species of Marphysa superficially resemble one another, detailed studies on the distribution of different types of chaetae along the body, together with molecular studies, uncover species which may co‐occur. Correct identification is critical for management of these largely intertidal species. This study also highlights the increasing awareness of the presence of pseudo‐cryptic species in polychaetes.     

A comparative graphical method for the characterization of all types of linear enzyme inhibition

A simple graphical method is described for plotting kinetics for inhibited enzyme-catalyzed reactions. The plots are particularly designed for the characterization of all types of linear enzyme inhibition in comparative studies.     

Identification and quantitative analysis of alkyl isocyanates as their urea derivatives

A gas chromatographic procedure is described for the quantitative analysis of dilute solutions of short-chain alkyl isocyanates. The procedure is based upon measurement of urea derivatives formed from reaction of the isocyanates with amines. Gas chromatographic and thin-layer chromatographic separations of a number of urea derivatives are described. Application of the method is demonstrated in studies of the decomposition of and urea formation from isocyanates in aqueous solutions.     

Humeroradial synostosis

Summary A 1-year-old female child with congenital humeroradial synostosis, who also had microphthalmia and coloboma of the iris and choroidea is described. This is the second case in the literature in which chromosomal studies have revealed no abnormalities. Dermatoglyphic studies are also described.     

Physiological and biochemical studies of fish functional homeostasis

Stages of formation and development of physiological and biochemical studies of fish in Russia have been described. Main directions of studies and biochemical markers used for the assessment of the functional state of fish in natural populations and aquaculture have been described.     

Repetitive recycling of guanosine triphosphate cyclohydrolase I for synthesis of dihydroneopterin triphosphate

A procedure for enzymatic production of dihydroneopterin triphosphate is described that allows GTP cyclohydrolase I to be reused repetitively. The reaction takes place in an ultrafiltration cell, and the product is collected in the filtrate, whereas the enzyme remains in the cell to be reused with additional substrate. This is repeated until the enzyme activity drops below a desirable level. The purity of the dihydroneopterin triphosphate is satisfactory for utilization of this compound for studies on enzymes involved in the synthesis of tetrahydrobiopterin and drosopterin. A procedure for purification of dihydroneopterin triphosphate is described that uses C18-silica and silica cartridges.     

Selector systems in recording physiological and behavioural activity in sedentary aquatic animals

The design and use of selector systems in recording physiological rate functions and behavioural activity in sessile invertebrates, particularly bivalves, is described for use in the laboratory and the field. The experimental limitations of the technique are discussed and the use of switch systems with other types of aktograph is described. Switch systems permit large groups of animals to be used in activity studies, whilst reducing both time spent in data handling and cost per animal of the recording system.     

Purification and properties of 6-phosphogluconate dehydrogenase from sheep liver

A method is described for the isolation of 6-phosphogluconate dehydrogenase from sheep liver. The product appears to be homogeneous in polyacrylamide-gel electrophoresis and in sedimentation-velocity and sedimentation-equilibrium studies in the ultracentrifuge. The molecular weight is estimated as 129000 from equilibrium sedimentation.