共查询到20条相似文献,搜索用时 15 毫秒
1.
2.
3.
4.
5.
6.
Cloned complementary deoxyribonucleic acid probes for untranslated messenger ribonucleic acid components of mouse sarcoma ascites cells 总被引:2,自引:0,他引:2
Mouse sarcoma ascites cells contain several abundant mRNA species that occur to a large extent in an untranslated state. RNA preparations enriched in these species were used as starting material to construct recombinant plasmids. Cloned plasmids bearing sequences homologous to four of the untranslated mRNA species were identified by translation of hybrid-selected material. These plasmids, as well as a recombinant plasmid derived from chick alpha-actin mRNA, were used as probes for the estimation of mRNA levels in polyribosomes and in small ribonucleoprotein (RNP) particles of the ascites cells. Considerable amounts of the mRNA molecules belonging to the untranslated species were present in polyribosomes as well as in mRNPs. The actin mRNA, on the other hand, was present almost exclusively in polyribosomes. The distributions obtained by the hybridization assay resembled those estimated by translation of the same RNA preparations in cell-free systems. This indicates that the mRNA molecules of a given species engaged in translation in the cells and those present as untranslated RNP particles are equally effective in cell-free translation systems. 相似文献
7.
8.
9.
10.
Independent binding sites in mouse 5.8S ribosomal ribonucleic acid for 28S ribosomal ribonucleic acid 总被引:6,自引:0,他引:6
Limited digestion of mouse 5.8S ribosomal RNA (rRNA) with RNase T2 generates 5'- and 3'-terminal "half-molecules". These fragments are capable of independently and specifically binding to 28S rRNA, so there exist at least two contacts in the 5.8S rRNA for the 28S rRNA. The dissociation constants for the 5.8S/28S, 5' 5.8S fragment/28S, and 3' 5.8S fragment/28S complexes are 9 x 10(-8) M, 6 x 10(-8) M, and 13 x 10(-8) M, respectively. Thus, each of the fragment binding sites contributes about equally to the overall binding energy of the 5.8S/28S rRNA complex, and the binding sites act independently, rather than cooperatively. The dissociation constants suggest that the 5.8S rRNA termini from short, irregular helices with 28S rRNA. Thermal denaturation data on complexes containing 28S rRNA and each of the half-molecules of 5.8S rRNA indicate that the 5'-terminal binding site(s) exist(s) in a single conformation while the 3'-terminal site exhibits two conformational alternatives. The functional significance of the different conformational states is presently indeterminate, but the possibility they may represent alternative forms of a conformational switch operative during ribosome function is discussed. 相似文献
11.
12.
13.
14.
15.
16.
Interaction between polyuridylic acid and rabbit globin messenger ribonucleic acid 总被引:4,自引:2,他引:2 下载免费PDF全文
J. A. Hunt 《The Biochemical journal》1973,131(2):327-333
Poly(U) binds to globin mRNA in 0.1m-NaCl. Studies with ribonuclease digestion of this complex suggest that there are polyadenylate-rich sequences in the mRNA containing about 30-40 adenylate residues. The sequences appear to be homogenous and of approximately the same length for both alpha- and beta-globin mRNA. They are most likely located at the 3' terminus of the molecule. 相似文献
17.
Transcription of ribonucleic acid in isolated mouse myeloma nuclei 总被引:29,自引:0,他引:29
18.
Membrane-bound ribonucleic acid in mammalian erythroid cells 总被引:3,自引:0,他引:3
19.
Chromosomal ribonucleic acid of rat ascites cells 总被引:4,自引:0,他引:4
20.
Transfer ribonucleic acid methylases of HeLa cells 总被引:3,自引:0,他引:3