Is there a place for culture in life cycle sustainability assessment?

Purpose

Cultures are increasingly recognised for their inherent value, yet, despite political and societal concern, culture is widely unrecognised in assessment techniques. Life cycle sustainability assessment (LCSA), a technique encompassing environmental, social and economic aspects, is growing in popularity. However, cultural values are rarely considered in LCSA. This paper reviews the meaning of culture; current efforts to include culture in environmental life cycle assessment (LCA), social LCA (S-LCA) and LCSA; and aspects to address when investigating integration of culture in LCA, S-LCA and LCSA.

Methods

A literature review was undertaken on definitions of culture, recognition of culture in policy and decision making, and how culture is incorporated into assessment techniques. The potential for integrating culture in LCSA was evaluated in terms of the potential benefits and challenges.

Results

Culture is often intangible and inaccessible, which may then lead to a lack of recognition in decision-making processes, or if it is recognised, then it is relegated as an afterthought. Explicitly including consideration of culture within LCSA will allow its representation alongside other sustainability aspects. The challenges of representing culture within LCSA include recognising when ‘culture’ should be distinguished from ‘social’; culture’s dynamic nature; the data collection process; and the diversity of cultures between stakeholders and at different scales from community through to nation. The potential benefits of representing culture within LCSA include greater resonance of LCSA results with stakeholders; a more comprehensive decision support tool which appropriately accounts for values; and an assessment technique which may help protect communities and their diversity of cultures.

Conclusions

Representing culture in LCSA is not straightforward and, to some extent, may be addressed through social indicators. However, developing LCSA to explicitly address cultural values has potential benefits. Future research should focus on opportunities for the development of (a) a culturally inclusive LCSA process and (b) additional cultural indicators and/or dimensions of existing LCSA indicators that represent cultural values.     

Is a physiological perspective relevant in a 'genocentric' age?

Currently, the major thrust of plant physiology research is to identify and understand the regulation of genes that might be relevant in plant development and growth. The dominance of a genocentric view of plant behaviour has, unfortunately, resulted in the development of major disconnects in the classical view of plant physiology as a partnership between fundamental and practical research contributing to improved plant production. One disconnect is that much of the genocentric research appears to be organized and executed without regard to the practical needs of enhancing plant performance under applied conditions. Although practical benefits from genocentric research are often claimed, basic assumptions guiding much research and the experimental protocols used are commonly not relevant for real-world plant production. A second disconnect is a failure fully to appreciate the lessons learned in 40 years of classical plant physiology research concerning the role of physiological processes in altering whole plant performance. Regulation of plant systems has proved to be complex and redundant. Alteration of a single physiological process is compensated or dampened so that commonly very little change in plant growth and yield results from modification of a single physiological process. Based on a few successful projects employing classical plant physiology to achieve crop yield increase, key characteristics for research projects that truly seek to increase plant performance in production systems are identified. Basically, the partnership between the fundamental and practical research long espoused for plant physiology needs to be re-established in an intimate and meaningful way.     

Is chromium pharmacologically relevant?

Recent research, combined with reanalysis of previous results, has revealed that chromium can no longer be considered an essential trace element. Clinical studies are ambiguous at best as to whether Cr has a pharmacological effect in humans. Observed effects of Cr on rodent models of insulin resistance and diabetes are best interpreted in terms of a pharmacological role for Cr. Studies on the effects of Cr on rat models of diabetes are reviewed herein and suggest Cr increases insulin sensitivity in peripheral tissues of the rodent models. The lack of effects in human studies may stem from humans receiving a comparably smaller dose than the rodent models. However, given the different responses to Cr in the rodent models, humans could potentially have different responses to Cr.     

Is nitrate an ecologically relevant endocrine disruptor in vertebrates?

The last three decades have brought clear recognition that manypopulations of animals are experiencing severe declines or localand global extinctions. Many examples have become common knowledgeto the general public, such as worldwide declines in amphibianpopulations and extensive loss of coral reefs. The mechanismsunderlying these and other changes are poorly understood. However,a growing literature indicates that a wide array of chemicalcontaminants have the potential to disrupt normal cell-to-cellsignaling mechanisms. A global pollutant of most aquatic systems,nitrate has the potential to be an endocrine disrupting contaminant.This paper reviews studies performed on vertebrates demonstratingthat nitrate and/or nitrite have the potential to alter endocrinefunction. Further, a retrospective study of our work on alligatorsfrom various lakes in Florida suggests that nitrate could contributeto some of the altered endocrine parameters previously reportedin juvenile animals. We propose hypotheses suggesting that nitratecould alter steroidogenesis by 1) conversion to nitrite andnitric oxide in the mitochondria, the site of initial steroidsynthesis, 2) altering Cl^– ion concentrations in the cellby substituting for Cl^– in the membrane transport pumpor 3) binding to the heme region of various P450 enzymes associatedwith steroidogenesis and altering enzymatic action. Future studiesare needed to examine the endocrine disruptive action of thisubiquitous pollutant. A growing literature indicates that allbiologists studying natural systems, whether they choose toor not, must now consider contaminant exposure as a direct influenceon their studies. That is, ubiquitous global contamination hasthe potential to alter the endocrine, nervous and immune systemsof all organisms with resulting changes in gene expression andphenotypes.     

Is floristic quality assessment reliable in human-managed ecosystems?

The Floristic Quality Analysis (FQA) is a method to assess the quality of a flora based on the assignment of scores to plant species and subsequent calculation of indices. This method is widely applied, but inadequate investigation has been devoted to test its potential problems due to human factors. This work is aimed to specifically test how the human factor can affect the calculation of the FQA indices, by addressing three questions: (i) Are the scores given to plant species consistent among different experts?; (ii) Are the floristic quality indices calculated by different experts consistent in ordering individual sites?; and (iii) Does the use of an appropriate statistics change the ordering of individual sites? To answer these questions, a list of species obtained in 136 plots in central Italy was submitted to nine experts, who scored each species. The FQA indices were then calculated from the scores of each of the experts. The results showed that: (i) the scores given to the species by the experts were not consistent and the derived floristic quality indices were statistically different; (ii) the floristic quality indices calculated for each plot were significantly different among experts, but the ranking of these plots based on their floristic quality was rather consistent; and (iii) the use of ordinal statistics, which is more adequate for this type of data, did not change the results. This study demonstrated that the Floristic Quality Analysis does not provide reliable and objective tools to assess the quality of the flora in a human-managed ecosystem. The application of these indices should be preceded with resolution of the methodological problems associated with the use of inappropriate statistics, and by procedures to reduce the degree of subjectivity in assigning the CC scores.     

Is DRM lipid composition relevant in cell-extracellular matrix adhesion structures?

Focal adhesions mediate cell-extracellular matrix adhesion. They are inserted in detergent-resistant membrane microdomains enriched in phosphatidylinositol-4,5-bisphosphate. In spite of the relevance that membrane lipids appear to have on cell adhesion structures, to our knowledge, there are no previous reports on the membrane lipid composition where focal adhesions are located in vivo or on how changes in local membrane composition contribute to focal adhesion maintenance. This may be due to the fact that the explosion of information in the fields of genomics and proteomics has not been matched by a corresponding advancement of knowledge in the field of lipids. The physiological importance of lipids is illustrated by the numerous diseases to which lipid abnormalities contribute. To gain insight into the role of membrane lipid composition in the preservation of epithelial cell adhesion to the substratum, how specific changes in the membrane lipid composition in vivo affect the maintenance of focal adhesions in renal papillae collecting duct cells has been previously studied. It is currently considered that phosphatidylinositol-4,5-bisphosphate plays a crucial role in the maintenance of assembled focal adhesion. However, such pool of polyphosphoinositides has to be part of a domain of a specific lipid composition to serve as a membrane lipid stabilizing the focal adhesion plaque.     

Is there a pilot in a pseudopod?

The concept of pilot pseudopodia is reconsidered 30 years after its inauguration (Gerisch, G., Hülser, D., Malchow, D., Wick, U., 1975. Cell communication by periodic cyclic-AMP pulses. Phil. Trans. R. Soc. Lond. B 272, 181-192). The original hypothesis stated that protruding pseudopodia serve as dynamic sensory organelles that aid a cell in perceiving variations of chemoattractant concentration and, consequently, in navigation during chemotaxis. This influential idea is reevaluated in the light of recent findings about the mechanisms governing chemotactic cell motility, morphology and dynamics of pseudopodia, and about molecular constituents and regulators of pseudopod extension and retraction. It is proposed that stimulation by a chemoattractant modulates speed of pseudopod protrusion and thereby increases cell elongation. Elongation further enhances chemotactic sensitivity of the cell to shallow chemoattractant gradients, reinforces cell polarization, and finally leads to suppression of lateral pseudopodia and continuation of cell migration in the gradient direction.     

Is the global conservation status assessment of a threatened taxon a utopia?

The signatory countries of the Convention on Biological Diversity agreed to significantly reduce the rate of biodiversity loss by 2010. How will we know, however, if we have achieved this goal? Eight groups of hindrances in evaluating the global conservation status of threatened taxon are identified: (1) the extreme heterogeneity and (2) restricted availability of relevant data; (3) the uncertainty in species number and taxonomic division of the given taxon (Linnean shortfall); (4) the fragmentary distribution knowledge (Wallacean shortfall); (5) the incomplete or incorrect red-listing across the whole distribution area; (6) the lack of homogeneous and exhaustive population trend data; (7) the threat knowledge shortfall; (8) the incomplete general biological knowledge on a given taxon. The Linnean and Wallacean shortfalls lay the foundation of all other hindrances. So long as this dramatic shortfall situation does not change, the adequate assessment of the global status for overwhelming majority of extant taxa will remain a utopia.     

Is arginine charged in a membrane?

“Charged” amino acids play countless important roles in protein structure and function. Yet when these side chains come into contact with membranes we do not fully understand their behavior. This is highlighted by a recent model of voltage-gated ion channel activity and translocon-based experiments that suggest small penalties to expose these side chains to lipids, opposing the prevailing view in membrane biophysics. Here we employ a side chain analog as well as a transmembrane helix model to determine the free energy as a function of protonation state and position for a lipid-exposed arginine (Arg) residue across a membrane. We observe high free energy barriers for both the charged and neutral states. Due to the stabilizing influence of membrane deformations for the protonated form, the Arg side chain experiences a pK_a shift of ≤4.5 units and remains mostly protonated. The cost for exposing Arg to lipid hydrocarbon is prohibitively high with implications for many membrane translocating processes and the activation mechanisms of voltage-gated ion channels.     

Is a foetus developing in a sterile environment?

Novel findings in microbiology question the long‐standing paradigm that a healthy pregnancy implies a sterile uterus. It now seems that the placenta is frequently colonized with bacteria, and a placental microbiome has been identified. Recent literature findings are summarized here, and an attempt is made to separate pathological bacterial presence from a naturally occurring microbiome.     

Introducing weights to life cycle sustainability assessment—how do decision-makers weight sustainability dimensions?

Purpose

Decisions based on life cycle sustainability assessment (LCSA) pose a multi-criteria decision issue, as impacts on the three different sustainability dimensions have to be considered which themselves are often measured through several indicators. To support decision-making at companies, a method to interpret multi-criteria assessment and emerging trade-offs would be beneficial. This research aims at enabling decision-making within LCSA by introducing weights to the sustainability dimensions.

Methods

To derive weights, 54 decision-makers of different functions at a German automotive company were asked via limit conjoint analysis how they ranked the economic, environmental, and social performance of a vehicle component. Results were evaluated for the entire sample and by functional clusters. Additionally, sustainability respondents, i.e., respondents that dealt with sustainability in their daily business, were contrasted with non-sustainability respondents. As a last step, the impact of outliers was determined. From this analysis, practical implications for ensuring company-optimal decision-making in regard to product sustainability were derived.

Results and discussion

The results showed a large spread in weighting without clear clustering. On average, all sustainability dimensions were considered almost equally important: the economic dimension tallied at 33.5%, the environmental at 35.2%, and the social at 31.2%. Results were robust as adjusting for outliers changed weights on average by less than 10%. Results by function showed low consistency within clusters hinting that weighting was more of a personal than a functional issue. Sustainability respondents weighted the social before the environmental and economic dimension while non-sustainability respondents put the economic before the other two dimensions. Provided that the results of this research could be generalized, the retrieved weighting set was seen as a good way to introduce weights into an operationalized LCSA framework as it represented the quantification of the already existing decision process. Therefore, the acceptance of this weighting set within the respective company was expected to be increased.

Conclusions

It could be shown that conjoint analysis enabled decision-making within LCSA by introducing weights to solve a multi-criteria decision issue. Furthermore, implications for practitioners could be derived to ensure company-optimal decision-making related to product sustainability. Future research should look at expanding the sample size and geographical scope as well as investigating the weighting of indicators within sustainability dimensions and the drivers that influence personal decision-making in regard to weighting sustainability dimensions.

     

Is there a role for oligosaccharides in seed longevity? An assessment of intracellular glass stability

We examined whether oligosaccharides extend seed longevity by increasing the intracellular glass stability. For that purpose, we used a spin probe technique to measure the molecular mobility and glass transition temperature of the cytoplasm of impatiens (Impatiens walleriana) and bell pepper (Capsicum annuum) seeds that were osmo-primed to change oligosaccharide content and longevity. Using saturation transfer electron paramagnetic resonance spectroscopy, we found that the rotational correlation time of the polar spin probe 3-carboxy-proxyl in the cytoplasm decreased, together with longevity, as a function of increasing seed water content, suggesting that longevity may indeed be regulated by cytoplasmic mobility. Osmo-priming of the seeds resulted in considerable decreases in longevity and oligosaccharide content, while the sucrose content increased. No difference in the glass transition temperature was found between control and primed impatiens seeds at the same temperature and water content. Similarly, there was no difference in the rotational motion of the spin probe in the cytoplasm between control and primed impatiens and bell pepper seeds. We therefore conclude that oligosaccharides in seeds do not affect the stability of the intracellular glassy state, and that the reduced longevity after priming is not the result of increased molecular mobility in the cytoplasm.     

Comparing the incomparable? A review of methodical aspects in the sustainability assessment of wood in vehicles

The International Journal of Life Cycle Assessment - The choice of materials used for a vehicle can contribute to reduce negative environmental and social impacts. Bio-based materials are...     

Is hyper-hardness more chemically relevant than expected?

In this work, the third derivative of the energy with respect to the number of electrons, the so-called hyper-hardness, is investigated to assess whether this quantity has a chemical meaning. To achieve this goal a new working expression for hyper-hardness is developed and analyzed. It transpired from this analysis that hyper-hardness, just like hardness, can measure the reactivity or the stability of electron systems. Interestingly, positive values of hyper-hardness point to quite stable species such as noble gases and molecules. On the other hand, radicals almost always display large negative values of hyper-hardness.