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Ku antigen-DNA conformation determines the activation of DNA-dependent protein kinase and DNA sequence-directed repression of mouse mammary tumor virus transcription. 总被引:1,自引:0,他引:1
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Ward Giffin Wenrong Gong Caroline Schild-Poulter Robert J. G. Hach 《Molecular and cellular biology》1999,19(6):4065-4078
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Zhang X Brann TW Zhou M Yang J Oguariri RM Lidie KB Imamichi H Huang DW Lempicki RA Baseler MW Veenstra TD Young HA Lane HC Imamichi T 《Journal of immunology (Baltimore, Md. : 1950)》2011,186(8):4541-4545
Cytosolic foreign DNA is detected by pattern recognition receptors and mainly induces type I IFN production. We found that transfection of different types of DNA into various untreated cells induces type III IFN (IFN-λ1) rather than type I IFN, indicating the presence of uncharacterized DNA sensor(s). A pull-down assay using cytosolic proteins identified that Ku70 and Ku80 are the DNA-binding proteins. The knockdown studies and the reporter assay revealed that Ku70 is a novel DNA sensor inducing the IFN-lambda1 activation. The functional analysis of IFNL1 promoter revealed that positive-regulatory domain I and IFN-stimulated response element sites are predominantly involved in the DNA-mediated IFNL1 activation. A pull-down assay using nuclear proteins demonstrated that the IFN-λ1 induction is associated with the activation of IFN regulatory factor-1 and -7. Thus, to our knowledge, we show for the first time that Ku70 mediates type III IFN induction by DNA. 相似文献
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酵母PHO2与PHO4蛋白的激活活性的分析及两者的相互作用 总被引:3,自引:3,他引:0
PHO2与PHO4是酵母PHO5基因的两个正调控因子,本文发现,PHO2与酵母转录因子GAL4的DNA结合功能域融合后就能激活报道基因lacZ的表达,其激活力受高低磷影响,表明PHO2蛋白上存在酸性转录激活区。PHO2蛋白上酸性氨基酸丰富的287-326肽段并非PHO2的激活区。在PHO2蛋白上230位Ser处于磷酸化状态2PHO2才有激活作用,表明了这一磷酸化位点可能与PHO2的转录激活能力有关 相似文献
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M West J Mikovits G Princler Y L Liu F W Ruscetti H F Kung 《The Journal of biological chemistry》1992,267(35):24948-24952
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