Scanning electron micrographs of two members of the marine diatom genus Nagumoea, N. africana (the left four photos) and N. serrata (the right two). This genus is characterized as unique by the ladder-like structure of its internal valve as shown in the image. Morphology and molecular phylogeny of this genus are reported by Sugawara et al. in this issue.
Photographs of Kulikovskiyia triundulata gen. et sp. nov. along with holotypic locality. Upper left: Photograph of the holotypic locality (lateritic rocky plateau of Maharashtra, India). Upper middle: Live cell showing chloroplast structure in valve view. Upper right: DIC image of a frustule in valve view. Lower: SEM image of the external valve showing peripheral and apical spines, siliceous slats and tri‐undulate valve shape. See Roy et al. in this issue. Cover picture from: Article link here
The detection of colon cancer by using Poincaré sphere and 2D polarimetric imaging approach. Further details can be found in the article by Deyan Ivanov, Viktor Dremin, Alexander Bykov, Ekaterina Borisova, et al. ( e202000082 ).
Cover Image Cyrtopodium paludicolum. This photo was taken by Dr. João C. F. Cardoso, on the Panga Ecological Station, Uberlândia City, Minas-Gerais state, Brazil.
Infrared spectroscopy enables insight into light‐activated riboflavin‐induced collagen cross‐linking for future intervertebral disc treatment and repair. Further details can be found in the article by Ioannis Vasilikos, Julian Haas, Graciosa Q. Teixeira, Julia Nothelfer, Cornelia Neidlinger‐Wilke, Hans‐Joachim Wilke, Andreas Seitz, Demetrios G. Vavvas, Josef Zentner, Jürgen Beck, Ulrich Hubbe, and Boris Mizaikoff ( e202000110 ).
The cover image is based on the Original Article Regulatory role of human fibrocartilage stem cells in condyle osteochondroma by Qing Yin et al., https://doi.org/10.1111/cpr.13342 .
Cover Image Telipogon peruvianus is a highly restricted Neotropical orchid species, endemic to the southern Peruvian Andes. It is only known from two localities, which are affected by anthropogenic disturbance. The present study investigated whether the restricted distribution of T. peruvianus has led to low genetic diversity and inbreeding, thus threatening its survival. https://doi.org/10.1111/1442-1984.12291
This study proposed a Sparse‐Graph Manifold Learning (SGML) method to balance the sparseness and morphology preserving for bioluminescence tomography reconstruction. It inherits the benefits of non‐convex sparsity constraint and dynamic Laplacian graph model. The results of numerical simulations and in vivo experiments demonstrate that the proposed method yields accurate and robust results in terms of tumor spatial location and morphology recovery. Further details can be found in the article by Hongbo Guo, Ling Gao, Jingjing Yu, et al. ( e201960218 )
Beautiful underwater view of an edible brown alga, Sargassum fusiforme (Fucales, Phaeophyceae) at the rocky shore in Sakurajima, Kagoshima, Japan (Photo by Ryuta Terada). Characteristic responses of the PSII photochemical efficiency on desiccation and salinity gradients in S. fusiforme are reported by Yonemori et al. in this issue.
Optical Coherence Tomography angiography (OCTA) is widely used to image chorioretinal vasculature, with contrast that derives from scattering and motion of red blood cells (RBCs). In the rat eye, a scattering tracer highlights vertical vessels which are not visualized by intrinsic RBC scattering alone. The dependence of microvessel OCTA on angular orientation, explored in this work, represents a potential artifact that should be considered in the clinical interpretation of OCTA. Further details can be found in the article by Jun Zhu, Marcel T. Bernucci, Conrad W. Merkle, and Vivek J. Srinivasan ( e202000090) .
Left panel: Lateral view of a lenticular cell of Valonia utricularis at the beginning (upper) and the end (lower) of a 22-hr observation. Arrowheads with numbers indicate carbon particles along the cell outline. Right panel: (upper) Changes in cell outline and position of the numbered carbon particles in the lenticular cell at 2-hr intervals. (lower) The segmental extension profile of the lenticular cell showing the anisotropic cell surface growth. Numbers represent those used for labelling the carbon particles. See Mine et al. in this issue for details.
Cover Image The pistillate-phase flowers of Artabotrys (Annonaceae): A. blumei (left) and A. brachypetalus (right). Photos by Junhao Chen, taken in Hong Kong and South Africa, respectively (DOI: 10.1111/1442-1984.12273 ).
CRISPR-Cas9 system with PEG-mediated transfection was efficient for genome editing in Ulva prolifera. U. prolifera (left) and male gametes (middle). Wild type (upper right) and the genome-edited strain (lower right) cultured in 2-FA selection medium, and each mutation site on the APT gene. See Ichihara et al. in this issue.
A comparative analysis of intrinsic skin aging between Caucasian and Asian subjects by slide‐free in vivo harmonic generation microscopy is reported. The cellular and nuclear areas of basal cells in Caucasians and Asians are found to increase at the same rate, ideal for scoring age. On the other hand, the viable epidermis thickness, the dermal papilla volume and height in Caucasians are found to decrease faster than those in Asians. Further details can be found in the article by Kuan‐Hung Lin, Yi‐Hua Liao, Ming‐Liang Wei, and Chi‐Kuang Sun ( e201960063 ).
Successful therapy of twin‐to‐twin transfusion syndrome requires accurate imaging to guide laser photocoagulation of the anastomosing placental vessels. Photoacoustic (PA) imaging is an alternative imaging method that provides contrast for hemoglobin, and in this study, it was used to visualize chorionic superficial and subsurface vasculature in human placentas. The strong potential of PA imaging to guide minimally invasive fetal therapies was demonstrated. Further details can be found in the article by Efthymios Maneas, Rosalind Aughwane, Nam Huynh, et al. ( e201900167 ).
iSERS (SERS=surface‐enhanced Raman scattering) microscopy is an emerging Raman‐based staining technique for the selective localization of target proteins on cells and tissues using antibody‐ SERS nanotag conjugates. In this contribution we demonstrate the feasibility of iSERS for imaging of programmed cell death‐ligand 1 (PD‐L1), an important predictive biomarker, on single SkBr‐3 breast cancer cells. Further details can be found in the article by Elzbieta Stepula, Matthias König, Xin‐Ping Wang, et al. ( e201960034 ).
