CRISPR-Cas9 system with PEG-mediated transfection was efficient for genome editing in Ulva prolifera. U. prolifera (left) and male gametes (middle). Wild type (upper right) and the genome-edited strain (lower right) cultured in 2-FA selection medium, and each mutation site on the APT gene. See Ichihara et al. in this issue.
Left panel: Lateral view of a lenticular cell of Valonia utricularis at the beginning (upper) and the end (lower) of a 22-hr observation. Arrowheads with numbers indicate carbon particles along the cell outline. Right panel: (upper) Changes in cell outline and position of the numbered carbon particles in the lenticular cell at 2-hr intervals. (lower) The segmental extension profile of the lenticular cell showing the anisotropic cell surface growth. Numbers represent those used for labelling the carbon particles. See Mine et al. in this issue for details.
A tidal pool dinoflagellate, Chiharadinium hexapraecingulum (T. Horiguchi & Chihara) Dawut & T. Horiguchi gen. & comb. nov. Upper row: Light micrographs of lateral view, ventral view, epifluorescence micrograph of motile cells and scanning electron micrograph of apical view of a cell. Lower row: Scanning electron micrographs of ventral view, left lateral view and antapical view of motile cells. Establishment of a new genus Chiharadinium is reported by Dawut et al. in this issue.
A Orobanche boninsimae and its visitors, Japanese white-eyes (Zosterops japonicus). Photo by Akihiro Nishimura at Chichijima Island, the Bonin Islands, Japan. Nishimura & Takayama (2023) “First record of potential bird pollination in the holoparasitic genus Orobanche L.” Plant Species Biology, 6–17 https://doi.org/10.1111/1442-1984.12389
Cover Image An infl orescence of Clerodendrum izuinsulare (left) and C. trichotomum (right). Photos by Natsuki Aihara in Niijima Island (left) and by Takashi Miyake in Izu Peninsula (right), Japan. Miyake et al., doi.org/10.1111/1442-1984.12287
Scanning electron micrographs of two members of the marine diatom genus Nagumoea, N. africana (the left four photos) and N. serrata (the right two). This genus is characterized as unique by the ladder-like structure of its internal valve as shown in the image. Morphology and molecular phylogeny of this genus are reported by Sugawara et al. in this issue.
Peptoids belong to a class of sequence-controlled polymers containing N-alkylglycine units. Ren et al. use tandem mass spectrometry to characterize the fragmentation patterns of some singly and doubly protonated peptoids with one basic residue at different positions. Upon collision-induced dissociation, a charged peptoid dissociates into two sets of charged fragments: B-ions, containing the N-terminal residue (structure on the left); and Y-ions, containing the C-terminal residue (structure on the right). The Y-ions are of higher abundance than the B-ions, revealing that the C-terminal fragments have a higher affinity toward the charge carrier, the proton. (DOI: 10.1002/bip.23358 )
Cover Photograph: Upper left: Newt (P. waltl) juveniles and Xenopus froglets were subjected to agarose-embedded heat-shock (AeHS) method. GFP expression was induced specifically in the regenerating limbs (see Matsubara et al. 86–93). Upper right: Schematic diagram of the intermittent hypoxia exposure model. Pimonidazole-positive cells were detected in the pulmonary tissues of Xenopus laevis during repeated dives, indicating tissue hypoxia (see Fujiyama, 94–99). Lower left: The intercalary element of Zhangixalus schlegelii (arrowheads). Such skeletal elements are seen between the terminal and subterminal phalanges of all the digits of arboreal frogs (see Nakanishi, 100–108). Lower right: Tetraspanin9 expressions in Xenopus laevis (above) and X. tropicalis embryo (below) hybridized in the same tube. (see Kuriyama and Tanaka, 109–119). Bottom right corner: A 48 hpf early four-armed pluteus taken through a confocal microscope. Catecholamine-positive cells are colored in yellow (see Kalachev and Tankovich, 120–131).
Lake Inle, a pristine lake in Myanmar, is known to harbor a number of endemic aquatic species and is a home to an enigmatic cyanobacterium Oscillatoria kawamurae, which was first reported in Lake Biwa (Japan). Macroscopic trichome (upper left), microscopic image (upper middle). Photos by Phong San (Lake Inle) and Nanda Kyaw Thu (O. kawamurae). See Thu et al. in this issue. Cover picture from: Article link here
Cover Image Tulipa pumila. This photo was taken by Sandro Pratesi at Doccino at the municipality of Riparbella, province of Pisa, Tuscany, Italy (DOI: 10.1111/1442-1984.12267 ).
The detection of colon cancer by using Poincaré sphere and 2D polarimetric imaging approach. Further details can be found in the article by Deyan Ivanov, Viktor Dremin, Alexander Bykov, Ekaterina Borisova, et al. ( e202000082 ).
Beautiful underwater view of an edible brown alga, Sargassum fusiforme (Fucales, Phaeophyceae) at the rocky shore in Sakurajima, Kagoshima, Japan (Photo by Ryuta Terada). Characteristic responses of the PSII photochemical efficiency on desiccation and salinity gradients in S. fusiforme are reported by Yonemori et al. in this issue.
A dual‐raster‐scanned photoacoustic microscope (DRS‐PAM) was reported, which integrates a two‐dimensional motorized translation stage for large field‐of‐view imaging and a two‐axis fast galvanometer scanner for real‐time imaging. The DRS‐PAM provides a flexible transition from wide‐field monitoring the vasculature of organs to real‐time imaging of local dynamics. Further details can be found in the article by Fei Yang, Zhiyang Wang, Wuyu Zhang, et al. ( e202000022 ).
An optical fan was demonstrated to screen leukemia cells from the blood sample at the single‐cell level in a noninvasive and noncontact manner. Further details can be found in the article by Xiaoshuai Liu, Yuchao Li, Xiaohao Xu, Yao Zhang, Baojun Li ( e201900155 ).
A type of compact and cost‐effective light‐sheet imaging device, termed sub‐voxel‐resolving light‐sheet add‐on module (SLAM), is developed to cooperate with conventional 2D epifluorescence microscope, allowing high‐contrast, resolution‐improved 3D imaging of various biological samples at high throughput. Further details can be found in the article by Fang Zhao, Yicong Yang, Yi Li, et al. ( e201960243 ).
Immunofluorescence image of Plasmodium berghei sporozoites (red) in HeLa cells 1.5 hours post infection. The parasitophorous vacuole membrane (PVM) that surrounds the parasites was stained with antibodies against the PVM‐resident protein UIS4 (grey). DNA was stained with DAPI (blue). Imaging was performed on a Leica SP8 confocal microscope. For further details, readers are referred to the article by Bindschedler et al. on p. e13271 of this issue.
Cover Image The pistillate-phase flowers of Artabotrys (Annonaceae): A. blumei (left) and A. brachypetalus (right). Photos by Junhao Chen, taken in Hong Kong and South Africa, respectively (DOI: 10.1111/1442-1984.12273 ).
Infrared spectroscopy enables insight into light‐activated riboflavin‐induced collagen cross‐linking for future intervertebral disc treatment and repair. Further details can be found in the article by Ioannis Vasilikos, Julian Haas, Graciosa Q. Teixeira, Julia Nothelfer, Cornelia Neidlinger‐Wilke, Hans‐Joachim Wilke, Andreas Seitz, Demetrios G. Vavvas, Josef Zentner, Jürgen Beck, Ulrich Hubbe, and Boris Mizaikoff ( e202000110 ).
