共查询到20条相似文献,搜索用时 15 毫秒
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Background
The metastatic ability of tumor cells is determined by level of expression of specific genes that may be identified with the aid of cDNA microarray containing thousands of genes and can be used to establish the expression profile of disease related genes in complex biological system. 相似文献2.
David Honys Sung-Aeong Oh David Reňák Maarten Donders Blanka Šolcová James Andrew Johnson Rita Boudová David Twell 《BMC plant biology》2006,6(1):31-9
Background
The effective functional analysis of male gametophyte development requires new tools enabling the spatially and temporally controlled expression of both marker genes and modified genes of interest. In particular, promoters driving expression at earlier developmental stages including microspores are required. 相似文献3.
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Background
Expression array data are used to predict biological functions of uncharacterized genes by comparing their expression profiles to those of characterized genes. While biologically plausible, this is both statistically and computationally challenging. Typical approaches are computationally expensive and ignore correlations among expression profiles and functional categories. 相似文献5.
Genome mapping and expression analyses of human intronic noncoding RNAs reveal tissue-specific patterns and enrichment in genes related to regulation of transcription 下载免费PDF全文
Nakaya HI Amaral PP Louro R Lopes A Fachel AA Moreira YB El-Jundi TA da Silva AM Reis EM Verjovski-Almeida S 《Genome biology》2007,8(3):R43
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Background
A key step in the analysis of microarray expression profiling data is the identification of genes that display statistically significant changes in expression signals between two biological conditions. 相似文献7.
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Birte Hellwig Jan G Hengstler Marcus Schmidt Mathias C Gehrmann Wiebke Schormann Jörg Rahnenführer 《BMC bioinformatics》2010,11(1):276
Background
A major goal of the analysis of high-dimensional RNA expression data from tumor tissue is to identify prognostic signatures for discriminating patient subgroups. For this purpose genome-wide identification of bimodally expressed genes from gene array data is relevant because distinguishability of high and low expression groups is easier compared to genes with unimodal expression distributions. 相似文献9.
Selection of reference genes for gene expression studies in human neutrophils by real-time PCR 总被引:1,自引:0,他引:1
Background
Reference genes, which are often referred to housekeeping genes, are frequently used to normalize mRNA levels between different samples. However the expression level of these genes may vary among tissues or cells, and may change under certain circumstances. Thus the selection of reference gene(s) is critical for gene expression studies. For this purpose, 10 commonly used housekeeping genes were investigated in isolated human neutrophils. 相似文献10.
Federico E Turkheimer Federico Roncaroli Benoit Hennuy Christian Herens Minh Nguyen Didier Martin Annick Evrard Vincent Bours Jacques Boniver Manuel Deprez 《BMC bioinformatics》2006,7(1):526
Background
Expression microarrays represent a powerful technique for the simultaneous investigation of thousands of genes. The evidence that genes are not randomly distributed in the genome and that their coordinated expression depends on their position on chromosomes has highlighted the need for mathematical approaches to exploit this dependency for the analysis of expression data-sets. 相似文献11.
Background
Recent circadian clock studies using gene expression microarray in two different tissues of mouse have revealed not all circadian-related genes are synchronized in phase or peak expression times across tissues in vivo. Instead, some circadian-related genes may be delayed by 4–8 hrs in peak expression in one tissue relative to the other. These interesting biological observations prompt a statistical question regarding how to distinguish the synchronized genes from genes that are systematically lagged in phase/peak expression time across two tissues. 相似文献12.
Background
One of the important goals of microarray research is the identification of genes whose expression is considerably higher or lower in some tissues than in others. We would like to have ways of identifying such tissue-specific genes. 相似文献13.
Ramdas L Cogdell DE Jia JY Taylor EE Dunmire VR Hu L Hamilton SR Zhang W 《BMC genomics》2004,5(1):35-9
Background
DNA microarrays using long oligonucleotide probes are widely used to evaluate gene expression in biological samples. These oligonucleotides are pre-synthesized and sequence-optimized to represent specific genes with minimal cross-hybridization to homologous genes. Probe length and concentration are critical factors for signal sensitivity, particularly when genes with various expression levels are being tested. We evaluated the effects of oligonucleotide probe length and concentration on signal intensity measurements of the expression levels of genes in a target sample. 相似文献14.
Background
It is widely accepted that orthologous genes between species are conserved at the sequence level and perform similar functions in different organisms. However, the level of conservation of gene expression patterns of the orthologous genes in different species has been unclear. To address the issue, we compared gene expression of orthologous genes based on 2,557 human and 1,267 mouse samples with high quality gene expression data, selected from experiments stored in the public microarray repository ArrayExpress. 相似文献15.
Background
Normalization of gene expression microarrays carrying thousands of genes is based on assumptions that do not hold for diagnostic microarrays carrying only few genes. Thus, applying standard microarray normalization strategies to diagnostic microarrays causes new normalization problems. 相似文献16.
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Background
The analysis of the promoter sequence of genes with similar expression patterns is a basic tool to annotate common regulatory elements. Multiple sequence alignments are on the basis of most comparative approaches. The characterization of regulatory regions from co-expressed genes at the sequence level, however, does not yield satisfactory results in many occasions as promoter regions of genes sharing similar expression programs often do not show nucleotide sequence conservation. 相似文献19.
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Evert-Jan Blom Sacha AFT van Hijum Klaas J Hofstede Remko Silvis Jos BTM Roerdink Oscar P Kuipers 《BMC bioinformatics》2008,9(1):535