共查询到20条相似文献,搜索用时 484 毫秒
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A major plasma protein, referred to as SP 1, exhibits sex- and stage-specific expression during the larval development of the silkworm, Bombyx mori. We have isolated a plasmid clone bearing a part of mRNA sequence coding for SP 1 and quantitated the amount of SP 1 mRNA by means of RNA blot hybridization using the cDNA probe. The developmental change in the amount of SP 1 mRNA in the fat body closely reflected that of the hemolymph concentration of SP 1, indicating that the biosynthesis of SP 1 is regulated in a sex- and developmental stage-specific fashion at the level of mRNA. 相似文献
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Biosynthesis of major plasma proteins in the primary culture of fat body cells from the silkworm, Bombyx mori 总被引:2,自引:0,他引:2
Atsuhiro Kishimoto H. Nakato Susumu Izumi Shiro Tomino 《Cell and tissue research》1999,297(2):329-335
Plasma proteins termed "SP1" and "30K proteins" are synthesized by the fat body cells of the silkworm, Bombyx mori, in a sex- and stage-specific manner during larval development. We successfully established a primary culture of the fat body cells in order to investigate the regulatory mechanisms of plasma protein gene expression. The primary cultures of fat body cells contained at least two cell types: small oval cells, and large spherical cells. The cells adhered to and migrated on the cultured dish after plating. By the 7th day of cultivation, the cells clustered to form fat body-like structures, which were maintained for at least 3 months. Plasma proteins were actively synthesized in the primary cultures of the fat body cells isolated from the final instar larvae only when the cells tightly adhered to and clustered on the cultured dish. Immunocytochemical analysis revealed that only 10-15% of the clustered cells synthesized plasma proteins in our culture system, indicating that the primary culture comprises heterogeneous cells that are morphologically and functionally distinct. The patterns of SP1 syntheses in primary cultures faithfully reproduced their sex-dependency in vivo. 相似文献
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V Joulin M C Garel P Le Boulch C Valentin R Rosa J Rosa M Cohen-Solal 《The Journal of biological chemistry》1988,263(30):15785-15790
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A gene encoding rat cholecystokinin. Isolation, nucleotide sequence, and promoter activity 总被引:14,自引:0,他引:14
R J Deschenes R S Haun C L Funckes J E Dixon 《The Journal of biological chemistry》1985,260(2):1280-1286
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Cloning and analysis of the rat gamma-glutamyltransferase gene 总被引:4,自引:0,他引:4
S Rajagopalan J H Park P D Patel R M Lebovitz M W Lieberman 《The Journal of biological chemistry》1990,265(20):11721-11725
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Structure of the rat L-type pyruvate kinase gene 总被引:10,自引:0,他引:10
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Isolation and characterization of the mouse ornithine decarboxylase gene 总被引:15,自引:0,他引:15