cDNA library construction from meristematic tissue of finger millet panicle

A protocol to obtain full-length cDNA using a SuperScript® Full-Length cDNA Library Construction Kit II (Invitrogen, United States) was developed, and a high quality cDNA library of meristem tissue of finger millet panicle (Eleusine coracana (L.) Gaertn) was constructed. The titer of the constructed cDNA library was 3.01 × 10⁵ CFU/mL, the average length of the insert was approximately 1070 base pairs, and the average efficiency of insertion of cDNA fragments was 99.5%. The sequencing of randomly selected clones created cDNA library was carried out. The cDNA sequences of clones were identified by BLAST search. The cDNA library analysis and selective sequencing indicate good functionality and full size of cDNA inserts of the clones. The constructed cDNA library from meristematic tissue of finger millet panicle is a good and reliable source for isolation and identification of key genes of metabolism and development of meristem as well for creation of new genetic markers for genetic research and molecular selection.     

Immune response in human subjects at high altitude

Levels of immunoglobulins were determined in persons exposed to high altitude. The individuals studied included high altitude natives, sea level residents at high altitude for 2 years, and recent arrivals at high altitude. Increased IgG and IgA levels were found in high altitude natives and sea level residents at high altitude for 2 years when compared with sea level residents. In recent arrivals marked increase of IgG and IgG levels and slight rise in IgM was seen. Recent arrivals who suffered from high altitude pulmonary oedema showed marked elevation of IgG, IgM, and IgA. Immunoglobulin responses to both primary and secondary TAB inoculation were of a higher magnitude and more sustained at high altitude than at sea level.