Cancer-associated fibroblasts-derived exosomal miR-3656 promotes the development and progression of esophageal squamous cell carcinoma via the ACAP2/PI3K-AKT signaling pathway

Esophageal squamous cell carcinoma (ESCC) is one of the most common gastrointestinal tumors, accounting for almost half a million deaths per year. Cancer-associated fibroblasts (CAFs) are the major constituent of the tumor microenvironment (TME) and dramatically impact ESCC progression. Recent evidence suggests that exosomes derived from CAFs are able to transmit regulating signals and promote ESCC development. In this study, we compared different the component ratios of miRNAs in exosomes secreted by CAFs in tumors and with those from normal fibroblasts (NFs) in precancerous tissues. The mRNA level of hsa-miR-3656 was significantly upregulated in the former exosomes. Subsequently, by comparing tumor cell development in vitro and in vivo, we found that the proliferation, migration and invasion capabilities of ESCC cells were significantly improved when miR-3656 was present. Further target gene analysis confirmed ACAP2 was a target gene regulated by miR-3656 and exhibited a negative regulatory effect on tumor proliferation. Additionally, the downregulation of ACAP2 triggered by exosomal-derived miR-3656 further promotes the activation of the PI3K/AKT and β-catenin signaling pathways and ultimately improves the growth of ESCC cells both in vitro and in xenograft models. These results may represent a potential therapeutic target for ESCC and provide a new basis for clinical treatment plans.     

基于MFA和DEA的区域经济环境效率评价-以辽宁省为例

对经济与环境效率的科学评价是实现区域可持续发展的前提。运用物质流分析将辽宁省经济系统中数据进行物质化处理,再利用改进的数据包络分析模型对环境和经济效率进行综合评价。结果表明:辽宁省物资消费不主要依赖于进口,向其它地区物质输出量大;环境效率评价的综合效率主要受规模因素影响而显著低于纯技术效率,而整体经济的综合效率却主要受纯技术效率影响而下降。第二产业比重依然偏大的产业结构特征是导致上述结果的主因。进一步改造提升传统产业,发展战略性新兴产业,提高第三产业发展水平,扩大环保规模,促进居民生活质量水平全面提升,将是辽宁省以及与之相似的资源依赖型区域可持续发展的方向。     

HIV/AIDS in Asia： The Shape of Epidemics and Their Molecular Epidemiology

The Asia-Pacific region is a home to 60% of the population in the world and to approximately one quarter of people with HIV/AIDS. Close to a million of people has been infected and a half million people died of AIDS annually in Asia,becoming the second largest epicenter of global AIDS epidemic. Molecular epidemiology has been useful tool to track a course of HIV spread. In-depth knowledge from the studies on molecular epidemiology elucidates the dynamics of HIV spread and the interrelationship of epidemics in the different regions in Asia.     

Opportunities for avoidance of land‐use change through substitution of soya bean meal and cereals in European livestock diets with bioethanol coproducts

An analysis is presented which quantifies the potential for distillers dried grains with solubles (DDGS, a coproduct of wheat bioethanol production) to replace soya bean meal (SBM) and cereals in livestock rations. A major proportion of the SBM imported into Europe as a protein‐rich feedstuff for livestock comes from South America, where land‐use change (LUC) is associated with high carbon emissions. Production of DDGS can therefore reduce LUC in South America by substitution of SBM in animal feed. The analysis indicates that a single bioethanol distillery processing 1 million tonnes of wheat, and producing ca. 330 000 tonnes of DDGS per annum, would substitute at least 136 493 tonnes of whole soya beans grown on 47 725 ha of land, and save greenhouse gas emissions equivalent to 0.63 million tonnes CO₂ per annum. By growing sugar beet and wheat in an average ratio of 0.06 : 0.94 on 1 ha of land in Europe, the net area of agricultural land required to produce feed ingredients equivalent to 6.08 t of sugar beet pulp (SBP) and 1.72 t of DDGS associated with 2363 L of bioethanol, is reduced to 0.40 ha. This accounts for 0.42 ha of soya that is not required when DDGS displaces SBM, and 0.18 ha of wheat that is not required when DDGS and SBP displace wheat in livestock rations.     

HIV/AIDS in Asia: The shape of epidemics and their molecular epidemiology

The Asia-Pacific region is a home to 60% of the population in the world and to approximately one quarter of people with HIV/AIDS. Close to a million of people has been infected and a half million people died of AIDS annually in Asia, becoming the second largest epicenter of global AIDS epidemic. Molecular epidemiology has been useful tool to track a course of HIV spread. In-depth knowledge from the studies on molecular epidemiology elucidates the dynamics of HIV spread and the interrelationship of epidemics in the different regions in Asia. Foundation items: Grant support from Ministry of Health, Labour and Welfare and Ministry of Education, Science and Technology in Japan; Japanese Foundation for AIDS Prevention.     

Producer Responsibility Organizations Development and Operations

Extended producer responsibility (EPR) regulations are now in effect in 27 European Union member states and are applicable to up to 100 million tonnes of waste packaging, batteries, automobiles, and electrical and electronic products annually. This article investigates the implementation of EPR through a case study of European Recycling Platform (ERP) UK Ltd., the UK arm of one of the largest producer responsibility organizations (PROs) in Europe, recycling more than 1.5 million tonnes of waste electrical and electronic equipment to date. Previous research is extremely limited on the detailed operations of PROs. This case is presented as an example illustrating typical operational challenges PROs face in implementing EPR, such as how PROs gain an understanding of the waste management infrastructure and legislation in each country, collect sufficient volumes of waste using cost‐effective arrangements, and maintain uninterrupted collection, treatment, and recycling services. The case study provides new insights and context on the practical implementation of EPR regulations relevant for both policy makers and researchers.     

木薯的组织培养和基因转化

木薯是重要的热带作物之一, 其块根富含淀粉, 是热带、亚热带地区近５ 亿人粮食的主要来源。简单介绍近年来运用生物技术改良木薯的研究进展。     

Antitumor effects of human recombinant macrophage colony-stimulating factor against rat brain tumors

The tumoricidal effects of M-CSF were examined using two subcutaneously-transplanted rat brain tumor cell lines, 9L and T9 gliomas. In rats treated with high-dose M-CSF (16 million U/kg administered for 4 days a week for 3 weeks), 9L glioma growth was inhibited by 81.9% following subcutaneous (s.c.) injection and by 70.5% after intraperitoneal (i.p.) injection and T9 glioma growth was inhibited by 69.2% after i.p. injection. After short-term treatment with high-dose M-CSF (32 million U/kg administered s.c. for 6 consecutive days, 9L glioma growth was inhibited by 82.1%. All these inhibitory effects differed significantly compared with the respective untreated control groups. However, treatment with low-dose M-CSF (1.6 million U/kg administered s.c. for 4 days a week for 3 weeks) showed no significant effects against 9L and T9 glioma growth compared with the untreated controls. No significant effects of M-CSF against cell proliferation, measured as PCNA expression, were observed in any group. Significant hematopoietic effects on the leukocyte counts were observed only in the groups treated with high dose M-CSF. These results suggest that M-CSF at a high dose which produces hematopoietic effects on peripheral leukocytes inhibits the growth of gliomas. This inhibitory effect may have been due to a tumoricidal mechanism of M-CSF that depended on the production or release of some hematopoietic soluble factors, but was independent of PCNA expression by the tumors.Abbreviations BBB blood-brain barrier - G-CSF granulocyte colony-stimulating factor - GM-CSF granulocyte-macrophage colony-stimulating factor - hM-CSF human macrophage colony-stimulating factor - IFN interferon - IL-1 interleukin-1 - IL-6 interleukin-6 - M-CSF macrophage colony-stimulating factor - PCNA proliferating cell nuclear antigen - rhM-CSF recombinant human macrophage colony-stimulating factor - TNF tumor necrosis factor     

Quantity,Components, and Value of Waste Materials Landfilled in the United States

The current system of production and consumption needs end‐of‐life disposal to function, but the linkage between upstream production‐consumption with the downstream landfill as terminus is, at best, a tenuous, one‐way relationship, suggesting a partial system failure. A starting point to fix this link is to confront, systematically, the messy “black box” that is mixed waste landfilling, interrogate its contents locally, and determine a baseline that can be used to scale up results. Here, we develop a detailed model characterizing landfilled municipal solid waste (MSW) in the United States across the dimensions of material quantity, quality, location, and time. The model triangulates measurements spanning 1,161 landfills (representing up to 95% of landfilled MSW) and 15,169 solid waste samples collected and analyzed at 222 sites across the United States. We confirm that landfilled quantities of paper (63 million megagrams [Mg]), food waste (35 million Mg), plastic (32 million Mg, textiles (10 million Mg), and electronic waste (3.5 million Mg) are far larger than computed by previous top‐down U.S. government estimates. We estimate the cost of MSW landfill disposal in 2015 (10.7 billion U.S. dollars [USD]) and gross lost commodity value of recyclable material (1.4 billion USD). Further, we estimate landfill methane emissions to be up to 14% greater (mass basis) than the 2015 U.S. inventory. By principally relying on measurements of waste quantity and type that are recorded annually, the model can inform more effective, targeted interventions to divert waste materials from landfill disposal, improve local, regional, and national emission estimates, enhance dissipative loss estimates in material flow analyses, and illuminate the dynamics linking material, energy, and economic dimensions to production, consumption, and disposal cycles.     

Glaucoma database

Glaucoma, a complex heterogenous disease, is the leading cause for optic nerve-related blindness worldwide. Primary open angle glaucoma (POAG) is the most common subset and by the year 2020 it is estimated that approximately 60 million people will be affected. MYOC, OPTN, CYP1B1 and WDR36 are the important candidate genes. Nearly 4% of the glaucoma patients have mutation in any one of these genes. Mutation in any of these genes causes disease either directly or indirectly and the severity of the disease varies according to position of the genes. We have compiled all the related mutations and SNPs in the above genes and developed a database, to help access statistical and clinical information of particular mutation. This database is available online at http:bicmku.in:8081/glaucoma The database, constructed using SQL, contains data pertaining to the SNPs and mutation information involved in the above genes and relevant study data. AVAILABILITY: The database is available for free at http:bicmku.in:8081/glaucoma.     

Assessing the Greenhouse Gas Savings Potential of Extended Producer Responsibility for Mattresses and Boxsprings in the United States

Extended producer responsibility (EPR) legislation in the United States, which currently only exists on the state level, now includes three mattress EPR acts, which intend to shift the financial and operational burden of mattress end‐of‐life (EOL) management away from local and state government. It is important to keep in mind, however, that the original objective behind EPR is to reduce the environmental life cycle impacts of products. This article therefore quantifies the greenhouse gas (GHG) savings potential of mattress and boxspring recycling and reuse in the United States and also discusses labor implications and mattress design issues. We find that all three acts are unlikely to generate redesign incentives, but are expected to dramatically increase mattress collection and recycling. The collection and recycling of all 35 million EOL mattress and boxspring units estimated to reach the end of their lives in the United States every year would generate in the order of 10,000 jobs and GHG savings between 1 and 1.5 million metric tonnes.     

重庆市都市圈水资源承载力分析与预测

重庆市都市圈目前处于快速城市化的历史阶段,水资源是决定该地区社会经济发展规模及能否得以实现可持续发展的关键因素之一.运用区域水资源的供需平衡模型,预测了重庆市都市圈2020年的水资源承载力.研究表明,至2020年,该区域水资源承载力在880万～1400万人之间,短时期内不会成为社会经济发展的主要限制因素,但在全球气候和区域生态环境变化的影响下,水资源可利用总量的变化对于承载力有较大的影响;过境水可利用量是影响重庆市都市圈水资源承载力的最为重要的因素,因此上游区域生态环境的变化对该区域水资源承载力具有极为重要的影响,并为重庆市都市圈社会经济可持续发展的水资源保障提出了具体建议.     

HIV and HCV： from Co-infection to Epidemiology, Transmission, Pathogenesis, and Treatment

Human immunodeficiency virus (HIV) is the infectious agent causing acquired immu-nodeficiency syndrome (AIDS),a deadliest scourge of human society. Hepatitis C virus (HCV) is a major causative agent of chronic liver disease and infects an estimated 170 million people worldwide,resulting in a serious public health burden. Due to shared routes of transmission,co-infection with HIV and HCV has become common among individuals who had high risks of blood exposures. Among hemophiliacs the co-infection rate accounts for 85%; while among injection drug users (IDU) the rate can be as high as 90%. HIV can accelerate the progression of HCV-related liver disease,particularly when immunodeficiency has developed. Although the effect of HCV on HIV infection is controversial,most studies showed an increase in mortality due to liver disease. HCV may act as a direct cofactor to fasten the progression of AIDS and decrease the tolerance of highly active antiretroviral therapy (HARRT). Conversely,HAART-related hepatotoxicity may enhance the progression of liver fibrosis. Due to above complications,co-infection with HCV and HIV-1 has imposed a critical challenge in the management of these patients. In this review,we focus on the epidemiology and transmission of HIV and HCV,the impact of the two viruses on each other,and their treatment.     

HIV and HCV: from Co-infection to epidemiology, transmission, pathogenesis, and treatment

Human immunodeficiency virus (HIV) is the infectious agent causing acquired immunodeficiency syndrome (AIDS), a deadliest scourge of human society. Hepatitis C virus (HCV) is a major causative agent of chronic liver disease and infects an estimated 170 million people worldwide, resulting in a serious public health burden. Due to shared routes of transmission, co-infection with HIV and HCV has become common among individuals who had high risks of blood exposures. Among hemophiliacs the co-infection rate accounts for 85%; while among injection drug users (IDU) the rate can be as high as 90%. HIV can accelerate the progression of HCV-related liver disease, particularly when immunodeficiency has developed. Although the effect of HCV on HIV infection is controversial, most studies showed an increase in mortality due to liver disease. HCV may act as a direct cofactor to fasten the progression of AIDS and decrease the tolerance of highly active antiretroviral therapy (HARRT). Conversely, HAART-related hepatotoxicity may enhance the progression of liver fibrosis. Due to above complications, co-infection with HCV and HIV-1 has imposed a critical challenge in the management of these patients. In this review, we focus on the epidemiology and transmission of HIV and HCV, the impact of the two viruses on each other, and their treatment.      

Determination of the extraction kinetics for the quantification of polyhydroxyalkanoate monomers in mixed microbial systems

For the first time, a systematic approach was conducted to determine the key factors influencing the kinetics of hydroxyalkanote (HA) extraction in biological systems. Six mixed microbial systems where polyhydroxyalkanoate (PHA) is produced were evaluated. Experiments were carried out for full-scale and lab-scale activated sludge systems using different configurations (containing floccular or granular sludge), as well as specific PHA accumulating cultures that contain high or low intracellular PHA fractions. The overall reaction was limited by the kinetics of the PHA hydrolysis in floccular cultures, whereas in granular cultures, it was limited by the cell lysis step. The monomeric composition of the polymer also had an impact on the HA extraction rate: higher acid concentration and a longer digestion time should be employed when cells accumulate monomers with more substituents, such as hydroxy-2-methylbutyrate (H2MB) and hydroxy-2-methylvalerate (H2MV). This study optimised the method for HA extraction, which impacts the assessment of the quantity and quality of PHA biopolymers.     

Mouse models for the genetic study of tuberculosis susceptibility.

Tuberculosis, mainly caused by the pathogenic bacterium Mycobacterium tuberculosis, remains an inestimable public health problem, despite the established use of the Bacillus Calmette-Guerin (BCG) vaccine, multidrug therapy and the existence of global tuberculosis control programmes. Statistics show that nearly 2 billion people (approximately one-third of the world's population) are infected with M. tuberculosis. For unknown reasons, only about 10 per cent of those infected by M. tuberculosis will develop tuberculosis, resulting in 9 million new cases yearly and 2 million deaths. A better understanding of the host--mycobacterial--environmental interplay is central to developing better antituberculosis vaccines and treatments. This review will discuss how a clearer idea of this interplay is emerging with new genomic strategies in mouse models.     

Oxygen Dependence of Elongation Growth and Oxygen Uptake in Maize Coleoptiles

Auxin-mediated elongation growth of maize coleoptile segments is inhibited by reducing the O₂ concentration in the incubation medium to GT 100 μmol . 1^?1. The half-maximal elongation rate is reached at 40 μmol . 1^?1 O₂, i.e. about two orders of magnitude higher than with mitochondrial respiration. O₂ uptake of the segments measured under similar conditions with an O₂ electrode shows a very similar dependence on O₂ concentration. Auxin increases O₂ uptake by 5–10% when it induces growth. About 40% of the O₂ uptake is insensitive to inhibition by KCN. Auxin has no effect on O₂ uptake in the presence of KCN. The possibility that auxin-mediated elongation growth depends on a KCN-sensitive oxidative process, other than cytochrome c oxidase-catalyzed respiration, is discussed.     

白（既鱼）豚保护研究的进展

白鲃豚是一种仅存于中国的淡水豚,迄今已有约2500万年的历史.20年来,白鲃豚的种群数量急剧减少.其数量从20世纪80年代初的约400头,锐减到90年代末不足100头.白鲃豚的生存环境也急剧恶化,目前仅在长江中下游的干流中有分布.导致白鲃豚濒危的主要原因源于日益增多的人类活动,包括水利工程、渔业活动、水体污染等.白鲃豚的保护主要从3个方面进行:①建立白鲃豚自然保护区,保护其自然种群和栖息地环境;②建立半自然保护区,实行迁地保护;③建立细胞库、基因库等,保护其种质资源.迁地保护为目前最为紧迫的保护措施.     

甘氨鹅脱氧胆酸钠通过MAPK/ERK1/2介导Bcl-2在Ser70位点的磷酸化引起人肝细胞癌的抗药

B细胞淋巴瘤-2（Bcl-2）是一种重要的抗凋亡蛋白质,在多种人类肿瘤中普遍过表达。甘氨鹅脱氧胆酸钠（GCDA）与消化道肿瘤的发生发展密切相关,并能介导肝癌细胞对化疗药物的抵抗。本文旨在探讨在GCDA介导的人肝细胞癌（HCC）耐药性中Bcl-2的作用及其机制。本研究以肝癌细胞系为研究对象,Western印迹结果显示,Bcl-2在多种肝癌细胞系中均有表达。设计靶向Bcl-2的siRNA沉默HCC细胞系内源性Bcl-2的表达,发现Bcl-2沉默之后促进了化疗药物5-FU介导的HCC细胞凋亡。机制上,GCDA可介导Bcl-2在Ser70位点的磷酸化,而Ser70位点的磷酸化能够被PD98059（MAPK/ERK1/2抑制剂）所抑制。构建huBcl2-WT和huBcl2-S70A真核表达载体,脂质体转染HCC细胞系。用Annexin V-FITC/PI流式细胞术检测凋亡细胞。结果显示,huBcl2-WT过表达能抑制5 FU介导的凋亡,S70位点失活突变成A后,Bcl-2的过表达不能抑制5-FU介导的凋亡。本研究提示,GCDA通过MAPK/ERK1/2通路介导的Bcl-2 Ser70位点的磷酸化,在肝癌细胞的存活和抗药中发挥重要作用。抑制Bcl-2能够促进化疗药物5-FU介导的HCC细胞凋亡,该结果为治疗GCDA介导的耐药性肝癌提供新的思路。