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We have probed the chromatin conformation of the G gamma-A gamma-delta-beta globin gene locus of K562 cells, a human hematopoietic cell line, with the enzyme pancreatic DNAse I. This enzyme preferentially digests genes in an active configuration. We have found that in K562 cells, which produce embryonic and fetal but not adult hemoglobins, both the active gamma and inactive beta genes are DNAse I sensitive. However, only the active gamma genes have DNAse I hypersensitive regions. The hypersensitive regions have been mapped to an area approximately 100 base pairs 5' to the G gamma and A gamma genes. 相似文献
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I Grummt 《Nucleic acids research》1981,9(22):6093-6102
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Differential transcription of Xenopus oocyte and somatic-type 5 S genes in a Xenopus oocyte extract 总被引:10,自引:0,他引:10
L Millstein P Eversole-Cire J Blanco J M Gottesfeld 《The Journal of biological chemistry》1987,262(35):17100-17110
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A replication fork barrier at the 3' end of yeast ribosomal RNA genes 总被引:51,自引:0,他引:51
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VSG gene 118 is transcribed from a cotransposed pol I-like promoter 总被引:31,自引:0,他引:31
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DNase I-hypersensitive sites in the galactose gene cluster of Saccharomyces cerevisiae. 总被引:9,自引:2,他引:7
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J H Proffitt 《Molecular and cellular biology》1985,5(6):1522-1524