On the spatial structure of a plant cell wall protein. Secondary structure of a cell wall protein fromAcetabularia

Summary The structure of a purified protein associated with the cell wall polysaccharides of the marine green algaeAcetabularia (Polyphysa) cliftonii has been studied by means of X-ray diffraction, infrared spectroscopy and circular dichroism. The homogeneous preparation of the cell wall protein has a molecular weight of 14,000, as determined by sodium-dodecylsulfate electrophoresis. Regular layer line reflections on the X-ray diffraction photographs suggest that a distinct order exists in the arrangement of the protein fibrils. Through infrared spectroscopy of thin aqueous films of the protein, as well as of the fibers, it was established that the -helical structure is predominant in the cell wall protein. The fibers crystallize in a hexagonal unit cell witha=14.5 Å and c=27.0 Å, at a water content of two molecules per residue. Increase in water content causes an increase in thea-axis, but without change in thec-direction, thus keeping the -helical conformation. Moreover the spectral data in the amide A, I, II, III, and IV-regions show that the cell wall protein has an ordered -helical conformation.     

Three-dimensional structure of a cell wall glycoprotein

Electron microscopy and computer image analysis have been used to determine the three-dimensional structure of the crystalline glycoprotein cell wall layer of the alga Lobomonas piriformis. Images of negatively stained specimens, tilted through a range of angles up to 70 °, were combined to give a map of the molecular envelope to a resolution of 2.0 nm. The cell wall layer consists of crystalline plates the centres and edges of which display distinctly different but isomorphous structures. A comparison of three-dimensional reconstructions of the two areas shows the difference probably to be due to a conformational change of one of the glycoprotein subunits. The structure consists of two sets of dimers composed of rod-shaped subunits which lie with their long axes approximately in the plane of the crystal. The centre-edge transition may have significance in the pathway of accretion of new subunits during cell wall growth.     

Formation of plant cell wall supramolecular structure

Plant cell wall is an example of a widespread natural supramolecular structure: its components are considered to be the most abundant organic compounds renewable by living organisms. Plant cell wall includes numerous components, mainly polysaccharidic; its formation is largely based on carbohydrate-carbohydrate interactions. In contrast to the extracellular matrix of most other organisms, the plant cell compartment located outside the plasma membrane is so structured that has been named “wall”. The present review summarizes data on the mechanisms of formation of this supramolecular structure and considers major difficulties and results of research. Existing approaches to the study of interactions between polysaccharides during plant cell wall formation have been analyzed, including: (i) characterization of the structure of natural polysaccharide complexes obtained during cell wall fractionation; (ii) analysis of the interactions between polysaccharides “at mixing in a tube”; (iii) study of the interactions between isolated individual plant cell wall matrix polysaccharides and microfibrils formed by cellulose-synthesizing microorganisms; and (iv) investigation of cell wall formation and modification directly in plant objects. The key stages in formation of plant cell wall supramolecular structure are defined and characterized as follows: (i) formation of cellulose microfibrils; (ii) interactions between matrix polysaccharides within Golgi apparatus substructures; (iii) interaction between matrix polysaccharides, newly secreted outside the plasma membrane, and cellulose microfibrils during formation of the latter; (iv) packaging of the formed complexes and individual polysaccharides in cell wall layers; and (v) modification of deposited cell wall layers.     

The structure and synthesis of the fungal cell wall

The fungal cell wall is a dynamic structure that protects the cell from changes in osmotic pressure and other environmental stresses, while allowing the fungal cell to interact with its environment. The structure and biosynthesis of a fungal cell wall is unique to the fungi, and is therefore an excellent target for the development of anti-fungal drugs. The structure of the fungal cell wall and the drugs that target its biosynthesis are reviewed. Based on studies in a number of fungi, the cell wall has been shown to be primarily composed of chitin, glucans, mannans and glycoproteins. The biosynthesis of the various components of the fungal cell wall and the importance of the components in the formation of a functional cell wall, as revealed through mutational analyses, are discussed. There is strong evidence that the chitin, glucans and glycoproteins are covalently cross-linked together and that the cross-linking is a dynamic process that occurs extracellularly.