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1.
《Molecular & cellular proteomics : MCP》2020,19(6):1005-1016
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- •Brain membrane protein extraction.
- •Protein prenylation.
- •Prenyl peptide capture and characterization by LC-MS/MS.
- •HCD and EThcD peptide fragmentation.
2.
《Molecular & cellular proteomics : MCP》2020,19(4):655-671
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- •Acute inhibition of growth-regulatory AGC-kinases Sch9, PKA and Ypk1.
- •Phospho-proteomic profiling of 6373 phsopho-sites.
- •Ypk1 regulates phospho-sites in RRxS/T-context and functionally overlaps with PKA.
- •Ypk1 and PKA activate trehalase activity of Nth1.
3.
《Molecular & cellular proteomics : MCP》2020,19(3):444-455
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- •Human spermatozoa possess cells of poor morphology that lack nuclear integrity.
- •These cells can be isolated by density separation.
- •Mass spectrometry reveals their nuclei contain excess protein.
- •TOP2A is a promising marker of this poor nuclear development.
4.
《Molecular & cellular proteomics : MCP》2020,19(7):1088-1103
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- •Rapid DIA-only library building with gas-phase fractionation.
- •Recommended DIA acquisition strategies with staggered windows and forbidden zones.
- •Optimized DIA instrument settings for several Thermo Orbitrap instruments.
- •Data analysis tutorial using open source DIA software.
5.
《Molecular & cellular proteomics : MCP》2020,19(5):828-838
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- •Higher AGC significantly improves quantitation quality in single-cell analysis.
- •The boosting-to-sample ratio should be carefully evaluated and optimized.
- •iBASIL allows for precise quantitation of 1,500 proteins from 104 AML single cells.
- •iBASIL recapitulates major biological differences in different AML single cells.
6.
《Molecular & cellular proteomics : MCP》2020,19(3):456-466
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- •Urinary proteomes of patients with recurrent UTI, renal scarring, and VUR.
- •80 proteins differentially expressed, compared to healthy controls.
- •62 proteins may be indicative of susceptibility for UTI.
- •Altered acute phase response, extracellular matrix and carbohydrate metabolism.
7.
Nataly Mancette Rijensky Netta R. Blondheim Shraga Eilon Barnea Nir Peled Eli Rosenbaum Aron Popovtzer Solomon M. Stemmer Alejandro Livoff Mark Shlapobersky Neta Moskovits Dafna Perry Eitan Rubin Itzhak Haviv Arie Admon 《Molecular & cellular proteomics : MCP》2020,19(8):1360-1374
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- •Sufficient tumor tissues are often unavailable large HLA peptidome discovery.
- •Using patient derived xenograft (PDX) tumors can overcome this limitation.
- •The large PDX HLA peptidomes expand significantly those of the original biopsies.
- •The HLA peptidomes of the PDX tumors included many tumor antigens.
8.
《Molecular & cellular proteomics : MCP》2020,19(7):1193-1208
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- •cGAS acetylations and phosphorylations under basal and immune-stimulated states.
- •K384 and K414 acetylations and S305 phosphorylation inhibit cGAS-mediated apoptosis.
- •Acetylation at K198 stimulates cGAS-dependent interferon signaling.
- •K198 acetylation is decreased upon herpesvirus infection.
9.
《Molecular & cellular proteomics : MCP》2020,19(7):1120-1131
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- •Quantitative proteomics of isolated lysosomes, autophagosomes and proteasomes.
- •Pharmacological inhibition of proteasomes leads to their accumulation within lysosomes.
- •Inhibition of classical autophagy pathways cannot completely block this process.
- •Known autophagy adaptor proteins are not involved.
10.
《Molecular & cellular proteomics : MCP》2020,19(3):540-553
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- •Repeatable quantification of 200 proteins in dried blood spots.
- •Determined lower limit of quantification, repeatability, parallelism and stability.
- •Protein stability in DBS stored at ambient temperatures for up to 2 months.
- •Concentration ranges for 200 proteins in 20 healthy individuals.
11.
《Molecular & cellular proteomics : MCP》2020,19(4):574-588
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- •SILAC-based protein quantification of OA hBMSCs undergoing chondrogenesis.
- •Spatially-resolved metabolomics by MSI of hBMSCs in chondrogenic differentiation.
- •Differential metabolic pathways involved in OA compared to control hBMSCs.
- •UDP-glucuronic acid/UDP-GlcNAc synthesis is decreased in chondrogenic OA hBMSCs.
12.
《Molecular & cellular proteomics : MCP》2020,19(5):900-912
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- •Affinity-based proteomics of infected macrophage cells.
- •Salmonella-modified membranes exhibit host-specific composition.
- •Proteome differences explain some host-dependent pathophysiological differences.
13.
《Molecular & cellular proteomics : MCP》2020,19(4):690-700
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- •Two molecular groups in anal squamous carcinoma according proteomic profile.
- •Differences in possible targeted processes such as metabolism or immune response.
- •Different percentage of tumor lymphocyte infiltration.
- •Difference in the frequency of ATM variants, related to PPAR inhibitors.
14.
《Molecular & cellular proteomics : MCP》2020,19(3):478-489
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- •Comprehensive molecular profiling of cutaneous and cerebellar metastasis variants.
- •Identification of differentially regulated metastasis-associated molecules.
- •Evidence for individually distinct patterns of metastasis-associated molecules.
- •Highlighting the evident need for establishing meta-analyses strategies.
15.
《Molecular & cellular proteomics : MCP》2020,19(5):744-756
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- •Signaling networks can be highly heterogeneous across cells in a tissue.
- •Various technologies allow analyzing signaling networks at single-cell resolution.
- •The advantages and limitations of each single-cell approach are summarized.
- •Confounding factors in single-cell signaling network analysis are discussed.
16.
《Molecular & cellular proteomics : MCP》2020,19(6):1047-1057
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- •DEqMS is a method for statistical analysis of quantitative MS-data.
- •Variance estimates based on the actual MS-data structure.
- •Improved statistical power and accuracy in protein differential analysis.
- •DEqMS is available as a user-friendly R package in Bioconductor.
17.
《Molecular & cellular proteomics : MCP》2020,19(6):928-943
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- •EGFR-TKI molecular response profiling covering 10138 proteins and 13486 mRNAs.
- •EGFR-TKI combination therapy screen using a library of 528 compounds.
- •Several new candidate EGFR-TKI escape mechanisms and combination therapy targets.
- •Combined targeting of the oncogene BCL6 and EGFR results in synergy in NSCLC cells.
18.
《Molecular & cellular proteomics : MCP》2020,19(6):1058-1069
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- •Highly parallelizable 4D feature detection in ion mobility enhanced shotgun proteomics.
- •Multidimensional non-linear mass, retention time and ion mobility recalibration.
- •Collision cross section aware matching between runs.
- •Label-free quantification of ion mobility MS data.
19.
《Molecular & cellular proteomics : MCP》2020,19(6):916-927
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- •Summarize the development of functional protein microarray.
- •Application of functional proteome microarray in basic research.
- •Application of functional proteome microarray in translational research.
- •Fabrication of functional membrane protein array using virion display method.
20.
Eduard Hofsetz Fatih Demir Karolina Szczepanowska Alexandra Kukat Jayachandran N. Kizhakkedathu Aleksandra Trifunovic Pitter F. Huesgen 《Molecular & cellular proteomics : MCP》2020,19(8):1330-1345
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- •Mitochondrial heart N terminome shows aminopeptidase processing after MTS cleavage.
- •CLPP-deficiency alters protein processing patterns in mouse heart mitochondria.
- •Candidate substrates identified by N termini accumulation and interaction with inactive ClpXP.
- •UQCRC1, HSPA9 and OAT validated biochemically as high confidence ClpXP substrates.