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1.
《Molecular & cellular proteomics : MCP》2020,19(3):518-528
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- •Discovery of peptide biomarker candidates of respiratory tract pathogens S. pneumoniae, H. influenzae, M. catarrhalis and S. aureus as target pathogens.
- •Peptide biomarker candidates were experimentally verified in clinical samples.
- •Targeted MS using promising peptide biomarker candidates shown as proof-of-concept.
2.
《Molecular & cellular proteomics : MCP》2020,19(6):916-927
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- •Summarize the development of functional protein microarray.
- •Application of functional proteome microarray in basic research.
- •Application of functional proteome microarray in translational research.
- •Fabrication of functional membrane protein array using virion display method.
3.
《Molecular & cellular proteomics : MCP》2020,19(2):294-307
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- •Quantitative proteomes of the cellular surface changes induced by mTORC1 signaling.
- •Hit validation in human cancer cell lines and biopsies.
- •Functional studies showing new drug targets to which cancer cells with hyperactive mTORC1 may be addicted.
- •A new paradigm for drug development, namely targeting cell surface proteins regulated by mTORC1.
4.
Elez D. Vainer Juliane Kania-Almog Ghadeer Zatara Yishai Levin Gilad W. Vainer 《Molecular & cellular proteomics : MCP》2020,19(10):1619-1631
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- •TOP: robust, bio-friendly FFPE proteome extraction method with less fixation bias.
- •Proteome of MSI-H colorectal cancer identifies immunobiology key elements.
- •MSI-H tumor displays an “INFg-STAT1 centric signature”.
- •Long-term IFNg induction In-vitro mimicks MSI-H signature.
5.
《Molecular & cellular proteomics : MCP》2020,19(11):1749-1759
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- •In-depth profiling of the serum proteome in early-stage COVID-19 patients.
- •A landscape of inflammation and immune signaling related to the SARS-CoV-2 infection.
- •CCL2 and CXCL10 medicated cytokine signaling pathways may correlate with neutrophil and lymphocyte respectively.
6.
《Molecular & cellular proteomics : MCP》2020,19(6):928-943
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- •EGFR-TKI molecular response profiling covering 10138 proteins and 13486 mRNAs.
- •EGFR-TKI combination therapy screen using a library of 528 compounds.
- •Several new candidate EGFR-TKI escape mechanisms and combination therapy targets.
- •Combined targeting of the oncogene BCL6 and EGFR results in synergy in NSCLC cells.
7.
《Molecular & cellular proteomics : MCP》2020,19(2):326-343
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- •NCMR is crucial for substrate recognition and activity regulation.
- •MASTL conserves a cryptic C-Lobe in the non-conserved middle region.
- •MASTL450 containing the cryptic C-lobe is observed in cancer cell lines.
- •Key phosphorylation sites for MASTL provide an activation model.
8.
Fei Fang Qun Zhao Huiying Chu Mingwei Liu Baofeng Zhao Zhen Liang Lihua Zhang Guohui Li Liming Wang Jun Qin Yukui Zhang 《Molecular & cellular proteomics : MCP》2020,19(10):1724-1737
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- •Mechanistic insights into ionic liquids and proteins at molecular level.
- •Extractants prescreen for proteome analysis with MD simulation system.
- •A loss-less sample preparation method developed for in-depth proteome profiling.
- •Over 3,300 proteins were confidently identified from 1,000 HeLa cells in a 1 h run.
- •Label-free quantitative proteome analysis of human liver cancer tissues.
9.
《Molecular & cellular proteomics : MCP》2020,19(3):432-443
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- •Validation of an omic method for antigen identification using LC-MS/MS.
- •Validation of accuracy, precision, specificity, limit of detection and robustness.
- •Validation according to the current FDA and EMA guidelines.
10.
Nataly Mancette Rijensky Netta R. Blondheim Shraga Eilon Barnea Nir Peled Eli Rosenbaum Aron Popovtzer Solomon M. Stemmer Alejandro Livoff Mark Shlapobersky Neta Moskovits Dafna Perry Eitan Rubin Itzhak Haviv Arie Admon 《Molecular & cellular proteomics : MCP》2020,19(8):1360-1374
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- •Sufficient tumor tissues are often unavailable large HLA peptidome discovery.
- •Using patient derived xenograft (PDX) tumors can overcome this limitation.
- •The large PDX HLA peptidomes expand significantly those of the original biopsies.
- •The HLA peptidomes of the PDX tumors included many tumor antigens.
11.
Alison M. Kurimchak Vikas Kumar Carlos Herrera-Montvez Katherine J. Johnson Nishi Srivastava Karthik Davarajan Suraj Peri Kathy Q. Cai Gina M. Mantia-Smaldone James S. Duncan 《Molecular & cellular proteomics : MCP》2020,19(12):2068-2090
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- •SRPK1 is overexpressed in endometrial cancer and associated with poor survival.
- •SRPK1 promotes endometrial cancer cell growth under nutrient-deprived conditions.
- •Activation of EGFR-IGF1R-AKT signaling promotes resistance to SRPK1 inhibitors.
- •Co-targeting SRPK1 and EGFR-IGF1R synergize blocking endometrial cancer cell growth.
12.
《Molecular & cellular proteomics : MCP》2020,19(4):624-639
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- •Used affinity-enrichable, isotopically coded, and MS-cleavable crosslinker.
- •Targeted acquisition strategy based on isotopic-coding described and evaluated.
- •Novel data analysis pipeline developed provides improved crosslink identification.
- •Large dataset reveals hundreds of mitochondrial protein-protein interactions.
13.
《Molecular & cellular proteomics : MCP》2020,19(4):690-700
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- •Two molecular groups in anal squamous carcinoma according proteomic profile.
- •Differences in possible targeted processes such as metabolism or immune response.
- •Different percentage of tumor lymphocyte infiltration.
- •Difference in the frequency of ATM variants, related to PPAR inhibitors.
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15.
《Molecular & cellular proteomics : MCP》2020,19(2):375-389
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- •CD73 is one of the most upregulated proteins in the radioresistant cells.
- •CD73 upregulation confers radioresistance and irradiation-induced apoptosis.
- •CD73 confers radioresistance potentially through inactivating protein BAD.
- •Elevated CD73 is required for maintaining the resistant cells in a mesenchymal state.
16.
《Molecular & cellular proteomics : MCP》2020,19(1):31-49
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- •Immunopeptidomics bears the potential to link diseases to antigen representation.
- •We suggest to achieve this by analyzing the immunopeptidomes of cohorts of patients.
- •Current mass spectrometry-based techniques to analyze immunopeptidomes are described.
- •We term the proposed approach “Immunopeptidome-wide association studies” (IWAS).
17.
《Molecular & cellular proteomics : MCP》2020,19(5):884-899
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- •pY phosphoproteomes and dedicated ranking analyses for 16 AML cell lines.
- •RTK drivers, 6 mutant cell lines confirmed, identification for 4 more cell lines.
- •MAPK1/3 phosphorylation for cell lines without TK driver, indicating RAS mutation.
- •Drug target space phosphorylation correlates with drug IC50s in specific cell lines.
18.
Svenja Wiechmann Elena Saupp Daniela Schilling Stephanie Heinzlmeir Günter Schneider Roland M. Schmid Stephanie E. Combs Bernhard Kuster Sophie Dobiasch 《Molecular & cellular proteomics : MCP》2020,19(10):1649-1663
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- •Proteomes and phosphoproteomes of radiosensitive and radioresistant PDAC cell lines.
- •Common activation of DDR is proven by ATM activity on known and novel substrates.
- •Resistant cells bear raised NQO1 expression, actin dynamics including FAK activity.
- •Inhibitors of CHEK Rabusertib and FAK Defactinib radiosensitize PDAC cells.
19.
《Molecular & cellular proteomics : MCP》2020,19(2):261-277
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- •We used phosphoproteomics to reveal the underlying mechanisms of drug synergy on EGFR and ROCK co-inhibition in TNBC cells.
- •EGFR inhibition alone induces autophagy activation in TNBC cells as a cytoprotective mechanism.
- •Combinatorial treatment leads to impaired autophagic flux resulting in a strong accumulation of autophagic vacuoles.
- •We hypothesize that ROCKi-induced cytoskeletal changes impair autophagosome clearance ultimately leading to cell death.
20.
《Molecular & cellular proteomics : MCP》2020,19(6):1005-1016
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- •Brain membrane protein extraction.
- •Protein prenylation.
- •Prenyl peptide capture and characterization by LC-MS/MS.
- •HCD and EThcD peptide fragmentation.