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1.
《Molecular & cellular proteomics : MCP》2020,19(2):362-374
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- •Monocytes are isolated from single donors after apheresis.
- •Monocytes process CD16a and CD32a N-glycosylation differently by site and donor.
- •CD16a with hybrid and oligomannose type N-glycans bind IgG1 Fc with stronger affinity than complex type.
2.
《Molecular & cellular proteomics : MCP》2020,19(3):529-539
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- •N-glycosylation site analysis of the hemipteran pest insect Nilaparvata lugens.
- •Differential N-glycosylation of proteins is observed between male and female adults.
- •Sex-specific glycoproteins are involved in insect reproduction.
3.
《Molecular & cellular proteomics : MCP》2020,19(11):1910-1920
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- •PRMT5 glutathionylation is increased in aged mice or under oxidative stress.
- •Deglutathionylation of PRMT5 is catalyzed by glutaredoxin-1.
- •PRMT5 glutathionylation decreases its methyltransferase activity.
- •PRMT5 glutathionylation results in G2/M arrest and inhibits cell proliferation.
4.
《Molecular & cellular proteomics : MCP》2020,19(5):900-912
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- •Affinity-based proteomics of infected macrophage cells.
- •Salmonella-modified membranes exhibit host-specific composition.
- •Proteome differences explain some host-dependent pathophysiological differences.
5.
《Molecular & cellular proteomics : MCP》2020,19(5):828-838
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- •Higher AGC significantly improves quantitation quality in single-cell analysis.
- •The boosting-to-sample ratio should be carefully evaluated and optimized.
- •iBASIL allows for precise quantitation of 1,500 proteins from 104 AML single cells.
- •iBASIL recapitulates major biological differences in different AML single cells.
6.
《Molecular & cellular proteomics : MCP》2020,19(1):114-127
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- •Quantitative proteomics and machine learning to study plasma biomarkers in HCM.
- •Six peptides are increased in plasma of LVH+ HCM compared to controls.
- •Peptide biomarkers correlate with imaging markers of phenotype severity.
- •Peptide biomarkers correlate with the estimated sudden cardiac death risk.
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8.
《Molecular & cellular proteomics : MCP》2020,19(3):456-466
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- •Urinary proteomes of patients with recurrent UTI, renal scarring, and VUR.
- •80 proteins differentially expressed, compared to healthy controls.
- •62 proteins may be indicative of susceptibility for UTI.
- •Altered acute phase response, extracellular matrix and carbohydrate metabolism.
9.
《Molecular & cellular proteomics : MCP》2020,19(6):1005-1016
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- •Brain membrane protein extraction.
- •Protein prenylation.
- •Prenyl peptide capture and characterization by LC-MS/MS.
- •HCD and EThcD peptide fragmentation.
10.
zge Karayel Francesca Tonelli Sebastian Virreira Winter Phillip E. Geyer Ying Fan Esther M. Sammler Dario R. Alessi Martin Steger Matthias Mann 《Molecular & cellular proteomics : MCP》2020,19(9):1546-1560
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- •MS-based clinical assay that accurately determines phospho Rab10 occupancy.
- •Stable isotope labeled phosphopeptide injected as a standard with endogenous tryptic phospho Rab peptide for accurate ratio determination.
- •Determination of pRab levels in neutrophils of Parkinson disease patients.
- •Relevance of pRab levels as marker of PD.
11.
《Molecular & cellular proteomics : MCP》2020,19(1):31-49
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- •Immunopeptidomics bears the potential to link diseases to antigen representation.
- •We suggest to achieve this by analyzing the immunopeptidomes of cohorts of patients.
- •Current mass spectrometry-based techniques to analyze immunopeptidomes are described.
- •We term the proposed approach “Immunopeptidome-wide association studies” (IWAS).
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13.
《Molecular & cellular proteomics : MCP》2020,19(4):690-700
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- •Two molecular groups in anal squamous carcinoma according proteomic profile.
- •Differences in possible targeted processes such as metabolism or immune response.
- •Different percentage of tumor lymphocyte infiltration.
- •Difference in the frequency of ATM variants, related to PPAR inhibitors.
14.
《Molecular & cellular proteomics : MCP》2020,19(5):744-756
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- •Signaling networks can be highly heterogeneous across cells in a tissue.
- •Various technologies allow analyzing signaling networks at single-cell resolution.
- •The advantages and limitations of each single-cell approach are summarized.
- •Confounding factors in single-cell signaling network analysis are discussed.
15.
《Molecular & cellular proteomics : MCP》2020,19(6):916-927
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- •Summarize the development of functional protein microarray.
- •Application of functional proteome microarray in basic research.
- •Application of functional proteome microarray in translational research.
- •Fabrication of functional membrane protein array using virion display method.
16.
《Molecular & cellular proteomics : MCP》2020,19(5):839-851
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- •A detailed investigation of the protein extraction step from FFPE tissue is shown.
- •Acidification during peptide wash increased peptide recovery of the SP3 method.
- •LCM of substantia nigra enriched neuron-specific proteins including TH.
- •>5,600 proteins were quantified using 3000 cells per sample from substantia nigra.
17.
《Molecular & cellular proteomics : MCP》2020,19(6):928-943
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- •EGFR-TKI molecular response profiling covering 10138 proteins and 13486 mRNAs.
- •EGFR-TKI combination therapy screen using a library of 528 compounds.
- •Several new candidate EGFR-TKI escape mechanisms and combination therapy targets.
- •Combined targeting of the oncogene BCL6 and EGFR results in synergy in NSCLC cells.
18.
《Molecular & cellular proteomics : MCP》2020,19(2):421-430
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- •Modern DIA methods contain high quality MS1 and MS2.
- •We developed a statistical procedure incorporating MS1 and MS2.
- •Benchmarking, the combined method outperformed the individual use of MS1 or MS2.
19.
《Molecular & cellular proteomics : MCP》2020,19(6):1058-1069
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- •Highly parallelizable 4D feature detection in ion mobility enhanced shotgun proteomics.
- •Multidimensional non-linear mass, retention time and ion mobility recalibration.
- •Collision cross section aware matching between runs.
- •Label-free quantification of ion mobility MS data.
20.
《Molecular & cellular proteomics : MCP》2020,19(3):478-489
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- •Comprehensive molecular profiling of cutaneous and cerebellar metastasis variants.
- •Identification of differentially regulated metastasis-associated molecules.
- •Evidence for individually distinct patterns of metastasis-associated molecules.
- •Highlighting the evident need for establishing meta-analyses strategies.