共查询到20条相似文献,搜索用时 156 毫秒
1.
Yi-Han Lin Maryann P. Platt Haiyan Fu Yuan Gui Yanlin Wang Norberto Gonzalez-Juarbe Dong Zhou Yanbao Yu 《Molecular & cellular proteomics : MCP》2020,19(12):2030-2047
- Download : Download high-res image (197KB)
- Download : Download full-size image
- •Cecal Ligation Puncture (CLP) mouse model to study sepsis-induced kidney disease.
- •Quantitative global proteome and phosphoproteome profiling of mouse kidneys.
- •Highly significant candidate markers for onset and progression of AKI to CKD.
- •Mechanistic insights into sepsis-associated kidney injuries.
2.
《Molecular & cellular proteomics : MCP》2020,19(3):540-553
- Download : Download high-res image (213KB)
- Download : Download full-size image
- •Repeatable quantification of 200 proteins in dried blood spots.
- •Determined lower limit of quantification, repeatability, parallelism and stability.
- •Protein stability in DBS stored at ambient temperatures for up to 2 months.
- •Concentration ranges for 200 proteins in 20 healthy individuals.
3.
《Molecular & cellular proteomics : MCP》2020,19(3):444-455
- Download : Download high-res image (183KB)
- Download : Download full-size image
- •Human spermatozoa possess cells of poor morphology that lack nuclear integrity.
- •These cells can be isolated by density separation.
- •Mass spectrometry reveals their nuclei contain excess protein.
- •TOP2A is a promising marker of this poor nuclear development.
4.
5.
《Molecular & cellular proteomics : MCP》2020,19(3):456-466
- Download : Download high-res image (207KB)
- Download : Download full-size image
- •Urinary proteomes of patients with recurrent UTI, renal scarring, and VUR.
- •80 proteins differentially expressed, compared to healthy controls.
- •62 proteins may be indicative of susceptibility for UTI.
- •Altered acute phase response, extracellular matrix and carbohydrate metabolism.
6.
《Molecular & cellular proteomics : MCP》2020,19(4):690-700
- Download : Download high-res image (209KB)
- Download : Download full-size image
- •Two molecular groups in anal squamous carcinoma according proteomic profile.
- •Differences in possible targeted processes such as metabolism or immune response.
- •Different percentage of tumor lymphocyte infiltration.
- •Difference in the frequency of ATM variants, related to PPAR inhibitors.
7.
《Molecular & cellular proteomics : MCP》2020,19(7):1076-1087
- Download : Download high-res image (233KB)
- Download : Download full-size image
- •Organelle profiling maps capture localizations of 1000s of proteins in one experiment.
- •Comparing maps +/− perturbation reveals disease mechanisms & cellular responses.
- •A conceptual guide to planning and interpreting organellar profiling experiments.
- •A cross-study consensus set of human organellar marker proteins.
8.
《Molecular & cellular proteomics : MCP》2020,19(5):744-756
- Download : Download high-res image (204KB)
- Download : Download full-size image
- •Signaling networks can be highly heterogeneous across cells in a tissue.
- •Various technologies allow analyzing signaling networks at single-cell resolution.
- •The advantages and limitations of each single-cell approach are summarized.
- •Confounding factors in single-cell signaling network analysis are discussed.
9.
10.
《Molecular & cellular proteomics : MCP》2020,19(4):574-588
- Download : Download high-res image (221KB)
- Download : Download full-size image
- •SILAC-based protein quantification of OA hBMSCs undergoing chondrogenesis.
- •Spatially-resolved metabolomics by MSI of hBMSCs in chondrogenic differentiation.
- •Differential metabolic pathways involved in OA compared to control hBMSCs.
- •UDP-glucuronic acid/UDP-GlcNAc synthesis is decreased in chondrogenic OA hBMSCs.
11.
《Molecular & cellular proteomics : MCP》2020,19(2):294-307
- Download : Download high-res image (227KB)
- Download : Download full-size image
- •Quantitative proteomes of the cellular surface changes induced by mTORC1 signaling.
- •Hit validation in human cancer cell lines and biopsies.
- •Functional studies showing new drug targets to which cancer cells with hyperactive mTORC1 may be addicted.
- •A new paradigm for drug development, namely targeting cell surface proteins regulated by mTORC1.
12.
《Molecular & cellular proteomics : MCP》2020,19(2):326-343
- Download : Download high-res image (140KB)
- Download : Download full-size image
- •NCMR is crucial for substrate recognition and activity regulation.
- •MASTL conserves a cryptic C-Lobe in the non-conserved middle region.
- •MASTL450 containing the cryptic C-lobe is observed in cancer cell lines.
- •Key phosphorylation sites for MASTL provide an activation model.
13.
《Molecular & cellular proteomics : MCP》2020,19(7):1161-1178
- Download : Download high-res image (250KB)
- Download : Download full-size image
- •XL-MS reveals new PPIs in yeast mitochondria under glycerol and glucose condition.
- •Significant but limited results from quantitative XL-MS experiments.
- •Ndi1 participates in a CIII2CIV2 respiratory supercomplex.
- •Min8 promotes assembly of Cox12 into an intermediate complex IV.
14.
Eduard Hofsetz Fatih Demir Karolina Szczepanowska Alexandra Kukat Jayachandran N. Kizhakkedathu Aleksandra Trifunovic Pitter F. Huesgen 《Molecular & cellular proteomics : MCP》2020,19(8):1330-1345
- Download : Download high-res image (211KB)
- Download : Download full-size image
- •Mitochondrial heart N terminome shows aminopeptidase processing after MTS cleavage.
- •CLPP-deficiency alters protein processing patterns in mouse heart mitochondria.
- •Candidate substrates identified by N termini accumulation and interaction with inactive ClpXP.
- •UQCRC1, HSPA9 and OAT validated biochemically as high confidence ClpXP substrates.
15.
《Molecular & cellular proteomics : MCP》2020,19(11):1860-1875
- Download : Download high-res image (101KB)
- Download : Download full-size image
- •During sperm capacitation, 6 proteins become de-silyated, whilst one becomes silyated.
- •Loss of sialylated from Endothelial lipase results in reduction of activity.
- •Silyation of N612 on Aconitase causes loss of activity.
- •Silyation of Aconitase may explain the switch from oxidative phosphorylation to glycolysis during capacitation.
16.
Elez D. Vainer Juliane Kania-Almog Ghadeer Zatara Yishai Levin Gilad W. Vainer 《Molecular & cellular proteomics : MCP》2020,19(10):1619-1631
- Download : Download high-res image (249KB)
- Download : Download full-size image
- •TOP: robust, bio-friendly FFPE proteome extraction method with less fixation bias.
- •Proteome of MSI-H colorectal cancer identifies immunobiology key elements.
- •MSI-H tumor displays an “INFg-STAT1 centric signature”.
- •Long-term IFNg induction In-vitro mimicks MSI-H signature.
17.
Prashali Bansal Johannes Madlung Kristina Schaaf Boris Macek Fulvia Bono 《Molecular & cellular proteomics : MCP》2020,19(9):1485-1502
- Download : Download high-res image (92KB)
- Download : Download full-size image
- •Label-free and dimethyl labeling MS analysis of 6 RBPs from Drosophila ovaries.
- •Functionally related RBPs show overlapping proteomes.
- •Selective co-purification of splicing factors and translational regulators.
- •Validation of 26 novel interactions by co-immunoprecipitation.
18.
《Molecular & cellular proteomics : MCP》2020,19(6):928-943
- Download : Download high-res image (422KB)
- Download : Download full-size image
- •EGFR-TKI molecular response profiling covering 10138 proteins and 13486 mRNAs.
- •EGFR-TKI combination therapy screen using a library of 528 compounds.
- •Several new candidate EGFR-TKI escape mechanisms and combination therapy targets.
- •Combined targeting of the oncogene BCL6 and EGFR results in synergy in NSCLC cells.
19.
《Molecular & cellular proteomics : MCP》2020,19(4):624-639
- Download : Download high-res image (248KB)
- Download : Download full-size image
- •Used affinity-enrichable, isotopically coded, and MS-cleavable crosslinker.
- •Targeted acquisition strategy based on isotopic-coding described and evaluated.
- •Novel data analysis pipeline developed provides improved crosslink identification.
- •Large dataset reveals hundreds of mitochondrial protein-protein interactions.
20.
《Molecular & cellular proteomics : MCP》2020,19(5):884-899
- Download : Download high-res image (172KB)
- Download : Download full-size image
- •pY phosphoproteomes and dedicated ranking analyses for 16 AML cell lines.
- •RTK drivers, 6 mutant cell lines confirmed, identification for 4 more cell lines.
- •MAPK1/3 phosphorylation for cell lines without TK driver, indicating RAS mutation.
- •Drug target space phosphorylation correlates with drug IC50s in specific cell lines.