Molecular Dynamics Simulation-assisted Ionic Liquid Screening for Deep Coverage Proteome Analysis

1. Download : Download high-res image (123KB)
2. Download : Download full-size image

Highlights

• •Mechanistic insights into ionic liquids and proteins at molecular level.
• •Extractants prescreen for proteome analysis with MD simulation system.
• •A loss-less sample preparation method developed for in-depth proteome profiling.
• •Over 3,300 proteins were confidently identified from 1,000 HeLa cells in a 1 h run.
• •Label-free quantitative proteome analysis of human liver cancer tissues.

     

Developments and Applications of Functional Protein Microarrays

1. Download : Download high-res image (222KB)
2. Download : Download full-size image

Highlights

• •Summarize the development of functional protein microarray.
• •Application of functional proteome microarray in basic research.
• •Application of functional proteome microarray in translational research.
• •Fabrication of functional membrane protein array using virion display method.

     

A Compact Quadrupole-Orbitrap Mass Spectrometer with FAIMS Interface Improves Proteome Coverage in Short LC Gradients

1. Download : Download high-res image (158KB)
2. Download : Download full-size image

Highlights

• •Increased proteome coverage with Orbitrap Exploris 480 MS and FAIMS using single compensation voltages and short LC gradients.
• •Towards single-cell proteomics with high-sensitivity analysis of 5 ng HeLa with more than 1,000 proteins identified in 5 minutes using FAIMS and DIA.
• •Deep proteome profiling across twelve rat organs tissues by label-free quantitation using DIA compared to TMT-multiplexing and turboTMT acquisition using phi-SDM.
• •Rapid and sensitive phosphoproteomics with automated enrichment using Ti-IMAC magnetic beads and direct DIA analysis.

     

Dysregulation of Exosome Cargo by Mutant Tau Expressed in Human-induced Pluripotent Stem Cell (iPSC) Neurons Revealed by Proteomics Analyses

1. Download : Download high-res image (170KB)
2. Download : Download full-size image

Highlights

• •Mutant Tau dysregulates exosome cargo generated by human iPSC neurons.
• •Expression of P301L and V337M mutations of Tau in human iPSC neurons result in recruitment of distinct proteins to exosomes, and absence of control proteins to exosomes.
• •Tau mutations of FTDP-17 result in up- and downregulation of exosome proteins.
• •Altered proteome cargo of mTau exosomes may be involved in Tau pathogenesis in vivo in brain.

     

Arginine in C9ORF72 Dipolypeptides Mediates Promiscuous Proteome Binding and Multiple Modes of Toxicity

1. Download : Download high-res image (208KB)
2. Download : Download full-size image

Highlights

• •Quantitative proteome interactions with 5 different C9ORF72 dipolypeptides (DPRs).
• •The arg-rich DPRs promiscuously bound to the proteome compared with the other DPRs.
• •Long repeat lengths of arg-rich DPRs, but not short lengths, stalled ribosomes.
• •The arg-rich DPRs also reduced arginine methylation and actin cytoskeleton assembly.

     

Quantitative Profiling of the Human Substantia Nigra Proteome from Laser-capture Microdissected FFPE Tissue

1. Download : Download high-res image (225KB)
2. Download : Download full-size image

Highlights

• •A detailed investigation of the protein extraction step from FFPE tissue is shown.
• •Acidification during peptide wash increased peptide recovery of the SP3 method.
• •LCM of substantia nigra enriched neuron-specific proteins including TH.
• •>5,600 proteins were quantified using 3000 cells per sample from substantia nigra.

     

Characterizing Patients with Recurrent Urinary Tract Infections in Vesicoureteral Reflux: A Pilot Study of the Urinary Proteome

1. Download : Download high-res image (207KB)
2. Download : Download full-size image

Highlights

• •Urinary proteomes of patients with recurrent UTI, renal scarring, and VUR.
• •80 proteins differentially expressed, compared to healthy controls.
• •62 proteins may be indicative of susceptibility for UTI.
• •Altered acute phase response, extracellular matrix and carbohydrate metabolism.

     

Global Proteome and Phosphoproteome Characterization of Sepsis-induced Kidney Injury

1. Download : Download high-res image (197KB)
2. Download : Download full-size image

Highlights

• •Cecal Ligation Puncture (CLP) mouse model to study sepsis-induced kidney disease.
• •Quantitative global proteome and phosphoproteome profiling of mouse kidneys.
• •Highly significant candidate markers for onset and progression of AKI to CKD.
• •Mechanistic insights into sepsis-associated kidney injuries.

     

Concentration Determination of >200 Proteins in Dried Blood Spots for Biomarker Discovery and Validation

1. Download : Download high-res image (213KB)
2. Download : Download full-size image

Highlights

• •Repeatable quantification of 200 proteins in dried blood spots.
• •Determined lower limit of quantification, repeatability, parallelism and stability.
• •Protein stability in DBS stored at ambient temperatures for up to 2 months.
• •Concentration ranges for 200 proteins in 20 healthy individuals.

     

Agonists of Orally Expressed TRP Channels Stimulate Salivary Secretion and Modify the Salivary Proteome

1. Download : Download high-res image (108KB)
2. Download : Download full-size image

Highlights

• •Salivary secretion was increased by mouth rinsing with TRP channel agonists.
• •The salivary proteome varied over time and was changed by TRP channel stimulation.
• •Immunoreactive Cystatin S was increased in saliva after TRPV1 stimulation.

     

Glutathionylation Decreases Methyltransferase Activity of PRMT5 and Inhibits Cell Proliferation

1. Download : Download high-res image (194KB)
2. Download : Download full-size image

Highlights

• •PRMT5 glutathionylation is increased in aged mice or under oxidative stress.
• •Deglutathionylation of PRMT5 is catalyzed by glutaredoxin-1.
• •PRMT5 glutathionylation decreases its methyltransferase activity.
• •PRMT5 glutathionylation results in G2/M arrest and inhibits cell proliferation.

     

Novel Proteome Extraction Method Illustrates a Conserved Immunological Signature of MSI-H Colorectal Tumors

1. Download : Download high-res image (249KB)
2. Download : Download full-size image

Highlights

• •TOP: robust, bio-friendly FFPE proteome extraction method with less fixation bias.
• •Proteome of MSI-H colorectal cancer identifies immunobiology key elements.
• •MSI-H tumor displays an “INFg-STAT1 centric signature”.
• •Long-term IFNg induction In-vitro mimicks MSI-H signature.

     

Organellar Maps Through Proteomic Profiling – A Conceptual Guide

1. Download : Download high-res image (233KB)
2. Download : Download full-size image

Highlights

• •Organelle profiling maps capture localizations of 1000s of proteins in one experiment.
• •Comparing maps +/− perturbation reveals disease mechanisms & cellular responses.
• •A conceptual guide to planning and interpreting organellar profiling experiments.
• •A cross-study consensus set of human organellar marker proteins.

     

An Improved Boosting to Amplify Signal with Isobaric Labeling (iBASIL) Strategy for Precise Quantitative Single-cell Proteomics

1. Download : Download high-res image (162KB)
2. Download : Download full-size image

Highlights

• •Higher AGC significantly improves quantitation quality in single-cell analysis.
• •The boosting-to-sample ratio should be carefully evaluated and optimized.
• •iBASIL allows for precise quantitation of 1,500 proteins from 104 AML single cells.
• •iBASIL recapitulates major biological differences in different AML single cells.

     

Serum Protein Profiling Reveals a Landscape of Inflammation and Immune Signaling in Early-stage COVID-19 Infection

1. Download : Download high-res image (353KB)
2. Download : Download full-size image

Highlights

• •In-depth profiling of the serum proteome in early-stage COVID-19 patients.
• •A landscape of inflammation and immune signaling related to the SARS-CoV-2 infection.
• •CCL2 and CXCL10 medicated cytokine signaling pathways may correlate with neutrophil and lymphocyte respectively.

     

Investigation of Inter- and Intratumoral Heterogeneity of Glioblastoma Using TOF-SIMS

1. Download : Download high-res image (127KB)
2. Download : Download full-size image

Highlights

• •TOF-SIMS allows to visualize normal brain and tumor border.
• •Glioma samples can be subdivided into clinically relevant groups by TOF-SIMS data.
• •TOF-SIMS allows to simultaneously detect proteins and metabolites in clinical samples.

     

Proteaphagy in Mammalian Cells Can Function Independent of ATG5/ATG7

1. Download : Download high-res image (123KB)
2. Download : Download full-size image

Highlights

• •Quantitative proteomics of isolated lysosomes, autophagosomes and proteasomes.
• •Pharmacological inhibition of proteasomes leads to their accumulation within lysosomes.
• •Inhibition of classical autophagy pathways cannot completely block this process.
• •Known autophagy adaptor proteins are not involved.

     

Proteomic Analysis of Salmonella-modified Membranes Reveals Adaptations to Macrophage Hosts

1. Download : Download high-res image (255KB)
2. Download : Download full-size image

Highlights

• •Affinity-based proteomics of infected macrophage cells.
• •Salmonella-modified membranes exhibit host-specific composition.
• •Proteome differences explain some host-dependent pathophysiological differences.