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1.
Activity in vivo and redox States in vitro of nitro- and chlorodiphenyl ether herbicide analogs 总被引:3,自引:3,他引:0
Excised cucumber (Cucumis sativus L. cv 447 Wisconsin SMR 18) cotyledons were sensitive to acifluorfen-methyl (methyl 5-[2-chloro-4-(trifluoromethyl)phenoxy]-2-nitrobenzoate) and MC-15608 (methyl 5-[2-chloro-4-(trifluoromethyl)phenoxy]-2-chlorobenzoate). Injury was detected by monitoring efflux of 3-O-methyl-d-[U-14C]glucose from herbicide-treated tissue after exposure to light. Efflux kinetics of 3-O-methyl-[14C]glucose from cotyledons treated with either acifluorfen-methyl (AFM) or MC-15608 were similar. Neither herbicide was active in darkness. 相似文献
2.
First internode growth of green Vigna sinensis L. can be widely modified by light or dark treatments. In all the treatments used there is a good correlation between the internode growth and the rate of C18-1 accumulation. None of the other fatty acids show such a correlation.Abbreviations C16-0
palmitoic acid
- C17
heptadecanoic acid
- C18-0
stearic acid
- C18-1
octadecenoic acid
- C18-2
linoleic acid
- C18-3
linolenic acid
- D
darkness
- DW
dry weight
- FR
far-red light
- FW
fresh weight
- PFR
phytochrome in the FR absorbing form
- R
red light
- W
white light 相似文献
3.
Farida Begum Noor Barak Almandil Muhammad Arif Lodhi Khalid Mohammed Khan Abdul Hameed Shahnaz Perveen 《Bioorganic & medicinal chemistry》2019,27(6):1009-1022
This study deals with the synthesis of benzophenone sulfonamides hybrids (1–31) and screening against urease enzyme in vitro. Studies showed that several synthetic compounds were found to have good urease enzyme inhibitory activity. Compounds 1 (N′-((4′-hydroxyphenyl)(phenyl)methylene)-4′′-nitrobenzenesulfonohydrazide), 2 (N′-((4′-hydroxyphenyl)(phenyl)methylene)-3′′-nitrobenzenesulfonohydrazide), 3 (N′-((4′-hydroxyphenyl)(phenyl)methylene)-4′′-methoxybenzenesulfonohydrazide), 4 (3′′,5′′-dichloro-2′′-hydroxy-N′-((4′-hydroxyphenyl)(phenyl)methylene)benzenesulfonohydrazide), 6 (2′′,4′′-dichloro-N′-((4′-hydroxyphenyl)(phenyl)methylene)benzenesulfonohydrazide), 8 (5-(dimethylamino)-N′-((4-hydroxyphenyl)(phenyl)methylene)naphthalene-1-sulfono hydrazide), 10 (2′′-chloro-N′-((4′-hydroxyphenyl)(phenyl)methylene)benzenesulfonohydrazide), 12 (N′-((4′-hydroxyphenyl)(phenyl)methylene)benzenesulfonohydrazide) have found to be potently active having an IC50 value in the range of 3.90–17.99?µM. These compounds showed superior activity than standard acetohydroxamic acid (IC50?=?29.20?±?1.01?µM). Moreover, in silico studies on most active compounds were also performed to understand the binding interaction of most active compounds with active sites of urease enzyme. Structures of all the synthetic compounds were elucidated by 1H NMR, 13C NMR, EI-MS and FAB-MS spectroscopic techniques. 相似文献
4.
Walter Heiligenberg Clifford H. Keller Walter Metzner Masashi Kawasaki 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1991,169(2):151-164
Summary The complex of the diencephalic nucleus electrosensorius (nE) provides an interface between the electrosensory processing performed by the torus semicircularis and the control of specific behavioral responses. The rostral portion of the nE comprises two subdivisions that differ in the response properties and projection patterns of their neurons. First, the nEb (Fig. 1 B), which contains neurons that are driven almost exclusively by beat patterns generated by the interference of electric organ discharges (EODs) of similar frequencies. Second, the area medial to the nEb, comprising the lateral pretectum (PT) and the nE-acusticolateralis region (nEar, Fig. 1 B-D), which contains neurons excited predominantly by EOD interruptions, signals associated with aggression and courtship. Neurons in the second area commonly receive convergent inputs originating from ampullary and tuberous electroreceptors, which respond to the low-frequency and high-frequency components of EOD interruptions, respectively. Projections of these neurons to hypothalamic areas linked to the pituitary may mediate modulations of a fish's endocrine state that are caused by exposure to EOD interruptions of its mate.Abbreviations
a
axon
-
ATh
anterior thalamic nucleus
-
CCb
corpus cerebelli
-
CE
central nucleus of the inferior lobe
-
CP
central posterior thalamic nucleus
-
Df
frequency difference between neighbor's EOD and fish's own
-
DFl
nucleus diffusus lateralis of the inferior lobe
-
DFm
nucleus diffusus medialis of the inferior lobe
-
DTn
dorsal tegmental nucleus
-
EOD
electric organ discharge
-
G
glomerular nucleus
-
Hc
caudal hypothalamus
-
Hd
dorsal hypothalamus
-
Hl
lateral hypothalamus
-
Hv
ventral hypothalamus
-
JAR
jamming avoidance response
-
LL
lateral lemniscus
-
MGT
magnocellular tegmental nucleus
-
MLF
medial longitudinal fasciculus
-
nB
nucleus at the base of the optic tract
-
nE
nucleus electrosensorius
-
nEar
nucleus electrosensorius-acusticolateral region
-
nEb
nucleus electrosensorius-beat related area
-
nE
nucleus electrosensorius, area causing rise of EOD frequency
-
nE
nucleus electrosensorius, area causing fall of EOD frequency
-
nLT
nucleus tuberis lateralis
-
nLV
nucleus lateralis valvulae
-
PC
posterior commissure
-
Pd
nucleus praeeminentialis, pars dorsalis
-
PeG
periglomerular complex
-
PG
preglomerular nucleus
-
PLm
medial division of the perilemniscal nucleus
-
Pn
pacemaker nucleus
-
PPn
prepacemaker nucleus
-
PT
pretectal nucleus
-
PTh
prethalamic nucleus
-
R
red nucleus
-
Sc
suprachiasmatic nucleus
-
SE
nucleus subelectrosensorius
-
TAd
nucleus tuberis anterior-dorsal subdivision
-
TAv
nucleus tuberis anterior-ventral subdivision
-
TeO
optic tectum
-
TL
torus longitudinalis
-
TSd
dorsal (electrosensory) torus semicircularis
-
TSv
ventral (mechanosensory and auditory) torus semicircularis
-
tTB
tecto-bulbar tract
-
VCb
cerebellar valvula
-
VP
valvular peduncle
-
VPn
nucleus of the valvular peduncle 相似文献
5.
Yasuhiko Fujino Masao Ohnishi Seisuke Ito 《Bioscience, biotechnology, and biochemistry》2013,77(9):2753-2762
Ceramide and mono-, di-, tri-, and tetraglycosylceramide were isolated from the bran and endosperm of rice grains and chemically characterized. The detailed compositions of free ceramide were somewhat different between the bran and endosperm, but those of the ceramide moiety in glycosylceramides were substantially the same. There was a tendency in all the sphingolipid molecules in rice grains for hydroxy acids with C20 to be combined largely with the dihydroxy bases while hydroxy acids with C24< combined mainly with the trihydroxy bases. Representative molecular species of the sphingolipid classes were concluded to be as follows: for ceramide N-2′-hydroxylignoceroyl-4-hydroxysphinganine, for monoglycosylceramide l-O-β-glucosyl-N-2′-hydroxyarachidoyl-4,8-sphingadienine, for diglycosylceramide 1-O-[β-mannosyl(1→-4)-O-β-glucosyl]- and 1-O-[β-glucosyl(1→4)-O-β-glucosyl]-N-2′-hydroxylignoceroyl-4-hydroxy-8-sphingenine, for triglycosylceramide l-O-[β-mannosyl(1→4)-O-β-mannosyl(l→4)-O-β-glucosyl]- and l-O-[β-glucosyl(l→4)-O-β-mannosyl(1→4)-O-β-glucosyl]-N-2′-hydroxylignoceroyl-4-hydroxy-8-sphingenine, and for tetraglycosylceramide 1-0-[β-mannosyl(l→4)-O-β-mannosyl (1→4)-O-β-mannosyl(1→4)-O-β-glucosyl]- and l-O-[β-glucosyl(1→4)-O-β-mannosyl(l→4)-O-β-mannosyl(1β4)-O-β-glucosyl]-N-2′-hydroxylignoceroyl-4-hydroxy-8-sphingenine. 相似文献
6.
Regulation of the Partitioning of Dry Matter and Calcium in Cucumber in Relation to Fruit Growth and Salinity 总被引:1,自引:0,他引:1
The regulation of the partitioning of dry matter and calciumin relation to fruit growth was investigated in cucumber plantsgrown in the salinity range of 3-8 mS cm-1 in NFT (NutrientFilm Culture), with or without a fruit pruning treatment. Thedry weight gain of the plants was proportional to the outdoorintegral irradiance, with a common daily rate of 1 g MJ-1 m-2in two crops grown under summer (18 MJ m-2 d-1) and autumn (7MJ m-2 d-1) conditions. Within the salinity range studied, thereduction of plant dry weight was 9% mS-1 cm-1. However, fruitdry weight was only reduced at salinities above 5·5 mScm-1, although the daily dry matter accumulation by fruit, asa percentage of total dry matter accumulation, was increased.Salinity reduced the dry matter accumulation in the young shootproportionally more than in the fruit. Although the total plantCa content was reduced by 13% mS-1 cm-1, the Ca content of theyoung shoot was reduced by 16·6%, compared to 11% inthe fruit. Pruning fruit reduced neither plant dry weight norCa uptake. The growth of the remaining fruit, and to a lesserdegree of the young shoot, accounted for all surplus assimilates.Thus, fruit were the dominant sinks for assimilates whilst themature leaves were the strongest sinks for Ca. Nevertheless,the fruit sustained the capacity to import Ca better than theyoung shoot, when supplies of both assimilates and Ca were reducedby high salinity.Copyright 1994, 1999 Academic Press Cucumber, Cucumis sativus L., salinity, fruit pruning, dry matter and calcium 相似文献
7.
G. Purschke 《Zoomorphology》1985,105(4):223-239
Summary A comparative anatomical and ultrastructural study of ventral pharyngeal organs (pharyngeal bulbs) was carried out in two species of the Dinophilidae: Dinophilus gyrociliatus and Trilobodrilus axi. Special attention was paid to the fine structure of the stomodeal epithelium, cuticle, glands, muscles, and myoepithelial junctions. The differences between the species are very slight. The pharyngeal organ of the Dinophilidae is characterized by the following features: solid muscle bulbus made up of muscle cells only, bulbus muscle cells with two myofilament systems crossing at an angle of about 90°, gap junctions between these muscle cells, bulbus projects into a pharyngeal sac and bears rostrally a specific epithelium and cuticle, no bulbus glands, no investing (= sagittal) muscles, specific cuticle ultrastructure, cilia of ascending oesophagus with asymmetric tips, specific structure and position of salivary gland openings. The phylogenetic importance of these structures is discussed. Some of these characters are clearly autapomorphic features of the Dinophilidae and no common derived structures to other families with a ventral pharyngeal organ are present. Therefore, it is most likely that the dinophilid pharyngeal organ evolved independently. These findings do not agree with the hypothesis of the unity of the archiannelid families (Polygordiidae, Protodrilidae, Saccocirridae, Nerillidae, Dinophilidae, and Diurodrilidae) established on the basis of an assumed structural similarity of their ventral pharyngeal organs.Abbreviations
bb
basal body
-
bep
bulbus epithelium
-
bl
basal lamina
-
bm
bulbus muscle
-
c
cilium
-
cc
coelenchyme cell
-
cm
circular muscle
-
cr
caudal rootlet
-
cu
cuticle
-
dblm
dorsal bulbus longitudinal muscle
-
dlm
dorsal longitudinal muscle
-
dsn
dorsal stomatogastric nerve
-
dy
dyad
-
el
electron-dense layer
-
fl
fibrous layer
-
fi
filaments
-
g
Golgi apparatus
-
gl
gland cell
-
hv
homogeneous vesicle
-
l
lipid droplet
-
la
external lamina
-
lal
lamellar layer
-
ll
lower lip
-
lm
longitudinal muscle
-
ly
lysosome
-
m
mitochondrion
-
mo
mouth opening
-
mt
microtubule
-
mv
microvillus
-
mvp
microvillar process
-
n
nucleus
-
nu
nucleolus
-
oes
oesophagus
-
pcom
preoral commissure
-
phf
pharyngeal fold
-
phl
pharyngeal lumen
-
phs
pharyngeal sac
-
pms
peripheral myofilament system
-
r
rootletlike structure
-
rer
rough endoplasmic reticulum
-
rr
rostral rootlet
-
s
sarcoplasmic reticulum
-
sc
salivary canal
-
scom
suboesophageal commissure
-
sd
septate desmosome
-
ser
smooth endoplasmic reticulum
-
sg
secretory granule
-
sgl
salivary gland
-
sn
stomatogastric nerve
-
st
stomach
-
step
stomodeal epithelium
-
tep
transitional epithelium
-
tf
tonofilaments
-
va
vacuole
-
vlm
ventral longitudinal muscle
-
vsn
ventral stomatogastric nerve
-
z
z-element
-
za
zonula adherens 相似文献
8.
Fine structure and formation of eggshells in marine Gastrotricha 总被引:1,自引:2,他引:1
Summary The fine structure of the gastrotrich eggshell in the hermaphroditic species Turbanella ocellata (Hummon 1974) and the parthenogenetic species Aspidiophorus sp. is described using transmission electron microscopy. The presented evidence strongly suggests that the shell is produced by the egg itself prior to oviposition in both species. The layed egg in Aspidiophorus sp. is provided with a special attachment stalk that is also preformed in the mother animal. Freshly layed eggs of T. ocellata are adhesive all around their surface and lack any specialized structures for attachment. Formation of the spiny eggshell of Aspidiophorus sp. appears to begin with a sudden release of special vesicles containing the preformed spines of the outer eggshell covering. Additional material appears to be secreted by the egg in a more gradual process after the initial vesicle release. The formation of the two fibrous layers in the eggshell of T. ocellata is less well understood and deposition of eggshell material could be seen either as a continuous process or as two separate steps, similar to the events observed for Aspidiophorus sp. For T. ocellata, Tetranchyroderma sp. and Aspidiophorus sp. it is demonstrated that formation of the cuticle occurs as an independent process from that of eggshell formation. This is significantly different from the basic mode of cuticle formation in the annelid line of evolution. The paper argues further that the data support earlier claims of a pronounced difference between the Gastrotricha-Macrodasyida and the Gastrotricha-Paucitubulatina and agree well with the postulated ties of the Gastrotricha and Nematoda. The phylogenetic importance of the eggshell fine-structure is discussed in the framework of present theories on aschelminth phylogeny.Abbreviations cus
cuticular spines
- cut
cuticle
- cov
coated vesicles
- cv
cup-shaped vesicles
- dp
dense particles
- ep
epidermis
- emb
embryo
- erl
lacunae of smooth ER
- fgb
fibrous and granular bodies
- fl
fibrous layer
- ga
Golgi apparatus
- gc
gut cell
- gv
Golgi vesicles
- im
intercellular matrix
- isp
intercellular space
- isl
inner shell layer
- ld
lipid droplet
- mdb
medium-dense bodies
- mvb
multivesicular bodies
- oc
oocyte
- od
oviduct
- osl
outer shell layer
- o
egg
- sv
spiny vesicles
- sh
eggshell
- st
egg-stalk
- sl
spiny layer
- sub
substrate
- trm
trilaminate membrane
- yb
yolk bodies
- yg
yolk granule
- yoc
young oocyte
This work was supported by NSF Grant # GB-42211 to R.M. Rieger 相似文献
9.
The problem of optimising agitation and aeration in a given fermenter is addressed. The objective function is total electric power consumed for agitation, compression and refrigeration. The major constraint considered is to ensure that the dissolved oxygen concentration is above the critical value. It is shown that it is possible to analytically calculate the optimal pair (air flowrate, stirrer speed) and that, at least for the industrial antibiotics fermentation used as case-study, the optimum lies within a window for satisfactory operation, limited by other possible constraints to the problem. Savings achievable by optimal operation as compared with current industrial procedure were found to be around 10% at pilot plant scale (0.26 m3) and 20% at full scale (85 m3).List of Symbols
A
fermenter cross sectional area (m2)
-
C
dissolved oxygen concentration (mole m–3)
-
C
*
DO concentration in equilibrium with the gas (mole m–3)
-
C
crit
critical DO concentration (mole m–3)
-
C
p
specific heat of air at constant pressure (J kg–1 K–1)
-
C
sp
dissolved oxygen set point (mole m–3)
-
C
v
specific heat of air at constant volume (J kg–1 K–1)
-
D
agitator diameter (m)
-
f
pressure correction of air flow-rate
-
(Fl
g)F
aeration number at flooding
-
(Fr
g)F
froude number at flooding
-
k
coefficient in expression for mass transfer coefficient
-
K
La
volumetric oxygen transfer coefficient (s–1)
-
m
power exponent in expression for mass transfer coefficient
-
n
gas flow rate exponent in expression for mass transfer coefficient
-
n
*
number of impellers
-
N
rotation speed (s–1)
-
N
F
rotation speed at flooding (s–1)
-
N
p
unaerated power number
-
N
pg
aerated power number
-
OUR
Oxygen Uptake Rate (mole m–3 s–1)
-
p
0
atmospheric pressure (N m–2)
-
p
1
compressor exit pressure (N m–2)
-
p
2
pressure at the bottom of the fermenter (N m–2)
-
p
3
pressure at the top of the fermenter (N m–2)
-
P
c
compression power (W)
-
P
d
power added by expansion (W)
-
P
ev
power removed by evaporation (W)
-
P
g
agitation power (W)
-
P
m
power added by metabolism (W)
-
P
r
power removed by refrigeration (W)
-
P
t
total power (W)
-
Q
air flow-rate at atmospheric conditions (m3 s–1)
-
Q
f
air flow-rate at average fermenter conditions (m3 s–1)
-
s
0
absolute humidity at atmospheric conditions
-
s
3
absolute humidity at fermenter exit
-
T
tank diameter (m)
-
V
liquid volume (m3)
-
v
s
gas superficial velocity (m s–1)
-
i
parameter defined in the text
-
safety margin for dissolved oxygen (mole m–3)
-
ratio of specific heats of air
-
g
agitation efficiency
-
c
compression efficiency
-
r
refrigeration efficiency
-
liquid density (kg m–3)
-
g
air density (kg m–3)
-
latent heat of vaporisation of water (J kg–1)
The authors are grateful to Elsa Silva, Carlos Lopes, Carlos Aguiar, Fernando Mendes, and Alexandre Cardoso, who helped with parts of this work, and to CIPAN for permission to publish these data. 相似文献
10.
In this research, investigations were made on material transfer mechanisms and optimum operation mode for sequencing batch reactor system removing phosphorus and nitrogen simultaneously. Phosphorus release characteristics were expressed in the Monod equation, in which the reaction rate was replaced with specific phosphorus release (SPR) rate. The rate of SPR was increased during the first 80 days, but increased sharply to reach 0.003 hr-1 afterwards. Phosphorus removal efficiencies were about 60% in the first 80 days, 75% after 80 days, and above 95% after 120 days. After 120 days, phosphorus concentration in effluent was below 0.5 mgl-1 when 8 mgl-1 was in the influent and the released phosphorus after 3-hour-anaerobic period was 60 mgl-1. In the proposed optimum operation strategy (2-hour anaerobic react, 3-hour aerobic react, 4-hour anoxic react, and 3-hour settle and draw), phosphorus reappeared if the oxidized nitrogen was completely denitrified. In order to prevent this undesirable phosphorus release, anoxic period should be reduced to the extent of which the minimal concentration of the oxidized nitrogen existed. Phosphorus removal efficiency was stable under shock load as 5 times high as normal phosphorus concentration.Abbreviations dP/dt
Phosphorus release rate (mgl-1 hr-1)
- K
Phosphorus release yield constant (mg P mg TOC-1)
- dS/dt
Substrate utilization rate (mgl-1 hr-1)
- X
Mixed liquor suspended solid (MLSS, mgl-1)
- S
Soluble TOC (mgl-1)
- k-qmax (Ymax)-1
Maximum substrate utilization rate
- Y
Yield coefficient (mg mg-1)
- Ks
Saturation constant (mgl-1)
- Pmax
kK-Maximum phosphorus release rate (hr-1)
- Prel
Total released phosphorus (mgl-1)
- Po
Phosphorus in influent (mgl-1)
- Pe
phosphorus in effluent (mgl-1)
- t
Anaerobic period (hr) 相似文献
11.
M. Essig S. Ledoux P.-L. Tharaux C. Chatziantoniou J.-C. Dussaule 《Cell biology and toxicology》1996,12(4-6):271-274
Various in vitro preparations have been utilized to study the cellular activity of vasoactive agents on renal cortical microvessels and on mesangial cells. The receptors and the transduction pathways of bradykinin and atrial natriuretic factor were characterized on cultured cortical vascular smooth muscle cells from the rabbit kidney. A preparation of afferent arterioles that had been freshly isolated from the rat kidney was used to study the NO-dependent regulation of renin release. The influence of endothelin and angiotensin II on mesangial cell proliferation was evaluated, using cocultures of human endothelial and mesangial cells. These appropriate in vitro preparations have provided new insights on renal vascular endocrinology. However, extrapolation of in vitro data to in vivo physiology must be cautious because the phenotype of vascular cells often changes in culture conditions.Abbreviations ANF
atrial natriuretic factor
- BK
bradykinin
- CNP
C-type natriuretic peptide
- ET-1
endothelin-1
- HUVEC
human umbilical vein endothelial cells
- IBMX
isobutylmethylxanthine
- NEP
neutral endopeptidase
- PKA
protein kinase A
- RCVSMC
renal cortical vascular smoothmuscle cells 相似文献
12.
Phytoplankton composition, density, vertical distribution andprimary production were investigated in the Sea of Okhotsk andin the adjacent northern north Pacific in JulyAugust1994, together with measurements of density and distributionof planktonic microheterotrophs: bacteria, nanoheterotrophsand ciliates. Different phases of phytoplankton seasonal successionwere encountered during the period of investigation in variousregions of this sea. Primary production measured at 144 stationswas found to be greatest (1.54 g C m-2day-1) in areasof spring-phase succession along the Sakhalin shelf and theKashevarov bank. Periodic relapses of the spring blooms of heavydiatoms during the whole growth season were recorded over thisbank. The summer phase of the phytoplankton minimum prevailedin the central and eastern parts of the sea, manifested by thedominance of nanoflagellates in terms of phytoplankton biomass.Primary production was 0.51 g C m-2 day-1. The earlyautumn phase of succession was typical of the Kurile straitarea and the adjacent north Pacific. Primary production therevaried from 0.7 to 2 g C m-2 day-1. The integrated phytoplanktonbiomass in the water column varied from 912 g m-2 inzones supporting the summer minimum assemblage to 1520g m-2 in zones of early autumn recovery of phytoplankton growth,and up to 4070 g m-2 in areas of remnant or relapseddiatom blooms. The numerical density of bacterioplankton wasbetween 1 x 106 and 3 x 106 cells ml-1 and its wet biomass wasbetween 100 and 370 mg m-3. In deep waters it was 815mg m-3. The integrated bacterioplankton biomass in the upperwater column varied from 6 to 29 g m-2. The numerical densityof zooflagellates varied in the upper layer between 0.8 x 106and 4 x 106 l-1 and their biomass was between 20 and 50 mg m-3.In deep waters they were still present at a density of 0.05x 106 to 0.2 x 106 cells l-1. The biomass of planktonic ciliatesvaried between stations from 20 to 100 mg m-3. The joint biomassof planktonic protozoa in the water column was between 3 and12 g m-3 at most of the stations. 相似文献
13.
N. Aste C. Viglietti-Panzica A. Fasolo C. Andreone H. Vaudry G. Pelletier G. C. Panzica 《Cell and tissue research》1991,265(2):219-230
Summary In the present study, we have demonstrated, by means of the biotin-avidin method, the widespread distribution of neuropeptide Y (NPY)-immunoreactive structures throughout the whole brain of the Japanese quail (Coturnix coturnix japonica). The prosencephalic region contained the highest concentration of both NPY-containing fibres and perikarya. Immunoreactive fibres were observed throughout, particularly within the paraolfactory lobe, the lateral septum, the nucleus taeniae, the preoptic area, the periventricular hypothalamic regions, the tuberal complex, and the ventrolateral thalamus. NPY-immunoreactive cells were represented by: a) small scattered perikarya in the telencephalic portion (i.e. archistriatal, neostriatal and hyperstriatal regions, hippocampus, piriform cortex); b) medium-sized cell bodies located around the nucleus rotundus, ventrolateral, and lateral anterior thalamic nuclei; c) small clustered cells within the periventricular and medial preoptic nuclei. The brainstem showed a less diffuse innervation, although a dense network of immunopositive fibres was observed within the optic tectum, the periaqueductal region, and the Edinger-Westphal, linearis caudalis and raphes nuclei. Two populations of large NPY-containing perikarya were detected: one located in the isthmic region, the other at the boundaries of the pons with the medulla. The wide distribution of NPY-immunoreactive structures within regions that have been demonstrated to play a role in the control of vegetative, endocrine and sensory activities suggests that, in birds, this neuropeptide is involved in the regulation of several aspects of cerebral functions.Abbreviations
AA
archistriatum anterius
-
AC
nucleus accumbens
-
AM
nucleus anterior medialis
-
APP
avian pancreatic polypeptide
-
CNS
centrai nervous system
-
CO
chiasma opticum
-
CP
commissura posterior
-
CPi
cortex piriformis
-
DIC
differential interferential contrast
-
DLAl
nucleus dorsolateralis anterior thalami, pars lateralis
-
DLAm
nucleus dorsolateralis anterior thalami, pars medialis
-
E
ectostriatum
-
EW
nucleus of Edinger-Westphal
-
FLM
fasciculus longitudinalis medialis
-
GCt
substantia grisea centralis
-
GLv
nucleus geniculatus lateralis, pars ventralis
-
HA
hyperstriatum accessorium
-
Hp
hippocampus
-
HPLC
high performance liquid chromatography
-
HV
hyperstriatum ventrale
-
IF
nucleus infundibularis
-
IO
nucleus isthmo-opticus
-
IP
nucleus interpeduncularis
-
IR
immunoreactive
-
LA
nucleus lateralis anterior thalami
-
LC
nucleus linearis caudalis
-
LFS
lamina frontalis superior
-
LH
lamina hyperstriatica
-
LHRH
luteinizing hormone-releasing hormone
-
LoC
locus coeruleus
-
LPO
lobus paraolfactorius
-
ME
eminentia mediana
-
N
neostriatum
-
NC
neostriatum caudale
-
NPY
neuropeptide Y
-
NIII
nervus oculomotorius
-
NV
nervus trigeminus
-
NVI
nervus facialis
-
NVIIIc
nervus octavus, pars cochlearis
-
nIV
nucleus nervi oculomotorii
-
nIX
nucleus nervi glossopharyngei
-
nBOR
nucleus opticus basalis (ectomamilaris)
-
nCPa
nucleus commissurae pallii
-
nST
nucleus striae terminalis
-
OM
tractus occipitomesencephalicus
-
OS
nucleus olivaris superior
-
PA
palaeostriatum augmentatum
-
PBS
phosphate-buffered saline
-
POA
nucleus praeopticus anterior
-
POM
nucleus praeopticus medialis
-
POP
nucleus praeopticus periventricularis
-
PP
pancreatic polypeptide
-
PYY
polypeptide YY
-
PVN
nucleus paraventricularis magnocellularis
-
PVO
organum paraventriculare
-
R
nucleus raphes
-
ROT
nucleus rotundus
-
RP
nucleus reticularis pontis caudalis
-
Rpc
nucleus reticularis parvocellularis
-
RPgc
nucleus reticularis pontis caudalis, pars gigantocellularis
-
RPO
nucleus reticularis pontis oralis
-
SCd
nucleus subcoeruleus dorsalis
-
SCv
nucleus subcoeruleus ventralis
-
SCNm
nucleus suprachiasmaticus, pars medialis
-
SCNl
nucleus suprachiasmaticus, pars lateralis
-
SL
nucleus septalis lateralis
-
SM
nucleus septalis medialis
-
Ta
nucleus tangentialis
-
TeO
tectum opticum
-
Tn
nucleus taeniae
-
TPc
nucleus tegmenti pedunculo-pontinus, pars compacta
-
TSM
tractus septo-mesencephalicus
-
TV
nueleus tegmenti ventralis
-
VeL
nucleus vestibularis lateralis
-
VLT
nucleus ventrolateralis thalami
-
VMN
nucleus ventromedialis hypothalami
A preliminary report of this study was presented at the 15th Conference of European Comparative Endocrinologists, Leuven, Belgium, September 1990 相似文献
14.
《Journal of enzyme inhibition and medicinal chemistry》2013,28(6):1267-1273
Some novel derivatives of Bis-chalcone were synthesized and characterized by their physical and spectral data. All the synthesized compounds were subsequently screened for in vitro globin hydrolysis, β-hematin formation, and murine Plasmodium berghei, using chloroquine as the reference drug. Most of the synthesized compounds exhibited mild to moderate susceptibilities toward the parasite in comparison with the standard. The most active antimalarial compound was 1,1-Bis-[(3′,4′-N-(urenylphenyl)-3-(3″,4″,5″-trimethoxyphenyl)]-2-propen-1-one 5, with a percentage of inhibition of heme polymerization of 87.05?±?0.77, and this compound increased the survival time after infection, reduce the parasitemia and delay the progression of malaria. 相似文献
15.
Changes in expression of provasotocin and proisotocin genes during adaptation to hyper- and hypo-osmotic environments in rainbow trout 总被引:1,自引:0,他引:1
Susumu Hyodo Akihisa Urano 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1991,161(6):549-556
Summary The physiological roles of neurohypophysial hormones, vasotocin (VT) and isotocin (IT), are not yet clear in teleosts. Since information on responsiveness of hypothalamic neurosecretory neurons to environmental stimuli may contribute to an understanding of their physiological roles, effects of environmental hyper- and hypo-osmotic stimuli on expression of VT and IT precursor (proVT and proIT) genes in rainbow trout were investigated, using an in situ hybridization technique in which 46 mer synthetic oligonucleotides were used as hybridization probes. The probes corresponded to the mRNA loci encoding chum salmon proVT (-5 to 11) and proIT (-5 to 11), and were labeled at the 3-end with 35S. Autoradiographic silver grains which represent the hybridization signals of proVT and proIT mRNAs were localized in both magnocellular and parvocellular neurons in the nucleus preopticus magnocellularis (NPOmg). Localizations of proVT and proIT hybridization signals coincided with those of VT- and IT-immunoreactive neurons in adjacent sections, and showed that proVT and proIT genes are expressed in separate neurons. The intensity of proVT hybridization signals as determined by grain counting in magnocellular neurons in the NPOmg was conspicuously decreased after transfer from fresh water (FW) to 80% seawater (SW). The proVT mRNA levels in SW trout were consistently lower than those of FW trout for up to 2 weeks. After return from 80% SW to FW, the proVT mRNA level increased, attaining the initial FW level. The proIT mRNA levels in SW trout were not statistically different from those in FW trout, except for the 1st day after transfer to SW. These results suggest that synthesis of proVT was elevated by transfer from higher to lower salinity, and that VT may have a physiological role in salmonid osmoregulation, especially in adaptation to a hypo-osmotic environment.Abbreviations
ABC
avidin-biotin-peroxidase complex
-
AVP
arginine vasopressin
-
BSA
bovine serum albumin
-
EDTA
ethylene diamine tetra-acetic acid
-
cpm
counts per minute
-
FW
fresh water
-
GFR
gromerular filtration rate
-
ISH
in situ hybridization
-
IT
isotocin
-
mRNA
messenger ribonucleic acid
-
NPOmg
nucleus preopticus magnocellularis
-
OXT
oxytocin
-
PBS
phosphate-buffered saline
-
SSC
saline sodium citrate
-
RT
room temperature
-
SW
seawater
-
VT
vasotocin 相似文献
16.
The effects of FSH, LH, and epidermal growth factor (EGF) on the dynamics of nuclear maturation and subsequent embryo development were examined in pig oocytes cultured either conventionally or after preincubation with cycloheximide (CHX). In conventional culture, FSH or EGF significantly increased the rate of attainment of metaphase II (MII) for both gilt (50.0%+/-4.2% and 54.8%+/-4.3%, respectively; control, 5.8%+/-1.8%; P<0.001) and sow (87.6%+/-3.4% and 78.8%+/-3.9%, respectively; control, 7.8%+/-2.5%; P<0.001) oocytes. Gilt oocytes treated with both FSH and EGF showed an additive response (93.7%+/-2.1%). Treatment with LH had no effect. Preincubation with CHX caused the majority (84-100%) of both gilt and sow oocytes to undergo germinal vesicle breakdown. Compared to those treated with LH and/or EGF (both>80%), fewer FSH-treated oocytes reached metaphase I (43.8%+/-5.3%, P<0.001) by 14 h and MII (48.4%+/-5.9%, P<0.001) by 24 h, although the majority (71%) did mature to MII by 36 h after removal of CHX. After in vitro fertilization, higher proportions of both CHX-pretreated and untreated, FSH-exposed oocytes cleaved (71.3%+/-2.9% and 75.3%+/-3.1%, respectively) compared with those not treated with FSH (37.7%+/-3.0% and 43.0%+/-2.9%, respectively; P<0.001). Pretreatment with CHX significantly increased blastocyst yield for both FSH-treated (32.8%+/-2.0% and 10.3%+/-1.5%, respectively; P<0.001) and untreated (16.7%+/-1.5% and 9.4%+/-1.2%, respectively; P<0.001) oocytes. Polyspermy rates were unaffected. In conclusion, pig oocytes meiotically arrested by CHX before maturation retain and improve their developmental competence. FSH stimulates nuclear maturation but slows meiotic progression. 相似文献
17.
Th. Thepen P. Voorn Dr. C. J. Stoll A. A. Sluiter C. W. Pool A. H. M. Lohman 《Cell and tissue research》1987,250(3):649-656
Summary The distribution of mesotocin and vasotocin was studied in the brain of the lizard Gekko gecko with antisera specific for either peptide. Both mesotocinergic and vasotocinergic perikarya are found in the paraventricular and supraoptic nuclei of the hypothalamus, whereas vasotocinergic neurons are exclusively present in the bed nucleus of the stria terminalis and in a cell group of the rhombencephalon. The distributional pattern of the mesotocinergic fibers corresponds closely to that of the vasotocinergic fibers. However, throughout the entire brain the mesotocinergic innervation is less dense than the vasotocinergic innervation. No sex differences are present in the mesotocinergic fiber system.Abbreviations
acc
nucleus accumbens
-
bst
bed nucleus of the stria terminalis
-
bv
blood vessel
-
dB
diagonal band of Broca
-
dc
dorsal cortex
-
dth
dorsolateral thalamic nucleus
-
lc
lateral cortex
-
me
median eminence
-
oc
optic chiasma
-
ot
optic tract
-
pag
periaqueductal grey
-
pvn
paraventricular nucleus
-
rc
rhombencephalic cell group
-
sep
septum
-
son
supraoptic nucleus
-
tect
mesencephalic tectum
-
vth
ventrolateral thalamus 相似文献
18.
The binding characteristics and the inhibitory power of atrazine and DCMU towards uncoupled electron flow activity were studied in acyl lipid-depleted thylakoid membranes from atrazine-susceptible and-resistant biotypes of Solanum nigrum L. For this purpose, phospholipase A2 from Vipera russelli and the lipase from Rhizopus arrhizus were used to obtain a selective lipid class (phospholipids or galactolipids) depletion which was restricted to the outer monolayer. Neither phospholipid nor galactolipid removal affected the dissociation constant and the number of binding sites of atrazine. In contrast, the dissociation constant of DCMU was increased in phospholipid-depleted thylakoid membranes but remained unchanged after galactolipid depletion. The number of DCMU binding sites decreased significantly after both lipase treatments, but only in the resistant biotype. The inhibitory effectiveness of the herbicide was either decreased or increased (to different extents) depending on the lipid class which was removed from the membrane and on the biotype considered. These results are discussed with reference to the possible conformational changes of the 32 kDa herbicide-binding polypeptide occurring after lipase treatments.Abbreviations Atrazine
2-chloro-4-(ethylamino)-6-(isopropylamino)-s-triazine
- BSA
bovine serum albumin
- DCMU
diuron, 3-(3,4-dichlorophenyl)-1,1-dimethylurea
- DGDG
digalactosyldiacylglycerol
- LRa
lipase from Rhizopus arrhizus
- MGDG
monogalactosyldiacylglycerol
- PC
phosphatidylcholine
- PG
phosphatidylglycerol
- PLA2
phospholipase A2
- R
atrazine-resistant
- S
atrazinesusceptible 相似文献
19.
(?)-Ephedrine has been shown to increase energy expenditure and cause the loss of body fat in rats and mice. The present study compares the effects of (?)- and (+)-ephedrine, (?)- and (+)- pseudoephedrine, (±)- and (+)-norephedrine and (?)- and (+)-norpseudoephedrine on food intake, energy expenditure and body composition in / and normal mice and food intake in rats. The most potent anorectic and thermogenic compounds had the S configuration at the C-2 position but the orders of potencies for the compounds for anorexia and thermogenesis were not identical. The potencies of the compounds in reducing body lipid content correlated better with their thermogenic than their anorectic potencies. 相似文献
20.
Summary Mapping of monoaminergic systems in the brain of the newt Triturus alpestris was achieved with antisera against (1) thyrosine hydroxylase (TH), (2) formaldehyde-conjugated dopamine (DA), and (3) formaldehyde-conjugated serotonin (5-HT). In the telencephalon, the striatum was densely innervated by a large number of 5-HT-, DA-and TH-immunoreactive (IR) fibers; IR fibers were more scattered in the amygdala, the medial and lateral forebrain bundles, and the anterior commissure. In the anterior and medial diencephalon, TH-IR perikarya contacting the cerebrospinal fluid (CSF-C perikarya) were located in the preoptic recess organ (PRO), the organum vasculosum laminae terminalis and the suprachiasmatic nucleus. Numerous TH-IR perikarya, not contacting the CSF, were present in the posterior preoptic nucleus and the ventral thalamus. At this level, DA-IR CSF-C neurons were only located in the PRO. In the posterior diencephalon, large populations of 5-HT-IR and DA-IR CSF-C perikarya were found in the paraventricular organ (PVO) and the nucleus infundibularis dorsalis (NID); the dorsal part of the NID additionally presented TH-IR CSF-C perikarya. Most regions of the diencephalon showed an intense monoaminergic innervation. In addition, numerous TH-IR, DA-IR and 5-HT-IR fibers, orginating from the anterior and posterior hypothalamic nuclei, extended ventrally and reached the median eminence and the pars intermedia of the pituitary gland. In the midbrain, TH-IR perikarya were located dorsally in the pretectal area. Ventrally, a large group of TH-IR cell bodies and some weakly stained DA-IR and 5-HT-IR neurons were observed in the posterior tuberculum. No dopaminergic system equivalent to the substantia nigra was revealed. The possible significance of the differences in the distribution of TH-IR and DA-IR neurons is discussed, with special reference to the CSF-C neurons.Abbreviations
AM
amygdala
-
CAnt
commissura anterior
-
CH
commissura hippocampi
-
CP
commissura posterior
-
Ctm
commissura tecti mesencephali
-
DH
dorsal hypothalamus
-
DTh
dorsal thalamus
-
FLM
fasciculus longitudinalis medialis
-
Fsol
fasciculus solitarius
-
H
habenula
-
LFB
lateral forebrain bundle
-
ME
median eminence
-
MFB
medial forebrain bundle
-
NID
nucleus infundibularis dorsalis
-
nIP
neuropil of nucleus interpeduncularis
-
NPOP
nucleus preopticus posterior
-
NS
nucleus septi
-
OVLT
organum vasculosum laminae terminalis
-
PD
pars distalis
-
Pdo
dorsal pallium
-
PHi
primordium hippocampi
-
PI
pars intermedia
-
Pl
lateral pallium
-
PN
pars nervosa
-
PRO
preoptic recess organ
-
Ptec
pretectal area
-
PVO
paraventricular organ
-
Ra
nucleus raphe
-
Rm
nucleus reticularis medius
-
SCO
subcommisural organ
-
ST
striatum; strm stria medullaris thalami
-
strt
stria terminalis thalami
-
TM
tegmentum mesencephali
-
TO
tectum opticum
-
TP
tuberculum posterius
-
trch
tractus cortico-habenularis
-
trmp
tractus mamillopeduncularis
-
VH
ventral hypothalamus
-
Vm
nucleus motorius nervi trigemini
-
VTh
ventral thalamus
-
II
optic nerve 相似文献