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1.
《Molecular & cellular proteomics : MCP》2020,19(6):1005-1016
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- •Brain membrane protein extraction.
- •Protein prenylation.
- •Prenyl peptide capture and characterization by LC-MS/MS.
- •HCD and EThcD peptide fragmentation.
2.
《Molecular & cellular proteomics : MCP》2020,19(5):828-838
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- •Higher AGC significantly improves quantitation quality in single-cell analysis.
- •The boosting-to-sample ratio should be carefully evaluated and optimized.
- •iBASIL allows for precise quantitation of 1,500 proteins from 104 AML single cells.
- •iBASIL recapitulates major biological differences in different AML single cells.
3.
《Molecular & cellular proteomics : MCP》2020,19(3):456-466
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- •Urinary proteomes of patients with recurrent UTI, renal scarring, and VUR.
- •80 proteins differentially expressed, compared to healthy controls.
- •62 proteins may be indicative of susceptibility for UTI.
- •Altered acute phase response, extracellular matrix and carbohydrate metabolism.
4.
《Molecular & cellular proteomics : MCP》2020,19(11):1767-1776
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- •N-glycan patterns are distinct in pediatric and adult urine.
- •Sex differences of N-glycans are much larger in adults.
- •Pediatric urine has almost no sex differences in N-glycan levels.
- •In adults, the majority of N-glycans were more abundant in males.
5.
《Molecular & cellular proteomics : MCP》2020,19(3):444-455
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- •Human spermatozoa possess cells of poor morphology that lack nuclear integrity.
- •These cells can be isolated by density separation.
- •Mass spectrometry reveals their nuclei contain excess protein.
- •TOP2A is a promising marker of this poor nuclear development.
6.
Nataly Mancette Rijensky Netta R. Blondheim Shraga Eilon Barnea Nir Peled Eli Rosenbaum Aron Popovtzer Solomon M. Stemmer Alejandro Livoff Mark Shlapobersky Neta Moskovits Dafna Perry Eitan Rubin Itzhak Haviv Arie Admon 《Molecular & cellular proteomics : MCP》2020,19(8):1360-1374
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- •Sufficient tumor tissues are often unavailable large HLA peptidome discovery.
- •Using patient derived xenograft (PDX) tumors can overcome this limitation.
- •The large PDX HLA peptidomes expand significantly those of the original biopsies.
- •The HLA peptidomes of the PDX tumors included many tumor antigens.
7.
《Molecular & cellular proteomics : MCP》2020,19(11):1910-1920
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- •PRMT5 glutathionylation is increased in aged mice or under oxidative stress.
- •Deglutathionylation of PRMT5 is catalyzed by glutaredoxin-1.
- •PRMT5 glutathionylation decreases its methyltransferase activity.
- •PRMT5 glutathionylation results in G2/M arrest and inhibits cell proliferation.
8.
《Molecular & cellular proteomics : MCP》2020,19(4):589-607
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- •P. aeruginosa grown with exosiderophores and analyzed by proteomic and RT-qPCR.
- •Catechol-type exosiderophores strongly induce the expression of their transporters.
- •Repression of the endogenous iron uptake pathways.
- •Complex phenotypic plasticity in the expression of the various iron-uptake pathways.
9.
《Molecular & cellular proteomics : MCP》2020,19(4):655-671
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- •Acute inhibition of growth-regulatory AGC-kinases Sch9, PKA and Ypk1.
- •Phospho-proteomic profiling of 6373 phsopho-sites.
- •Ypk1 regulates phospho-sites in RRxS/T-context and functionally overlaps with PKA.
- •Ypk1 and PKA activate trehalase activity of Nth1.
10.
《Molecular & cellular proteomics : MCP》2020,19(1):1-10
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- •Co-elution stands out as a global interactome mapping method.
- •Benefits include all-to-all protein analysis and measurement of interactome perturbations.
- •Different separation, quantification and bioinformatic strategies are available.
- •Design considerations depend largely on system under study.
11.
《Molecular & cellular proteomics : MCP》2020,19(7):1120-1131
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- •Quantitative proteomics of isolated lysosomes, autophagosomes and proteasomes.
- •Pharmacological inhibition of proteasomes leads to their accumulation within lysosomes.
- •Inhibition of classical autophagy pathways cannot completely block this process.
- •Known autophagy adaptor proteins are not involved.
12.
《Molecular & cellular proteomics : MCP》2020,19(3):540-553
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- •Repeatable quantification of 200 proteins in dried blood spots.
- •Determined lower limit of quantification, repeatability, parallelism and stability.
- •Protein stability in DBS stored at ambient temperatures for up to 2 months.
- •Concentration ranges for 200 proteins in 20 healthy individuals.
13.
《Molecular & cellular proteomics : MCP》2020,19(1):50-64
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- •BioID reveals the proximity partners of RSK family members.
- •All RSK isoforms associate with and phosphorylate p120ctn on Ser320.
- •RSK negatively regulates adherens junctions and reduces cell-cell adhesion.
- •p120ctn phosphorylation plays a role in the reorganization of proximity partners.
14.
Yi-Han Lin Maryann P. Platt Haiyan Fu Yuan Gui Yanlin Wang Norberto Gonzalez-Juarbe Dong Zhou Yanbao Yu 《Molecular & cellular proteomics : MCP》2020,19(12):2030-2047
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- •Cecal Ligation Puncture (CLP) mouse model to study sepsis-induced kidney disease.
- •Quantitative global proteome and phosphoproteome profiling of mouse kidneys.
- •Highly significant candidate markers for onset and progression of AKI to CKD.
- •Mechanistic insights into sepsis-associated kidney injuries.
15.
《Molecular & cellular proteomics : MCP》2020,19(4):690-700
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- •Two molecular groups in anal squamous carcinoma according proteomic profile.
- •Differences in possible targeted processes such as metabolism or immune response.
- •Different percentage of tumor lymphocyte infiltration.
- •Difference in the frequency of ATM variants, related to PPAR inhibitors.
16.
《Molecular & cellular proteomics : MCP》2020,19(3):478-489
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- •Comprehensive molecular profiling of cutaneous and cerebellar metastasis variants.
- •Identification of differentially regulated metastasis-associated molecules.
- •Evidence for individually distinct patterns of metastasis-associated molecules.
- •Highlighting the evident need for establishing meta-analyses strategies.
17.
《Molecular & cellular proteomics : MCP》2020,19(5):744-756
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- •Signaling networks can be highly heterogeneous across cells in a tissue.
- •Various technologies allow analyzing signaling networks at single-cell resolution.
- •The advantages and limitations of each single-cell approach are summarized.
- •Confounding factors in single-cell signaling network analysis are discussed.
18.
Prashali Bansal Johannes Madlung Kristina Schaaf Boris Macek Fulvia Bono 《Molecular & cellular proteomics : MCP》2020,19(9):1485-1502
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- •Label-free and dimethyl labeling MS analysis of 6 RBPs from Drosophila ovaries.
- •Functionally related RBPs show overlapping proteomes.
- •Selective co-purification of splicing factors and translational regulators.
- •Validation of 26 novel interactions by co-immunoprecipitation.
19.
《Molecular & cellular proteomics : MCP》2020,19(6):928-943
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- •EGFR-TKI molecular response profiling covering 10138 proteins and 13486 mRNAs.
- •EGFR-TKI combination therapy screen using a library of 528 compounds.
- •Several new candidate EGFR-TKI escape mechanisms and combination therapy targets.
- •Combined targeting of the oncogene BCL6 and EGFR results in synergy in NSCLC cells.
20.
《Molecular & cellular proteomics : MCP》2020,19(6):1047-1057
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- •DEqMS is a method for statistical analysis of quantitative MS-data.
- •Variance estimates based on the actual MS-data structure.
- •Improved statistical power and accuracy in protein differential analysis.
- •DEqMS is available as a user-friendly R package in Bioconductor.