共查询到20条相似文献,搜索用时 31 毫秒
1.
《Molecular & cellular proteomics : MCP》2020,19(7):1076-1087
- Download : Download high-res image (233KB)
- Download : Download full-size image
- •Organelle profiling maps capture localizations of 1000s of proteins in one experiment.
- •Comparing maps +/− perturbation reveals disease mechanisms & cellular responses.
- •A conceptual guide to planning and interpreting organellar profiling experiments.
- •A cross-study consensus set of human organellar marker proteins.
2.
《Molecular & cellular proteomics : MCP》2020,19(3):444-455
- Download : Download high-res image (183KB)
- Download : Download full-size image
- •Human spermatozoa possess cells of poor morphology that lack nuclear integrity.
- •These cells can be isolated by density separation.
- •Mass spectrometry reveals their nuclei contain excess protein.
- •TOP2A is a promising marker of this poor nuclear development.
3.
《Molecular & cellular proteomics : MCP》2020,19(4):655-671
- Download : Download high-res image (245KB)
- Download : Download full-size image
- •Acute inhibition of growth-regulatory AGC-kinases Sch9, PKA and Ypk1.
- •Phospho-proteomic profiling of 6373 phsopho-sites.
- •Ypk1 regulates phospho-sites in RRxS/T-context and functionally overlaps with PKA.
- •Ypk1 and PKA activate trehalase activity of Nth1.
4.
Dynamic Transcriptomic and Phosphoproteomic Analysis During Cell Wall Stress in Aspergillus nidulans
Cynthia Chelius Walker Huso Samantha Reese Alexander Doan Stephen Lincoln Kelsi Lawson Bao Tran Raj Purohit Trevor Glaros Ranjan Srivastava Steven D. Harris Mark R. Marten 《Molecular & cellular proteomics : MCP》2020,19(8):1310-1329
5.
《Molecular & cellular proteomics : MCP》2020,19(2):326-343
- Download : Download high-res image (140KB)
- Download : Download full-size image
- •NCMR is crucial for substrate recognition and activity regulation.
- •MASTL conserves a cryptic C-Lobe in the non-conserved middle region.
- •MASTL450 containing the cryptic C-lobe is observed in cancer cell lines.
- •Key phosphorylation sites for MASTL provide an activation model.
6.
《Molecular & cellular proteomics : MCP》2020,19(4):690-700
- Download : Download high-res image (209KB)
- Download : Download full-size image
- •Two molecular groups in anal squamous carcinoma according proteomic profile.
- •Differences in possible targeted processes such as metabolism or immune response.
- •Different percentage of tumor lymphocyte infiltration.
- •Difference in the frequency of ATM variants, related to PPAR inhibitors.
7.
《Molecular & cellular proteomics : MCP》2020,19(6):1005-1016
- Download : Download high-res image (91KB)
- Download : Download full-size image
- •Brain membrane protein extraction.
- •Protein prenylation.
- •Prenyl peptide capture and characterization by LC-MS/MS.
- •HCD and EThcD peptide fragmentation.
8.
《Molecular & cellular proteomics : MCP》2020,19(5):884-899
- Download : Download high-res image (172KB)
- Download : Download full-size image
- •pY phosphoproteomes and dedicated ranking analyses for 16 AML cell lines.
- •RTK drivers, 6 mutant cell lines confirmed, identification for 4 more cell lines.
- •MAPK1/3 phosphorylation for cell lines without TK driver, indicating RAS mutation.
- •Drug target space phosphorylation correlates with drug IC50s in specific cell lines.
9.
《Molecular & cellular proteomics : MCP》2020,19(2):278-293
- Download : Download high-res image (141KB)
- Download : Download full-size image
- •Longitudinal monitoring of B cell subsets shows baseline antibody gene expression.
- •A single, given CDR3 sequence can arise from more than one VJ gene combination.
- •A healthy individual's V gene usage is stable irrespective of infection and subset.
- •Surprisingly, class-switched antibodies can occur early in human B cell development.
10.
《Molecular & cellular proteomics : MCP》2020,19(2):294-307
- Download : Download high-res image (227KB)
- Download : Download full-size image
- •Quantitative proteomes of the cellular surface changes induced by mTORC1 signaling.
- •Hit validation in human cancer cell lines and biopsies.
- •Functional studies showing new drug targets to which cancer cells with hyperactive mTORC1 may be addicted.
- •A new paradigm for drug development, namely targeting cell surface proteins regulated by mTORC1.
11.
《Molecular & cellular proteomics : MCP》2020,19(5):828-838
- Download : Download high-res image (162KB)
- Download : Download full-size image
- •Higher AGC significantly improves quantitation quality in single-cell analysis.
- •The boosting-to-sample ratio should be carefully evaluated and optimized.
- •iBASIL allows for precise quantitation of 1,500 proteins from 104 AML single cells.
- •iBASIL recapitulates major biological differences in different AML single cells.
12.
《Molecular & cellular proteomics : MCP》2020,19(11):1767-1776
- Download : Download high-res image (208KB)
- Download : Download full-size image
- •N-glycan patterns are distinct in pediatric and adult urine.
- •Sex differences of N-glycans are much larger in adults.
- •Pediatric urine has almost no sex differences in N-glycan levels.
- •In adults, the majority of N-glycans were more abundant in males.
13.
《Molecular & cellular proteomics : MCP》2020,19(11):1910-1920
- Download : Download high-res image (194KB)
- Download : Download full-size image
- •PRMT5 glutathionylation is increased in aged mice or under oxidative stress.
- •Deglutathionylation of PRMT5 is catalyzed by glutaredoxin-1.
- •PRMT5 glutathionylation decreases its methyltransferase activity.
- •PRMT5 glutathionylation results in G2/M arrest and inhibits cell proliferation.
14.
《Molecular & cellular proteomics : MCP》2020,19(3):456-466
- Download : Download high-res image (207KB)
- Download : Download full-size image
- •Urinary proteomes of patients with recurrent UTI, renal scarring, and VUR.
- •80 proteins differentially expressed, compared to healthy controls.
- •62 proteins may be indicative of susceptibility for UTI.
- •Altered acute phase response, extracellular matrix and carbohydrate metabolism.
15.
Nataly Mancette Rijensky Netta R. Blondheim Shraga Eilon Barnea Nir Peled Eli Rosenbaum Aron Popovtzer Solomon M. Stemmer Alejandro Livoff Mark Shlapobersky Neta Moskovits Dafna Perry Eitan Rubin Itzhak Haviv Arie Admon 《Molecular & cellular proteomics : MCP》2020,19(8):1360-1374
- Download : Download high-res image (147KB)
- Download : Download full-size image
- •Sufficient tumor tissues are often unavailable large HLA peptidome discovery.
- •Using patient derived xenograft (PDX) tumors can overcome this limitation.
- •The large PDX HLA peptidomes expand significantly those of the original biopsies.
- •The HLA peptidomes of the PDX tumors included many tumor antigens.
16.
《Molecular & cellular proteomics : MCP》2020,19(3):540-553
- Download : Download high-res image (213KB)
- Download : Download full-size image
- •Repeatable quantification of 200 proteins in dried blood spots.
- •Determined lower limit of quantification, repeatability, parallelism and stability.
- •Protein stability in DBS stored at ambient temperatures for up to 2 months.
- •Concentration ranges for 200 proteins in 20 healthy individuals.
17.
《Molecular & cellular proteomics : MCP》2020,19(4):589-607
- Download : Download high-res image (158KB)
- Download : Download full-size image
- •P. aeruginosa grown with exosiderophores and analyzed by proteomic and RT-qPCR.
- •Catechol-type exosiderophores strongly induce the expression of their transporters.
- •Repression of the endogenous iron uptake pathways.
- •Complex phenotypic plasticity in the expression of the various iron-uptake pathways.
18.
《Molecular & cellular proteomics : MCP》2020,19(5):744-756
- Download : Download high-res image (204KB)
- Download : Download full-size image
- •Signaling networks can be highly heterogeneous across cells in a tissue.
- •Various technologies allow analyzing signaling networks at single-cell resolution.
- •The advantages and limitations of each single-cell approach are summarized.
- •Confounding factors in single-cell signaling network analysis are discussed.
19.
《Molecular & cellular proteomics : MCP》2020,19(1):50-64
- Download : Download high-res image (164KB)
- Download : Download full-size image
- •BioID reveals the proximity partners of RSK family members.
- •All RSK isoforms associate with and phosphorylate p120ctn on Ser320.
- •RSK negatively regulates adherens junctions and reduces cell-cell adhesion.
- •p120ctn phosphorylation plays a role in the reorganization of proximity partners.
20.
《Molecular & cellular proteomics : MCP》2020,19(6):916-927
- Download : Download high-res image (222KB)
- Download : Download full-size image
- •Summarize the development of functional protein microarray.
- •Application of functional proteome microarray in basic research.
- •Application of functional proteome microarray in translational research.
- •Fabrication of functional membrane protein array using virion display method.