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1.
Inga Boll Pia Jensen Veit Schwmmle Martin R. Larsen 《Molecular & cellular proteomics : MCP》2020,19(9):1418-1435
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- •Comprehensive sialiomics of isolated rat synaptosomes.
- •Site-specific modulation of sialic acids on surface glycoproteins after brief depolarization.
- •Sialylation as dynamic modification important for synaptic depolarization-dependent processes.
2.
《Molecular & cellular proteomics : MCP》2020,19(3):540-553
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- •Repeatable quantification of 200 proteins in dried blood spots.
- •Determined lower limit of quantification, repeatability, parallelism and stability.
- •Protein stability in DBS stored at ambient temperatures for up to 2 months.
- •Concentration ranges for 200 proteins in 20 healthy individuals.
3.
《Molecular & cellular proteomics : MCP》2020,19(11):1767-1776
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- •N-glycan patterns are distinct in pediatric and adult urine.
- •Sex differences of N-glycans are much larger in adults.
- •Pediatric urine has almost no sex differences in N-glycan levels.
- •In adults, the majority of N-glycans were more abundant in males.
4.
《Molecular & cellular proteomics : MCP》2020,19(7):1236-1247
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- •A predominance of HLA-I bound deamidated peptides are generated through ERAD pathway.
- •Deamidation of peptides with N-glycosylation motifs not in peptidome of HLA-II or proteolysis.
- •Many precursors of ERAD generated deamidated peptides are glycoproteins.
5.
《Molecular & cellular proteomics : MCP》2020,19(3):529-539
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- •N-glycosylation site analysis of the hemipteran pest insect Nilaparvata lugens.
- •Differential N-glycosylation of proteins is observed between male and female adults.
- •Sex-specific glycoproteins are involved in insect reproduction.
6.
Proteomics of Galápagos Marine Iguanas Links Function of Femoral Gland Proteins to the Immune System
Frederik Tellkamp Franziska Lang Alejandro Ibez Lena Abraham Galo Quezada Stefan Günther Mario Looso Fabian Jannik Tann Daniela Müller Franz Cemic Jürgen Hemberger Sebastian Steinfartz Marcus Krüger 《Molecular & cellular proteomics : MCP》2020,19(9):1523-1532
7.
《Molecular & cellular proteomics : MCP》2020,19(11):1876-1895
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- •Guidelines for studying protein complexes via co-fractionation mass spectrometry.
- •A novel procedure for profiling gold standard protein complexes in CF-MS data.
- •Recommendations for efficient CF-MS fractionation collection.
- •Scoring metric recommendations for precise and sensitive CF-MS data analysis.
8.
《Molecular & cellular proteomics : MCP》2020,19(3):490-500
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- •HuProt array-based identification of autoantigens in serum of early lung cancer.
- •Independent validation of early lung cancer biomarker candidates with ELISA.
- •Bioinformatics-aided identification of a biomarker panel.
- •Independent verification of the panel with ELISA and immunohistochemistry.
9.
《Molecular & cellular proteomics : MCP》2020,19(6):1005-1016
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- •Brain membrane protein extraction.
- •Protein prenylation.
- •Prenyl peptide capture and characterization by LC-MS/MS.
- •HCD and EThcD peptide fragmentation.
10.
Yi Liu Michael J. Trnka Shenheng Guan Doyoung Kwon Do-Hyung Kim J.-J. Chen Peter A. Greer A. L. Burlingame Maria Almira Correia 《Molecular & cellular proteomics : MCP》2020,19(12):1968-1986
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- •Liver Mallory-Denk-Body inducers elicited an IκBα-loss and NF-κB-activation.
- •IκBα-loss was due to its sequestration into insoluble cytoplasmic aggregates.
- •Four proteomic approaches identified 10 IκBα-interacting/aggregating proteins.
- •Nup153/RanBP2-aggregation prevented IκBα nuclear entry for ending NF-κB-activation.
11.
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- •The gut mucosal-luminal interface is a spatiotemporal heterogeneous ecosystem.
- •Proteomics and metaproteomics are tools to study the host and microbiome functionality.
- •Insights into functional diversity, biomass, and matter flow can be obtained.
- •Such data can be complementary inputs for building ecology models of the microbiome.
12.
《Molecular & cellular proteomics : MCP》2020,19(1):1-10
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- •Co-elution stands out as a global interactome mapping method.
- •Benefits include all-to-all protein analysis and measurement of interactome perturbations.
- •Different separation, quantification and bioinformatic strategies are available.
- •Design considerations depend largely on system under study.
13.
Eugen Netz Tjeerd M. H. Dijkstra Timo Sachsenberg Lukas Zimmermann Mathias Walzer Thomas Monecke Ralf Ficner Olexandr Dybkov Henning Urlaub Oliver Kohlbacher 《Molecular & cellular proteomics : MCP》2020,19(12):2157-2168
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- •OpenPepXL is a new XL-MS identification tool with a high sensitivity.
- •It is available for all common operating systems and remote computing environments.
- •OpenPepXL is open source and supports open OpenPepXL is available as part of OpenMS data formats like mzML and mzIdentML.
- •at https://www.openms.de/openpepxl.
14.
Matthias Schittmayer Nemanja Vujic Barbara Darnhofer Melanie Korbelius Sophie Honeder Dagmar Kratky Ruth Birner-Gruenberger 《Molecular & cellular proteomics : MCP》2020,19(12):2104-2115
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- •Activity based serine hydrolase profiles in 11 fractions along the murine small intestine.
- •AADAC and Ces2e activity profiles correlate with chylomicron secretion.
- •Ces2e shows the highest activity based enrichment in the entire small intestine.
15.
Yi-Han Lin Maryann P. Platt Haiyan Fu Yuan Gui Yanlin Wang Norberto Gonzalez-Juarbe Dong Zhou Yanbao Yu 《Molecular & cellular proteomics : MCP》2020,19(12):2030-2047
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- •Cecal Ligation Puncture (CLP) mouse model to study sepsis-induced kidney disease.
- •Quantitative global proteome and phosphoproteome profiling of mouse kidneys.
- •Highly significant candidate markers for onset and progression of AKI to CKD.
- •Mechanistic insights into sepsis-associated kidney injuries.
16.
Jana Zecha Chien-Yun Lee Florian P. Bayer Chen Meng Vincent Grass Johannes Zerweck Karsten Schnatbaum Thomas Michler Andreas Pichlmair Christina Ludwig Bernhard Kuster 《Molecular & cellular proteomics : MCP》2020,19(9):1503-1522
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- •In-depth proteomes of 4 SARS-CoV-2 cell line models (Vero E6, Calu-3, Caco-2, A549).
- •Proteomic evidence for thousands of Chlorocebus sabaeus proteins.
- •Proteomic response of Vero E6 cells to SARS-CoV-2 infection.
- •Synthetic peptides, spectral libraries, and targeted assays for SARS-CoV-2 proteins.
17.
《Molecular & cellular proteomics : MCP》2020,19(1):114-127
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- •Quantitative proteomics and machine learning to study plasma biomarkers in HCM.
- •Six peptides are increased in plasma of LVH+ HCM compared to controls.
- •Peptide biomarkers correlate with imaging markers of phenotype severity.
- •Peptide biomarkers correlate with the estimated sudden cardiac death risk.
18.
《Molecular & cellular proteomics : MCP》2020,19(11):1760-1766
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- •Future proteomic analyses for longitudinal studies and P4 medicine arguably require ≥1M samples/day.
- •Proteome depth/coverage is commonly the focus whereas analytical speed is typically neglected.
- •A compromise between analytical depth and speed is needed for future large-scale studies.
- •Ultrahigh-speed ‘omic’ analyses require tools that are intrinsically fast such as laser-based MS.
19.
《Molecular & cellular proteomics : MCP》2020,19(7):1070-1075
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- •Peptide-based screens provide a scalable approach to study protein-protein interactions.
- •These screens help to characterize the function of structurally disordered regions.
- •The impact of posttranslational modifications can be directly investigated.
20.
《Molecular & cellular proteomics : MCP》2020,19(5):828-838
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- •Higher AGC significantly improves quantitation quality in single-cell analysis.
- •The boosting-to-sample ratio should be carefully evaluated and optimized.
- •iBASIL allows for precise quantitation of 1,500 proteins from 104 AML single cells.
- •iBASIL recapitulates major biological differences in different AML single cells.