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《Molecular & cellular proteomics : MCP》2020,19(6):944-959
Highlights
- •Automated statistical approach for detecting uninformative features and outliers.
- •Improved performance on relative protein quantification.
- •An option in the open-source R-based software MSstats.
3.
《Molecular & cellular proteomics : MCP》2020,19(7):1104-1119
Highlights
- •Global and targeted phosphoproteomics in RICTOR-deficient brown adipocytes.
- •RICTOR loss leads to higher levels of many interferon response-associated proteins.
- •RICTOR loss dampens the dynamic insulin-dependent phosphoproteome response.
- •ACLY S455, VIM S39, and EIF4B S422 are among the most dampened phosphosites.
4.
Jana Zecha Chien-Yun Lee Florian P. Bayer Chen Meng Vincent Grass Johannes Zerweck Karsten Schnatbaum Thomas Michler Andreas Pichlmair Christina Ludwig Bernhard Kuster 《Molecular & cellular proteomics : MCP》2020,19(9):1503-1522
Highlights
- •In-depth proteomes of 4 SARS-CoV-2 cell line models (Vero E6, Calu-3, Caco-2, A549).
- •Proteomic evidence for thousands of Chlorocebus sabaeus proteins.
- •Proteomic response of Vero E6 cells to SARS-CoV-2 infection.
- •Synthetic peptides, spectral libraries, and targeted assays for SARS-CoV-2 proteins.
5.
《Molecular & cellular proteomics : MCP》2020,19(11):1767-1776
Highlights
- •N-glycan patterns are distinct in pediatric and adult urine.
- •Sex differences of N-glycans are much larger in adults.
- •Pediatric urine has almost no sex differences in N-glycan levels.
- •In adults, the majority of N-glycans were more abundant in males.
6.
《Molecular & cellular proteomics : MCP》2020,19(11):1805-1825
Highlights
- •Ectopic ATP synthase as a therapeutic target for gefitinib-resistant NSCLC.
- •Multiomics uncovers the dynamic network in response to ecto-ATP synthase blockade.
- •Ecto-ATP synthase blockade induces cytotoxicity by CK2/phospho-topo IIα/GAS5 axis.
- •A positive feedforward circuit between phospho-topo IIα and lncRNA-GAS5.
7.
《Molecular & cellular proteomics : MCP》2020,19(6):928-943
Highlights
- •EGFR-TKI molecular response profiling covering 10138 proteins and 13486 mRNAs.
- •EGFR-TKI combination therapy screen using a library of 528 compounds.
- •Several new candidate EGFR-TKI escape mechanisms and combination therapy targets.
- •Combined targeting of the oncogene BCL6 and EGFR results in synergy in NSCLC cells.
8.
Temporal Quantitative Proteomics of mGluR-induced Protein Translation and Phosphorylation in Neurons
Charlotte A. G. H. van Gelder Renske Penning Tim S. Veth Lisa A. E. Catsburg Casper C. Hoogenraad Harold D. MacGillavry Maarten Altelaar 《Molecular & cellular proteomics : MCP》2020,19(12):1952-1968
Highlights
- •Integrated phosphoproteomics and analyses of newly synthesized proteins in neurons.
- •Resource of temporal mGluR-induced signaling pathways upon DHPG stimulation.
- •Validation of PKC, MAPK1, CAMKIIa, and CDK2 in mGluR-activation and signaling.
- •Validation of Intersectin-1 in DHPG-induced AMPAR internalization.
9.
《Molecular & cellular proteomics : MCP》2020,19(7):1193-1208
Highlights
- •cGAS acetylations and phosphorylations under basal and immune-stimulated states.
- •K384 and K414 acetylations and S305 phosphorylation inhibit cGAS-mediated apoptosis.
- •Acetylation at K198 stimulates cGAS-dependent interferon signaling.
- •K198 acetylation is decreased upon herpesvirus infection.
10.
《Molecular & cellular proteomics : MCP》2020,19(11):1910-1920
Highlights
- •PRMT5 glutathionylation is increased in aged mice or under oxidative stress.
- •Deglutathionylation of PRMT5 is catalyzed by glutaredoxin-1.
- •PRMT5 glutathionylation decreases its methyltransferase activity.
- •PRMT5 glutathionylation results in G2/M arrest and inhibits cell proliferation.
11.
《Molecular & cellular proteomics : MCP》2020,19(3):540-553
Highlights
- •Repeatable quantification of 200 proteins in dried blood spots.
- •Determined lower limit of quantification, repeatability, parallelism and stability.
- •Protein stability in DBS stored at ambient temperatures for up to 2 months.
- •Concentration ranges for 200 proteins in 20 healthy individuals.
12.
《Molecular & cellular proteomics : MCP》2020,19(1):128-141
Highlights
- •Laser microdissection of highly vulnerable hippocampal region.
- •Proteomic analysis of postmortem human brain tissue of AD and control cases.
- •Decreased levels of presynaptic proteins, but not postsynaptic proteins, in AD.
- •Immunohistochemistry verifies decreased levels of selected presynaptic proteins.
13.
《Molecular & cellular proteomics : MCP》2020,19(3):456-466
Highlights
- •Urinary proteomes of patients with recurrent UTI, renal scarring, and VUR.
- •80 proteins differentially expressed, compared to healthy controls.
- •62 proteins may be indicative of susceptibility for UTI.
- •Altered acute phase response, extracellular matrix and carbohydrate metabolism.
14.
《Molecular & cellular proteomics : MCP》2020,19(1):114-127
Highlights
- •Quantitative proteomics and machine learning to study plasma biomarkers in HCM.
- •Six peptides are increased in plasma of LVH+ HCM compared to controls.
- •Peptide biomarkers correlate with imaging markers of phenotype severity.
- •Peptide biomarkers correlate with the estimated sudden cardiac death risk.
15.
《Molecular & cellular proteomics : MCP》2020,19(6):1005-1016
Highlights
- •Brain membrane protein extraction.
- •Protein prenylation.
- •Prenyl peptide capture and characterization by LC-MS/MS.
- •HCD and EThcD peptide fragmentation.
16.
《Molecular & cellular proteomics : MCP》2020,19(1):31-49
Highlights
- •Immunopeptidomics bears the potential to link diseases to antigen representation.
- •We suggest to achieve this by analyzing the immunopeptidomes of cohorts of patients.
- •Current mass spectrometry-based techniques to analyze immunopeptidomes are described.
- •We term the proposed approach “Immunopeptidome-wide association studies” (IWAS).
17.
《Molecular & cellular proteomics : MCP》2020,19(1):1-10
Highlights
- •Co-elution stands out as a global interactome mapping method.
- •Benefits include all-to-all protein analysis and measurement of interactome perturbations.
- •Different separation, quantification and bioinformatic strategies are available.
- •Design considerations depend largely on system under study.
18.
《Molecular & cellular proteomics : MCP》2020,19(4):690-700
Highlights
- •Two molecular groups in anal squamous carcinoma according proteomic profile.
- •Differences in possible targeted processes such as metabolism or immune response.
- •Different percentage of tumor lymphocyte infiltration.
- •Difference in the frequency of ATM variants, related to PPAR inhibitors.
19.
《Molecular & cellular proteomics : MCP》2020,19(2):224-232
Highlights
- •Lectins and glycan-binding antibodies are valuable as probe of glycans.
- •Advanced bioinformatics tools enable the mining of glycan-array data.
- •New insights into protein-glycan interactions have value in biological research.
20.
《Molecular & cellular proteomics : MCP》2020,19(5):828-838
Highlights
- •Higher AGC significantly improves quantitation quality in single-cell analysis.
- •The boosting-to-sample ratio should be carefully evaluated and optimized.
- •iBASIL allows for precise quantitation of 1,500 proteins from 104 AML single cells.
- •iBASIL recapitulates major biological differences in different AML single cells.