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1.
《Molecular & cellular proteomics : MCP》2020,19(11):1910-1920
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- •PRMT5 glutathionylation is increased in aged mice or under oxidative stress.
- •Deglutathionylation of PRMT5 is catalyzed by glutaredoxin-1.
- •PRMT5 glutathionylation decreases its methyltransferase activity.
- •PRMT5 glutathionylation results in G2/M arrest and inhibits cell proliferation.
2.
《Molecular & cellular proteomics : MCP》2020,19(11):1767-1776
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- •N-glycan patterns are distinct in pediatric and adult urine.
- •Sex differences of N-glycans are much larger in adults.
- •Pediatric urine has almost no sex differences in N-glycan levels.
- •In adults, the majority of N-glycans were more abundant in males.
3.
《Molecular & cellular proteomics : MCP》2020,19(1):114-127
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- •Quantitative proteomics and machine learning to study plasma biomarkers in HCM.
- •Six peptides are increased in plasma of LVH+ HCM compared to controls.
- •Peptide biomarkers correlate with imaging markers of phenotype severity.
- •Peptide biomarkers correlate with the estimated sudden cardiac death risk.
4.
《Molecular & cellular proteomics : MCP》2020,19(3):456-466
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- •Urinary proteomes of patients with recurrent UTI, renal scarring, and VUR.
- •80 proteins differentially expressed, compared to healthy controls.
- •62 proteins may be indicative of susceptibility for UTI.
- •Altered acute phase response, extracellular matrix and carbohydrate metabolism.
5.
《Molecular & cellular proteomics : MCP》2020,19(2):421-430
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- •Modern DIA methods contain high quality MS1 and MS2.
- •We developed a statistical procedure incorporating MS1 and MS2.
- •Benchmarking, the combined method outperformed the individual use of MS1 or MS2.
6.
7.
《Molecular & cellular proteomics : MCP》2020,19(6):1005-1016
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- •Brain membrane protein extraction.
- •Protein prenylation.
- •Prenyl peptide capture and characterization by LC-MS/MS.
- •HCD and EThcD peptide fragmentation.
8.
zge Karayel Francesca Tonelli Sebastian Virreira Winter Phillip E. Geyer Ying Fan Esther M. Sammler Dario R. Alessi Martin Steger Matthias Mann 《Molecular & cellular proteomics : MCP》2020,19(9):1546-1560
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- •MS-based clinical assay that accurately determines phospho Rab10 occupancy.
- •Stable isotope labeled phosphopeptide injected as a standard with endogenous tryptic phospho Rab peptide for accurate ratio determination.
- •Determination of pRab levels in neutrophils of Parkinson disease patients.
- •Relevance of pRab levels as marker of PD.
9.
《Molecular & cellular proteomics : MCP》2020,19(2):233-244
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- •Mapping kinase-substrate relationships is vital in discovering new tuberculosis drug targets.
- •LC-MS/MS-based phosphoproteomics expand mycobacterial STPK substrate catalogues.
- •We review and integrate MS-generated datasets on novel candidate substrates.
- •Validation studies are necessary to confirm true physiological substrates of STPKs.
10.
《Molecular & cellular proteomics : MCP》2020,19(2):261-277
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- •We used phosphoproteomics to reveal the underlying mechanisms of drug synergy on EGFR and ROCK co-inhibition in TNBC cells.
- •EGFR inhibition alone induces autophagy activation in TNBC cells as a cytoprotective mechanism.
- •Combinatorial treatment leads to impaired autophagic flux resulting in a strong accumulation of autophagic vacuoles.
- •We hypothesize that ROCKi-induced cytoskeletal changes impair autophagosome clearance ultimately leading to cell death.
11.
《Molecular & cellular proteomics : MCP》2020,19(5):757-773
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- •Proximity-dependent biotinylation (PDB) approaches involve fusion of a bait with an enzyme.
- •BioID (biotin protein ligase) and APEX (peroxidase) are distinct enzymes used in PDB.
- •Past, present and future development and applications of PDB are discussed.
- •We review labeling mechanisms and kinetics to provide guidance for experimental design.
- •We discuss controls and considerations for data interpretation.
12.
《Molecular & cellular proteomics : MCP》2020,19(5):839-851
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- •A detailed investigation of the protein extraction step from FFPE tissue is shown.
- •Acidification during peptide wash increased peptide recovery of the SP3 method.
- •LCM of substantia nigra enriched neuron-specific proteins including TH.
- •>5,600 proteins were quantified using 3000 cells per sample from substantia nigra.
13.
Nataly Mancette Rijensky Netta R. Blondheim Shraga Eilon Barnea Nir Peled Eli Rosenbaum Aron Popovtzer Solomon M. Stemmer Alejandro Livoff Mark Shlapobersky Neta Moskovits Dafna Perry Eitan Rubin Itzhak Haviv Arie Admon 《Molecular & cellular proteomics : MCP》2020,19(8):1360-1374
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- •Sufficient tumor tissues are often unavailable large HLA peptidome discovery.
- •Using patient derived xenograft (PDX) tumors can overcome this limitation.
- •The large PDX HLA peptidomes expand significantly those of the original biopsies.
- •The HLA peptidomes of the PDX tumors included many tumor antigens.
14.
Yi-Han Lin Maryann P. Platt Haiyan Fu Yuan Gui Yanlin Wang Norberto Gonzalez-Juarbe Dong Zhou Yanbao Yu 《Molecular & cellular proteomics : MCP》2020,19(12):2030-2047
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- •Cecal Ligation Puncture (CLP) mouse model to study sepsis-induced kidney disease.
- •Quantitative global proteome and phosphoproteome profiling of mouse kidneys.
- •Highly significant candidate markers for onset and progression of AKI to CKD.
- •Mechanistic insights into sepsis-associated kidney injuries.
15.
《Molecular & cellular proteomics : MCP》2020,19(5):828-838
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- •Higher AGC significantly improves quantitation quality in single-cell analysis.
- •The boosting-to-sample ratio should be carefully evaluated and optimized.
- •iBASIL allows for precise quantitation of 1,500 proteins from 104 AML single cells.
- •iBASIL recapitulates major biological differences in different AML single cells.
16.
《Molecular & cellular proteomics : MCP》2020,19(7):1120-1131
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- •Quantitative proteomics of isolated lysosomes, autophagosomes and proteasomes.
- •Pharmacological inhibition of proteasomes leads to their accumulation within lysosomes.
- •Inhibition of classical autophagy pathways cannot completely block this process.
- •Known autophagy adaptor proteins are not involved.
17.
《Molecular & cellular proteomics : MCP》2020,19(3):540-553
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- •Repeatable quantification of 200 proteins in dried blood spots.
- •Determined lower limit of quantification, repeatability, parallelism and stability.
- •Protein stability in DBS stored at ambient temperatures for up to 2 months.
- •Concentration ranges for 200 proteins in 20 healthy individuals.
18.
《Molecular & cellular proteomics : MCP》2020,19(3):444-455
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- •Human spermatozoa possess cells of poor morphology that lack nuclear integrity.
- •These cells can be isolated by density separation.
- •Mass spectrometry reveals their nuclei contain excess protein.
- •TOP2A is a promising marker of this poor nuclear development.
19.
《Molecular & cellular proteomics : MCP》2020,19(4):690-700
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- •Two molecular groups in anal squamous carcinoma according proteomic profile.
- •Differences in possible targeted processes such as metabolism or immune response.
- •Different percentage of tumor lymphocyte infiltration.
- •Difference in the frequency of ATM variants, related to PPAR inhibitors.
20.
《Molecular & cellular proteomics : MCP》2020,19(4):589-607
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- •P. aeruginosa grown with exosiderophores and analyzed by proteomic and RT-qPCR.
- •Catechol-type exosiderophores strongly induce the expression of their transporters.
- •Repression of the endogenous iron uptake pathways.
- •Complex phenotypic plasticity in the expression of the various iron-uptake pathways.