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1.
《Molecular & cellular proteomics : MCP》2020,19(1):50-64
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- •BioID reveals the proximity partners of RSK family members.
- •All RSK isoforms associate with and phosphorylate p120ctn on Ser320.
- •RSK negatively regulates adherens junctions and reduces cell-cell adhesion.
- •p120ctn phosphorylation plays a role in the reorganization of proximity partners.
2.
《Molecular & cellular proteomics : MCP》2020,19(6):916-927
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- •Summarize the development of functional protein microarray.
- •Application of functional proteome microarray in basic research.
- •Application of functional proteome microarray in translational research.
- •Fabrication of functional membrane protein array using virion display method.
3.
《Molecular & cellular proteomics : MCP》2020,19(7):1070-1075
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- •Peptide-based screens provide a scalable approach to study protein-protein interactions.
- •These screens help to characterize the function of structurally disordered regions.
- •The impact of posttranslational modifications can be directly investigated.
4.
A Quantitative Tri-fluorescent Yeast Two-hybrid System: From Flow Cytometry to In cellula Affinities
《Molecular & cellular proteomics : MCP》2020,19(4):701-715
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- •Simultaneous quantification of Bait, Prey and Reporter at the single cell level.
- •Two hours of reaction are enough instead of 24–48 h for conventional assays.
- •Potential expression problems of the Bait and Prey can be easily detected.
- •True positive PPIs feature a distinct pattern of Reporter level versus Bait/Prey level.
- •PPIs with unknown affinities can be ranked using an affinity ladder.
5.
《Molecular & cellular proteomics : MCP》2020,19(1):1-10
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- •Co-elution stands out as a global interactome mapping method.
- •Benefits include all-to-all protein analysis and measurement of interactome perturbations.
- •Different separation, quantification and bioinformatic strategies are available.
- •Design considerations depend largely on system under study.
6.
《Molecular & cellular proteomics : MCP》2020,19(11):1876-1895
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- •Guidelines for studying protein complexes via co-fractionation mass spectrometry.
- •A novel procedure for profiling gold standard protein complexes in CF-MS data.
- •Recommendations for efficient CF-MS fractionation collection.
- •Scoring metric recommendations for precise and sensitive CF-MS data analysis.
7.
《Molecular & cellular proteomics : MCP》2020,19(3):554-568
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- •New quality assessment metrics to evaluate proteome-wide cross-linking mass spectrometry (XL-MS) data sets.
- •New “MS3-centric” cross-link search engine named MaXLinker with high sensitivity and specificity.
- •More than 9300 cross-links from our human proteome-wide XL-MS study.
- •Orthogonal experimental validation of novel interactions identified in our study.
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9.
《Molecular & cellular proteomics : MCP》2020,19(3):467-477
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- •Each component of the AMPK trimeric complex was profiled by interaction proteomics.
- •The subunit composition of the AMPK complex directs interactions to distinct proteins.
- •AMPK interacts with Artemis and plays a role in Non-Homologous End Joining.
10.
《Molecular & cellular proteomics : MCP》2020,19(4):655-671
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- •Acute inhibition of growth-regulatory AGC-kinases Sch9, PKA and Ypk1.
- •Phospho-proteomic profiling of 6373 phsopho-sites.
- •Ypk1 regulates phospho-sites in RRxS/T-context and functionally overlaps with PKA.
- •Ypk1 and PKA activate trehalase activity of Nth1.
11.
《Molecular & cellular proteomics : MCP》2020,19(5):757-773
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- •Proximity-dependent biotinylation (PDB) approaches involve fusion of a bait with an enzyme.
- •BioID (biotin protein ligase) and APEX (peroxidase) are distinct enzymes used in PDB.
- •Past, present and future development and applications of PDB are discussed.
- •We review labeling mechanisms and kinetics to provide guidance for experimental design.
- •We discuss controls and considerations for data interpretation.
12.
《Molecular & cellular proteomics : MCP》2020,19(2):308-325
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- •Multi-omics analysis on mode of action of novel antimalarial, JPC-3210
- •JPC-3210 has rapid parasite killing kinetics.
- •Metabolomics and peptidomics demonstrated JPC-3210 inhibits hemoglobin digestion.
- •Proteomics demonstrated JPC-3210 enriches for translation regulation proteins.
13.
《Molecular & cellular proteomics : MCP》2020,19(6):1005-1016
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- •Brain membrane protein extraction.
- •Protein prenylation.
- •Prenyl peptide capture and characterization by LC-MS/MS.
- •HCD and EThcD peptide fragmentation.
14.
Anna M. Schmoker Jaye L. Weinert Jacob M. Markwood Kathryn S. Albretsen Michelle L. Lunde Marion E. Weir Alicia M. Ebert Karen L. Hinkle Bryan A. Ballif 《Molecular & cellular proteomics : MCP》2020,19(10):1586-1601
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- •FYN and ABL differentially regulate DCBLD Ser/Thr/Tyr phosphorylation.
- •DCBLD1 and DCBLD2 interactomes are modulated by FYN and ABL.
- •ABL drives a direct DCBLD2/14-3-3 interaction.
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16.
Jana Zecha Chien-Yun Lee Florian P. Bayer Chen Meng Vincent Grass Johannes Zerweck Karsten Schnatbaum Thomas Michler Andreas Pichlmair Christina Ludwig Bernhard Kuster 《Molecular & cellular proteomics : MCP》2020,19(9):1503-1522
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- •In-depth proteomes of 4 SARS-CoV-2 cell line models (Vero E6, Calu-3, Caco-2, A549).
- •Proteomic evidence for thousands of Chlorocebus sabaeus proteins.
- •Proteomic response of Vero E6 cells to SARS-CoV-2 infection.
- •Synthetic peptides, spectral libraries, and targeted assays for SARS-CoV-2 proteins.
17.
《Molecular & cellular proteomics : MCP》2020,19(6):971-993
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- •db/db β-cells restores appropriate insulin stores and normalize secretory function.
- •Numerous changes in the phosphorylation and sialylation states by euglycemic rest.
- •Restoration of numerous dysfunctional biological processes following euglycemic rest.
- •β-cell adaptive flexibility may lead to improvement in endogenous β-cell function.
18.
《Molecular & cellular proteomics : MCP》2020,19(4):640-654
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- •Quantitative proteome interactions with 5 different C9ORF72 dipolypeptides (DPRs).
- •The arg-rich DPRs promiscuously bound to the proteome compared with the other DPRs.
- •Long repeat lengths of arg-rich DPRs, but not short lengths, stalled ribosomes.
- •The arg-rich DPRs also reduced arginine methylation and actin cytoskeleton assembly.
19.
《Molecular & cellular proteomics : MCP》2020,19(5):828-838
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- •Higher AGC significantly improves quantitation quality in single-cell analysis.
- •The boosting-to-sample ratio should be carefully evaluated and optimized.
- •iBASIL allows for precise quantitation of 1,500 proteins from 104 AML single cells.
- •iBASIL recapitulates major biological differences in different AML single cells.
20.
《Molecular & cellular proteomics : MCP》2020,19(3):456-466
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- •Urinary proteomes of patients with recurrent UTI, renal scarring, and VUR.
- •80 proteins differentially expressed, compared to healthy controls.
- •62 proteins may be indicative of susceptibility for UTI.
- •Altered acute phase response, extracellular matrix and carbohydrate metabolism.