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1.
《Molecular & cellular proteomics : MCP》2020,19(6):1005-1016
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- •Brain membrane protein extraction.
- •Protein prenylation.
- •Prenyl peptide capture and characterization by LC-MS/MS.
- •HCD and EThcD peptide fragmentation.
2.
《Molecular & cellular proteomics : MCP》2020,19(6):994-1004
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- •HLA-B*27:05 and ERAP2 are risk factors for ankylosing spondylitis.
- •The effects of ERAP2 on the B*27:05 ligandome are defined.
- •P1, P2, P3, P7, and PΩ peptide positions are influenced by ERAP2.
- •These effects provide a basis for the association of ERAP2 with the autoimmune disease.
3.
《Molecular & cellular proteomics : MCP》2020,19(6):1047-1057
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- •DEqMS is a method for statistical analysis of quantitative MS-data.
- •Variance estimates based on the actual MS-data structure.
- •Improved statistical power and accuracy in protein differential analysis.
- •DEqMS is available as a user-friendly R package in Bioconductor.
4.
Jack William Houghton Guy Carpenter Joachim Hans Manuel Pesaro Steven Lynham Gordon Proctor 《Molecular & cellular proteomics : MCP》2020,19(10):1664-1676
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- •Salivary secretion was increased by mouth rinsing with TRP channel agonists.
- •The salivary proteome varied over time and was changed by TRP channel stimulation.
- •Immunoreactive Cystatin S was increased in saliva after TRPV1 stimulation.
5.
Yi-Han Lin Maryann P. Platt Haiyan Fu Yuan Gui Yanlin Wang Norberto Gonzalez-Juarbe Dong Zhou Yanbao Yu 《Molecular & cellular proteomics : MCP》2020,19(12):2030-2047
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- •Cecal Ligation Puncture (CLP) mouse model to study sepsis-induced kidney disease.
- •Quantitative global proteome and phosphoproteome profiling of mouse kidneys.
- •Highly significant candidate markers for onset and progression of AKI to CKD.
- •Mechanistic insights into sepsis-associated kidney injuries.
6.
《Molecular & cellular proteomics : MCP》2020,19(11):1896-1909
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- •Epitope-tagging of a proteasome subunit allows for facile immuno-isolation.
- •An engineered yeast strain permits capture of proteasome-associated substrates.
- •MS/MS identified all 33 resident proteasome subunits in the 20S and 19S particles.
- •Analysis of associated proteins and characterization of newly identified ERAD substrate.
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8.
《Molecular & cellular proteomics : MCP》2020,19(11):1910-1920
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- •PRMT5 glutathionylation is increased in aged mice or under oxidative stress.
- •Deglutathionylation of PRMT5 is catalyzed by glutaredoxin-1.
- •PRMT5 glutathionylation decreases its methyltransferase activity.
- •PRMT5 glutathionylation results in G2/M arrest and inhibits cell proliferation.
9.
Prashali Bansal Johannes Madlung Kristina Schaaf Boris Macek Fulvia Bono 《Molecular & cellular proteomics : MCP》2020,19(9):1485-1502
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- •Label-free and dimethyl labeling MS analysis of 6 RBPs from Drosophila ovaries.
- •Functionally related RBPs show overlapping proteomes.
- •Selective co-purification of splicing factors and translational regulators.
- •Validation of 26 novel interactions by co-immunoprecipitation.
10.
《Molecular & cellular proteomics : MCP》2020,19(6):1058-1069
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- •Highly parallelizable 4D feature detection in ion mobility enhanced shotgun proteomics.
- •Multidimensional non-linear mass, retention time and ion mobility recalibration.
- •Collision cross section aware matching between runs.
- •Label-free quantification of ion mobility MS data.
11.
《Molecular & cellular proteomics : MCP》2020,19(11):1767-1776
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- •N-glycan patterns are distinct in pediatric and adult urine.
- •Sex differences of N-glycans are much larger in adults.
- •Pediatric urine has almost no sex differences in N-glycan levels.
- •In adults, the majority of N-glycans were more abundant in males.
12.
《Molecular & cellular proteomics : MCP》2020,19(4):716-729
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- •Increased proteome coverage with Orbitrap Exploris 480 MS and FAIMS using single compensation voltages and short LC gradients.
- •Towards single-cell proteomics with high-sensitivity analysis of 5 ng HeLa with more than 1,000 proteins identified in 5 minutes using FAIMS and DIA.
- •Deep proteome profiling across twelve rat organs tissues by label-free quantitation using DIA compared to TMT-multiplexing and turboTMT acquisition using phi-SDM.
- •Rapid and sensitive phosphoproteomics with automated enrichment using Ti-IMAC magnetic beads and direct DIA analysis.
13.
《Molecular & cellular proteomics : MCP》2020,19(1):114-127
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- •Quantitative proteomics and machine learning to study plasma biomarkers in HCM.
- •Six peptides are increased in plasma of LVH+ HCM compared to controls.
- •Peptide biomarkers correlate with imaging markers of phenotype severity.
- •Peptide biomarkers correlate with the estimated sudden cardiac death risk.
14.
《Molecular & cellular proteomics : MCP》2020,19(11):1760-1766
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- •Future proteomic analyses for longitudinal studies and P4 medicine arguably require ≥1M samples/day.
- •Proteome depth/coverage is commonly the focus whereas analytical speed is typically neglected.
- •A compromise between analytical depth and speed is needed for future large-scale studies.
- •Ultrahigh-speed ‘omic’ analyses require tools that are intrinsically fast such as laser-based MS.
15.
《Molecular & cellular proteomics : MCP》2020,19(3):456-466
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- •Urinary proteomes of patients with recurrent UTI, renal scarring, and VUR.
- •80 proteins differentially expressed, compared to healthy controls.
- •62 proteins may be indicative of susceptibility for UTI.
- •Altered acute phase response, extracellular matrix and carbohydrate metabolism.
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17.
zge Karayel Francesca Tonelli Sebastian Virreira Winter Phillip E. Geyer Ying Fan Esther M. Sammler Dario R. Alessi Martin Steger Matthias Mann 《Molecular & cellular proteomics : MCP》2020,19(9):1546-1560
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- •MS-based clinical assay that accurately determines phospho Rab10 occupancy.
- •Stable isotope labeled phosphopeptide injected as a standard with endogenous tryptic phospho Rab peptide for accurate ratio determination.
- •Determination of pRab levels in neutrophils of Parkinson disease patients.
- •Relevance of pRab levels as marker of PD.
18.
《Molecular & cellular proteomics : MCP》2020,19(3):432-443
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- •Validation of an omic method for antigen identification using LC-MS/MS.
- •Validation of accuracy, precision, specificity, limit of detection and robustness.
- •Validation according to the current FDA and EMA guidelines.
19.
《Molecular & cellular proteomics : MCP》2020,19(3):554-568
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- •New quality assessment metrics to evaluate proteome-wide cross-linking mass spectrometry (XL-MS) data sets.
- •New “MS3-centric” cross-link search engine named MaXLinker with high sensitivity and specificity.
- •More than 9300 cross-links from our human proteome-wide XL-MS study.
- •Orthogonal experimental validation of novel interactions identified in our study.
20.
《Molecular & cellular proteomics : MCP》2020,19(3):444-455
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- •Human spermatozoa possess cells of poor morphology that lack nuclear integrity.
- •These cells can be isolated by density separation.
- •Mass spectrometry reveals their nuclei contain excess protein.
- •TOP2A is a promising marker of this poor nuclear development.