共查询到20条相似文献,搜索用时 31 毫秒
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Eduard Hofsetz Fatih Demir Karolina Szczepanowska Alexandra Kukat Jayachandran N. Kizhakkedathu Aleksandra Trifunovic Pitter F. Huesgen 《Molecular & cellular proteomics : MCP》2020,19(8):1330-1345
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- •Mitochondrial heart N terminome shows aminopeptidase processing after MTS cleavage.
- •CLPP-deficiency alters protein processing patterns in mouse heart mitochondria.
- •Candidate substrates identified by N termini accumulation and interaction with inactive ClpXP.
- •UQCRC1, HSPA9 and OAT validated biochemically as high confidence ClpXP substrates.
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Anna M. Schmoker Jaye L. Weinert Jacob M. Markwood Kathryn S. Albretsen Michelle L. Lunde Marion E. Weir Alicia M. Ebert Karen L. Hinkle Bryan A. Ballif 《Molecular & cellular proteomics : MCP》2020,19(10):1586-1601
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- •FYN and ABL differentially regulate DCBLD Ser/Thr/Tyr phosphorylation.
- •DCBLD1 and DCBLD2 interactomes are modulated by FYN and ABL.
- •ABL drives a direct DCBLD2/14-3-3 interaction.
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《Molecular & cellular proteomics : MCP》2020,19(7):1193-1208
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- •cGAS acetylations and phosphorylations under basal and immune-stimulated states.
- •K384 and K414 acetylations and S305 phosphorylation inhibit cGAS-mediated apoptosis.
- •Acetylation at K198 stimulates cGAS-dependent interferon signaling.
- •K198 acetylation is decreased upon herpesvirus infection.
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Elez D. Vainer Juliane Kania-Almog Ghadeer Zatara Yishai Levin Gilad W. Vainer 《Molecular & cellular proteomics : MCP》2020,19(10):1619-1631
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- •TOP: robust, bio-friendly FFPE proteome extraction method with less fixation bias.
- •Proteome of MSI-H colorectal cancer identifies immunobiology key elements.
- •MSI-H tumor displays an “INFg-STAT1 centric signature”.
- •Long-term IFNg induction In-vitro mimicks MSI-H signature.
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《Molecular & cellular proteomics : MCP》2020,19(7):1076-1087
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- •Organelle profiling maps capture localizations of 1000s of proteins in one experiment.
- •Comparing maps +/− perturbation reveals disease mechanisms & cellular responses.
- •A conceptual guide to planning and interpreting organellar profiling experiments.
- •A cross-study consensus set of human organellar marker proteins.
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《Molecular & cellular proteomics : MCP》2020,19(7):1209-1219
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- •In depth performance assessment of leading tools for differential protein abundance.
- •Novel fast modular framework MSqRobSum for robust protein summarization and inference.
- •MsqRobSum outperforms leading protein summarization-based tools.
- •MSqRobSum is on par with top-performing peptide based tool MSqRob.
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Alison M. Kurimchak Vikas Kumar Carlos Herrera-Montvez Katherine J. Johnson Nishi Srivastava Karthik Davarajan Suraj Peri Kathy Q. Cai Gina M. Mantia-Smaldone James S. Duncan 《Molecular & cellular proteomics : MCP》2020,19(12):2068-2090
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- •SRPK1 is overexpressed in endometrial cancer and associated with poor survival.
- •SRPK1 promotes endometrial cancer cell growth under nutrient-deprived conditions.
- •Activation of EGFR-IGF1R-AKT signaling promotes resistance to SRPK1 inhibitors.
- •Co-targeting SRPK1 and EGFR-IGF1R synergize blocking endometrial cancer cell growth.
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《Molecular & cellular proteomics : MCP》2020,19(3):467-477
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- •Each component of the AMPK trimeric complex was profiled by interaction proteomics.
- •The subunit composition of the AMPK complex directs interactions to distinct proteins.
- •AMPK interacts with Artemis and plays a role in Non-Homologous End Joining.
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《Molecular & cellular proteomics : MCP》2020,19(1):1-10
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- •Co-elution stands out as a global interactome mapping method.
- •Benefits include all-to-all protein analysis and measurement of interactome perturbations.
- •Different separation, quantification and bioinformatic strategies are available.
- •Design considerations depend largely on system under study.
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《Molecular & cellular proteomics : MCP》2020,19(5):900-912
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- •Affinity-based proteomics of infected macrophage cells.
- •Salmonella-modified membranes exhibit host-specific composition.
- •Proteome differences explain some host-dependent pathophysiological differences.
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《Molecular & cellular proteomics : MCP》2020,19(7):1132-1144
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- •Proteomes measured from human heart biopsies collected in-vivo covers >7000 cardiac proteins and highlight hundreds of chamber-specific molecular signatures that meaningfully reflect the specialized functions of the respective chambers.
- •Protein quantification from freshly collected biopsies is preferential to necropsy samples because of unspecific post-mortem protein degradation in the latter.
- •Increased abundances of proteins associated with sustained atrial fibrillation are not a sufficient condition to generate the disease state.
- •Protein abundance differences between atria and ventricle primarily originate at the level of gene regulation and reflect a functional need.
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《Molecular & cellular proteomics : MCP》2020,19(2):405-420
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- •Analysis of product ions produced by 213 nm UVPD is used to refine database search.
- •A product ion at the N-terminus of Pro, y-2, is observed in 213 nm UVPD spectra.
- •213 nm UVPD provides more complete proteoform characterization than HCD.
- •HCD and 213 nm UVPD are complementary fragmentation methods for proteoforms <30 kDa.
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《Molecular & cellular proteomics : MCP》2020,19(11):1910-1920
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- •PRMT5 glutathionylation is increased in aged mice or under oxidative stress.
- •Deglutathionylation of PRMT5 is catalyzed by glutaredoxin-1.
- •PRMT5 glutathionylation decreases its methyltransferase activity.
- •PRMT5 glutathionylation results in G2/M arrest and inhibits cell proliferation.
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《Molecular & cellular proteomics : MCP》2020,19(4):690-700
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- •Two molecular groups in anal squamous carcinoma according proteomic profile.
- •Differences in possible targeted processes such as metabolism or immune response.
- •Different percentage of tumor lymphocyte infiltration.
- •Difference in the frequency of ATM variants, related to PPAR inhibitors.
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《Molecular & cellular proteomics : MCP》2020,19(11):1749-1759
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- •In-depth profiling of the serum proteome in early-stage COVID-19 patients.
- •A landscape of inflammation and immune signaling related to the SARS-CoV-2 infection.
- •CCL2 and CXCL10 medicated cytokine signaling pathways may correlate with neutrophil and lymphocyte respectively.
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《Molecular & cellular proteomics : MCP》2020,19(11):1876-1895
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- •Guidelines for studying protein complexes via co-fractionation mass spectrometry.
- •A novel procedure for profiling gold standard protein complexes in CF-MS data.
- •Recommendations for efficient CF-MS fractionation collection.
- •Scoring metric recommendations for precise and sensitive CF-MS data analysis.
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《Molecular & cellular proteomics : MCP》2020,19(4):716-729
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- •Increased proteome coverage with Orbitrap Exploris 480 MS and FAIMS using single compensation voltages and short LC gradients.
- •Towards single-cell proteomics with high-sensitivity analysis of 5 ng HeLa with more than 1,000 proteins identified in 5 minutes using FAIMS and DIA.
- •Deep proteome profiling across twelve rat organs tissues by label-free quantitation using DIA compared to TMT-multiplexing and turboTMT acquisition using phi-SDM.
- •Rapid and sensitive phosphoproteomics with automated enrichment using Ti-IMAC magnetic beads and direct DIA analysis.
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《Molecular & cellular proteomics : MCP》2020,19(1):50-64
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- •BioID reveals the proximity partners of RSK family members.
- •All RSK isoforms associate with and phosphorylate p120ctn on Ser320.
- •RSK negatively regulates adherens junctions and reduces cell-cell adhesion.
- •p120ctn phosphorylation plays a role in the reorganization of proximity partners.