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1.
Hot pepper and tomato fields in the main growing areas in the Rift Valley and the west of Ethiopia were surveyed for virus infections in 1994. A total of 286 samples from hot pepper and 222 samples from tomato plants and associated Datura stramonium L. and Nicandra physalodes Gaertn. weeds with symptoms suggestive of virus infections were collected and analysed using electron microscopy, serology and test plant reactions. Potato virus Y (PVY), Ethiopian pepper mottle virus (EPMV), pepper veinal mottle virus (PVMV) and tomato mosaic virus (ToMV) were detected in hot pepper samples while tomato samples were shown to be infected with tomato mild mottle virus (TMMV), PVY and ToMV. The most widespread and predominant viruses which also occurred frequently in mixed infections were PVY and EPMV in hot pepper and PVY and TMMV in tomato. TMMV was also found in many samples of D. stramonium and N. physalodes. ToMV was identified in only few samples from both crops in the Rift Valley by its characteristic particle morphology, serological properties and symptomatology. PVMV was found in hot pepper samples only from western Ethiopia, but no natural infection of tomato with this virus was revealed. This is the first report on the natural occurrence of TMMV in tomato, D. stramonium and N. physalodes, as well as of ToMV in hot pepper and tomato in Ethiopia. 相似文献
2.
A virus causing ‘eyespot’ leaf symptoms in groundnut plants was transmitted by sap-inoculation and by Aphis craccivora in the non-persistent manner. It infected 16 of 72 species from five of 12 families and was easily propagated in Arachis hypogaea and Physalis floridana. The virus has particles c. 13 × 755 nm and is serologically closely related to soybean mosaic and pepper veinal mottle viruses, and more distantly to four other potyviruses. The virus differs in host range, in vitro properties and serological properties from previously described strains of soybean mosaic and pepper veinal mottle viruses. It seems to be a distinct member of the potyvirus group and we propose the name groundnut eyespot virus. 相似文献
3.
E. Alonso I. García Luque M. J. Avila-Rincón B. Wicke M. T. Serra J. R. Díaz-Ruiz 《Journal of Phytopathology》1989,125(1):67-76
During a four-year (1982–1985) survey of plant viruses infecting pepper cultivars grown under plastic in the Southeastern region of Spain, a tobamovirus was found to be the major disease agent of this crop. The virus produces slight or no symptoms on the leaves, but causes chlorotic mottling, malformation and reduction in size with occasional necrosis on the fruits and was able to infect all commercial pepper cultivars tested, including those resistant to other tobamoviruses, causing a catastrophic disease. The biological and serological characterization of the virus showed that it is very similar to pepper mild mottle virus (PMMV) (Wetter et al. 1984) and therefore we have termed it as Spanish strain of PMMV (PMMV-S). The need of grouping all the so-called “pepper strains” of tobacco mosaic virus (TMV) as a new distinct member of the tobamovirus group with the name of PMMV is emphasized. 相似文献
4.
Bell pepper mottle virus (BPeMV) can be distinguished by symptomatology and host range from other tobamoviruses but a reliable identification needs serological tests. The relationships of BPeMV to tobacco mosaic virus (TMV), Odontoglossum ringspot virus (ORSV), tobacco mild green mosaic virus (TMGMV), and pepper mild mottle virus (PMMV) were investigated using precipitin drop tests on slides, immunodiffusion gel tests, double antibody sandwich enzyme-linked immunosorbent assay (ELISA), and indirectELISA using enzyme-linked goat anti-rabbit globulins for the determination of antiserum titers and serological differentiation indices (SDI). Comparisons of SDIs and amino acid composition data demonstrated that BPeMV is a new species of the tobamovirusgroup. BPeMV, ORSV, PMMV, and TMGMV form a cluster within the genus (group) and could be considered as a subgenus of tobamoviruses. 相似文献
5.
Severe diseases of pepper (Capsicum annuum), tomato (Lycopersicon esculentum), eggplant (Solanum melongena) and tomato eggplant (Solanum integrifolium) in West Africa were induced by pepper veinal mottle virus (PVMV). Five selected virus isolates were serologically similar and readily transmissible by aphids in the non-persistent manner, but they differed in host range and/or symptoms induced in some susceptible species. One isolate from eggplant failed to infect pepper, Chenopodium quinoa and C. amaranticolor, and induced only local infections in tomato. An isolate from tomato failed to infect eggplant, and an isolate from tomato eggplant induced severe stunting in Physalis floridana. The type strain, like the isolate from tomato, failed to infect Nicotiana tabacum systemically, but each caused severe systemic leaf and stem necrosis in tomato. None of the isolates infected S. melongena cv. Long Purple, suggesting that PVMV might be controlled in this and perhaps other crop species by the use of immune or tolerant cultivars. All five isolates were serologically related to potato virus Y and some to six of 12 other potyviruses. 相似文献
6.
7.
A virus isolated from Anagyris foetida and infecting species in the Leguminosae and Solanaceae had typical properties of a tymovirus. It sedimented as two components (49 S and 103 S) and induced the formation of double membrane-bounded invaginations in the chloroplasts of infected cells. Large numbers of ‘empty shells’ were found in the nuclei. The coat protein had a molecular weight of c. 20.000 and the RNA consisted of c. 5500 nucleotides. Crystallization of the virus in laminal crystals could be achieved by precipitation with 10 % polyethylenglycol 6000 and 1 % NaCl. Serologically, the virus was closely related to Scrophularia mottle, Ononis yellow mosaic and Plantago mottle viruses. The four viruses which all infect leguminous hosts are separated by serological differentiation indices which are mostly between 1 and 3. It is therefore suggested that they all should be considered as strains of the same virus which for reasons of priority should have the name Scrophularia mottle virus (ScrMV). The proposed Anagyris strain clearly differs from the Scrophularia mottle, Ononis yellow mosaic and Plantago mottle strains of ScrMV in host range, symptomatology, electrophoretic mobility serological properties and some cytopathogenic effects. It is not clear why the Anagyris strain infects A. foetida systemically in nature, but only locally under greenhouse conditions. 相似文献
8.
A destructive streak disease of tomato (Lycopersicon esculentum) was observed on the University of Ife farm. The disease killed many plants and greatly diminished the quantity and quality of fruit produced by the other plants. A virus that is readily transmitted by mechanical inoculation, by the green peach aphid (Myzus persicae) and by grafting was isolated consistently from diseased plants. The virus was propagated in Nicotiana occidentalis and assayed in Physalis angulata. It was infective after dilution to io-3 but not io-4; after iomin at 55 but not 60oC; or after 5 days but not 7 days at 20–26oC. Electron microscope examination of sap from infected N. occidentalis leaves revealed flexuous rods with a modal length of about 780 nm. Based on the host range and symptomatology, particle morphology and size, properties in vitro and serology, the virus is shown to be related to, and possibly indistinguishable from, pepper veinal mottle virus. 相似文献
9.
10.
Isabel Aguilar Flora Sánchez Africa Martín Martín David Martínez-Herrera Fernando Ponz 《Plant molecular biology》1996,30(1):191-197
The complete nucleotide sequence of Chinese rape mosaic virus has been determined. The virus is a member of the tobamovirus genus of plant virus and is able to infect Arabidopsis thaliana (L.) Heynh systemically. The analysis of the sequence shows a gene array that seems to be characteristic of crucifer tobamoviruses and which is slightly different from the one most frequently found in tobamoviruses. Based on gene organization and on comparisons of sequence homologies between members of the tobamoviruses, a clustering of crucifer tobamoviruses is proposed that groups the presently known crucifer tobamovirus into two viruses with two strains each. A name change of Chinese rape mosaic virus to oilseed rape mosaic virus is proposed.Abbreviations 2-ME
2-mercaptoethanol
- EDTA
ethylenediaminetetraacetic acid
- SDS
sodium dodecyl sulfate
- UTR
untranslated region
- MP
movement protein
- CP
capsid protein
- CRMV
Chinese rape mosaic virus
- TVCV
turnip vein clearing virus
- PaMMV
paprika mild mottle virus
- PMMV-I
pepper mild mottle virus (Italian isolate)
- PMMV-S
pepper mild mottle virus (Spanish isolate)
- ToMV
tomato mosaic virus
- TMV
tobacco mosaic virus
- TMGMV
tobacco mild green mosaic virus
- ORSV
odontoglossum ringspot virus
- SHMV
sunn hemp mosaic virus
- CGMMV
cucumber green mottle mosaic virus
- ORMV
oilseed rape mosaic virus 相似文献
11.
A.I. Bhat A. Siljo S. Devasahayam 《Archives Of Phytopathology And Plant Protection》2013,46(9):1000-1009
Symptomless nature of Piper yellow mottle virus (PYMoV) infection in three varieties of black pepper (Piper nigrum) (Panniyur 1, Panniyur 5 and Panchami) and a wild species of Piper (Piper colubrinum) was confirmed by polymerase chain reaction (PCR) using PYMoV specific primers. The virus could be transmitted from these PYMoV-infected symptomless plants onto symptom producing black pepper cv. Karimunda through mealybug vector, Ferrisia virgata and by graft transmission. About 20–50% seedlings showed typical symptoms of the PYMoV at 30 days after mealybug inoculations while it was 75–94% at 90 days after inoculation. PCR test of the inoculated seedlings confirmed the presence of PYMoV in 50–64%, 76–100% and 80–100% of plants in 30, 60 and 90 days after inoculation, respectively. Similarly, 50–66%, 91–100% and 100% of graft-transmitted plants showed typical symptoms of the disease at 30, 60 and 90 days after grafting. PCR test of the graft-transmitted plants showed 100% PYMoV infection at 60 days after grafting. The results clearly demonstrated the existence of PYMoV-infected symptomless plants that can act as source for secondary spread of the virus in the field. 相似文献
12.
R. D. PARES 《The Annals of applied biology》1988,112(3):609-612
A New South Wales isolate (Ca) of capsicum mosaic virus was tested against antisera to it and capsicum tobamovirus isolates from the Netherlands (P8, P11), USA (SL), Argentina (FO) and Sicily (PM). The comparison demonstrated that the four viruses Ca, P8, PM and SL are closely related to each other, forming a series of decreasing relationship to Ca in the above order. FO was related to these but insufficiently to be considered part of the group, and P11 was only slightly related to the others. The literature on serology of tobamoviruses in Capsicum spp. was collated and it is suggested that isolates from Sicily (pepper mild mottle), Australia (capsicum mosaic), The Netherlands (P8, P14) and USA (SL) be considered as strains of a virus distinct from both tobacco mosaic and tomato mosaic viruses and that these isolates all be referred to in future as strains of pepper mild mottle virus. 相似文献
13.
Dr. J. L. Ladipo Dr. D.-E. Lesemann Dr. Renate Koenig 《Journal of Phytopathology》1988,122(4):359-371
Two potyvirus isolates from the eggplant (Solanum melongena L.) cultivars ‘Ex Benin’ and ‘Ex Jos’, respectively, in Nigeria proved to be almost identical in host range, symptomatology and reactivity with antisera to various potyviruses. In eggplant they caused a severe systemic mottle, blistering and malformation of leaves and an abnormal serration of the leaf margins. A potyvirus isolate from tomato showing mosaic symptoms was similar, but not identical to the eggplant isolates. In the slide, precipitin test the serological differentiation indices were between 1 and 3 for the eggplant and tomato isolates. In the immunoelectron microscopical decoration test all three virus isolates showed some reactivity with antisera to the following potyvir, uses: dioscorea green banding mosaic, groundnut eyespot, a mungbean isolate of peanut stripe, pepper veinal mottle, telfairia mosaic and a tomato isolate from Taiwan. No reactions were observed with antisera to other potyviruses. Cytopathogenic effects w,ere similar for all three isolates in the arrangement of virus particles, the structure of the cylindrical inclusions and the occurrence of clusters of small vesicles. However, other cytological alterations like accumulations of rod-shaped aggregates of,granular material, formation of giant mitochondria, degeneration of mitochondria and occurrence of a nucleolar inclusion differentiated the isolates. 相似文献
14.
Transgenic pepper plants coexpressing coat proteins (CPs) of cucumber mosaic virus (CMV-Kor) and tomato mosaic virus (ToMV) were produced by Agrobacterium-mediated transformation. To facilitate selection for positive transformants in transgenic peppers carrying an L gene, we developed a simple and effective screening procedure using hypersensitive response upon ToMV challenge inoculation. In this procedure, positive transformants could be clearly differentiated from the nontransformed plants. Transgenic pepper plants expressing the CP genes of both viruses were tested for resistance against CMV-Kor and pepper mild mottle virus (PMMV). In most transgenic plants, viral propagation was substantially retarded when compared to the nontransgenic plants. These experiments demonstrate that our transgenic pepper plants might be a useful marker system for the transgene screening and useful for classical breeding programs of developing virus resistant hot pepper plants. 相似文献
15.
Manuel G. Moreno-Pérez Sayanta Bera Michael McLeish Aurora Fraile Fernando García-Arenal 《Molecular Plant Pathology》2023,24(2):142-153
An instance of host range evolution relevant to plant virus disease control is resistance breaking. Resistance breaking can be hindered by across-host fitness trade-offs generated by negative effects of resistance-breaking mutations on the virus fitness in susceptible hosts. Different mutations in pepper mild mottle virus (PMMoV) coat protein result in the breaking in pepper plants of the resistance determined by the L3 resistance allele. Of these, mutation M138N is widespread in PMMoV populations, despite associated fitness penalties in within-host multiplication and survival. The stability of mutation M138N was analysed by serial passaging in L3 resistant plants. Appearance on passaging of necrotic local lesions (NLL), indicating an effective L3 resistance, showed reversion to nonresistance-breaking phenotypes was common. Most revertant genotypes had the mutation N138K, which affects the properties of the virus particle, introducing a penalty of reversion. Hence, the costs of reversion may determine the evolution of resistance-breaking in addition to resistance-breaking costs. The genetic diversity of the virus population in NLL was much higher than in systemically infected tissues, and included mutations reported to break L3 resistance other than M138N. Infectivity assays on pepper genotypes with different L alleles showed high phenotypic diversity in respect to L alleles in NLL, including phenotypes not reported in nature. Thus, high diversity at NLL may potentiate the appearance of genotypes that enable the colonization of new host genotypes or species. Collectively, the results of this study contribute to better understanding the evolutionary dynamics of resistance breaking and host-range expansions. 相似文献
16.
A new virus, peanut stripe (PStV), isolated from groundnut (Arachis hypogaea) in the USA, induced characteristic striping, discontinuous vein banding along the lateral veins, and oakleaf mosaic in groundnut. The virus was also isolated from germplasm lines introduced from the People's Republic of China. PStV was transmitted by inoculation of sap to nine species of the Chenopodiaceae, Leguminosae, and Solanaceae; Chenopodium amaranticolor was a good local lesion host. PStV was also transmitted by Aphis craccivora in a non-persistent manner and through seed of groundnut up to 37%. The virus remained infective in buffered plant extracts after diluting to 10-3, storage for 3 days at 20°C, and heating for 10 min at 60°C but not 65°C. Purified virus preparations contained flexuous filamentous particles c. 752 nm long, which contained a major polypeptide of 33 500 daltons and one nucleic acid species of 3·1 × 106 daltons. In ELISA, PStV was serologically related to blackeye cowpea mosaic, soybean mosaic, clover yellow vein, and pepper veinal mottle viruses but not to peanut mottle, potato Y, tobacco etch, and peanut green mosaic viruses. On the basis of these properties PStV is identified as a new potyvirus in groundnut. 相似文献
17.
Plantago mottle virus (RMV), a member of the tymovirus group, was identified as the causal agent of a disease of pea (Pisum sativum) in New York State. The pea virus isolates were identical in host range and serology to the type strain from Plantago major. In susceptible pea genotypes symptoms were strongly influenced by ambient temperature; high temperature (35°C) reduced infectivity and suppressed symptoms, whereas low temperature (15 and 25°C) prolonged the incubation period but favoured the development of conspicuous leaf veinal chlorosis, mottle and necrosis. Resistance to P1MV was found in seventeen of twenty-five domestic pea cultivars and in two of twelve foreign introductions. Many of the P1MV-resistant lines were resistant also to bean yellow mosaic virus. The use of resistant cultivars and the apparent limited conditions for efficient transmission of this virus have minimized its importance to pea crops in New York State. 相似文献
18.
Carnation vein mottle virus (CarVMV) is rare in glasshouse carnations in Britain, although locally common in Dianthus barbatus in private gardens. In Sim carnations free from other viruses, CarVMV caused slight diffuse chlorotic mottling in the younger leaves, decreased flower yield by c. 22%, and caused flower breaking in cvs William Sim and Dusty. In non-Sim cultivars Pink Shibiuya, Orchid Beauty and Vesta, leaf symptoms and flower breaking were more pronounced. In mixed infections with carnation mottle virus, symptoms were much more severe. CarVMV was not eliminated from carnation or D. barbatus plants grown for 4 wk at 37oC, and only rarely from cuttings then taken from them, but it was readily eliminated by meristem-tip culture. Myzus persicae adults or nymphs acquired and transmitted the virus within a total time of 4 min, and remained infective for 30–60 min if feeding, or for 75 min if starved. The carnation aphid, M. persicae f. dianthi, transmitted the virus much less efficiently. The virus was not transmitted by dodder (Cuscuta campestris), or through seed of D. barbatus or Chenopodium quinoa. The maximum infective dilution in sap of D. barbatus, carnation and C. quinoa ranged from 10-2 to 10-5. The virus withstood 10 min at 60 but not 65oC, up to 9 days at c. 18oC or 3–4 wk at c. 2oC. CarVMV infected twenty-two of 107 plant species in six of thirty-seven families; suscepts were confined to the Chenopodiaceae, Caryophyllaceae and closely allied families. C. quinoa was the best local lesion assay host. Seedling clones of D. barbatus, selected as resistant to carnation mottle virus, proved the best indicator and propagation species. Up to 50 mg virus/kg tissue were obtained by butanol clarification followed by differential and density gradient centrifugation. The preparations contained a single sedimenting component, s20w= 144S, and had flexuous filamentous particles, c. 790 times 12 run; the particles contained a single polypeptide, mol. wt 34800, and 5% of a single-stranded ribonucleic acid (RNA) with nucleotide base ratios of G21: A25: C25: U29. Serologically CarVMV was related distantly to turnip mosaic (cabbage black ring strain), pea mosaic, watermelon mosaic (Strain 2) and bean yellow mosaic viruses, more closely to pepper veinal mottle virus, but unrelated to twelve other potyviruses. CarVMV is not at present a danger to carnation crops in Britain, but the recent trend of sending carnation plants to overwinter outdoors in warmer countries involves potential risks of more rapid spread by effective vector races of M. persicae. 相似文献
19.
OLWEN M. STONE 《The Annals of applied biology》1968,62(1):119-122
Carnation mottle virus (CarMV), carnation ringspot virus (CRSV), carnation vein mottle virus (CarVMV) and carnation latent virus (CarLV) were all eliminated from both carnation (Dianthus caryophyllus) and sweet william (D. barbatus) plants by meristem-tip culture. Carnation mottle virus was more readily eliminated from D. barbatus than from carnation. Carnation vein mottle and carnation latent viruses were more readily eliminated from carnation than from sweet william: they are rarely found in carnation but CarVMV is found frequently in sweet william. Carnation ringspot was eliminated equally readily from both hosts. 相似文献