共查询到20条相似文献,搜索用时 15 毫秒
1.
M. JAAROLA M. RATKIEWICZ R. T. ASHFORD C. BRUNHOFF A. BORKOWSKA 《Molecular ecology resources》2007,7(6):1029-1031
We developed nine polymorphic microsatellite loci for the field vole, Microtus agrestis. The number of alleles ranged from five to 15 and observed heterozygosities ranged from 0.40 to 1.00. We also tested the microsatellite loci for amplification and polymorphism in the congeneric species Microtus arvalis. Five of the nine loci were successfully analysed in this species. The microsatellite markers will be employed in studies of reproductive success and fine‐scale spatial genetic structure. 相似文献
2.
Angélica Cibrián-Jaramillo Thomas E. Marler Rob DeSalle Eric D. Brenner 《Conservation Genetics》2008,9(4):1051-1054
We developed a set of 12 EST-microsatellite markers (EST-STRs) from Cycas rumphii Miq. and tested them on an ex situ collection of the endangered Cycas micronesica K.D. Hill. The number of alleles per locus in both species ranged from 1 to 15. Observed and expected heterozygosities ranged
from 0.000 to 0.842, and 0.000 to 0.883, respectively. All primers amplified in four additional Cycas species. These markers are the first genome-enabled tools for cycad population level studies, and are now available to inform
conservation efforts and disentangle the biogeographic history of the genus. 相似文献
3.
HALVOR KNUTSEN DAVID FIANI HANNE SANNS A. RUS HOELZEL 《Molecular ecology resources》2007,7(5):851-853
We developed polymerase chain reaction primers for nine dinucleotide microsatellite loci in the marine deep‐sea fish, the tusk (Brosme brosme). All markers were obtained from a partial genomic DNA library, and characterized in 100 unrelated individuals from one putative population. The number of alleles ranged from two to 60 with an average of 15/locus. The observed heterozygosity ranged from 0.020 to 0.826 (average 0.438). Several of the markers amplified multiple alleles from the two other gadoid species tested. 相似文献
4.
We report the isolation and characterization of 19 polymorphic microsatellite DNA markers in the Japanese brown frog (Rana japonica). These markers were tested in 24 individuals each collected from three distinct populations in Ichikai-machi, Tochigi Prefecture. The number of observed alleles per locus ranged from 3 to 24 across all populations, and the values of observed and expected heterozygosities ranged from 0.130 to 1 and from 0.125 to 0.941, respectively. These microsatellite loci will be useful for investigating the intraspecific genetic variation and population structure of this species. 相似文献
5.
Twelve polymorphic microsatellite loci were isolated and characterized from the threatened toad lily Tricyrtis flava. The number of alleles ranged from three to 17, and the observed and expected heterozygosities ranged from 0.35 to 0.96 and from 0.50 to 0.93, respectively, in a population of T. flava. We also tested cross‐species amplifications of the markers in three other species of Trycyrtis sect. Flavae. Most of 12 markers developed for T. flava were successfully amplified in the species of sect. Flavae and will be useful for population genetic studies of these species. 相似文献
6.
We characterized 15 polymorphic tetranucleotide microsatellite markers for the ringtail, Bassariscus astutus. We tested these loci in 21 individuals captured in Arizona and Texas and found six to 19 alleles per locus. Observed and expected heterozygosities ranged from 0.381 to 1.000 and from 0.381 to 0.941, respectively. All loci were in Hardy-Weinberg equilibrium, and none were in linkage disequilibrium. These markers may be used to investigate population genetics and mating patterns in this species. 相似文献
7.
INGA ZEISSET THOMAS DAMM ALS JOSEF SETTELE JACOBUS J. BOOMSMA 《Molecular ecology resources》2005,5(1):165-168
We developed microsatellite markers for Maculinea nausithous and Maculinea alcon, two of five species of endangered large blue butterflies found in Europe. Two separate microsatellite libraries were constructed. Eleven markers were developed for M. nausithous and one for M. alcon. The primers were tested on both species as well as on the three other European Maculinea species. The number of alleles per locus ranged from two to 14. These markers will be useful tools for population genetic studies of Maculinea species. 相似文献
8.
We report on the characterization of 11 polymorphic microsatellite loci in P. viridis, the first set of such markers developed and characterized for this species. The number of alleles per locus ranged from 2 to 7, whereas the observed heterozygosity ranged from 0.0447 to 0.4837. These markers should prove useful as powerful genetic markers for this species. 相似文献
9.
Thirty-four novel microsatellite markers developed for wood cricket (Nemobius sylvestris) were tested and optimized. Twenty-five microsatellite loci were polymorphic, exhibiting between two and nine alleles. Observed heterozygosities ranged from 0.038 to 0.925. The microsatellites were also tested in a species belonging to another genus of the Gryllidae family (Gryllus bimaculatus). Two markers produced clear banding patterns with the expected product size. These markers will be used to study the effects of forest fragmentation on genetic connectivity using wood cricket as a model species. 相似文献
10.
We have isolated and characterized twelve novel polymorphic microsatellite loci from Scapharca subcrenata to analyse the population structure. The number of observed alleles per locus ranged from 3 to 17. Observed heterozygosity
(H
O) ranged from 0.321 to 0.929. Cross-species amplification was tested successfully in three other bivalve species. These microsatellite
markers will be useful for genetic diversity studies of S. subcrenata and other Lamellibranchia species. 相似文献
11.
Panyu Hua Tingting Guo Wenchao Liu Shuyi Zhang Stephen J. Rossiter 《Conservation Genetics》2009,10(3):597-600
We used the enriched genomic library method to isolate and characterize dinucleotide microsatellite loci in the least horseshoe
bat, Rhinolophus pusillus. Seventeen loci were obtained and tested on 31 individuals sampled from Guangxi Province in southern China. Thirteen of these
markers were polymorphic with expected heterozygosity ranging from 0.821 to 0.909. A total of 164 alleles were detected and
the number of alleles per locus ranged from 9 to 16 (mean 12.6). These polymorphic markers will be used to assess population
structure in R. pusillus. In addition, successful cross-amplification in five congeneric bat species suggests most of these markers will also be useful
for studying related species. 相似文献
12.
Hugo Le Chevalier Neus Marí‐Mena Beln Carro Jrme G. Prunier Coralie Bossu Elodie Darnet Jrmie Souchet Olivier Guillaume Olivier Calvez Romain Bertrand Laurent Barthe Gilles Pottier Albert Martínez‐Sylvestre Isabel Verdaguer‐Foz Marc Mossoll‐Torres Audrey Trochet Fabien Aubret 《Ecology and evolution》2019,9(19):11227-11231
Nineteen polymorphic microsatellite loci were identified and developed for Natrix maura. Polymorphism was assessed for 120 individuals sampled across four sampling sites from the French Pyrenees Mountains. The number of alleles per locus ranged from 3 to 15, and expected heterozygosity per locus ranged from 0.227 to 0.863. We tested for deviation from Hardy–Weinberg equilibrium and linkage disequilibrium and assessed the presence of null alleles for all loci, resulting in a selection of 14 high‐quality polymorphic markers. These markers will be extremely useful in identifying fine‐scale genetic structures and providing insight into conservation management plans of this species. 相似文献
13.
We report ten microsatellite loci in the Emei moustache toads, Leptobrachium boringii. Markers were obtained by screening a genomic library enriched for microsatellite motifs. Twenty-four individuals from one
breeding site were examined and ten loci were polymorphic. The number of alleles per locus ranged from 3–9 with an average
of 6.3/locus. The expected heterozygosity and observed heterozygosity ranged from 0.3874 to 0.8432, and from 0.4583 to 0.9167,
respectively. Cross-species amplification was tested in a closely related species L. leishanensis. These markers will be useful in future studies on characterizing the mating system of the species. 相似文献
14.
We report on the characterization of 11 polymorphic microsatellite loci in P. viridis, the first set of such markers developed and characterized for this species. The number of alleles per locus ranged from
2 to 7, whereas the observed heterozygosity ranged from 0.0447 to 0.4837. These markers should prove useful as powerful genetic
markers for this species.
This article was submitted by the authors in English. 相似文献
15.
We developed primers for two dinucleotide and eight tetranucleotide microsatellite loci in a marine fish, the ling (Molva molva). All markers were obtained from partial genomic DNA libraries and characterized in 55 unrelated individuals from one putative population. The number of alleles ranged from five to 24 (average 10.5) per locus, and the observed heterozygosity ranged from 0.218 to 0.981 (average 0.643). No loci amplified in two other gadoid species tested, the Atlantic cod (Gadus morhua) and the tusk (Brosme brosme). 相似文献
16.
Kautz S Schmid VS Trindl A Heinze J Ballhorn DJ Heil M 《Molecular ecology resources》2009,9(3):1016-1019
To investigate the population structure of the obligate plant-ant Pseudomyrmex ferrugineus, we developed primers for 12 microsatellite loci. We tested the variability of the markers on 11 individuals from each of two populations (totalling 22 individuals) and found two to 12 alleles per locus and population. No deviations from Hardy-Weinberg equilibrium were detected. Observed and expected heterozygosities at each locus ranged from 0.00 to 0.50 and from 0.08 to 0.46, respectively. We also investigated suitability of these primers in two congeneric species. 相似文献
17.
We report the isolation of five microsatellites loci from the sugar beet cyst nematode (Heterodera schachtii). Multilocus genotypes were obtained on individual larvae freshly emerged from cysts. Allelic diversity ranged from four to 27 among the five loci. The primers were tested for cross‐species amplification in six other species of phytoparasitic nematodes of the Heterodera genus. Those molecular markers will be used to study the genetic structure of this obligatory parasite and how it is affected by the use of resistant plants. 相似文献
18.
I. L. G. NURTJAHJANINGSIH Y. SAITO C. L. LIAN Y. TSUDA Y. IDE 《Molecular ecology resources》2005,5(3):552-553
Pinus merkusii is an important industrial species that is distributed only in Southeast Asia. We isolated 10 microsatellite markers from this species using a dual‐suppression‐polymerase chain reaction technique. Of these markers, five loci were codominant and polymorphic. The number of alleles per locus ranged from three to six and the expected heterozygosity ranged from 0.389 to 0.728. These microsatellite markers will be available for analysis on population genetics and mating patterns. 相似文献
19.
We developed and tested microsatellite markers to investigate population structure of a threatened North American freshwater gastropod, Taylorconcha serpenticola. Of the 21 primer pairs that were evaluated, 11 were readily optimized and scored, providing amplification of 12 loci that were screened for 820 specimens from 29 populations. The number of alleles across 11 of these polymorphic loci ranged from three to 20 and the observed heterozygosity varied from 0.0061 to 0.7561. All loci yielded suitable amplification products in the second species of Taylorconcha (T. insperata) and three proved to be diagnostic for these congeners, demonstrating that these markers are also useful for species identification studies. 相似文献
20.
Ramesh K. Aggarwal Albert Lalremruata Thirumalaisamy P. Velavan Ayyadevara Pavani Sowjanya Lalji Singh 《Conservation Genetics》2008,9(4):981-984
Olive ridleys, one of the widely distributed marine turtle species has undergone declines in recent years due to multiple
anthropogenic factors warranting conservation efforts for which assessment of genetic variability in existing populations
become critical. Here we describe development of ten new microsatellite markers from a short sequence repeat-enriched partial
genomic DNA library, which are found to be highly informative for genetic studies. Eight of these markers when tested on 83
olive ridley turtles revealed high allelic diversity (4–27 alleles per marker), and high observed and expected heterozygosity
estimates that ranged from 0.29 to 0.82 and 0.62 to 0.94, respectively. Two microsatellites were monomorphic in the tested
olive ridley samples, but were found to be informative/polymorphic when tested on related marine turtle species. More importantly,
nine of the new markers showed robust cross-species amplifications in three related species Dermochelys coriacea, Chelonia mydas and Eretmochelys imbricata. Thus, this study describes ten new microsatellite markers and also demonstrates their potential as efficient genetic markers
in studies related to parentage analysis, population structure, phylogeography and species relationships of olive ridleys
and other marine turtle species. 相似文献