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1.
Laura E. R. Martin Andrew W. Byrne James O’Keeffe Michele A. Miller Francisco J. Olea-Popelka 《European Journal of Wildlife Research》2017,63(1):30
European badgers (Meles meles) in Ireland and the UK are a reservoir for Mycobacterium bovis, the causative agent of bovine tuberculosis (TB). A number of interventions have been evaluated in attempts to control bovine TB within badger populations, and many of which rely on the capture of badgers. One strategy being implemented within Ireland is intramuscular vaccination using Bacillus Calmette-Guérin (BCG), as an alternative to badger culling. The success of vaccination as a disease control strategy depends on the ability to capture badgers and administer vaccines; thus, trapping success is crucial to effectively vaccinate the population (maximize vaccine coverage). A field vaccine trial was conducted in County Kilkenny, Ireland, from 2010–2013. We used data from this trial to evaluate the association between weather (precipitation and temperature data), badger sett characteristics, and badger trapping success. Approximately 10% of capture efforts resulted in a badger capture. Our results indicate that badger captures were the highest in drizzle, rain, and heavy rain weather conditions, and when minimum temperatures ranged from 3–8 °C. Badger captures were the highest at main setts (large burrow systems), and when sett activity scores were high (qualitative classes 4 or 5). Using local precipitation and temperature data in conjunction with observed sett characteristics provides wildlife managers with guidelines to optimize trapping success. Implementing capture operations under optimal conditions should increase the trapping success of badgers and allow for increased delivery of vaccines to manage bovine TB. 相似文献
2.
Background
Salmonella spp. have been isolated from a wide range of wild animals. Opportunistic wild carnivores such as red foxes (Vulpes vulpes) and badgers (Meles meles) may act as environmental indicators or as potential sources of salmonellosis in humans. The present study characterizes Salmonella spp. isolated from the intestinal contents of hunted or dead red foxes (n?=?509) and badgers (n?=?17) in northern Italy.Findings
Thirty-one strains of Salmonella belonging to 3 Salmonella enterica subspecies were isolated. Fourteen different serovars of S. enterica subsp. enterica were identified, among which were serovars often associated with human illness.Conclusions
Wild opportunistic predators can influence the probability of infection of both domestic animals and humans through active shedding of the pathogen to the environment. The epidemiological role of wild carnivores in the spread of salmonellosis needs to be further studied.3.
Emmanuel Do Linh San Nicola Ferrari Jean-Marc Weber 《European Journal of Wildlife Research》2007,53(4):265-275
This study reports for the first time data on the spatio-temporal ecology of badgers living in a cold and wet mountain region
(Swiss Jura Mountains). The home range, movements, activity patterns and habitat use of three badgers (two males, one female)
were examined using radiotelemetry. Average home range size was 320 ha (MCP 100%), but the ranging behaviour of badgers varied
at a seasonal scale. As in other regions, badgers were strictly nocturnal or crepuscular and showed a marked reduction of
activity in the winter period. From spring to autumn, animals were active for an average (±SD) of 8.1 ± 2.4 h and travelled
up to 9,460 m each night (mean±SD, 5,160 ± 2,600 m). The nightly distance travelled by badgers was positively correlated with
their travel speed, the duration of the activity period and the used area, but not with night length. Year-round, the radio-collared
animals avoided pastures and the vicinity of houses during their night trips. In winter and spring, individual badgers used
forests and wooded pastures more than expected according to their availability, whereas cereal fields were actively selected
in summer and autumn. Den-watching, night-lighting and radio-tracking data suggest that badgers live in pairs in this wet
and cold region. Population density estimates range from 0.4 to 1.5 individuals/100 ha. We discuss the importance of trophic
resources and climate as factors influencing badger behavioural ecology. 相似文献
4.
Filipa Loureiro Luís Miguel Rosalino David W. Macdonald Margarida Santos-Reis 《Ecological Research》2007,22(5):837-844
Movement is the process by which individual organisms are displaced over time to eat, reproduce and defend resources. Fractal
analysis is a technique used to study animal movement that measures spatial complexity of path tortuosity; here, we apply
it to characterize the movement patterns of the Eurasian badger (Meles meles) in a Mediterranean landscape. We calculated path tortuosity overall and seasonally, and for individuals of different sexes
and social groups. The influence of variables related to badgers’ resources (food, shelter, water), human infra-structures
and weather conditions were analysed with respect to the tortuosity of each badger’s path. A total of 55 search paths from
six badgers were considered for this study. Although badgers generally displayed convoluted movement, there were two exceptions:
(a) males overall and (b) all badgers in summer; for both, movements had a lower fractal value, i.e. were less tortuous. The
convoluted movement pattern generally observed is probably adapted to the clumped distribution of food in the study area.
Nevertheless, our results suggest that the use of dens and latrines were the principal determinants of tortuosity of badgers’
paths while foraging. 相似文献
5.
D. P. Sleeman J. Davenport S. J. More T. A. Clegg J. M. Griffin I. O’Boyle 《European Journal of Wildlife Research》2009,55(3):267-278
This study’s objective was to estimate the permeability of barriers to badger immigration during the Irish Four Area project.
These barriers were at the boundaries of removal areas, where there was proactive culling of badgers. Data from the last 3 years
of the study were used. Each length of barrier was allocated a space within the removal area. These were further sub-divided
into spaces of 0–2, 2–5 km and sometimes of more than 5 km from the edge of the removal area. It is assumed that all, or some,
of the badgers caught within these spaces came across the barriers. The barriers were one of the following: external buffers,
sea, rivers and political boundaries. The total lengths of the barriers in all areas were: external buffer 128.5 km; sea 70.9 km;
river 78.6 km; political 32.2 km. We assume three scenarios: (1) all badgers caught in the final 3 years were immigrants,
(2) 75% were immigrants or (3) 50% were immigrants. We test these scenarios using chi-square tests, applying internal buffers
of 1 km to counter movements of badgers across zones. Using this approach and multivariate analysis, we found that the permeability
of barrier types varied, with sea and external buffers being the most effective barriers. The combined capture data are further
examined by the sex ratio in each range, and then the sex ratio in total. Equal numbers of males and females were found, but
the source populations were probably predominantly female. If badger management options are to achieve maximum benefits, then
the field effectiveness of such barriers needs to be understood. 相似文献
6.
7.
Noel H. Holmgren 《Brittonia》2018,70(1):115-139
A revision of Penstemon sect. Saccanthera subsect. Serrulati includes a new species (P. salmonensis), a new variety (P. triphyllus var. infernalis), and the elevation of a subspecies to species (P. curtiflorus), bringing the total number of species to eight, which are keyed and described, complete with nomenclature and type citations. 相似文献
8.
Morten Elmeros Dorthe Malene Götz Mikkelsen Louise Solveig Nørgaard Cino Pertoldi Trine Hammer Jensen Mariann Chriél 《Acta theriologica》2018,63(4):405-413
The raccoon dog (Nyctereutes procyonoides) is an East Asian Canid that has been introduced in Europe. Introduction of alien species is an increasing conservation issue. We examined the diet of a recently established raccoon dog population in Denmark by analysing stomach content in 249 carcasses collected in 2008–2016. Raccoon dog diet was compared to the diet of native badger (Meles meles) and red fox (Vulpes vulpes) in Denmark. The most common food for raccoon dogs were invertebrates (frequency of occurrence, FO 69%), small mammals (FO 68%), birds (FO 41%), fruits (FO 38%), amphibians (FO 36%) and carrions (FO 34%). The occurrence of invertebrates was highest during spring and summer, while fruits, cereals and carrions were eaten most often during autumn and winter. As expected, raccoon dog shared the major food categories with badger and red fox, but generally, it had a wider dietary niche. Overall, dietary overlap between raccoon dog and badger was 0.74 (Pianka index, Ojk). The dietary overlap with red fox was relatively high in all seasons, peaking in summer (Ojk 0.87) and dropping in winter (Ojk 0.79). Despite the dietary overlap between the alien racoon dog and native red fox and badger, the species may coexist due to partitioning of feeding habitats and/or because the red fox is limited by other factors, e.g. diseases and anthropogenic activities. The introduced raccoon dog seems to fit a dietary niche between badger and red foxes in human-dominated landscapes in north-western Europe. 相似文献
9.
Two repeated DNA sequences isolated from a partial genomic DNA library of Helianthus annuus, p HaS13 and p HaS211, were shown to represent portions of the int gene of a Ty3 /gypsy retroelement and of the RNase-Hgene of a Ty1 /copia retroelement, respectively. Southern blotting patterns obtained by hybridizing the two probes to BglII- or DraI-digested genomic DNA from different Helianthus species showed p HaS13 and p HaS211 were parts of dispersed repeats at least 8 and 7 kb in length, respectively, that were conserved in all species studied. Comparable hybridization patterns were obtained in all species with p HaS13. By contrast, the patterns obtained by hybridizing p HaS211 clearly differentiated annual species from perennials. The frequencies of p HaS13- and p HaS211-related sequences in different species were 4.3x10(4)-1.3x10(5) copies and 9.9x10(2)-8.1x10(3) copies per picogram of DNA, respectively. The frequency of p HaS13-related sequences varied widely within annual species, while no significant difference was observed among perennial species. Conversely, the frequency variation of p HaS211-related sequences was as large within annual species as within perennials. Sequences of both families were found to be dispersed along the length of all chromosomes in all species studied. However, Ty3 /gypsy-like sequences were localized preferentially at the centromeric regions, whereas Ty1/ copia-like sequences were less represented or absent around the centromeres and plentiful at the chromosome ends. These findings suggest that the two sequence families played a role in Helianthusgenome evolution and species divergence, evolved independently in the same genomic backgrounds and in annual or perennial species, and acquired different possible functions in the host genomes. 相似文献
10.
A genetic transformation system has been developed for callus cells of Crataegus
aronia using Agrobacterium
tumefaciens. Callus culture was established from internodal stem segments incubated on Murashige and Skoog (MS) medium supplemented with
5 mg l−1 Indole-3-butyric acid (IBA) and 0.5 mg l−1 6-benzyladenine (BA). In order to optimize the callus culture system with respect to callus growth and coloration, different
types and concentrations of plant growth regulators were tested. Results indicated that the best average fresh weight of red
colored callus was obtained on MS medium supplemented with 2 mg l−1 2,4-dichlorophenoxyacetic acid (2,4-D) and 1.5 mg l−1 kinetin (Kin) (callus maintenance medium). Callus cells were co-cultivated with Agrobacterium harboring the binary plasmid pCAMBIA1302 carrying the mgfp5 and hygromycin phosphotransferase (hptII) genes conferring green fluorescent protein (GFP) activity and hygromycin resistance, respectively. Putative transgenic calli
were obtained 4 weeks after incubation of the co-cultivated explants onto maintenance medium supplemented with 50 mg l−1 hygromycin. Molecular analysis confirmed the integration of the transgenes in transformed callus. To our knowledge, this
is the first time to report an Agrobacterium-mediated transformation system in Crataegus
aronia. 相似文献
11.
Studying Pneumocystis has proven to be a challenge from the perspective of propagating a significant amount of the pathogen in a facile manner.
The study of several fungal pathogens has been aided by the use of invertebrate model hosts. Our efforts to infect the invertebrate
larvae Galleria
mellonella with Pneumocystis proved futile since P. murina neither caused disease nor was able to proliferate within G. mellonella. It did, however, show that the pathogen could be rapidly cleared from the host. 相似文献
12.
13.
We have analyzed the expression patterns of two Fox genes, FoxE and FoxQ, in the ascidian Ciona intestinalis. Expression of Ci-FoxE was specific to the endostyle of adults, being prominent in the thyroid-equivalent region of zone 7. Ci-FoxQ was expressed in several endodermal organs of adult ascidians, such as the endostyle, branchial sac and esophagus. In the endostyle, the pattern of Ci-FoxQ expression was similar to that of CiTTF-1, being prominent in the thyroid-equivalent regions of zones 7 and 8. Therefore, these Fox genes may perform thyroid-equivalent functions in the ascidian endostyle.Edited by N. Satoh 相似文献
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15.
David J. Kelly Leigh A. L. Corner Eamonn Gormley Denise Murphy Eamon Costello Frank E. Aldwell Nicola M. Marples 《European Journal of Wildlife Research》2011,57(4):767-774
European badgers (Meles meles) are a wildlife reservoir for Mycobacterium bovis infection (tuberculosis) in Ireland and the UK and are implicated in the transmission of infection to livestock. Vaccination
of badgers with the human BCG vaccine (Bacille Calmette Guerin) is considered as an important strategy to reduce the burden
of disease in this species, and a pragmatic approach is likely to involve oral vaccination. In this study, we evaluated nine
different flavours for use as attractants in a prototype oral vaccine bait for European badgers (M. meles): aniseed, apple, cocoa powder, carob powder, curry, fish, garlic, peanut and strawberry. The bait matrix was composed of
a natural lipid formulation, developed as a vehicle for oral vaccination against tuberculosis in wildlife. A ‘food for work’
paradigm was employed during the trials to ensure the animals were actively seeking the baits. The trials showed carob and
cocoa powders were equally attractive and more attractive than any of the other candidates. Carob and cocoa show potential
as bait attractants for badgers and might form part of a novel vaccine delivery system. 相似文献
16.
We have identified a mutant slowmotion phenotype in first instar larval peristaltic behaviour of Drosophila. By the end of embryogenesis and during early first instar phases, slowmo mutant animals show a marked decrease in locomotory behaviour, resulting from both a reduction in number and rate of peristaltic contractions. Inhibition of neurotransmitter release, using targeted expression of tetanus toxin light chain (TeTxLC), in the slowmo neurons marked by an enhancer-trap results in a similar phenotype of largely absent or uncoordinated contractions. Cloning of the slowmo gene identifies a product related to a family of proteins of unknown function. We show that Slowmo is associated with mitochondria, indicative of it being a mitochondrial protein, and that during embryogenesis and early larval development is restricted to the nervous system in a subset of cells. The enhancer-trap marks a cellular component of the CNS that is seemingly required to regulate peristaltic movement. 相似文献
17.
Stefanie Kimbacher Ingrid Gerstl Branko Velimirov Sylvia Hagemann 《Molecular genetics and genomics : MGG》2009,282(2):165-172
P transposons belong to the eukaryotic DNA transposons, which are transposed by a cut and paste mechanism using a P-element-coded transposase. They have been detected in Drosophila, and reside as single copies and stable homologous sequences in many vertebrate species. We present the P elements Pcin1, Pcin2 and Pcin3 from Ciona intestinalis, a species of the most primitive chordates, and compare them with those from Ciona savignyi. They showed typical DNA transposon structures, namely terminal inverted repeats and target site duplications. The coding
region of Pcin1 consisted of 13 small exons that could be translated into a P-transposon-homologous protein. C. intestinalis and C. savignyi displayed nearly the same phenotype. However, their P elements were highly divergent and the assumed P transposase from C. intestinalis was more closely related to the transposase from Drosophila melanogaster than to the transposase of C. savignyi. The present study showed that P elements with typical features of transposable DNA elements may be found already at the base of the chordate lineage.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
18.
Subramanian Mayavan Kondeti Subramanyam Balusamy Jaganath Dorairaj Sathish Markandan Manickavasagam Andy Ganapathi 《Plant cell reports》2015,34(10):1835-1848
Key message
An efficient, reproducible, and genotype-independent in planta transformation has been developed for sugarcane using setts as explant.Abstract
Traditional Agrobacterium-mediated genetic transformation and in vitro regeneration of sugarcane is a complex and time-consuming process. Development of an efficient Agrobacterium-mediated transformation protocol, which can produce a large number of transgenic plants in short duration is advantageous. Hence, in the present investigation, we developed a tissue culture-independent in planta genetic transformation system for sugarcane using setts collected from 6-month-old sugarcane plants. The sugarcane setts (nodal cuttings) were infected with three Agrobacterium tumefaciens strains harbouring pCAMBIA 1301–bar plasmid, and the transformants were selected against BASTA®. Several parameters influencing the in planta transformation such as A. tumefaciens strains, acetosyringone, sonication and exposure to vacuum pressure, have been evaluated. The putatively transformed sugarcane plants were screened by GUS histochemical assay. Sugarcane setts were pricked and sonicated for 6 min and vacuum infiltered for 2 min at 500 mmHg in A. tumefaciens C58C1 suspension containing 100 µM acetosyringone, 0.1 % Silwett L-77 showed the highest transformation efficiency of 29.6 % (with var. Co 62175). The three-stage selection process completely eliminated the chimeric transgenic sugarcane plants. Among the five sugarcane varieties evaluated using the standardized protocol, var. Co 6907 showed the maximum transformation efficiency (32.6 %). The in planta transformation protocol described here is applicable to transfer the economically important genes into different varieties of sugarcane in relatively short time.19.
20.