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1.
James J. McGrath Wing C. Wong J. Danny Cooley David C. Straus 《Current microbiology》1999,38(1):33-36
Buildings with indoor air quality (IAQ) complaints frequently have high airborne concentrations of Penicillium species, while buildings with few IAQ complaints have an indoor air (IDA) fungal ecology similar to outdoor air (ODA), where
Cladosporium species is usually the dominant microorganism. These studies compared fungal air profiles, measured continually over 6 h
in a documented sick building, in IDA in a room experiencing IAQ problems with fungal profiles measured concurrently in ODA.
The dominant species collected at both sites were Penicillium species, Cladosporium species, and Alternaria species. In the IDA, Penicillium species were always the dominant organisms, ranging from 150 to 567 cfu/m3 (89.8–100% of the total fungi). In the ODA, Cladosporium species were dominant in four samples (40.0–70.6%), while Penicillium species were dominant (52.7–79.6%) in two. These data demonstrate that, even though ODA fungal profiles are changing continuously,
IDA fungal profiles in “sick” buildings tend to remain unchanged.
Received: 6 July 1998 / Accepted: 12 August 1998 相似文献
2.
Species of Aspergillus were the most prevalent fungi isolated from 51 samples of damaged flue-cured tobacco of the 1966 U.S. crop, comprising 57% of the total isolates. Other prevalent fungi were Penicillium (16%), Alternaria (8%), Cladosporium (4%), and Chaetomium (4%). Members of the Aspergillus glaucus group were isolated most frequently from samples with moisture contents ranging from 18 to 28%, whereas Alternaria, Cladosporium, and Penicillium were isolated consistently from samples containing 24 to 32% moisture. Aspergillus niger was prevalent in tobacco ranging in moisture content from 18 to 30%. 相似文献
3.
Thirty homes of asthmatic adults located in Mexico City were examined to determine the predominant culturable fungi and the
changes in their airborne concentrations. Fungi were cultured and identified microscopically from air samples collected in
naturally ventilated homes, during both wet (July–August) and cool dry (November–December) seasons, and from settled dust
from the same homes. Airborne dust from indoor yielded 99–4950 cfu m−3, and settled dust 102–106 cfu g−1 on DG18 agar. The indoor geometric mean concentration of airborne fungi during the cool dry season was 460 cfu m−3 while in the wet season it was 141 cfu m−3. Similarly, numbers of airborne fungal propagules out of doors decreased 60% between the dry and wet season. In general,
the total fungal concentrations in indoor air were less than 103 cfu m−3 and a large proportion of them was collected in Stage-2 of the Andersen sampler. Moreover, the ratio between indoor and outdoor
concentrations was <3:1. Five of the 30 sampled homes yielded >500 cfu m−3 of one genus, with up to 1493Cladosporium cfu m−3 or 2549Penicillium cfu m−3. Also, these two genera were predominant in both airborne and settled dust, and their concentrations were greater indoors
than out, indicating a possible indoor source of fungal propagules. The predominant species wereCladosporium herbarum, Penicillium aurantiogriseum andP. chrysogenum. These results suggest that exposure to large concentrations of fungi occurs indoors and is associated with both seasons
of the year and with particular home characteristics. 相似文献
4.
The objective was to determine potential exposure to airborne fungi in greenhouses and to characterize the temporal patterns
of airborne fungi in relation to environmental conditions. We analyzed air samples collected in two greenhouses. Results showed
that the top 5 fungi in greenhouse 1 were Trichoderma, hyphal fragments, Aspergillus/Penicillium-like, Cladosporium, and Botrytis in a descending order. Those in greenhouse 2 were Aspergillus/Penicillium-like, Cladosporium, Botrytis, yeast-like, and hyphal fragments. Maximum concentrations of Trichoderma and total spores in greenhouse 1 were 36,426 and 49,729 spores/m3, respectively. Maximum concentrations of Aspergillus/Penicillium and total spores in greenhouse 2 were 46,961 and 71,037 spores/m3, respectively. Airborne fungal populations fluctuated dramatically within 2 h during work hours, tenfold for Aspergillus/Penicillium, 66-fold for Trichoderma, and sevenfold for total spores. QPCR detected Trichoderma harzianum ranging from 7 to 3,500 conidia E/m3. Aspergillus/Penicillium and Botrytis showed diurnal patterns, but not Trichoderma. Aspergillus/Penicillium and Cladosporium were positively correlated with temperature, relative humidity, dew point, heat index, and light and negatively with air
movement and air pressure. Botrytis and Trichoderma were not correlated with the environmental factors. Greenhouse workers were potentially exposed up to 71,037 spores/m3 of airborne fungi. 相似文献
5.
Donát Magyar Zoltán Kállai Matthias Sipiczki Csaba Dobolyi Flóra Sebők Tímea Beregszászi Zoltán Bihari László Kredics Gyula Oros 《Aerobiologia》2018,34(2):171-185
The composition of fungal biota and air quality of five traditional subterranean wine cellars and one store building of a modern wine production facility were examined in the Tokaj wine region (northeastern Hungary). Air samples were collected with SAS IAQ sampler onto PDA, MEA and RBA. Strains representing morphotypes were isolated from colonies formed on agar plates from either air or surface samples. The internal transcribed spacer (ITS) region of the rRNA gene cluster was amplified with primers ITS1 and ITS4. Altogether 90 morphotypes were isolated, 48 and 12 strains (43 species) from the air and surfaces, respectively. The number of spore-forming species generated high diversity of indoor fungi and differences between the cellars’ fungal compositions; however, their dominant species were proved to be the same. Among the isolated strains Penicillium spp. were the most frequent. The walls of cellars were covered by colonies of Zasmidium (Cladosporium) cellare often referred to as a noble mold. Even so, this mold has been found only at a small concentration in the air samples (10–30 CFU/m3). The walls of the modern store were free of molds. Diversity of fungi of the examined wine cellars was influenced by environmental conditions to a certain degree, such as elevation (height above sea level), age, reconstruction time of cellars, indoor ethanol concentration and the number of chimneys. The location of cellars poorly influenced the concentration of fungi of the air inside cellars, contrary to outdoors where the air of the municipal area contained more CFUs than that of rural spaces. 相似文献
6.
Concentration and distribution characteristics of airborne fungi in indoor and outdoor air of Tehran subway stations 总被引:1,自引:0,他引:1
Mohammad Hoseini Hosein Jabbari Kazem Naddafi Ramin Nabizadeh Mohammad Rahbar Masoud Yunesian Jalil Jaafari 《Aerobiologia》2013,29(3):355-363
The concentration and distribution characteristics of airborne fungi were investigated in indoor and outdoor air of two metro stations (Imam Khomeini and Sadeghiyeh stations) in Tehran subway. Samples were taken from indoor air at each station from platform and ticket office area also from adjacent outdoor air of each station. Indoor sampling was conducted for two types of trains, old and new. The concentration of airborne fungi ranged from 21 CFU/m3 at the outdoor air of Imam Khomeini station to 1,402 CFU/m3 in the air samples collected from the platform of this station. Results showed that airborne fungi concentrations at indoor air were higher than the outdoor air (p < 0.05), and fungal levels significantly correlated with the number of passengers (p < 0.05; r = 0.68) and RH % (p < 0.05; r = 0.43). Sixteen genera of fungi were isolated in all sampled environments. The predominant genera identified in indoor and outdoor air were Penicillium spp. (34.88 % of total airborne fungi) and Alternaria spp. (29.33 % of total airborne fungi), respectively. The results of this study showed that the indoor air quality in subway is worse than the outdoor air. 相似文献
7.
Fungal agents are responsible for a variety of respiratory diseases both in humans and animals. The nature and seasonal variations of fungi have been investigated in many environments with wide ranging results. The aims of the present report were (i) to evaluate the quality and magnitude of exposure to airborne fungi in three differently structured equine stalls (open air, partially and completely enclosed buildings) during a one-year period, using an air sampling technique and (ii) to compare the distribution and frequency of fungal species, with regards to these different environments. Air samples were collected monthly from December 2001 to November 2002 by means of a surface air sampler (SAS) Super-90, (PBI International, Milan, Italy). Penicillium and Aspergillus spp. were cultured from all the stables in all seasons. Mucoraceae were also recovered in all seasons in stalls 1 and 2, while they were not isolated in spring and fall in stall 3. These fungi were detected in 28.4%, 72.9% and 60.5% of the total number of samples, respectively. Other fungal genera such as Alternaria, Cladosporium, Fusarium, Beauveria and Drechslera were also occasionally recovered.Viable fungal concentrations varied greatly, ranging from below the limit of detection to more than 3000 CFU/m3 for stables 1 and 2, and 1750 CFU/m3 for stable 3. The median fungal concentration was approximately 178 CFU/m3. Total fungal concentration appeared to be highest in summer, winter and spring, and lowest in the fall. 相似文献
8.
Tatiana Barkova Paul Comtois Zave Chad John Weisnagel André Caron Jaime Del Carpio Guérin Dorval John Schulz Lorne Umemoto Yves Charbonneau David Copeland 《Aerobiologia》1995,11(2):119-128
The purpose of this epidemiological study was to assess respiratory allergy in relation to the presence of indoor airborne
fungi. The relationship between IgE-mediated respiratory allergy (skin test positivity) and the presence of fungi (CFU/m3) in the indoor environments of 104 subjects was assessed in a cross-sectional study by controlling for extraneous variables
(age, gender, predisposition, asthma, rhinitis, skin positivity to ragweed and mite, and smoking). The qualitative and quantitative
measurements of airborne seasonal fungi (Alternaria spp. andCladosporium spp.) and non-seasonal airborne fungi (Penicillium spp. andAspergillus spp.) were taken in the subjects’ indoor environments twice in a 2-year period by volumetric methods (Burkard Personal Sampler).
There was a significant association between skin test positivity to seasonal fungi and to ragweed (Adj. OR=3.42, CI=1.76–6.66).
There was no association between skin test positivity to seasonal fungi and asthma (Adj. OR=0.52, CI=0.28–0.98), but a significant
association was found between skin test positivity to seasonal fungi and rhinitis (Adj. OR=5, CI=2.03–12.32). In a logistic
regression analysis (maximum likelihood estimates—model A), no statistical association was found indoors between skin prick
test positivity to seasonal fungi (Alternaria and/orCladosporium) and airborneAlternaria and/orCladosporium concentrations (Adj. OR=1.18, CI=0.66–2.07). There was a significant association between skin prick test positivity to seasonal
fungi and to non-seasonal fungi (Adj. OR=12.81, CI=1.67–98.34). There was no association between asthma and airbornePenicillium concentrations (Adj. OR=1.86, CI=0.47–7.33) nor between rhinitis and airbornePenicillium concentrations (Adj. OR=0.18, CI=0.03–1.19). In another logistic regression analysis (maximum likelihood estimates — model
B) using non-seasonal fungi (Aspergillus andPenicillium), no statistical association was found indoors between skin prick test positivity to non-seasonal fungi and airbornePenicillium concentrations (Adj. OR=0.33, CI=0.07–1.69). These findings suggest an association between rhinitis and seasonal fungi. In
the rhinitis stratum, subjects who had skin test positivity to ragweed had a higher risk of being sensitive to seasonal airborne
fungal allergens. Subjects with non-seasonal fungal allergy had a high relative risk if they were also allergic to seasonal
fungi. There was no association between asthma and airborne fungi, as the epidemiological study (cross-sectional design),
by definition, does not allow an etiological evaluation of chronic disease. This would require a longitudinal study, i.e.
the measurement of repeated exposure as an independent variable (allergen) and repeated measurement as a function of the disease
as outcome in humans as a dependent variable. 相似文献
9.
《Revista iberoamericana de micología》2014,31(3):182-187
BackgroundThe high relative humidity and temperatures in tropical countries create favorable conditions for development of fungi that are not only a risk to human health but they can also colonize documentary support.AimsTo study the concentration of the airborne fungi in two repositories of the National Archives of the Republic of Cuba, the mycobiota deposited on different photographic supports and maps preserved in these repositories, and to determine the taxonomic characterization of the fungi isolated.MethodsThe air sampling was performed using a sedimentation method, and the supports (6 pictures and 7 maps) were analyzed using moistened sterile swabs.ResultsThe Cladosporium genus was predominant, followed by Aspergillus and Penicillium genera. Filamentous fungi were isolated in all the photos and maps, and yeasts were only isolated from a photographic supports and a map. We identified several species of Aspergillus and Penicillium genera, but Aspergillus niger and Aspergillus flavus predominated. Candida and Rhodotorula were the yeast genera isolated.ConclusionsThe fungal concentration of the air demonstrated that the environments were not contaminated. From the 26 species of filamentous fungi isolated only 5 were detected in the indoor air of the repositories and on one or more of the document supports analyzed (representing a 19.3%). This shows that there is a low ecological relationship between the fungi detected in the indoor air and those that were isolated from the supports studied. 相似文献
10.
Microbial population dynamics on Golden Delicious apples from bud to harvest and effect of fungicide applications 总被引:3,自引:0,他引:3
The microbial population dynamics on apples cv. Golden Delicious were analysed every 15 days between bud and harvest in a fully replicated experiment in northern Spain in 1994 and 1995. The total microbial populations varied with developmental stage, and with prevailing climatic conditions. The predominant mycroflora were the filamentous fungi Cladosporium and Alternaria spp. and white and pink yeasts. Other genera isolated included mainly species of Epicoccum, Fusarium and Acremonium. However, the most important post-harvest pathogens Penicillium expansum and Botrytis cinerea were seldom isolated from ripening apples. Maximum total filamentous fungal populations occurred after fruit set and during early ripening [2 × 104cfu (colony-forming units) g-1 approximately] while those of bacteria were maximum at bud stage (3.5 × 105and 3.0 × 104 cfu g-1 in 1994 and 1995 respectively). White yeasts were more numerous than pink yeasts. Endophytic infection of apple buds by Alternaria spp., responsible for core rot, was found in almost all bud tissue. By contrast, Cladosporium spp. were initially isolated later from 12.5–50% of tissue samples during blooming and fruit set. The impact of a four-spray fungicide regime during apple development significantly decreased the total filamentous fungal populations in both years, and that of Cladosporium spp. in 1994. However, bacterial populations were often higher on apples from fungicide-treated plots. Fungicide sprays decreased populations of Cladosporium, Alternaria and white yeasts for a maximum of up to 15–30 days after application. Fungicide application had little effect on endophytic infection of apples by Alternaria spp. between bud and harvest. 相似文献
11.
Rafał Ogórek Wojciech Pusz Paweł P. Zagożdżon Bartosz Kozak Henryk Bujak 《Geomicrobiology journal》2017,34(10):823-833
This paper is a speleomycological report from a former aluminous shale mine in Janowiec, Poland. Fungi were identified morphologically and molecularly. Microclimatic conditions differed significantly between locations of the study. However, the external environment around the mine did not directly increase the community composition and concentration of fungi in the mine. The density of fungi isolated from the air outside the mine was 63.1 colony forming units (CFU) per 1 cm3 of air. Inside the mine, fungal density ranged from 287.5 to 655 CFU per 1 m3 from the air, 28.4 to 131.1 CFU per 1 cm2 from the rock surfaces and 288.1 to 335.1 CFU per 1 cm3 from the water. Pearson correlation analysis showed that the levels of fungi isolated from the air were correlated positively with temperature, relative humidity and CO2 concentration. The concentration of fungi isolated from the rock surfaces showed a positive correlation with air flow. Five species of filamentous fungi were isolated from the sampled external air, 10 species from the internal air, six species from the rock surface and 11 species from the water. The fungi most frequently isolated from the air and water of the mine belonged to Penicillium spp., whereas from the rock surface, Geomyces pannorum was most frequently isolated. Some of the fungi present in the mine can be psychrotolerant and pathogenic for humans and animals, and they can also cause degradation of rocks. 相似文献
12.
The fungal population of freshly harvested strawberries, raspberries and blackberries was studied throughout the fruiting seasons and the relative importance of fungi responsible for spoilage of stored fruit from different harvest dates was assessed. Botrytis cinerea, Cladosporium spp., Penicillium spp., Aureobasidium pullulans and Mucor spp. were consistently present on all three fruits while Colletotrichum gloeosporioides and Didymella applanata were prevalent on raspberries only and Epicoccum purpurascens and Alternaria spp. on blackberries only. Botrytis cinerea and Mucor mucedo were the predominant spoilage organisms of all three fruits, although the relative importance of the two fungi changed as the season progressed, especially for strawberries. Rhizopus stolonifer, Cladosporium spp., Penicillium spp. and Colletotrichum gloeosporioides were only of minor importance as spoilage organisms. 相似文献
13.
The number and composition of opportunistic microscopic fungi was studied in soils and surface air (0.2 and 1.5 m above the
surface) in the megalopolis districts (Tushino, Moscow) of different age of construction (6 and 40 years) and in urban recreational
forests. The highest number (up to 1500 CFU/m3) determined by plating from air was found in the summer in new-built quarters. Direct count of fungal diaspores in airborne
dust yielded significantly higher values (up to 4 × 105/m3). The composition of the soil fungal population differed significantly from that of the air. In soil, the diversity of potentially
pathogenic fungi was higher, while in air, their abundance was greater. The highest content of opportunistic fungi in soil
and air was observed in spring and late summer-autumn, respectively. The fungi known as allergenic (mostly Cladosporium spp.) predominate in the air in autumn, especially in the new-built quarters. 相似文献
14.
《International biodeterioration & biodegradation》2005,55(2):85-91
A comparative study of the occurrence of culturable airborne fungi was carried out during the four seasons of the year in four social welfare homes (SWHs), one in each of the cities of Cracow (Małopolskie province) and Białystok (Podlaskie province) and the villages of Karniowice and Czerewki situated approximately 20 km from the two cities respectively. Air of randomly chosen rooms and bathrooms, corridors, ward kitchens, soiled-linen closets, dining rooms, day rooms and nurses’ stations was sampled using single-stage impactor samplers and isolated moulds and yeasts were identified by macroscopic, microscopic and biochemical characteristics. Generally the greatest numbers of fungi were observed in the autumn. Penicillium and Cladosporium were isolated from the air in all four SWHs during all seasons of the year, but a wider range of other taxa were isolated from those in Małopolskie province. 相似文献
15.
The more the mold species isolated on a culture medium, the more the sampling environment is represented accurately. According to the sampling purpose, it is crucial to use the best culture medium for mold. However, no study is available regarding the comparison of dichloran rose bengal chloramphenicol (DRBC) and Sabouraud dextrose agar with cycloheximide and chloramphenicol (SDA-CHX-CHL) culture media in terms of their application for airborne sampling, isolation, and identification of fungi. Airborne mold samples were impacted onto both DRBC and SDA-CHX-CHL, simultaneously using single-stage Andersen sampler. The limit of detection (LOD) value for airborne mold count was 7 CFU m?3 (1 colony growth on the Petri dish). The total mold counts (TMC) ranged between <7 and 504 CFU m?3 (med 56 CFU m?3) and <7 and 1218 CFU m?3 (med 259 CFU m?3), collected on SDA-CHX-CHL and DRBC, respectively. Significantly higher TMC were observed on DRBC than on SDA regardless of the sampling environment (i.e, indoor or outdoor) (p < 0.05). Among the most predominant mold genera, observation frequencies of Penicillium spp. and Aspergillus spp. on both culture media were found to be more than 70%. Observation frequencies of Cladosporium spp., Alternaria spp., and yeast were found to be higher in samples collected on DRBC than those on SDA-CHX-CHL. Finally, DRBC was found to be superior to SDA in terms of both number of colonies and number of genera isolated from the air. 相似文献
16.
Rafał Ogórek Wojciech Pusz Krzysztof Matkowski Elżbieta Pląskowska 《Geomicrobiology journal》2014,31(10):900-906
Our study is the first mycological evaluation of the air and the rocks in the underground Rzeczka complex in Sowie Mountains (Lower Silesia, Poland). The density of filamentous fungi isolated from the air inside and outside the adit ranged between 245.5 to 1332.6 Colony Forming Units in 1 m3 of air. Sixteen species of filamentous fungi were isolated from the internal air sampled and 11 from the outside of the adit. Cladosporium spp. were the fungi most frequently isolated from the internal atmosphere of the adit and from the external air. From the rock walls and from the rock debris on the floor of the adit only seven species of fungi were isolated. The fungi most frequently isolated from the rock walls were the Aspergillus niger group and from the rock debris we also found A. niger group along with species of Mucor. The concentration of airborne fungi in the adit did not exceed official limits and norms present for a health risk to the tourists, but fungi species isolated from the rocks can cause their degradation. 相似文献
17.
Fungal Colonization of Air Filters and Insulation in a Multi-Story Office Building: Production of Volatile Organics 总被引:1,自引:0,他引:1
D.G. Ahearn S.A. Crow R.B. Simmons D.L. Price S.K. Mishra D.L. Pierson 《Current microbiology》1997,35(5):305-308
Secondary air filters in the air-handling units on four floors of a multi-story office building with a history of fungal
colonization of insulation within the air distribution system were examined for the presence of growing fungi and production
of volatile organic compounds. Fungal mycelium and conidia of Cladosporium and Penicillium spp. were observed on insulation from all floors and both sides of the air filters from one floor. Lower concentrations of
volatile organics were released from air filter medium colonized with fungi as compared with noncolonized filter medium. However,
the volatiles from the colonized filter medium included fungal metabolites such as acetone and a carbonyl sulfide-like compound
that were not released from noncolonized filter medium. The growth of fungi in air distribution systems may affect the content
of volatile organics in indoor air.
Received: 2 June 1997 / Accepted: 13 June 1997 相似文献
18.
G. D. Sharma 《Folia microbiologica》1981,26(4):321-327
The effect of man-made fire on soil mierobial population in a natural subtropical pine forest eco-system, was studied. A nearly fifty years old pine(Pinus kesiya Royle) forest was cut and burnt in March 1977. Another half of this pine forest was left uncut and unburnt. Microbial population was destroyed completely just after the burning and recolonization occurred after some days. Bacteria and actinomycetes were found to be the first colonizers followed by fungi. Burning initiated better growth and higher population of bacteria after two or three showers. Bacterial population was highest in July in burnt and in May in unburnt forest. Maximum fungal population in burnt site was recorded in July while it was highest in April and May in unburnt site.Penicillium spp.,Cladosporium sp. andTrichoderma sp. were found to be first colonizing fungi just after the burning. In unburnt forest eco-systemAbsidia sp.,Trichoderma sp.,Fusarium sp. andPenicillium spp. were found to be dominant. 相似文献
19.
Frank J. J. Segers Martin Meijer Jos Houbraken Robert A. Samson Han A. B. W?sten Jan Dijksterhuis 《PloS one》2015,10(12)
Indoor fungi are a major cause of cosmetic and structural damage of buildings worldwide and prolonged exposure of these fungi poses a health risk. Aspergillus, Penicillium and Cladosporium species are the most predominant fungi in indoor environments. Cladosporium species predominate under ambient conditions. A total of 123 Cladosporium isolates originating from indoor air and indoor surfaces of archives, industrial factories, laboratories, and other buildings from four continents were identified by sequencing the internal transcribed spacer (ITS), and a part of the translation elongation factor 1α gene (TEF) and actin gene (ACT). Species from the Cladosporium sphaerospermum species complex were most predominant representing 44.7% of all isolates, while the Cladosporium cladosporioides and Cladosporium herbarum species complexes represented 33.3% and 22.0%, respectively. The contribution of the C. sphaerospermum species complex was 23.1% and 58.2% in the indoor air and isolates from indoor surfaces, respectively. Isolates from this species complex showed growth at lower water activity (≥ 0.82) when compared to species from the C. cladosporioides and C. herbarum species complexes (≥ 0.85). Together, these data indicate that xerotolerance provide the C. sphaerospermum species complex advantage in colonizing indoor surfaces. As a consequence, C. sphaerospermum are proposed to be the most predominant fungus at these locations under ambient conditions. Findings are discussed in relation to the specificity of allergy test, as the current species of Cladosporium used to develop these tests are not the predominant indoor species. 相似文献
20.
Erina Kohata Rui Kano Yuichiro Akune Yoshito Ohno Makoto Soichi Tokuma Yanai Atsuhiko Hasegawa Hiroshi Kamata 《Mycopathologia》2013,176(5-6):403-408
Systemic mycoses in killer whales (Orcinus orca) are rare diseases, but have been reported. Two killer whales died by fungal infections at the Port of Nagoya Public Aquarium in Japan. In this study, the fungal flora of the pool environment at the aquarium was characterized. Alternaria spp., Aspergillus spp. (A. fumigatus, A. niger, A. versicolor), Fusarium spp. and Penicillium spp. were isolated from the air and the pool surroundings. The other isolates were identified as fungal species non-pathogenic for mammals. However, the species of fungi isolated from the environmental samples in this study were not the same as those isolated from the cases of disease in killer whales previously reported. 相似文献